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Protein

Anoctamin-2

Gene

Ano2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.5 Publications

Enzyme regulationi

Channel activity is repressed by chloride inhibitors; strongly by niflumic acid (NFA), partially by flufenamic acid (FFA), and only slightly by meclofenamic acid (MFA), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS), and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS).1 Publication

GO - Molecular functioni

  • intracellular calcium activated chloride channel activity Source: UniProtKB
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • chloride transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-2By similarity
Alternative name(s):
Transmembrane protein 16BImported
Gene namesi
Name:Ano2By similarity
Synonyms:Tmem16bImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2387214. Ano2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 364CytoplasmicSequence analysisAdd BLAST364
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Topological domaini386 – 433ExtracellularSequence analysisAdd BLAST48
Transmembranei434 – 454HelicalSequence analysisAdd BLAST21
Topological domaini455 – 536CytoplasmicSequence analysisAdd BLAST82
Transmembranei537 – 557HelicalSequence analysisAdd BLAST21
Topological domaini558 – 580ExtracellularSequence analysisAdd BLAST23
Transmembranei581 – 601HelicalSequence analysisAdd BLAST21
Topological domaini602 – 621CytoplasmicSequence analysisAdd BLAST20
Transmembranei622 – 642HelicalSequence analysisAdd BLAST21
Topological domaini643 – 746ExtracellularSequence analysisAdd BLAST104
Transmembranei747 – 767HelicalSequence analysisAdd BLAST21
Topological domaini768 – 799CytoplasmicSequence analysisAdd BLAST32
Transmembranei800 – 820HelicalSequence analysisAdd BLAST21
Topological domaini821 – 905ExtracellularSequence analysisAdd BLAST85
Transmembranei906 – 926HelicalSequence analysisAdd BLAST21
Topological domaini927 – 1002CytoplasmicSequence analysisAdd BLAST76

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • non-motile cilium Source: MGI
  • nucleoplasm Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1000 – 1002Missing : Impaired interaction with DLG4. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003531871 – 1002Anoctamin-2Add BLAST1002

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi421N-linked (GlcNAc...)Sequence analysis1
Glycosylationi839N-linked (GlcNAc...)Sequence analysis1
Glycosylationi847N-linked (GlcNAc...)Sequence analysis1
Glycosylationi854N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8CFW1.
PRIDEiQ8CFW1.

PTM databases

iPTMnetiQ8CFW1.
PhosphoSitePlusiQ8CFW1.

Expressioni

Tissue specificityi

Expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia (at protein level). Also observed in retinal pigment epithelium (RPE), olfactory bulb, brain, and cortex.3 Publications

Developmental stagei

Detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. In developing skin, expression is restricted to basal layers of the epidermis at E16.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000038115.
ExpressionAtlasiQ8CFW1. baseline and differential.

Interactioni

Subunit structurei

Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminal region.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125303.

Structurei

3D structure databases

ProteinModelPortaliQ8CFW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1000 – 1002DLG4 binding (PDZ)3

Sequence similaritiesi

Belongs to the anoctamin family.By similarity

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2514. Eukaryota.
ENOG410XS4S. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ8CFW1.
KOiK19497.
OMAiDEDKLTW.
OrthoDBiEOG091G01JF.
TreeFamiTF314265.

Family and domain databases

InterProiIPR032394. Anoct_dimer.
IPR007632. Anoctamin.
IPR031288. Anoctamin-2.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 2 hits.
PTHR12308:SF20. PTHR12308:SF20. 2 hits.
PfamiPF16178. Anoct_dimer. 1 hit.
PF04547. Anoctamin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CFW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGLRDIP LLPGSPRRLS SRTVARGSQG PKHGQQYLKV PGHRAPGQRD
60 70 80 90 100
NSSLHPSQVS RRESSRDRSV INNYLDANEP PSSEARLSRM HFHDNQRKVD
110 120 130 140 150
YVLAYHYRKR GAHLGHGSPG HSLAVISNGE TGKERHGGGP GDVELGPLDA
160 170 180 190 200
LEEERREQRD EFEHNLMAAG LELEKDLESK SQGSVFVRIH APWQVLAREA
210 220 230 240 250
EFLKIKVPTK KMYEIKAGGS IAKKFSAILQ TLSSPLQPRV PEHSNNRMKN
260 270 280 290 300
LSYPFSREKM YLYNIQEKDT FFDNATRSRI VHEILKRTAC SRANNTMGIN
310 320 330 340 350
SLIANNIYEA AYPLHDGEYD SPGDDMNDRK LLYQEWARYG VFYKFQPIDL
360 370 380 390 400
IRKYFGEKIG LYFAWLGLYT SFLIPSSVIG VIVFLYGCAT IEEDIPSKEM
410 420 430 440 450
CDHQNAFTMC PLCDKSCDYW NLSSACGTAR ASHLFDNPAT VFFSIFMALW
460 470 480 490 500
ATMFLENWKR LQMRLGYFWD LTGIEEEEER SQEHSRPEYE TKVREKLLKE
510 520 530 540 550
SGKSAVQKLE ANSPEDDEDD EDKLTWKDRF PGYLMNFASI LFMIALTFSI
560 570 580 590 600
VFGVIVYRIT TAAALSLNKA TRSNVRVTVT ATAVIINLVV ILILDEIYGA
610 620 630 640 650
VAKWLTKIEV PKTEQTFEER LILKAFLLKF VNAYSPIFYV AFFKGRFVGR
660 670 680 690 700
PGSYVYVFDG YRMEECAPGG CLMELCIQLS IIMLGKQLIQ NNIFEIGVPK
710 720 730 740 750
LKKLFRKLKD ETEPGESDPD HSKRPEQWDL DHSLEPYTGL TPEYMEMIIQ
760 770 780 790 800
FGFVTLFVAS FPLAPVFALL NNVIEVRLDA KKFVTELRRP DAVRTKDIGI
810 820 830 840 850
WFDILSGIGK FSVIINAFVI AVTSDFIPRL VYQYSYSHNG TLHGFVNHTL
860 870 880 890 900
SFFNVSQLKE GTQPENSQFD QEVQFCRFKD YREPPWAPNP YEFSKQYWSV
910 920 930 940 950
LSARLAFVII FQNLVMFLSV LVDWMIPDIP TDISDQIKKE KSLLVDFFLK
960 970 980 990 1000
EEHEKVKLAD EPTQRSQGGG DRSRRSRAAS SAPSGRSQPG SIASSGSQHT

NV
Length:1,002
Mass (Da):114,134
Last modified:November 3, 2009 - v2
Checksum:i8514FC9CC13F78C7
GO

Sequence cautioni

The sequence AAH33409 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC32073 differs from that shown. Probable cloning artifact.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ384096 mRNA. Translation: ACL36051.1.
AK044763 mRNA. Translation: BAC32073.1. Sequence problems.
BC033409 mRNA. Translation: AAH33409.1. Different initiation.
CCDSiCCDS39643.2.
RefSeqiNP_705817.2. NM_153589.2.
XP_006506182.1. XM_006506119.1.
UniGeneiMm.333398.

Genome annotation databases

EnsembliENSMUST00000160496; ENSMUSP00000125303; ENSMUSG00000038115.
GeneIDi243634.
KEGGimmu:243634.
UCSCiuc009duv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ384096 mRNA. Translation: ACL36051.1.
AK044763 mRNA. Translation: BAC32073.1. Sequence problems.
BC033409 mRNA. Translation: AAH33409.1. Different initiation.
CCDSiCCDS39643.2.
RefSeqiNP_705817.2. NM_153589.2.
XP_006506182.1. XM_006506119.1.
UniGeneiMm.333398.

3D structure databases

ProteinModelPortaliQ8CFW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125303.

PTM databases

iPTMnetiQ8CFW1.
PhosphoSitePlusiQ8CFW1.

Proteomic databases

PaxDbiQ8CFW1.
PRIDEiQ8CFW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160496; ENSMUSP00000125303; ENSMUSG00000038115.
GeneIDi243634.
KEGGimmu:243634.
UCSCiuc009duv.1. mouse.

Organism-specific databases

CTDi57101.
MGIiMGI:2387214. Ano2.

Phylogenomic databases

eggNOGiKOG2514. Eukaryota.
ENOG410XS4S. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ8CFW1.
KOiK19497.
OMAiDEDKLTW.
OrthoDBiEOG091G01JF.
TreeFamiTF314265.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

ChiTaRSiAno2. mouse.
PROiQ8CFW1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038115.
ExpressionAtlasiQ8CFW1. baseline and differential.

Family and domain databases

InterProiIPR032394. Anoct_dimer.
IPR007632. Anoctamin.
IPR031288. Anoctamin-2.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 2 hits.
PTHR12308:SF20. PTHR12308:SF20. 2 hits.
PfamiPF16178. Anoct_dimer. 1 hit.
PF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANO2_MOUSE
AccessioniPrimary (citable) accession number: Q8CFW1
Secondary accession number(s): C4N788, Q8C8N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: November 3, 2009
Last modified: November 30, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.