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Q8CFT2 (SET1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase SETD1B

EC=2.1.1.43
Alternative name(s):
SET domain-containing protein 1B
Gene names
Name:Setd1b
Synonyms:Kiaa1076, Set1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1985 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subunit structure

Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with HCFC1. and ASH2L/ASH2. Interacts (via the RRM domain) with WDR82. Interacts (via the RRM domain) with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) only in the presence of WDR82. Binds specifically to CTD heptad repeats phosphorylated on 'Ser-5' of each heptad. Interacts with RBM15 By similarity.

Subcellular location

Nucleus speckle By similarity. Chromosome By similarity. Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SETD1B each bind to a unique set of target genes By similarity.

Tissue specificity

Widely expressed. Ref.4

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Contains 1 post-SET domain.

Contains 1 RRM (RNA recognition motif) domain.

Contains 1 SET domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CFT2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CFT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1139-1179: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19851985Histone-lysine N-methyltransferase SETD1B
PRO_0000316994

Regions

Domain92 – 18089RRM
Domain1846 – 1963118SET
Domain1969 – 198517Post-SET
Compositional bias373 – 16901318Pro-rich
Compositional bias1031 – 1179149Ser-rich
Compositional bias1059 – 1331273Glu-rich

Amino acid modifications

Modified residue16781Phosphoserine By similarity
Modified residue16821Phosphoserine By similarity

Natural variations

Alternative sequence1139 – 117941Missing in isoform 2.
VSP_030851

Experimental info

Sequence conflict12221A → V in BAC65717. Ref.3
Sequence conflict14111S → G in BAC65717. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: 760EA261769292EC

FASTA1,985215,352
        10         20         30         40         50         60 
MENSHPHHHH QQPPPQPGPS GERRNHHWRS YKLMIDPALK KGHHKLYRYD GQHFSLAMSS 

        70         80         90        100        110        120 
NRPVEIVEDP RVVGIWTKNK ELELSVPKFK IDEFYVGPVP PKQVTFAKLN DNVRENFLRD 

       130        140        150        160        170        180 
MCKKYGEVEE VEILYNPKTK KHLGIAKVVF ATVRGAKEAV QHLHSTSVMG NIIHVELDTK 

       190        200        210        220        230        240 
GETRMRFYEL LVTGRYTPQT LPVGELDAIS PIVSETLQLS DALKRLKDGS LSAGCGSGSS 

       250        260        270        280        290        300 
SVTPNSGGTP FSQDTAYSSC RLDTPNSYGQ GTPITPRLGT PFSQDSSYSS RQPTPSYLFS 

       310        320        330        340        350        360 
QDPTATFKAR RHESKFTDAY NRRHEHHYVH NSAVAGATAP FRGSSDLSFG TVGSSGTPFK 

       370        380        390        400        410        420 
AQSQDATTFA HTPPPAQTAT ASGFKSAFSP YQTPAPPFPP PPEEPTATAA FGSRDSGEFR 

       430        440        450        460        470        480 
RAPAPPPLPP AEPPAKEKPG TPPGPPPPDS NSMELGGRPT FGWSPEPCDS PGTPTLESSP 

       490        500        510        520        530        540 
AGPEKPHDSL DSRIEMLLKE QRTKLPFLRE QDSDTEIQME GSPISSSSSQ LSPLSHFGTN 

       550        560        570        580        590        600 
SQPGFRGPSP PSSRPSSTGL EDISPTPLPD SDEDEDLGLG LGPRPPPEPG PPDPMGLLGQ 

       610        620        630        640        650        660 
TAEVDLDLAG DRTPTSERMD EGQQSSGEDM EISDDEMPSA PITSADCPKP MVVTPGAGAV 

       670        680        690        700        710        720 
AAPNVLAPNL PLPPPPGFPP LPPPPPPPPP QPGFPMPPPL PPPPPPPPPA HPAVTVPPPP 

       730        740        750        760        770        780 
LPAPPGVPPP PILPPLPPFP PGLFPVMQVD MSHVLGGQWG GMPMSFQMQT QMLSRLMTGQ 

       790        800        810        820        830        840 
GACPYPPFMA AAAAAASAGL QFVNLPPYRS PFSLSNSGPG RGQHWPPLPK FDPSVPPPGY 

       850        860        870        880        890        900 
IPRQEDPHKA TVDGVLLVVL KELKAIMKRD LNRKMVEVVA FRAFDEWWDK KERMAKASLT 

       910        920        930        940        950        960 
PVKSGEHKDE DRPKPKDRIA SCLLESWGKG EGLGYEGLGL GIGLRGAIRL PSFKVKRKEP 

       970        980        990       1000       1010       1020 
PDTASSGDQK RLRPSTSVDE EDEESERERD RDIADAPCEL TKRDPKSVGV RRRPGRPLEL 

      1030       1040       1050       1060       1070       1080 
DSGGEEDEKE SLSASSSSSA SSSSGSSTTS PSSSASDKEE EDRESTEEEE EEEEEEAEEE 

      1090       1100       1110       1120       1130       1140 
EEEGPRSRIS SPSSSSSSDK DDEDDNEADS DGQIDSDIDD QGAPLSEASE KDNGDSEEEE 

      1150       1160       1170       1180       1190       1200 
TESITTSKAP AESSSSSSES SGSSEFESSS ESESSSSSSE DEEEMTVPGV EEEEEEEEEE 

