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Q8CFX1 (G6PE_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GDH/6PGL endoplasmic bifunctional protein

Including the following 2 domains:

  1. Glucose 1-dehydrogenase
    EC=1.1.1.47
    Alternative name(s):
    Hexose-6-phosphate dehydrogenase
  2. 6-phosphogluconolactonase
    Short name=6PGL
    EC=3.1.1.31
Gene names
Name:H6pd
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length789 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates By similarity.

Catalytic activity

Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.

D-glucose 6-phosphate + NAD(P)+ = D-glucono-1,5-lactone 6-phosphate + NAD(P)H.

6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

Subcellular location

Endoplasmic reticulum lumen By similarity. Note: Microsomes, endoplasmic reticulum lumen By similarity.

Sequence similarities

In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.

In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.

Sequence caution

The sequence BAC32260.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 789773GDH/6PGL endoplasmic bifunctional protein
PRO_0000236794

Regions

Region17 – 524508Glucose 1-dehydrogenase
Region525 – 53814Linker
Region539 – 7892516-phosphogluconolactonase

Sites

Active site2641Proton acceptor By similarity
Binding site311NADP By similarity
Binding site631NADP By similarity
Binding site2011Substrate By similarity
Binding site2051Substrate By similarity

Amino acid modifications

Modified residue171Pyrrolidone carboxylic acid By similarity
Modified residue2051N6-succinyllysine Ref.4
Modified residue4241N6-succinyllysine Ref.4
Glycosylation1541N-linked (GlcNAc...) Potential
Glycosylation2791N-linked (GlcNAc...) Potential
Glycosylation6811N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict771V → A in BAE35029. Ref.1
Sequence conflict771V → A in CAQ51682. Ref.2
Sequence conflict771V → A in AAH42677. Ref.3
Sequence conflict1061R → H in BAE35029. Ref.1
Sequence conflict1061R → H in CAQ51682. Ref.2
Sequence conflict1061R → H in AAH42677. Ref.3
Sequence conflict2851A → T in BAE35029. Ref.1
Sequence conflict2851A → T in CAQ51682. Ref.2
Sequence conflict2851A → T in AAH42677. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8CFX1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: E970CE8B35D3E1E5

FASTA78988,928
        10         20         30         40         50         60 
MLLAAMCLAL LGCLQAQELK GHVSIILLGA TGDLAKKYLW QGLFQLYLDE AGKGHSFSFH 

        70         80         90        100        110        120 
GAALTAPQQG QKLMDKVLES LSCPKDLVPS RCDELKGQFL QLSQYRQLKT VEDYQTLNKD 

       130        140        150        160        170        180 
IETQVQQDGL WEAGRIFYFS VPPFAYADIA RNINSSCRPH PGAWLRVVFE KPFGHDHLSA 

       190        200        210        220        230        240 
QQLASELGSF FQEEEMYRVD HYLGKQAVAQ ILPFRDQNRK ALDGLWNRHH VERVEIILKE 

       250        260        270        280        290        300 
TIDAEGRASF YEEYGVIRDT LQNHLTEILT LVAMELPLNI SSSAAVLQHK LWAFQALRGL 

       310        320        330        340        350        360 
QKSSAILGQY QAYSGQVRRE LQKPDGFQSL TPTFAGVLVH IDNLRWEGVP FILMSGKALD 

       370        380        390        400        410        420 
ERVGYVRIVF KNRAYCTQSE RHWVPEQSRC LPQQIIFYIG HGELGHPAIL VSRNLFKPSL 

       430        440        450        460        470        480 
PTQKWKEVQD QPGLRLFGRP LSDYYAYRPV REQDAYSTLL SHIFHCRKES FITTENLLAS 

       490        500        510        520        530        540 
WVFWTPLLDS LAFEVPRPYP GGAENGQLLD FEFSGGQLTF SQQQLEVLIP DLGSVPKPSD 

       550        560        570        580        590        600 
FQVLGARYRQ SPLITAWPEE LISKLASDIE AAAVQAVRHF GKFHLALSGG SSPIALFQQL 

       610        620        630        640        650        660 
ATGHYSFPWA HTHLWLVDER CVPLSDPDSN FQGLQAHLLQ HVRVPYYNIH PMPVHLHQRL 

       670        680        690        700        710        720 
CAEEDQGAQT YASEISALVA NSSFDLVLLG MGTDGHTASL FPQSPTGLDG DQLVVLTESP 

       730        740        750        760        770        780 
FRPHQRMSLS LPLINRAKKV AVLVMGRTKR EITTLVSRVG HEPKKWPISG VVPLSGQLVW 


YMDYEAFLG 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[4]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-205 AND LYS-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK045199 mRNA. Translation: BAC32260.1. Different initiation.
AK159373 mRNA. Translation: BAE35029.1.
AL606914 Genomic DNA. Translation: CAM16119.1.
CU463327 Genomic DNA. Translation: CAQ51682.1.
BC042677 mRNA. Translation: AAH42677.1.
RefSeqNP_001277933.1. NM_001291004.1.
NP_775547.2. NM_173371.4.
XP_006537124.1. XM_006537061.1.
XP_006538524.1. XM_006538461.1.
UniGeneMm.22183.

3D structure databases

ProteinModelPortalQ8CFX1.
SMRQ8CFX1. Positions 25-500, 554-786.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8CFX1. 3 interactions.
MINTMINT-1837869.

PTM databases

PhosphoSiteQ8CFX1.

Proteomic databases

MaxQBQ8CFX1.
PaxDbQ8CFX1.
PRIDEQ8CFX1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980.
GeneID100198.
KEGGmmu:100198.
UCSCuc008vxh.1. mouse.

Organism-specific databases

CTD9563.
MGIMGI:2140356. H6pd.

Phylogenomic databases

eggNOGCOG0363.
GeneTreeENSGT00530000063435.
HOGENOMHOG000231077.
HOVERGENHBG005780.
InParanoidB2KGW7.
KOK13937.
OrthoDBEOG7BW0HS.

Enzyme and pathway databases

SABIO-RKQ8CFX1.

Gene expression databases

ArrayExpressQ8CFX1.
BgeeQ8CFX1.
GenevestigatorQ8CFX1.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR005900. 6-phosphogluconolactonase_DevB.
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR006148. Glc/Gal-6P_isomerase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PF01182. Glucosamine_iso. 1 hit.
[Graphical view]
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR01198. pgl. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio354313.
PROQ8CFX1.
SOURCESearch...

Entry information

Entry nameG6PE_MOUSE
AccessionPrimary (citable) accession number: Q8CFX1
Secondary accession number(s): A2A7A9, B2KGW7, Q8BLH1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot