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Q8CFV9

- RIFK_MOUSE

UniProt

Q8CFV9 - RIFK_MOUSE

Protein

Riboflavin kinase

Gene

Rfk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase By similarity.By similarity

    Catalytic activityi

    ATP + riboflavin = ADP + FMN.

    Cofactori

    Zinc or magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenBy similarity
    Binding sitei21 – 211ATP; via amide nitrogenBy similarity
    Metal bindingi27 – 271MagnesiumBy similarity
    Binding sitei27 – 271ATP; via amide nitrogenBy similarity
    Binding sitei29 – 291ATPBy similarity
    Active sitei79 – 791NucleophileBy similarity
    Binding sitei82 – 821ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei84 – 841ATP; via carbonyl oxygenBy similarity
    Binding sitei91 – 911ATPBy similarity
    Binding sitei104 – 1041FMNBy similarity
    Binding sitei107 – 1071FMN; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei109 – 1091FMN; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. riboflavin kinase activity Source: MGI

    GO - Biological processi

    1. FMN biosynthetic process Source: UniProtKB-UniPathway
    2. positive regulation of NAD(P)H oxidase activity Source: MGI
    3. reactive oxygen species metabolic process Source: MGI
    4. riboflavin biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_216155. Vitamin B2 (riboflavin) metabolism.
    UniPathwayiUPA00276; UER00406.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin kinase (EC:2.7.1.26)
    Alternative name(s):
    ATP:riboflavin 5'-phosphotransferase
    Flavokinase
    KOI-4
    Gene namesi
    Name:Rfk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1914688. Rfk.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mutant embryos die in utero before 7.5 dpc.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 155155Riboflavin kinasePRO_0000194149Add
    BLAST

    Proteomic databases

    MaxQBiQ8CFV9.
    PaxDbiQ8CFV9.
    PRIDEiQ8CFV9.

    PTM databases

    PhosphoSiteiQ8CFV9.

    Expressioni

    Gene expression databases

    BgeeiQ8CFV9.
    CleanExiMM_RFK.
    GenevestigatoriQ8CFV9.

    Interactioni

    Subunit structurei

    Monomer By similarity. Directly interacts with TNFRSF1A death domain; this interaction may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA.By similarity1 Publication

    Protein-protein interaction databases

    DIPiDIP-60455N.
    STRINGi10090.ENSMUSP00000025617.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFV9.
    SMRiQ8CFV9. Positions 2-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG0196.
    GeneTreeiENSGT00390000015537.
    HOGENOMiHOG000260803.
    HOVERGENiHBG049989.
    InParanoidiQ8CFV9.
    KOiK00861.
    OMAiSLPYFCR.
    OrthoDBiEOG7K3TP0.
    PhylomeDBiQ8CFV9.
    TreeFamiTF313786.

    Family and domain databases

    Gene3Di2.40.30.30. 1 hit.
    InterProiIPR023468. Riboflavin_kinase.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    [Graphical view]
    PANTHERiPTHR22749. PTHR22749. 1 hit.
    PfamiPF01687. Flavokinase. 1 hit.
    [Graphical view]
    SMARTiSM00904. Flavokinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF82114. SSF82114. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8CFV9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSLPFFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DVSTGIYYGW    50
    ASVGSGDVHK MVVSIGWNPY YKNVKKSMET HIIHTFKEDF YGEILNVAIV 100
    GYLRPEKNFD SLESLISAIQ GDIEEAKKQL DLPEHLKLKD DNFFQVSKGK 150
    IMNGH 155
    Length:155
    Mass (Da):17,437
    Last modified:October 3, 2003 - v2
    Checksum:iAE6577023E3D34CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK010607 mRNA. Translation: BAB27057.1.
    AK002806 mRNA. Translation: BAB22372.1.
    AK008352 mRNA. Translation: BAB25622.1.
    BC033521 mRNA. Translation: AAH33521.2.
    BC051021 mRNA. Translation: AAH51021.1.
    AF031380 Genomic DNA. Translation: AAB86494.1.
    AF031381 Genomic DNA. Translation: AAB86495.1.
    CCDSiCCDS29688.1.
    RefSeqiNP_062310.1. NM_019437.3.
    UniGeneiMm.7013.

    Genome annotation databases

    EnsembliENSMUST00000025617; ENSMUSP00000025617; ENSMUSG00000024712.
    GeneIDi54391.
    KEGGimmu:54391.
    UCSCiuc008gxo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK010607 mRNA. Translation: BAB27057.1 .
    AK002806 mRNA. Translation: BAB22372.1 .
    AK008352 mRNA. Translation: BAB25622.1 .
    BC033521 mRNA. Translation: AAH33521.2 .
    BC051021 mRNA. Translation: AAH51021.1 .
    AF031380 Genomic DNA. Translation: AAB86494.1 .
    AF031381 Genomic DNA. Translation: AAB86495.1 .
    CCDSi CCDS29688.1.
    RefSeqi NP_062310.1. NM_019437.3.
    UniGenei Mm.7013.

    3D structure databases

    ProteinModelPortali Q8CFV9.
    SMRi Q8CFV9. Positions 2-148.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60455N.
    STRINGi 10090.ENSMUSP00000025617.

    PTM databases

    PhosphoSitei Q8CFV9.

    Proteomic databases

    MaxQBi Q8CFV9.
    PaxDbi Q8CFV9.
    PRIDEi Q8CFV9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025617 ; ENSMUSP00000025617 ; ENSMUSG00000024712 .
    GeneIDi 54391.
    KEGGi mmu:54391.
    UCSCi uc008gxo.2. mouse.

    Organism-specific databases

    CTDi 55312.
    MGIi MGI:1914688. Rfk.

    Phylogenomic databases

    eggNOGi COG0196.
    GeneTreei ENSGT00390000015537.
    HOGENOMi HOG000260803.
    HOVERGENi HBG049989.
    InParanoidi Q8CFV9.
    KOi K00861.
    OMAi SLPYFCR.
    OrthoDBi EOG7K3TP0.
    PhylomeDBi Q8CFV9.
    TreeFami TF313786.

    Enzyme and pathway databases

    UniPathwayi UPA00276 ; UER00406 .
    Reactomei REACT_216155. Vitamin B2 (riboflavin) metabolism.

    Miscellaneous databases

    ChiTaRSi RFK. mouse.
    NextBioi 311232.
    PROi Q8CFV9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CFV9.
    CleanExi MM_RFK.
    Genevestigatori Q8CFV9.

    Family and domain databases

    Gene3Di 2.40.30.30. 1 hit.
    InterProi IPR023468. Riboflavin_kinase.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    [Graphical view ]
    PANTHERi PTHR22749. PTHR22749. 1 hit.
    Pfami PF01687. Flavokinase. 1 hit.
    [Graphical view ]
    SMARTi SM00904. Flavokinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82114. SSF82114. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell, Kidney and Small intestine.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium and Retina.
    3. Abrantes E.F., Silva A.M., Reis L.F.L.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 79-112 AND 114-155.
      Strain: 129/Sv.
    4. Cited for: INTERACTION WITH CYBA; TNFRSF1A AND TRADD, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRIFK_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFV9
    Secondary accession number(s): O35471, O35472, Q9CQ95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: October 3, 2003
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3