      1210       1220       1230       1240       1250       1260 
EKETAMAAAT VVAMAEESMP PAGGQDFEQD RAEVPLGPRG PMRESLGTEE EVDIEAEDEV 

      1270       1280       1290       1300       1310       1320 
PEMQAPELEE PPLPMGARKL EGSPEPPEEP GPNTQGDMLL SPELPARETE EAQLPSPPEH 

      1330       1340       1350       1360       1370       1380 
GPESDLDMEP EPPPMLSLPL QPPLPPPRLL RPPSPPPEPE TPEPPKPPVP LEPPPEDHPP 

      1390       1400       1410       1420       1430       1440 
RTPGLCGSLA KSQSTETVPA TPGGEPPLSG SSSGLSLSSP QVPGSPFSYP SPSPGLSSGG 

      1450       1460       1470       1480       1490       1500 
LPRTPGRDFS FTPTFPEPSG PLLLPVCPLP TGRRDERTGP LASPVLLETG LPLPLPLPLP 

      1510       1520       1530       1540       1550       1560 
LPLALPVPVL RAQPRPPPQL PPLLPATLAP CPTPIKRKPG RPRRSPPSML SLDGPLVRPP 

      1570       1580       1590       1600       1610       1620 
PGPALGRDLL LLPGQPPAPI FPSAHDPRAV TLDFRNTGIP APPPPLPPQP PPPPPPPPVE 

      1630       1640       1650       1660       1670       1680 
STKLPFKELD NQWPSEAIPP GPRRDEVTEE YVDLAKVRGP WRRPPKKRHE DLVAPSASPE 

      1690       1700       1710       1720       1730       1740 
PSPPQPLFRP RSEFEEMTIL YDIWNGGIDE EDIRFLCVTY ERLLQQDNGM DWLNDTLWVY 

      1750       1760       1770       1780       1790       1800 
HPSTSLSSAK KKKREDGIRE HVTGCARSEG FYTIDKKDKL RYLNSSRAST DEPPMDTQGM 

      1810       1820       1830       1840       1850       1860 
SIPAQPHAST RAGSERRSEQ RRLLSSFTGS CDSDLLKFNQ LKFRKKKLKF CKSHIHDWGL 

      1870       1880       1890       1900       1910       1920 
FAMEPIAADE MVIEYVGQNI RQVIADMREK RYEDEGIGSS YMFRVDHDTI IDATKCGNFA 

      1930       1940       1950       1960       1970       1980 
RFINHSCNPN CYAKVITVES QKKIVIYSKQ HINVNEEITY DYKFPIEDVK IPCLCGSENC 


RGTLN 

« Hide

Isoform 2 [UniParc].

Checksum: E2BD1B70C876448C
Show »

FASTA1,944211,286

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 883-1985 (ISOFORM 1).
Strain: FVB/N.
Tissue: Eye and Mammary tumor.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1090-1985 (ISOFORM 2).
[4]"Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex."
Lee J.-H., Tate C.M., You J.-S., Skalnik D.G.
J. Biol. Chem. 282:13419-13428(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC158114 Genomic DNA. No translation available.
BC038367 mRNA. Translation: AAH38367.2.
BC040775 mRNA. Translation: AAH40775.1.
BC041681 mRNA. Translation: AAH41681.1.
AK122435 mRNA. Translation: BAC65717.1.
RefSeqNP_001035488.2. NM_001040398.2.
UniGeneMm.250391.

3D structure databases

ProteinModelPortalQ8CFT2.
SMRQ8CFT2. Positions 97-202.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8CFT2.

Proteomic databases

PaxDbQ8CFT2.
PRIDEQ8CFT2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000056053; ENSMUSP00000134686; ENSMUSG00000038384. [Q8CFT2-1]
ENSMUST00000163030; ENSMUSP00000133933; ENSMUSG00000038384. [Q8CFT2-1]
ENSMUST00000174836; ENSMUSP00000134461; ENSMUSG00000038384. [Q8CFT2-2]
GeneID208043.
KEGGmmu:208043.

Organism-specific databases

CTD23067.
MGIMGI:2652820. Setd1b.
RougeSearch...

Phylogenomic databases

eggNOGCOG2940.
GeneTreeENSGT00740000115089.
HOVERGENHBG055596.
InParanoidQ8CFT2.
KOK11422.
OMAPVMQVDM.
OrthoDBEOG7GQXTT.
TreeFamTF106436.

Gene expression databases

ArrayExpressQ8CFT2.
BgeeQ8CFT2.
CleanExMM_SETD1B.
GenevestigatorQ8CFT2.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR024657. COMPASS_Set1_N-SET.
IPR015722. Histone-lysine_MeTfrase.
IPR012677. Nucleotide-bd_a/b_plait.
IPR003616. Post-SET_dom.
IPR000504. RRM_dom.
IPR001214. SET_dom.
[Graphical view]
PANTHERPTHR22884:SF10. PTHR22884:SF10. 1 hit.
PfamPF11764. N-SET. 1 hit.
PF00076. RRM_1. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00508. PostSET. 1 hit.
SM00360. RRM. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
PROSITEPS50868. POST_SET. 1 hit.
PS50102. RRM. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8CFT2.
SOURCESearch...

Entry information

Entry nameSET1B_MOUSE
AccessionPrimary (citable) accession number: Q8CFT2
Secondary accession number(s): Q80TK9, Q8CFQ8, Q8CGD1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot