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Q8CFT2

- SET1B_MOUSE

UniProt

Q8CFT2 - SET1B_MOUSE

Protein

Histone-lysine N-methyltransferase SETD1B

Gene

Setd1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

    GO - Molecular functioni

    1. histone methyltransferase activity (H3-K4 specific) Source: Ensembl
    2. nucleotide binding Source: InterPro
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone-lysine N-methyltransferase SETD1B (EC:2.1.1.43)
    Alternative name(s):
    SET domain-containing protein 1B
    Gene namesi
    Name:Setd1b
    Synonyms:Kiaa1076, Set1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2652820. Setd1b.

    Subcellular locationi

    Nucleus speckle By similarity. Chromosome By similarity
    Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.By similarity

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nuclear speck Source: UniProtKB-SubCell
    3. nucleus Source: MGI
    4. Set1C/COMPASS complex Source: UniProtKB

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19851985Histone-lysine N-methyltransferase SETD1BPRO_0000316994Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1678 – 16781PhosphoserineBy similarity
    Modified residuei1682 – 16821PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8CFT2.
    PRIDEiQ8CFT2.

    PTM databases

    PhosphoSiteiQ8CFT2.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ8CFT2.
    BgeeiQ8CFT2.
    CleanExiMM_SETD1B.
    GenevestigatoriQ8CFT2.

    Interactioni

    Subunit structurei

    Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with HCFC1. and ASH2L/ASH2. Interacts (via the RRM domain) with WDR82. Interacts (via the RRM domain) with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) only in the presence of WDR82. Binds specifically to CTD heptad repeats phosphorylated on 'Ser-5' of each heptad. Interacts with RBM15 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFT2.
    SMRiQ8CFT2. Positions 97-202.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini92 – 18089RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini1846 – 1963118SETPROSITE-ProRule annotationAdd
    BLAST
    Domaini1969 – 198517Post-SETPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi373 – 16901318Pro-richAdd
    BLAST
    Compositional biasi1031 – 1179149Ser-richAdd
    BLAST
    Compositional biasi1059 – 1331273Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
    Contains 1 post-SET domain.PROSITE-ProRule annotation
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
    Contains 1 SET domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2940.
    GeneTreeiENSGT00740000115089.
    HOVERGENiHBG055596.
    InParanoidiQ8CFT2.
    KOiK11422.
    OMAiQHWPPLP.
    OrthoDBiEOG7GQXTT.
    TreeFamiTF106436.

