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Q8CFX1

- G6PE_MOUSE

UniProt

Q8CFX1 - G6PE_MOUSE

Protein

GDH/6PGL endoplasmic bifunctional protein

Gene

H6pd

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.By similarity

    Catalytic activityi

    Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.PROSITE-ProRule annotation
    D-glucose 6-phosphate + NAD(P)+ = D-glucono-1,5-lactone 6-phosphate + NAD(P)H.
    6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311NADPBy similarity
    Binding sitei63 – 631NADPBy similarity
    Binding sitei201 – 2011SubstratePROSITE-ProRule annotation
    Binding sitei205 – 2051SubstratePROSITE-ProRule annotation
    Active sitei264 – 2641Proton acceptorBy similarity

    GO - Molecular functioni

    1. 6-phosphogluconolactonase activity Source: MGI
    2. glucose 1-dehydrogenase [NAD(P)] activity Source: UniProtKB-EC
    3. glucose-6-phosphate dehydrogenase activity Source: MGI
    4. NADP binding Source: InterPro

    GO - Biological processi

    1. pentose-phosphate shunt Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    SABIO-RKQ8CFX1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GDH/6PGL endoplasmic bifunctional protein
    Including the following 2 domains:
    Glucose 1-dehydrogenase (EC:1.1.1.47)
    Alternative name(s):
    Hexose-6-phosphate dehydrogenase
    6-phosphogluconolactonase (EC:3.1.1.31)
    Short name:
    6PGL
    Gene namesi
    Name:H6pd
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2140356. H6pd.

    Subcellular locationi

    Endoplasmic reticulum lumen By similarity
    Note: Microsomes, endoplasmic reticulum lumen.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 789773GDH/6PGL endoplasmic bifunctional proteinPRO_0000236794Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei17 – 171Pyrrolidone carboxylic acidBy similarity
    Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
    Modified residuei205 – 2051N6-succinyllysine1 Publication
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Modified residuei424 – 4241N6-succinyllysine1 Publication
    Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiQ8CFX1.
    PaxDbiQ8CFX1.
    PRIDEiQ8CFX1.

    PTM databases

    PhosphoSiteiQ8CFX1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CFX1.
    BgeeiQ8CFX1.
    GenevestigatoriQ8CFX1.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8CFX1. 3 interactions.
    MINTiMINT-1837869.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFX1.
    SMRiQ8CFX1. Positions 25-500, 554-786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni17 – 524508Glucose 1-dehydrogenaseAdd
    BLAST
    Regioni525 – 53814LinkerAdd
    BLAST
    Regioni539 – 7892516-phosphogluconolactonaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0363.
    GeneTreeiENSGT00530000063435.
    HOGENOMiHOG000231077.
    HOVERGENiHBG005780.
    InParanoidiB2KGW7.
    KOiK13937.
    OrthoDBiEOG7BW0HS.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR005900. 6-phosphogluconolactonase_DevB.
    IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR006148. Glc/Gal-6P_isomerase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    PF01182. Glucosamine_iso. 1 hit.
    [Graphical view]
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR01198. pgl. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CFX1-1 [UniParc]FASTAAdd to Basket

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    MLLAAMCLAL LGCLQAQELK GHVSIILLGA TGDLAKKYLW QGLFQLYLDE    50
    AGKGHSFSFH GAALTAPQQG QKLMDKVLES LSCPKDLVPS RCDELKGQFL 100
    QLSQYRQLKT VEDYQTLNKD IETQVQQDGL WEAGRIFYFS VPPFAYADIA 150
    RNINSSCRPH PGAWLRVVFE KPFGHDHLSA QQLASELGSF FQEEEMYRVD 200
    HYLGKQAVAQ ILPFRDQNRK ALDGLWNRHH VERVEIILKE TIDAEGRASF 250
    YEEYGVIRDT LQNHLTEILT LVAMELPLNI SSSAAVLQHK LWAFQALRGL 300
    QKSSAILGQY QAYSGQVRRE LQKPDGFQSL TPTFAGVLVH IDNLRWEGVP 350
    FILMSGKALD ERVGYVRIVF KNRAYCTQSE RHWVPEQSRC LPQQIIFYIG 400
    HGELGHPAIL VSRNLFKPSL PTQKWKEVQD QPGLRLFGRP LSDYYAYRPV 450
    REQDAYSTLL SHIFHCRKES FITTENLLAS WVFWTPLLDS LAFEVPRPYP 500
    GGAENGQLLD FEFSGGQLTF SQQQLEVLIP DLGSVPKPSD FQVLGARYRQ 550
    SPLITAWPEE LISKLASDIE AAAVQAVRHF GKFHLALSGG SSPIALFQQL 600
    ATGHYSFPWA HTHLWLVDER CVPLSDPDSN FQGLQAHLLQ HVRVPYYNIH 650
    PMPVHLHQRL CAEEDQGAQT YASEISALVA NSSFDLVLLG MGTDGHTASL 700
    FPQSPTGLDG DQLVVLTESP FRPHQRMSLS LPLINRAKKV AVLVMGRTKR 750
    EITTLVSRVG HEPKKWPISG VVPLSGQLVW YMDYEAFLG 789
    Length:789
    Mass (Da):88,928
    Last modified:July 27, 2011 - v2
    Checksum:iE970CE8B35D3E1E5
    GO

    Sequence cautioni

    The sequence BAC32260.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771V → A in BAE35029. (PubMed:16141072)Curated
    Sequence conflicti77 – 771V → A in CAQ51682. (PubMed:19468303)Curated
    Sequence conflicti77 – 771V → A in AAH42677. (PubMed:15489334)Curated
    Sequence conflicti106 – 1061R → H in BAE35029. (PubMed:16141072)Curated
    Sequence conflicti106 – 1061R → H in CAQ51682. (PubMed:19468303)Curated
    Sequence conflicti106 – 1061R → H in AAH42677. (PubMed:15489334)Curated
    Sequence conflicti285 – 2851A → T in BAE35029. (PubMed:16141072)Curated
    Sequence conflicti285 – 2851A → T in CAQ51682. (PubMed:19468303)Curated
    Sequence conflicti285 – 2851A → T in AAH42677. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK045199 mRNA. Translation: BAC32260.1. Different initiation.
    AK159373 mRNA. Translation: BAE35029.1.
    AL606914 Genomic DNA. Translation: CAM16119.1.
    CU463327 Genomic DNA. Translation: CAQ51682.1.
    BC042677 mRNA. Translation: AAH42677.1.
    CCDSiCCDS71524.1.
    RefSeqiNP_001277933.1. NM_001291004.1.
    NP_775547.2. NM_173371.4.
    XP_006537124.1. XM_006537061.1.
    XP_006538524.1. XM_006538461.1.
    UniGeneiMm.22183.

    Genome annotation databases

    EnsembliENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980.
    GeneIDi100198.
    KEGGimmu:100198.
    UCSCiuc008vxh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK045199 mRNA. Translation: BAC32260.1 . Different initiation.
    AK159373 mRNA. Translation: BAE35029.1 .
    AL606914 Genomic DNA. Translation: CAM16119.1 .
    CU463327 Genomic DNA. Translation: CAQ51682.1 .
    BC042677 mRNA. Translation: AAH42677.1 .
    CCDSi CCDS71524.1.
    RefSeqi NP_001277933.1. NM_001291004.1.
    NP_775547.2. NM_173371.4.
    XP_006537124.1. XM_006537061.1.
    XP_006538524.1. XM_006538461.1.
    UniGenei Mm.22183.

    3D structure databases

    ProteinModelPortali Q8CFX1.
    SMRi Q8CFX1. Positions 25-500, 554-786.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8CFX1. 3 interactions.
    MINTi MINT-1837869.

    PTM databases

    PhosphoSitei Q8CFX1.

    Proteomic databases

    MaxQBi Q8CFX1.
    PaxDbi Q8CFX1.
    PRIDEi Q8CFX1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000084117 ; ENSMUSP00000081134 ; ENSMUSG00000028980 .
    GeneIDi 100198.
    KEGGi mmu:100198.
    UCSCi uc008vxh.1. mouse.

    Organism-specific databases

    CTDi 9563.
    MGIi MGI:2140356. H6pd.

    Phylogenomic databases

    eggNOGi COG0363.
    GeneTreei ENSGT00530000063435.
    HOGENOMi HOG000231077.
    HOVERGENi HBG005780.
    InParanoidi B2KGW7.
    KOi K13937.
    OrthoDBi EOG7BW0HS.

    Enzyme and pathway databases

    SABIO-RK Q8CFX1.

    Miscellaneous databases

    NextBioi 354313.
    PROi Q8CFX1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CFX1.
    Bgeei Q8CFX1.
    Genevestigatori Q8CFX1.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR005900. 6-phosphogluconolactonase_DevB.
    IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR006148. Glc/Gal-6P_isomerase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    PF01182. Glucosamine_iso. 1 hit.
    [Graphical view ]
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR01198. pgl. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-205 AND LYS-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiG6PE_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFX1
    Secondary accession number(s): A2A7A9, B2KGW7, Q8BLH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3