Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GDH/6PGL endoplasmic bifunctional protein

Gene

H6pd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.By similarity

Catalytic activityi

D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.PROSITE-ProRule annotation
D-glucose 6-phosphate + NAD(P)+ = D-glucono-1,5-lactone 6-phosphate + NAD(P)H.
6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31NADPBy similarity1
Binding sitei63NADPBy similarity1
Binding sitei201SubstratePROSITE-ProRule annotation1
Binding sitei205SubstratePROSITE-ProRule annotation1
Active sitei264Proton acceptorBy similarity1

GO - Molecular functioni

  • 6-phosphogluconolactonase activity Source: MGI
  • glucose 1-dehydrogenase [NAD(P)] activity Source: UniProtKB-EC
  • glucose-6-phosphate dehydrogenase activity Source: MGI
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: UniProtKB-KW
  • pentose-phosphate shunt Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

SABIO-RKQ8CFX1.

Names & Taxonomyi

Protein namesi
Recommended name:
GDH/6PGL endoplasmic bifunctional protein
Including the following 2 domains:
Glucose 1-dehydrogenase (EC:1.1.1.47)
Alternative name(s):
Hexose-6-phosphate dehydrogenase
6-phosphogluconolactonase (EC:3.1.1.31)
Short name:
6PGL
Gene namesi
Name:H6pd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2140356. H6pd.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000023679417 – 789GDH/6PGL endoplasmic bifunctional proteinAdd BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17Pyrrolidone carboxylic acidBy similarity1
Glycosylationi154N-linked (GlcNAc...)Sequence analysis1
Modified residuei205N6-succinyllysineCombined sources1
Glycosylationi279N-linked (GlcNAc...)Sequence analysis1
Modified residuei424N6-succinyllysineCombined sources1
Glycosylationi681N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

EPDiQ8CFX1.
MaxQBiQ8CFX1.
PaxDbiQ8CFX1.
PRIDEiQ8CFX1.

PTM databases

iPTMnetiQ8CFX1.
PhosphoSitePlusiQ8CFX1.
SwissPalmiQ8CFX1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028980.
ExpressionAtlasiQ8CFX1. baseline and differential.
GenevisibleiQ8CFX1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8CFX1. 3 interactors.
MINTiMINT-1837869.
STRINGi10090.ENSMUSP00000030830.

Structurei

3D structure databases

ProteinModelPortaliQ8CFX1.
SMRiQ8CFX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 524Glucose 1-dehydrogenaseAdd BLAST508
Regioni525 – 538LinkerAdd BLAST14
Regioni539 – 7896-phosphogluconolactonaseAdd BLAST251

Sequence similaritiesi

In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
KOG3147. Eukaryota.
COG0363. LUCA.
COG0364. LUCA.
GeneTreeiENSGT00530000063435.
HOGENOMiHOG000231077.
HOVERGENiHBG005780.
InParanoidiQ8CFX1.
KOiK13937.

Family and domain databases

CDDicd01400. 6PGL. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR005900. 6-phosphogluconolactonase_DevB.
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR006148. Glc/Gal-6P_isomerase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PF01182. Glucosamine_iso. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01198. pgl. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLAAMCLAL LGCLQAQELK GHVSIILLGA TGDLAKKYLW QGLFQLYLDE
60 70 80 90 100
AGKGHSFSFH GAALTAPQQG QKLMDKVLES LSCPKDLVPS RCDELKGQFL
110 120 130 140 150
QLSQYRQLKT VEDYQTLNKD IETQVQQDGL WEAGRIFYFS VPPFAYADIA
160 170 180 190 200
RNINSSCRPH PGAWLRVVFE KPFGHDHLSA QQLASELGSF FQEEEMYRVD
210 220 230 240 250
HYLGKQAVAQ ILPFRDQNRK ALDGLWNRHH VERVEIILKE TIDAEGRASF
260 270 280 290 300
YEEYGVIRDT LQNHLTEILT LVAMELPLNI SSSAAVLQHK LWAFQALRGL
310 320 330 340 350
QKSSAILGQY QAYSGQVRRE LQKPDGFQSL TPTFAGVLVH IDNLRWEGVP
360 370 380 390 400
FILMSGKALD ERVGYVRIVF KNRAYCTQSE RHWVPEQSRC LPQQIIFYIG
410 420 430 440 450
HGELGHPAIL VSRNLFKPSL PTQKWKEVQD QPGLRLFGRP LSDYYAYRPV
460 470 480 490 500
REQDAYSTLL SHIFHCRKES FITTENLLAS WVFWTPLLDS LAFEVPRPYP
510 520 530 540 550
GGAENGQLLD FEFSGGQLTF SQQQLEVLIP DLGSVPKPSD FQVLGARYRQ
560 570 580 590 600
SPLITAWPEE LISKLASDIE AAAVQAVRHF GKFHLALSGG SSPIALFQQL
610 620 630 640 650
ATGHYSFPWA HTHLWLVDER CVPLSDPDSN FQGLQAHLLQ HVRVPYYNIH
660 670 680 690 700
PMPVHLHQRL CAEEDQGAQT YASEISALVA NSSFDLVLLG MGTDGHTASL
710 720 730 740 750
FPQSPTGLDG DQLVVLTESP FRPHQRMSLS LPLINRAKKV AVLVMGRTKR
760 770 780
EITTLVSRVG HEPKKWPISG VVPLSGQLVW YMDYEAFLG
Length:789
Mass (Da):88,928
Last modified:July 27, 2011 - v2
Checksum:iE970CE8B35D3E1E5
GO

Sequence cautioni

The sequence BAC32260 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77V → A in BAE35029 (PubMed:16141072).Curated1
Sequence conflicti77V → A in CAQ51682 (PubMed:19468303).Curated1
Sequence conflicti77V → A in AAH42677 (PubMed:15489334).Curated1
Sequence conflicti106R → H in BAE35029 (PubMed:16141072).Curated1
Sequence conflicti106R → H in CAQ51682 (PubMed:19468303).Curated1
Sequence conflicti106R → H in AAH42677 (PubMed:15489334).Curated1
Sequence conflicti285A → T in BAE35029 (PubMed:16141072).Curated1
Sequence conflicti285A → T in CAQ51682 (PubMed:19468303).Curated1
Sequence conflicti285A → T in AAH42677 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045199 mRNA. Translation: BAC32260.1. Different initiation.
AK159373 mRNA. Translation: BAE35029.1.
AL606914 Genomic DNA. Translation: CAM16119.1.
CU463327 Genomic DNA. Translation: CAQ51682.1.
BC042677 mRNA. Translation: AAH42677.1.
CCDSiCCDS71524.1.
RefSeqiNP_001277933.1. NM_001291004.1.
NP_775547.2. NM_173371.4.
UniGeneiMm.22183.

Genome annotation databases

EnsembliENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980.
GeneIDi100198.
KEGGimmu:100198.
UCSCiuc008vxh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045199 mRNA. Translation: BAC32260.1. Different initiation.
AK159373 mRNA. Translation: BAE35029.1.
AL606914 Genomic DNA. Translation: CAM16119.1.
CU463327 Genomic DNA. Translation: CAQ51682.1.
BC042677 mRNA. Translation: AAH42677.1.
CCDSiCCDS71524.1.
RefSeqiNP_001277933.1. NM_001291004.1.
NP_775547.2. NM_173371.4.
UniGeneiMm.22183.

3D structure databases

ProteinModelPortaliQ8CFX1.
SMRiQ8CFX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CFX1. 3 interactors.
MINTiMINT-1837869.
STRINGi10090.ENSMUSP00000030830.

PTM databases

iPTMnetiQ8CFX1.
PhosphoSitePlusiQ8CFX1.
SwissPalmiQ8CFX1.

Proteomic databases

EPDiQ8CFX1.
MaxQBiQ8CFX1.
PaxDbiQ8CFX1.
PRIDEiQ8CFX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980.
GeneIDi100198.
KEGGimmu:100198.
UCSCiuc008vxh.2. mouse.

Organism-specific databases

CTDi9563.
MGIiMGI:2140356. H6pd.

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
KOG3147. Eukaryota.
COG0363. LUCA.
COG0364. LUCA.
GeneTreeiENSGT00530000063435.
HOGENOMiHOG000231077.
HOVERGENiHBG005780.
InParanoidiQ8CFX1.
KOiK13937.

Enzyme and pathway databases

SABIO-RKQ8CFX1.

Miscellaneous databases

PROiQ8CFX1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028980.
ExpressionAtlasiQ8CFX1. baseline and differential.
GenevisibleiQ8CFX1. MM.

Family and domain databases

CDDicd01400. 6PGL. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR005900. 6-phosphogluconolactonase_DevB.
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR006148. Glc/Gal-6P_isomerase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PF01182. Glucosamine_iso. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01198. pgl. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PE_MOUSE
AccessioniPrimary (citable) accession number: Q8CFX1
Secondary accession number(s): A2A7A9, B2KGW7, Q8BLH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.