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Q8CFW1

- ANO2_MOUSE

UniProt

Q8CFW1 - ANO2_MOUSE

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Protein

Anoctamin-2

Gene

Ano2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.5 Publications

Enzyme regulationi

Channel activity is repressed by chloride inhibitors; strongly by niflumic acid (NFA), partially by flufenamic acid (FFA), and only slightly by meclofenamic acid (MFA), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS), and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS).1 Publication

GO - Molecular functioni

  1. intracellular calcium activated chloride channel activity Source: UniProtKB
  2. protein heterodimerization activity Source: MGI
  3. protein homodimerization activity Source: MGI

GO - Biological processi

  1. chloride transmembrane transport Source: GOC
  2. chloride transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride

Enzyme and pathway databases

ReactomeiREACT_196640. Stimuli-sensing channels.
REACT_268398. Orphan transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-2By similarity
Alternative name(s):
Transmembrane protein 16BImported
Gene namesi
Name:Ano2By similarity
Synonyms:Tmem16bImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:2387214. Ano2.

Subcellular locationi

Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 364364CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei365 – 38521HelicalSequence AnalysisAdd
BLAST
Topological domaini386 – 43348ExtracellularSequence AnalysisAdd
BLAST
Transmembranei434 – 45421HelicalSequence AnalysisAdd
BLAST
Topological domaini455 – 53682CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei537 – 55721HelicalSequence AnalysisAdd
BLAST
Topological domaini558 – 58023ExtracellularSequence AnalysisAdd
BLAST
Transmembranei581 – 60121HelicalSequence AnalysisAdd
BLAST
Topological domaini602 – 62120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei622 – 64221HelicalSequence AnalysisAdd
BLAST
Topological domaini643 – 746104ExtracellularSequence AnalysisAdd
BLAST
Transmembranei747 – 76721HelicalSequence AnalysisAdd
BLAST
Topological domaini768 – 79932CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei800 – 82021HelicalSequence AnalysisAdd
BLAST
Topological domaini821 – 90585ExtracellularSequence AnalysisAdd
BLAST
Transmembranei906 – 92621HelicalSequence AnalysisAdd
BLAST
Topological domaini927 – 100276CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. chloride channel complex Source: UniProtKB-KW
  2. nonmotile primary cilium Source: MGI
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1000 – 10023Missing: Impaired interaction with DLG4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Anoctamin-2PRO_0000353187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi839 – 8391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi847 – 8471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi854 – 8541N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8CFW1.

PTM databases

PhosphoSiteiQ8CFW1.

Expressioni

Tissue specificityi

Expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia (at protein level). Also observed in retinal pigment epithelium (RPE), olfactory bulb, brain, and cortex.3 Publications

Developmental stagei

Detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. In developing skin, expression is restricted to basal layers of the epidermis at E16.5.1 Publication

Gene expression databases

BgeeiQ8CFW1.
ExpressionAtlasiQ8CFW1. baseline and differential.
GenevestigatoriQ8CFW1.

Interactioni

Subunit structurei

Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminal region.1 Publication

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1000 – 10023DLG4 binding (PDZ)

Sequence similaritiesi

Belongs to the anoctamin family.By similarity

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320103.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ8CFW1.
OMAiDRFPGYL.
OrthoDBiEOG7BS48W.
TreeFamiTF314265.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CFW1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAPGLRDIP LLPGSPRRLS SRTVARGSQG PKHGQQYLKV PGHRAPGQRD
60 70 80 90 100
NSSLHPSQVS RRESSRDRSV INNYLDANEP PSSEARLSRM HFHDNQRKVD
110 120 130 140 150
YVLAYHYRKR GAHLGHGSPG HSLAVISNGE TGKERHGGGP GDVELGPLDA
160 170 180 190 200
LEEERREQRD EFEHNLMAAG LELEKDLESK SQGSVFVRIH APWQVLAREA
210 220 230 240 250
EFLKIKVPTK KMYEIKAGGS IAKKFSAILQ TLSSPLQPRV PEHSNNRMKN
260 270 280 290 300
LSYPFSREKM YLYNIQEKDT FFDNATRSRI VHEILKRTAC SRANNTMGIN
310 320 330 340 350
SLIANNIYEA AYPLHDGEYD SPGDDMNDRK LLYQEWARYG VFYKFQPIDL
360 370 380 390 400
IRKYFGEKIG LYFAWLGLYT SFLIPSSVIG VIVFLYGCAT IEEDIPSKEM
410 420 430 440 450
CDHQNAFTMC PLCDKSCDYW NLSSACGTAR ASHLFDNPAT VFFSIFMALW
460 470 480 490 500
ATMFLENWKR LQMRLGYFWD LTGIEEEEER SQEHSRPEYE TKVREKLLKE
510 520 530 540 550
SGKSAVQKLE ANSPEDDEDD EDKLTWKDRF PGYLMNFASI LFMIALTFSI
560 570 580 590 600
VFGVIVYRIT TAAALSLNKA TRSNVRVTVT ATAVIINLVV ILILDEIYGA
610 620 630 640 650
VAKWLTKIEV PKTEQTFEER LILKAFLLKF VNAYSPIFYV AFFKGRFVGR
660 670 680 690 700
PGSYVYVFDG YRMEECAPGG CLMELCIQLS IIMLGKQLIQ NNIFEIGVPK
710 720 730 740 750
LKKLFRKLKD ETEPGESDPD HSKRPEQWDL DHSLEPYTGL TPEYMEMIIQ
760 770 780 790 800
FGFVTLFVAS FPLAPVFALL NNVIEVRLDA KKFVTELRRP DAVRTKDIGI
810 820 830 840 850
WFDILSGIGK FSVIINAFVI AVTSDFIPRL VYQYSYSHNG TLHGFVNHTL
860 870 880 890 900
SFFNVSQLKE GTQPENSQFD QEVQFCRFKD YREPPWAPNP YEFSKQYWSV
910 920 930 940 950
LSARLAFVII FQNLVMFLSV LVDWMIPDIP TDISDQIKKE KSLLVDFFLK
960 970 980 990 1000
EEHEKVKLAD EPTQRSQGGG DRSRRSRAAS SAPSGRSQPG SIASSGSQHT

NV
Length:1,002
Mass (Da):114,134
Last modified:November 3, 2009 - v2
Checksum:i8514FC9CC13F78C7
GO

Sequence cautioni

The sequence AAH33409.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC32073.1 differs from that shown. Reason: Probable cloning artifact.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ384096 mRNA. Translation: ACL36051.1.
AK044763 mRNA. Translation: BAC32073.1. Sequence problems.
BC033409 mRNA. Translation: AAH33409.1. Different initiation.
CCDSiCCDS39643.2.
RefSeqiNP_705817.2. NM_153589.2.
XP_006506182.1. XM_006506119.1.
UniGeneiMm.333398.

Genome annotation databases

EnsembliENSMUST00000160496; ENSMUSP00000125303; ENSMUSG00000038115.
GeneIDi243634.
KEGGimmu:243634.
UCSCiuc009duv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ384096 mRNA. Translation: ACL36051.1 .
AK044763 mRNA. Translation: BAC32073.1 . Sequence problems.
BC033409 mRNA. Translation: AAH33409.1 . Different initiation.
CCDSi CCDS39643.2.
RefSeqi NP_705817.2. NM_153589.2.
XP_006506182.1. XM_006506119.1.
UniGenei Mm.333398.

3D structure databases

ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8CFW1.

Proteomic databases

PRIDEi Q8CFW1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000160496 ; ENSMUSP00000125303 ; ENSMUSG00000038115 .
GeneIDi 243634.
KEGGi mmu:243634.
UCSCi uc009duv.1. mouse.

Organism-specific databases

CTDi 57101.
MGIi MGI:2387214. Ano2.

Phylogenomic databases

eggNOGi NOG320103.
GeneTreei ENSGT00760000119015.
HOGENOMi HOG000006509.
HOVERGENi HBG069519.
InParanoidi Q8CFW1.
OMAi DRFPGYL.
OrthoDBi EOG7BS48W.
TreeFami TF314265.

Enzyme and pathway databases

Reactomei REACT_196640. Stimuli-sensing channels.
REACT_268398. Orphan transporters.

Miscellaneous databases

ChiTaRSi Ano2. mouse.
NextBioi 385867.
PROi Q8CFW1.
SOURCEi Search...

Gene expression databases

Bgeei Q8CFW1.
ExpressionAtlasi Q8CFW1. baseline and differential.
Genevestigatori Q8CFW1.

Family and domain databases

InterProi IPR007632. Anoctamin.
[Graphical view ]
PANTHERi PTHR12308. PTHR12308. 1 hit.
Pfami PF04547. Anoctamin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals."
    Stoehr H., Heisig J.B., Benz P.M., Schoeberl S., Milenkovic V.M., Strauss O., Aartsen W.M., Wijnholds J., Weber B.H.F., Schulz H.L.
    J. Neurosci. 29:6809-6818(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH DLG4, MUTAGENESIS OF 1000-THR--ASN-1002, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Retina.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-543.
    Strain: C57BL/6JImported.
    Tissue: RetinaImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-1002.
    Strain: C57BL/6Imported.
    Tissue: EyeImported.
  4. "Expression of TMEM16 paralogs during murine embryogenesis."
    Rock J.R., Harfe B.D.
    Dev. Dyn. 237:2566-2574(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "TMEM16B induces chloride currents activated by calcium in mammalian cells."
    Pifferi S., Dibattista M., Menini A.
    Pflugers Arch. 458:1023-1038(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  6. "ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification."
    Stephan A.B., Shum E.Y., Hirsh S., Cygnar K.D., Reisert J., Zhao H.
    Proc. Natl. Acad. Sci. U.S.A. 106:11776-11781(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. Cited for: TISSUE SPECIFICITY.
  8. "ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins."
    Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.
    Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
    Winpenny J.P., Gray M.A.
    Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. "Anoctamin 2/TMEM16B: a calcium-activated chloride channel in olfactory transduction."
    Pifferi S., Cenedese V., Menini A.
    Exp. Physiol. 97:193-199(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, FUNCTION.

Entry informationi

Entry nameiANO2_MOUSE
AccessioniPrimary (citable) accession number: Q8CFW1
Secondary accession number(s): C4N788, Q8C8N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: November 3, 2009
Last modified: November 26, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3