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Q8CFW1 (ANO2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anoctamin-2
Alternative name(s):
Transmembrane protein 16B
Gene names
Name:Ano2
Synonyms:Tmem16b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1002 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina. Ref.1 Ref.5 Ref.6 Ref.8 Ref.10

Enzyme regulation

Channel activity is repressed by chloride inhibitors; strongly by niflumic acid (NFA), partially by flufenamic acid (FFA), and only slightly by meclofenamic acid (MFA), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS), and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Ref.5

Subunit structure

Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminal region. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1 Ref.6.

Tissue specificity

Expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia (at protein level). Also observed in retinal pigment epithelium (RPE), olfactory bulb, brain, and cortex. Ref.1 Ref.6 Ref.7

Developmental stage

Detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. In developing skin, expression is restricted to basal layers of the epidermis at E16.5. Ref.4

Post-translational modification

Glycosylated. Ref.1

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Sequence similarities

Belongs to the anoctamin family. UniProtKB Q9NQ90

Sequence caution

The sequence AAH33409.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC32073.1 differs from that shown. Reason: Probable cloning artifact.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10021002Anoctamin-2
PRO_0000353187

Regions

Topological domain1 – 364364Cytoplasmic Potential
Transmembrane365 – 38521Helical; Potential
Topological domain386 – 43348Extracellular Potential
Transmembrane434 – 45421Helical; Potential
Topological domain455 – 53682Cytoplasmic Potential
Transmembrane537 – 55721Helical; Potential
Topological domain558 – 58023Extracellular Potential
Transmembrane581 – 60121Helical; Potential
Topological domain602 – 62120Cytoplasmic Potential
Transmembrane622 – 64221Helical; Potential
Topological domain643 – 746104Extracellular Potential
Transmembrane747 – 76721Helical; Potential
Topological domain768 – 79932Cytoplasmic Potential
Transmembrane800 – 82021Helical; Potential
Topological domain821 – 90585Extracellular Potential
Transmembrane906 – 92621Helical; Potential
Topological domain927 – 100276Cytoplasmic Potential
Motif1000 – 10023DLG4 binding (PDZ)

Amino acid modifications

Glycosylation4211N-linked (GlcNAc...) Potential
Glycosylation8391N-linked (GlcNAc...) Potential
Glycosylation8471N-linked (GlcNAc...) Potential
Glycosylation8541N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis1000 – 10023Missing: Impaired interaction with DLG4. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8CFW1 [UniParc].

Last modified November 3, 2009. Version 2.
Checksum: 8514FC9CC13F78C7

FASTA1,002114,134
        10         20         30         40         50         60 
MAAPGLRDIP LLPGSPRRLS SRTVARGSQG PKHGQQYLKV PGHRAPGQRD NSSLHPSQVS 

        70         80         90        100        110        120 
RRESSRDRSV INNYLDANEP PSSEARLSRM HFHDNQRKVD YVLAYHYRKR GAHLGHGSPG 

       130        140        150        160        170        180 
HSLAVISNGE TGKERHGGGP GDVELGPLDA LEEERREQRD EFEHNLMAAG LELEKDLESK 

       190        200        210        220        230        240 
SQGSVFVRIH APWQVLAREA EFLKIKVPTK KMYEIKAGGS IAKKFSAILQ TLSSPLQPRV 

       250        260        270        280        290        300 
PEHSNNRMKN LSYPFSREKM YLYNIQEKDT FFDNATRSRI VHEILKRTAC SRANNTMGIN 

       310        320        330        340        350        360 
SLIANNIYEA AYPLHDGEYD SPGDDMNDRK LLYQEWARYG VFYKFQPIDL IRKYFGEKIG 

       370        380        390        400        410        420 
LYFAWLGLYT SFLIPSSVIG VIVFLYGCAT IEEDIPSKEM CDHQNAFTMC PLCDKSCDYW 

       430        440        450        460        470        480 
NLSSACGTAR ASHLFDNPAT VFFSIFMALW ATMFLENWKR LQMRLGYFWD LTGIEEEEER 

       490        500        510        520        530        540 
SQEHSRPEYE TKVREKLLKE SGKSAVQKLE ANSPEDDEDD EDKLTWKDRF PGYLMNFASI 

       550        560        570        580        590        600 
LFMIALTFSI VFGVIVYRIT TAAALSLNKA TRSNVRVTVT ATAVIINLVV ILILDEIYGA 