    Family and domain databases

    Gene3Di3.30.70.330. 2 hits.
    InterProiIPR024657. COMPASS_Set1_N-SET.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR003616. Post-SET_dom.
    IPR000504. RRM_dom.
    IPR001214. SET_dom.
    [Graphical view]
    PfamiPF11764. N-SET. 1 hit.
    PF00076. RRM_1. 1 hit.
    PF00856. SET. 1 hit.
    [Graphical view]
    SMARTiSM00508. PostSET. 1 hit.
    SM00360. RRM. 1 hit.
    SM00317. SET. 1 hit.
    [Graphical view]
    PROSITEiPS50868. POST_SET. 1 hit.
    PS50102. RRM. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8CFT2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MENSHPHHHH QQPPPQPGPS GERRNHHWRS YKLMIDPALK KGHHKLYRYD     50
    GQHFSLAMSS NRPVEIVEDP RVVGIWTKNK ELELSVPKFK IDEFYVGPVP 100
    PKQVTFAKLN DNVRENFLRD MCKKYGEVEE VEILYNPKTK KHLGIAKVVF 150
    ATVRGAKEAV QHLHSTSVMG NIIHVELDTK GETRMRFYEL LVTGRYTPQT 200
    LPVGELDAIS PIVSETLQLS DALKRLKDGS LSAGCGSGSS SVTPNSGGTP 250
    FSQDTAYSSC RLDTPNSYGQ GTPITPRLGT PFSQDSSYSS RQPTPSYLFS 300
    QDPTATFKAR RHESKFTDAY NRRHEHHYVH NSAVAGATAP FRGSSDLSFG 350
    TVGSSGTPFK AQSQDATTFA HTPPPAQTAT ASGFKSAFSP YQTPAPPFPP 400
    PPEEPTATAA FGSRDSGEFR RAPAPPPLPP AEPPAKEKPG TPPGPPPPDS 450
    NSMELGGRPT FGWSPEPCDS PGTPTLESSP AGPEKPHDSL DSRIEMLLKE 500
    QRTKLPFLRE QDSDTEIQME GSPISSSSSQ LSPLSHFGTN SQPGFRGPSP 550
    PSSRPSSTGL EDISPTPLPD SDEDEDLGLG LGPRPPPEPG PPDPMGLLGQ 600
    TAEVDLDLAG DRTPTSERMD EGQQSSGEDM EISDDEMPSA PITSADCPKP 650
    MVVTPGAGAV AAPNVLAPNL PLPPPPGFPP LPPPPPPPPP QPGFPMPPPL 700
    PPPPPPPPPA HPAVTVPPPP LPAPPGVPPP PILPPLPPFP PGLFPVMQVD 750
    MSHVLGGQWG GMPMSFQMQT QMLSRLMTGQ GACPYPPFMA AAAAAASAGL 800
    QFVNLPPYRS PFSLSNSGPG RGQHWPPLPK FDPSVPPPGY IPRQEDPHKA 850
    TVDGVLLVVL KELKAIMKRD LNRKMVEVVA FRAFDEWWDK KERMAKASLT 900
    PVKSGEHKDE DRPKPKDRIA SCLLESWGKG EGLGYEGLGL GIGLRGAIRL 950
    PSFKVKRKEP PDTASSGDQK RLRPSTSVDE EDEESERERD RDIADAPCEL 1000
    TKRDPKSVGV RRRPGRPLEL DSGGEEDEKE SLSASSSSSA SSSSGSSTTS 1050
    PSSSASDKEE EDRESTEEEE EEEEEEAEEE EEEGPRSRIS SPSSSSSSDK 1100
    DDEDDNEADS DGQIDSDIDD QGAPLSEASE KDNGDSEEEE TESITTSKAP 1150
    AESSSSSSES SGSSEFESSS ESESSSSSSE DEEEMTVPGV EEEEEEEEEE 1200
    EKETAMAAAT VVAMAEESMP PAGGQDFEQD RAEVPLGPRG PMRESLGTEE 1250
    EVDIEAEDEV PEMQAPELEE PPLPMGARKL EGSPEPPEEP GPNTQGDMLL 1300
    SPELPARETE EAQLPSPPEH GPESDLDMEP EPPPMLSLPL QPPLPPPRLL 1350
    RPPSPPPEPE TPEPPKPPVP LEPPPEDHPP RTPGLCGSLA KSQSTETVPA 1400
    TPGGEPPLSG SSSGLSLSSP QVPGSPFSYP SPSPGLSSGG LPRTPGRDFS 1450
    FTPTFPEPSG PLLLPVCPLP TGRRDERTGP LASPVLLETG LPLPLPLPLP 1500
    LPLALPVPVL RAQPRPPPQL PPLLPATLAP CPTPIKRKPG RPRRSPPSML 1550
    SLDGPLVRPP PGPALGRDLL LLPGQPPAPI FPSAHDPRAV TLDFRNTGIP 1600
    APPPPLPPQP PPPPPPPPVE STKLPFKELD NQWPSEAIPP GPRRDEVTEE 1650
    YVDLAKVRGP WRRPPKKRHE DLVAPSASPE PSPPQPLFRP RSEFEEMTIL 1700
    YDIWNGGIDE EDIRFLCVTY ERLLQQDNGM DWLNDTLWVY HPSTSLSSAK 1750
    KKKREDGIRE HVTGCARSEG FYTIDKKDKL RYLNSSRAST DEPPMDTQGM 1800
    SIPAQPHAST RAGSERRSEQ RRLLSSFTGS CDSDLLKFNQ LKFRKKKLKF 1850
    CKSHIHDWGL FAMEPIAADE MVIEYVGQNI RQVIADMREK RYEDEGIGSS 1900
    YMFRVDHDTI IDATKCGNFA RFINHSCNPN CYAKVITVES QKKIVIYSKQ 1950
    HINVNEEITY DYKFPIEDVK IPCLCGSENC RGTLN 1985
    Length:1,985
    Mass (Da):215,352
    Last modified:February 5, 2008 - v2
    Checksum:i760EA261769292EC
    GO
    Isoform 2 (identifier: Q8CFT2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1139-1179: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,944
    Mass (Da):211,286
    Checksum:iE2BD1B70C876448C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1222 – 12221A → V in BAC65717. (PubMed:12693553)Curated
    Sequence conflicti1411 – 14111S → G in BAC65717. (PubMed:12693553)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1139 – 117941Missing in isoform 2. 1 PublicationVSP_030851Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC158114 Genomic DNA. No translation available.
    BC038367 mRNA. Translation: AAH38367.2.
    BC040775 mRNA. Translation: AAH40775.1.
    BC041681 mRNA. Translation: AAH41681.1.
    AK122435 mRNA. Translation: BAC65717.1.
    CCDSiCCDS59684.1. [Q8CFT2-1]
    RefSeqiNP_001035488.2. NM_001040398.2. [Q8CFT2-1]
    UniGeneiMm.250391.

    Genome annotation databases

    EnsembliENSMUST00000056053; ENSMUSP00000134686; ENSMUSG00000038384. [Q8CFT2-1]
    ENSMUST00000163030; ENSMUSP00000133933; ENSMUSG00000038384. [Q8CFT2-1]
    ENSMUST00000174836; ENSMUSP00000134461; ENSMUSG00000038384. [Q8CFT2-2]
    GeneIDi208043.
    KEGGimmu:208043.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC158114 Genomic DNA. No translation available.
    BC038367 mRNA. Translation: AAH38367.2 .
    BC040775 mRNA. Translation: AAH40775.1 .
    BC041681 mRNA. Translation: AAH41681.1 .
    AK122435 mRNA. Translation: BAC65717.1 .
    CCDSi CCDS59684.1. [Q8CFT2-1 ]
    RefSeqi NP_001035488.2. NM_001040398.2. [Q8CFT2-1 ]
    UniGenei Mm.250391.

    3D structure databases

    ProteinModelPortali Q8CFT2.
    SMRi Q8CFT2. Positions 97-202.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8CFT2.

    Proteomic databases

    PaxDbi Q8CFT2.
    PRIDEi Q8CFT2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000056053 ; ENSMUSP00000134686 ; ENSMUSG00000038384 . [Q8CFT2-1 ]
    ENSMUST00000163030 ; ENSMUSP00000133933 ; ENSMUSG00000038384 . [Q8CFT2-1 ]
    ENSMUST00000174836 ; ENSMUSP00000134461 ; ENSMUSG00000038384 . [Q8CFT2-2 ]
    GeneIDi 208043.
    KEGGi mmu:208043.

    Organism-specific databases

    CTDi 23067.
    MGIi MGI:2652820. Setd1b.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG2940.
    GeneTreei ENSGT00740000115089.
    HOVERGENi HBG055596.
    InParanoidi Q8CFT2.
    KOi K11422.
    OMAi QHWPPLP.
    OrthoDBi EOG7GQXTT.
    TreeFami TF106436.

    Miscellaneous databases

    PROi Q8CFT2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CFT2.
    Bgeei Q8CFT2.
    CleanExi MM_SETD1B.
    Genevestigatori Q8CFT2.

    Family and domain databases

    Gene3Di 3.30.70.330. 2 hits.
    InterProi IPR024657. COMPASS_Set1_N-SET.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR003616. Post-SET_dom.
    IPR000504. RRM_dom.
    IPR001214. SET_dom.
    [Graphical view ]
    Pfami PF11764. N-SET. 1 hit.
    PF00076. RRM_1. 1 hit.
    PF00856. SET. 1 hit.
    [Graphical view ]
    SMARTi SM00508. PostSET. 1 hit.
    SM00360. RRM. 1 hit.
    SM00317. SET. 1 hit.
    [Graphical view ]
    PROSITEi PS50868. POST_SET. 1 hit.
    PS50102. RRM. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 883-1985 (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Eye and Mammary tumor.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1090-1985 (ISOFORM 2).
    4. "Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex."
      Lee J.-H., Tate C.M., You J.-S., Skalnik D.G.
      J. Biol. Chem. 282:13419-13428(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiSET1B_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFT2
    Secondary accession number(s): Q80TK9, Q8CFQ8, Q8CGD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3