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Q8CFX1

- G6PE_MOUSE

UniProt

Q8CFX1 - G6PE_MOUSE

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Protein

GDH/6PGL endoplasmic bifunctional protein

Gene

H6pd

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.By similarity

Catalytic activityi

Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.PROSITE-ProRule annotation
D-glucose 6-phosphate + NAD(P)+ = D-glucono-1,5-lactone 6-phosphate + NAD(P)H.
6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311NADPBy similarity
Binding sitei63 – 631NADPBy similarity
Binding sitei201 – 2011SubstratePROSITE-ProRule annotation
Binding sitei205 – 2051SubstratePROSITE-ProRule annotation
Active sitei264 – 2641Proton acceptorBy similarity

GO - Molecular functioni

  1. 6-phosphogluconolactonase activity Source: MGI
  2. glucose 1-dehydrogenase [NAD(P)] activity Source: UniProtKB-EC
  3. glucose-6-phosphate dehydrogenase activity Source: MGI
  4. NADP binding Source: InterPro

GO - Biological processi

  1. pentose-phosphate shunt Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

SABIO-RKQ8CFX1.

Names & Taxonomyi

Protein namesi
Recommended name:
GDH/6PGL endoplasmic bifunctional protein
Including the following 2 domains:
Glucose 1-dehydrogenase (EC:1.1.1.47)
Alternative name(s):
Hexose-6-phosphate dehydrogenase
6-phosphogluconolactonase (EC:3.1.1.31)
Short name:
6PGL
Gene namesi
Name:H6pd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2140356. H6pd.

Subcellular locationi

Endoplasmic reticulum lumen By similarity
Note: Microsomes, endoplasmic reticulum lumen.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 789773GDH/6PGL endoplasmic bifunctional proteinPRO_0000236794Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171Pyrrolidone carboxylic acidBy similarity
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
Modified residuei205 – 2051N6-succinyllysine1 Publication
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
Modified residuei424 – 4241N6-succinyllysine1 Publication
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiQ8CFX1.
PaxDbiQ8CFX1.
PRIDEiQ8CFX1.

PTM databases

PhosphoSiteiQ8CFX1.

Expressioni

Gene expression databases

BgeeiQ8CFX1.
ExpressionAtlasiQ8CFX1. baseline and differential.
GenevestigatoriQ8CFX1.

Interactioni

Protein-protein interaction databases

IntActiQ8CFX1. 3 interactions.
MINTiMINT-1837869.

Structurei

3D structure databases

ProteinModelPortaliQ8CFX1.
SMRiQ8CFX1. Positions 25-500, 559-786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 524508Glucose 1-dehydrogenaseAdd
BLAST
Regioni525 – 53814LinkerAdd
BLAST
Regioni539 – 7892516-phosphogluconolactonaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0363.
GeneTreeiENSGT00530000063435.
HOGENOMiHOG000231077.
HOVERGENiHBG005780.
InParanoidiQ8CFX1.
KOiK13937.
OrthoDBiEOG7BW0HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005900. 6-phosphogluconolactonase_DevB.
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR006148. Glc/Gal-6P_isomerase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PF01182. Glucosamine_iso. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR01198. pgl. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFX1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLAAMCLAL LGCLQAQELK GHVSIILLGA TGDLAKKYLW QGLFQLYLDE
60 70 80 90 100
AGKGHSFSFH GAALTAPQQG QKLMDKVLES LSCPKDLVPS RCDELKGQFL
110 120 130 140 150
QLSQYRQLKT VEDYQTLNKD IETQVQQDGL WEAGRIFYFS VPPFAYADIA
160 170 180 190 200
RNINSSCRPH PGAWLRVVFE KPFGHDHLSA QQLASELGSF FQEEEMYRVD
210 220 230 240 250
HYLGKQAVAQ ILPFRDQNRK ALDGLWNRHH VERVEIILKE TIDAEGRASF
260 270 280 290 300
YEEYGVIRDT LQNHLTEILT LVAMELPLNI SSSAAVLQHK LWAFQALRGL
310 320 330 340 350
QKSSAILGQY QAYSGQVRRE LQKPDGFQSL TPTFAGVLVH IDNLRWEGVP
360 370 380 390 400
FILMSGKALD ERVGYVRIVF KNRAYCTQSE RHWVPEQSRC LPQQIIFYIG
410 420 430 440 450
HGELGHPAIL VSRNLFKPSL PTQKWKEVQD QPGLRLFGRP LSDYYAYRPV
460 470 480 490 500
REQDAYSTLL SHIFHCRKES FITTENLLAS WVFWTPLLDS LAFEVPRPYP
510 520 530 540 550
GGAENGQLLD FEFSGGQLTF SQQQLEVLIP DLGSVPKPSD FQVLGARYRQ
560 570 580 590 600
SPLITAWPEE LISKLASDIE AAAVQAVRHF GKFHLALSGG SSPIALFQQL
610 620 630 640 650
ATGHYSFPWA HTHLWLVDER CVPLSDPDSN FQGLQAHLLQ HVRVPYYNIH
660 670 680 690 700
PMPVHLHQRL CAEEDQGAQT YASEISALVA NSSFDLVLLG MGTDGHTASL
710 720 730 740 750
FPQSPTGLDG DQLVVLTESP FRPHQRMSLS LPLINRAKKV AVLVMGRTKR
760 770 780
EITTLVSRVG HEPKKWPISG VVPLSGQLVW YMDYEAFLG
Length:789
Mass (Da):88,928
Last modified:July 27, 2011 - v2
Checksum:iE970CE8B35D3E1E5
GO