       610        620        630        640        650        660 
VAKWLTKIEV PKTEQTFEER LILKAFLLKF VNAYSPIFYV AFFKGRFVGR PGSYVYVFDG 

       670        680        690        700        710        720 
YRMEECAPGG CLMELCIQLS IIMLGKQLIQ NNIFEIGVPK LKKLFRKLKD ETEPGESDPD 

       730        740        750        760        770        780 
HSKRPEQWDL DHSLEPYTGL TPEYMEMIIQ FGFVTLFVAS FPLAPVFALL NNVIEVRLDA 

       790        800        810        820        830        840 
KKFVTELRRP DAVRTKDIGI WFDILSGIGK FSVIINAFVI AVTSDFIPRL VYQYSYSHNG 

       850        860        870        880        890        900 
TLHGFVNHTL SFFNVSQLKE GTQPENSQFD QEVQFCRFKD YREPPWAPNP YEFSKQYWSV 

       910        920        930        940        950        960 
LSARLAFVII FQNLVMFLSV LVDWMIPDIP TDISDQIKKE KSLLVDFFLK EEHEKVKLAD 

       970        980        990       1000 
EPTQRSQGGG DRSRRSRAAS SAPSGRSQPG SIASSGSQHT NV 

« Hide

References

« Hide 'large scale' references
[1]"TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals."
Stoehr H., Heisig J.B., Benz P.M., Schoeberl S., Milenkovic V.M., Strauss O., Aartsen W.M., Wijnholds J., Weber B.H.F., Schulz H.L.
J. Neurosci. 29:6809-6818(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH DLG4, MUTAGENESIS OF 1000-THR--ASN-1002, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Retina.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-543.
Strain: C57BL/6J.
Tissue: Retina.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-1002.
Strain: C57BL/6.
Tissue: Eye.
[4]"Expression of TMEM16 paralogs during murine embryogenesis."
Rock J.R., Harfe B.D.
Dev. Dyn. 237:2566-2574(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"TMEM16B induces chloride currents activated by calcium in mammalian cells."
Pifferi S., Dibattista M., Menini A.
Pflugers Arch. 458:1023-1038(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[6]"ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification."
Stephan A.B., Shum E.Y., Hirsh S., Cygnar K.D., Reisert J., Zhao H.
Proc. Natl. Acad. Sci. U.S.A. 106:11776-11781(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"Expression and function of epithelial anoctamins."
Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M., Martins J.R., Kunzelmann K.
J. Biol. Chem. 285:7838-7845(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins."
Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.
Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
Winpenny J.P., Gray M.A.
Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"Anoctamin 2/TMEM16B: a calcium-activated chloride channel in olfactory transduction."
Pifferi S., Cenedese V., Menini A.
Exp. Physiol. 97:193-199(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FJ384096 mRNA. Translation: ACL36051.1.
AK044763 mRNA. Translation: BAC32073.1. Sequence problems.
BC033409 mRNA. Translation: AAH33409.1. Different initiation.
CCDSCCDS39643.2.
RefSeqNP_705817.2. NM_153589.2.
XP_006506182.1. XM_006506119.1.
UniGeneMm.333398.

3D structure databases

ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8CFW1.

Proteomic databases

PRIDEQ8CFW1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000160496; ENSMUSP00000125303; ENSMUSG00000038115.
GeneID243634.
KEGGmmu:243634.
UCSCuc009duv.1. mouse.

Organism-specific databases

CTD57101.
MGIMGI:2387214. Ano2.

Phylogenomic databases

eggNOGNOG320103.
GeneTreeENSGT00710000106727.
HOGENOMHOG000006509.
HOVERGENHBG069519.
InParanoidQ8CFW1.
OMADRFPGYL.
OrthoDBEOG7BS48W.
TreeFamTF314265.

Gene expression databases

BgeeQ8CFW1.
GenevestigatorQ8CFW1.

Family and domain databases

InterProIPR007632. Anoctamin.
[Graphical view]
PANTHERPTHR12308. PTHR12308. 1 hit.
PfamPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio385867.
PROQ8CFW1.
SOURCESearch...

Entry information

Entry nameANO2_MOUSE
AccessionPrimary (citable) accession number: Q8CFW1
Secondary accession number(s): C4N788, Q8C8N6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: November 3, 2009
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot