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Q8CFW1

- ANO2_MOUSE

UniProt

Q8CFW1 - ANO2_MOUSE

Protein

Anoctamin-2

Gene

Ano2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (03 Nov 2009)
      Previous versions | rss
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    Functioni

    Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.5 Publications

    Enzyme regulationi

    Channel activity is repressed by chloride inhibitors; strongly by niflumic acid (NFA), partially by flufenamic acid (FFA), and only slightly by meclofenamic acid (MFA), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS), and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS).1 Publication

    GO - Molecular functioni

    1. intracellular calcium activated chloride channel activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. protein heterodimerization activity Source: MGI
    4. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. chloride transmembrane transport Source: GOC
    2. chloride transport Source: MGI

    Keywords - Molecular functioni

    Chloride channel, Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Calcium, Chloride

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anoctamin-2By similarity
    Alternative name(s):
    Transmembrane protein 16BImported
    Gene namesi
    Name:Ano2By similarity
    Synonyms:Tmem16bImported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:2387214. Ano2.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. chloride channel complex Source: UniProtKB-KW
    2. nonmotile primary cilium Source: MGI
    3. nucleus Source: Ensembl
    4. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1000 – 10023Missing: Impaired interaction with DLG4. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10021002Anoctamin-2PRO_0000353187Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi839 – 8391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi847 – 8471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi854 – 8541N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8CFW1.

    PTM databases

    PhosphoSiteiQ8CFW1.

    Expressioni

    Tissue specificityi

    Expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia (at protein level). Also observed in retinal pigment epithelium (RPE), olfactory bulb, brain, and cortex.3 Publications

    Developmental stagei

    Detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. In developing skin, expression is restricted to basal layers of the epidermis at E16.5.1 Publication

    Gene expression databases

    BgeeiQ8CFW1.
    GenevestigatoriQ8CFW1.

    Interactioni

    Subunit structurei

    Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminal region.1 Publication

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 364364CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini386 – 43348ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini455 – 53682CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini558 – 58023ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini602 – 62120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini643 – 746104ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini768 – 79932CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini821 – 90585ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini927 – 100276CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei365 – 38521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei434 – 45421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei537 – 55721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei581 – 60121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei622 – 64221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei747 – 76721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei800 – 82021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei906 – 92621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1000 – 10023DLG4 binding (PDZ)

    Sequence similaritiesi

    Belongs to the anoctamin family.By similarity

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG320103.
    GeneTreeiENSGT00710000106727.
    HOGENOMiHOG000006509.
    HOVERGENiHBG069519.
    InParanoidiQ8CFW1.
    OMAiDRFPGYL.
    OrthoDBiEOG7BS48W.
    TreeFamiTF314265.

    Family and domain databases

    InterProiIPR007632. Anoctamin.
    [Graphical view]
    PANTHERiPTHR12308. PTHR12308. 1 hit.
    PfamiPF04547. Anoctamin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CFW1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPGLRDIP LLPGSPRRLS SRTVARGSQG PKHGQQYLKV PGHRAPGQRD     50
    NSSLHPSQVS RRESSRDRSV INNYLDANEP PSSEARLSRM HFHDNQRKVD 100
    YVLAYHYRKR GAHLGHGSPG HSLAVISNGE TGKERHGGGP GDVELGPLDA 150
    LEEERREQRD EFEHNLMAAG LELEKDLESK SQGSVFVRIH APWQVLAREA 200
    EFLKIKVPTK KMYEIKAGGS IAKKFSAILQ TLSSPLQPRV PEHSNNRMKN 250
    LSYPFSREKM YLYNIQEKDT FFDNATRSRI VHEILKRTAC SRANNTMGIN 300
    SLIANNIYEA AYPLHDGEYD SPGDDMNDRK LLYQEWARYG VFYKFQPIDL 350
    IRKYFGEKIG LYFAWLGLYT SFLIPSSVIG VIVFLYGCAT IEEDIPSKEM 400
    CDHQNAFTMC PLCDKSCDYW NLSSACGTAR ASHLFDNPAT VFFSIFMALW 450
    ATMFLENWKR LQMRLGYFWD LTGIEEEEER SQEHSRPEYE TKVREKLLKE 500
    SGKSAVQKLE ANSPEDDEDD EDKLTWKDRF PGYLMNFASI LFMIALTFSI 550
    VFGVIVYRIT TAAALSLNKA TRSNVRVTVT ATAVIINLVV ILILDEIYGA 600
    VAKWLTKIEV PKTEQTFEER LILKAFLLKF VNAYSPIFYV AFFKGRFVGR 650
    PGSYVYVFDG YRMEECAPGG CLMELCIQLS IIMLGKQLIQ NNIFEIGVPK 700
    LKKLFRKLKD ETEPGESDPD HSKRPEQWDL DHSLEPYTGL TPEYMEMIIQ 750
    FGFVTLFVAS FPLAPVFALL NNVIEVRLDA KKFVTELRRP DAVRTKDIGI 800
    WFDILSGIGK FSVIINAFVI AVTSDFIPRL VYQYSYSHNG TLHGFVNHTL 850
    SFFNVSQLKE GTQPENSQFD QEVQFCRFKD YREPPWAPNP YEFSKQYWSV 900
    LSARLAFVII FQNLVMFLSV LVDWMIPDIP TDISDQIKKE KSLLVDFFLK 950
    EEHEKVKLAD EPTQRSQGGG DRSRRSRAAS SAPSGRSQPG SIASSGSQHT 1000
    NV 1002
    Length:1,002
    Mass (Da):114,134
    Last modified:November 3, 2009 - v2
    Checksum:i8514FC9CC13F78C7
    GO

    Sequence cautioni

    The sequence BAC32073.1 differs from that shown. Reason: Probable cloning artifact.
    The sequence AAH33409.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ384096 mRNA. Translation: ACL36051.1.
    AK044763 mRNA. Translation: BAC32073.1. Sequence problems.
    BC033409 mRNA. Translation: AAH33409.1. Different initiation.
    CCDSiCCDS39643.2.
    RefSeqiNP_705817.2. NM_153589.2.
    XP_006506182.1. XM_006506119.1.
    UniGeneiMm.333398.

    Genome annotation databases

    EnsembliENSMUST00000160496; ENSMUSP00000125303; ENSMUSG00000038115.
    GeneIDi243634.
    KEGGimmu:243634.
    UCSCiuc009duv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ384096 mRNA. Translation: ACL36051.1 .
    AK044763 mRNA. Translation: BAC32073.1 . Sequence problems.
    BC033409 mRNA. Translation: AAH33409.1 . Different initiation.
    CCDSi CCDS39643.2.
    RefSeqi NP_705817.2. NM_153589.2.
    XP_006506182.1. XM_006506119.1.
    UniGenei Mm.333398.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8CFW1.

    Proteomic databases

    PRIDEi Q8CFW1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000160496 ; ENSMUSP00000125303 ; ENSMUSG00000038115 .
    GeneIDi 243634.
    KEGGi mmu:243634.
    UCSCi uc009duv.1. mouse.

    Organism-specific databases

    CTDi 57101.
    MGIi MGI:2387214. Ano2.

    Phylogenomic databases

    eggNOGi NOG320103.
    GeneTreei ENSGT00710000106727.
    HOGENOMi HOG000006509.
    HOVERGENi HBG069519.
    InParanoidi Q8CFW1.
    OMAi DRFPGYL.
    OrthoDBi EOG7BS48W.
    TreeFami TF314265.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 385867.
    PROi Q8CFW1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CFW1.
    Genevestigatori Q8CFW1.

    Family and domain databases

    InterProi IPR007632. Anoctamin.
    [Graphical view ]
    PANTHERi PTHR12308. PTHR12308. 1 hit.
    Pfami PF04547. Anoctamin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals."
      Stoehr H., Heisig J.B., Benz P.M., Schoeberl S., Milenkovic V.M., Strauss O., Aartsen W.M., Wijnholds J., Weber B.H.F., Schulz H.L.
      J. Neurosci. 29:6809-6818(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH DLG4, MUTAGENESIS OF 1000-THR--ASN-1002, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Retina.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-543.
      Strain: C57BL/6JImported.
      Tissue: RetinaImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-1002.
      Strain: C57BL/6Imported.
      Tissue: EyeImported.
    4. "Expression of TMEM16 paralogs during murine embryogenesis."
      Rock J.R., Harfe B.D.
      Dev. Dyn. 237:2566-2574(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "TMEM16B induces chloride currents activated by calcium in mammalian cells."
      Pifferi S., Dibattista M., Menini A.
      Pflugers Arch. 458:1023-1038(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION.
    6. "ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification."
      Stephan A.B., Shum E.Y., Hirsh S., Cygnar K.D., Reisert J., Zhao H.
      Proc. Natl. Acad. Sci. U.S.A. 106:11776-11781(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. Cited for: TISSUE SPECIFICITY.
    8. "ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins."
      Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.
      Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
      Winpenny J.P., Gray M.A.
      Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    10. "Anoctamin 2/TMEM16B: a calcium-activated chloride channel in olfactory transduction."
      Pifferi S., Cenedese V., Menini A.
      Exp. Physiol. 97:193-199(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, FUNCTION.

    Entry informationi

    Entry nameiANO2_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFW1
    Secondary accession number(s): C4N788, Q8C8N6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 4, 2008
    Last sequence update: November 3, 2009
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3