Sequence cautioni

The sequence BAC32260.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771V → A in BAE35029. (PubMed:16141072)Curated
Sequence conflicti77 – 771V → A in CAQ51682. (PubMed:19468303)Curated
Sequence conflicti77 – 771V → A in AAH42677. (PubMed:15489334)Curated
Sequence conflicti106 – 1061R → H in BAE35029. (PubMed:16141072)Curated
Sequence conflicti106 – 1061R → H in CAQ51682. (PubMed:19468303)Curated
Sequence conflicti106 – 1061R → H in AAH42677. (PubMed:15489334)Curated
Sequence conflicti285 – 2851A → T in BAE35029. (PubMed:16141072)Curated
Sequence conflicti285 – 2851A → T in CAQ51682. (PubMed:19468303)Curated
Sequence conflicti285 – 2851A → T in AAH42677. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK045199 mRNA. Translation: BAC32260.1. Different initiation.
AK159373 mRNA. Translation: BAE35029.1.
AL606914 Genomic DNA. Translation: CAM16119.1.
CU463327 Genomic DNA. Translation: CAQ51682.1.
BC042677 mRNA. Translation: AAH42677.1.
CCDSiCCDS71524.1.
RefSeqiNP_001277933.1. NM_001291004.1.
NP_775547.2. NM_173371.4.
XP_006537124.1. XM_006537061.1.
XP_006538524.1. XM_006538461.1.
UniGeneiMm.22183.

Genome annotation databases

EnsembliENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980.
GeneIDi100198.
KEGGimmu:100198.
UCSCiuc008vxh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK045199 mRNA. Translation: BAC32260.1 . Different initiation.
AK159373 mRNA. Translation: BAE35029.1 .
AL606914 Genomic DNA. Translation: CAM16119.1 .
CU463327 Genomic DNA. Translation: CAQ51682.1 .
BC042677 mRNA. Translation: AAH42677.1 .
CCDSi CCDS71524.1.
RefSeqi NP_001277933.1. NM_001291004.1.
NP_775547.2. NM_173371.4.
XP_006537124.1. XM_006537061.1.
XP_006538524.1. XM_006538461.1.
UniGenei Mm.22183.

3D structure databases

ProteinModelPortali Q8CFX1.
SMRi Q8CFX1. Positions 25-500, 559-786.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8CFX1. 3 interactions.
MINTi MINT-1837869.

PTM databases

PhosphoSitei Q8CFX1.

Proteomic databases

MaxQBi Q8CFX1.
PaxDbi Q8CFX1.
PRIDEi Q8CFX1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000084117 ; ENSMUSP00000081134 ; ENSMUSG00000028980 .
GeneIDi 100198.
KEGGi mmu:100198.
UCSCi uc008vxh.1. mouse.

Organism-specific databases

CTDi 9563.
MGIi MGI:2140356. H6pd.

Phylogenomic databases

eggNOGi COG0363.
GeneTreei ENSGT00530000063435.
HOGENOMi HOG000231077.
HOVERGENi HBG005780.
InParanoidi Q8CFX1.
KOi K13937.
OrthoDBi EOG7BW0HS.

Enzyme and pathway databases

SABIO-RK Q8CFX1.

Miscellaneous databases

NextBioi 354313.
PROi Q8CFX1.
SOURCEi Search...

Gene expression databases

Bgeei Q8CFX1.
ExpressionAtlasi Q8CFX1. baseline and differential.
Genevestigatori Q8CFX1.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR005900. 6-phosphogluconolactonase_DevB.
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR006148. Glc/Gal-6P_isomerase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PF01182. Glucosamine_iso. 1 hit.
[Graphical view ]
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR01198. pgl. 1 hit.
PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-205 AND LYS-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiG6PE_MOUSE
AccessioniPrimary (citable) accession number: Q8CFX1
Secondary accession number(s): A2A7A9, B2KGW7, Q8BLH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3