Anoctamin-2 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Anoctamin-2

Alternative name(s):
Transmembrane protein 16B

Gene names

Name:	Ano2
Synonyms:	Tmem16b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1002 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina. Ref.1 Ref.5 Ref.6 Ref.8 Ref.10
Enzyme regulation	Channel activity is repressed by chloride inhibitors; strongly by niflumic acid (NFA), partially by flufenamic acid (FFA), and only slightly by meclofenamic acid (MFA), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS), and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Ref.5
Subunit structure	Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminus region. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.1 Ref.6.
Tissue specificity	Expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia (at protein level). Also observed in retinal pigment epithelium (RPE), olfactory bulb, brain, and cortex. Ref.1 Ref.6 Ref.7
Developmental stage	Detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. In developing skin, expression is restricted to basal layers of the epidermis at E16.5. Ref.4
Post-translational modification	Glycosylated. Ref.1
Miscellaneous	The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.
Sequence similarities	Belongs to the anoctamin family. UniProtKB Q9NQ90
Sequence caution	The sequence AAH33409.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC32073.1 differs from that shown. Reason: Probable cloning artifact.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Calcium Chloride
Molecular function	Chloride channel Ion channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	intracellular calcium activated chloride channel activity Inferred from direct assay Ref.1 Ref.5 Ref.6 Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1002

1002

Anoctamin-2

PRO_0000353187

Regions

Topological domain

1 – 364

364

Cytoplasmic Potential

Transmembrane

365 – 385

21

Helical; Potential

Topological domain

386 – 433

48

Extracellular Potential

Transmembrane

434 – 454

21

Helical; Potential

Topological domain

455 – 536

82

Cytoplasmic Potential

Transmembrane

537 – 557

21

Helical; Potential

Topological domain

558 – 580

23

Extracellular Potential

Transmembrane

581 – 601

21

Helical; Potential

Topological domain

602 – 621

20

Cytoplasmic Potential

Transmembrane

622 – 642

21

Helical; Potential

Topological domain

643 – 746

104

Extracellular Potential

Transmembrane

747 – 767

21

Helical; Potential

Topological domain

768 – 799

32

Cytoplasmic Potential

Transmembrane

800 – 820

21

Helical; Potential

Topological domain

821 – 905

85

Extracellular Potential

Transmembrane

906 – 926

21

Helical; Potential

Topological domain

927 – 1002

76

Cytoplasmic Potential

Motif

1000 – 1002

3

DLG4 binding (PDZ)

Amino acid modifications

Glycosylation

421

1

N-linked (GlcNAc...) Potential

Glycosylation

839

1

N-linked (GlcNAc...) Potential

Glycosylation

847

1

N-linked (GlcNAc...) Potential

Glycosylation

854

1

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

1000 – 1002

3

Missing: Impaired interaction with DLG4. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CFW1 [UniParc].

Last modified November 3, 2009. Version 2.
Checksum: 8514FC9CC13F78C7
Show »

FASTA

1,002

114,134

        10         20         30         40         50         60 
MAAPGLRDIP LLPGSPRRLS SRTVARGSQG PKHGQQYLKV PGHRAPGQRD NSSLHPSQVS 

        70         80         90        100        110        120 
RRESSRDRSV INNYLDANEP PSSEARLSRM HFHDNQRKVD YVLAYHYRKR GAHLGHGSPG 

       130        140        150        160        170        180 
HSLAVISNGE TGKERHGGGP GDVELGPLDA LEEERREQRD EFEHNLMAAG LELEKDLESK 

       190        200        210        220        230        240 
SQGSVFVRIH APWQVLAREA EFLKIKVPTK KMYEIKAGGS IAKKFSAILQ TLSSPLQPRV 

       250        260        270        280        290        300 
PEHSNNRMKN LSYPFSREKM YLYNIQEKDT FFDNATRSRI VHEILKRTAC SRANNTMGIN 

       310        320        330        340        350        360 
SLIANNIYEA AYPLHDGEYD SPGDDMNDRK LLYQEWARYG VFYKFQPIDL IRKYFGEKIG 

       370        380        390        400        410        420 
LYFAWLGLYT SFLIPSSVIG VIVFLYGCAT IEEDIPSKEM CDHQNAFTMC PLCDKSCDYW 

       430        440        450        460        470        480 
NLSSACGTAR ASHLFDNPAT VFFSIFMALW ATMFLENWKR LQMRLGYFWD LTGIEEEEER 

       490        500        510        520        530        540 
SQEHSRPEYE TKVREKLLKE SGKSAVQKLE ANSPEDDEDD EDKLTWKDRF PGYLMNFASI 

       550        560        570        580        590        600 
LFMIALTFSI VFGVIVYRIT TAAALSLNKA TRSNVRVTVT ATAVIINLVV ILILDEIYGA 

       610        620        630        640        650        660 
VAKWLTKIEV PKTEQTFEER LILKAFLLKF VNAYSPIFYV AFFKGRFVGR PGSYVYVFDG 

       670        680        690        700        710        720 
YRMEECAPGG CLMELCIQLS IIMLGKQLIQ NNIFEIGVPK LKKLFRKLKD ETEPGESDPD 

       730        740        750        760        770        780 
HSKRPEQWDL DHSLEPYTGL TPEYMEMIIQ FGFVTLFVAS FPLAPVFALL NNVIEVRLDA 

       790        800        810        820        830        840 
KKFVTELRRP DAVRTKDIGI WFDILSGIGK FSVIINAFVI AVTSDFIPRL VYQYSYSHNG 

       850        860        870        880        890        900 
TLHGFVNHTL SFFNVSQLKE GTQPENSQFD QEVQFCRFKD YREPPWAPNP YEFSKQYWSV 

       910        920        930        940        950        960 
LSARLAFVII FQNLVMFLSV LVDWMIPDIP TDISDQIKKE KSLLVDFFLK EEHEKVKLAD 

       970        980        990       1000 
EPTQRSQGGG DRSRRSRAAS SAPSGRSQPG SIASSGSQHT NV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals." Stoehr H., Heisig J.B., Benz P.M., Schoeberl S., Milenkovic V.M., Strauss O., Aartsen W.M., Wijnholds J., Weber B.H.F., Schulz H.L. J. Neurosci. 29:6809-6818(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH DLG4, MUTAGENESIS OF 1000-THR--ASN-1002, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Retina.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-543. Strain: C57BL/6J. Tissue: Retina.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-1002. Strain: C57BL/6. Tissue: Eye.
[4]	"Expression of TMEM16 paralogs during murine embryogenesis." Rock J.R., Harfe B.D. Dev. Dyn. 237:2566-2574(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[5]	"TMEM16B induces chloride currents activated by calcium in mammalian cells." Pifferi S., Dibattista M., Menini A. Pflugers Arch. 458:1023-1038(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME REGULATION.
[6]	"ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification." Stephan A.B., Shum E.Y., Hirsh S., Cygnar K.D., Reisert J., Zhao H. Proc. Natl. Acad. Sci. U.S.A. 106:11776-11781(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Expression and function of epithelial anoctamins." Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M., Martins J.R., Kunzelmann K. J. Biol. Chem. 285:7838-7845(2010) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins." Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C. Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age." Winpenny J.P., Gray M.A. Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[10]	"Anoctamin 2/TMEM16B: a calcium-activated chloride channel in olfactory transduction." Pifferi S., Cenedese V., Menini A. Exp. Physiol. 97:193-199(2012) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FJ384096 mRNA. Translation: ACL36051.1. AK044763 mRNA. Translation: BAC32073.1. Sequence problems. BC033409 mRNA. Translation: AAH33409.1. Different initiation.
IPI	IPI00405978.
RefSeq	NP_705817.2. NM_153589.2.
UniGene	Mm.333398.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8CFW1.
Proteomic databases
PRIDE	Q8CFW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000160496; ENSMUSP00000125303; ENSMUSG00000038115.
GeneID	243634.
KEGG	mmu:243634.
UCSC	uc009duv.1. mouse.
Organism-specific databases
CTD	57101.
MGI	MGI:2387214. Ano2.
Phylogenomic databases
eggNOG	NOG320103.
GeneTree	ENSGT00700000104119.
HOGENOM	HOG000006509.
HOVERGEN	HBG069519.
InParanoid	Q8CFW1.
OMA	VPTKKEM.
OrthoDB	EOG470TGS.
Gene expression databases
ArrayExpress	Q8CFW1.
Bgee	Q8CFW1.
Genevestigator	Q8CFW1.
Family and domain databases
InterPro	IPR007632. Anoctamin. [Graphical view]
PANTHER	PTHR12308. PTHR12308. 1 hit.
Pfam	PF04547. Anoctamin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	385867.
SOURCE	Search...

Entry information

Entry name

ANO2_MOUSE

Accession

Primary (citable) accession number: Q8CFW1
Secondary accession number(s): C4N788, Q8C8N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	November 3, 2009
Last modified:	April 3, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents