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Protein

Histone-lysine N-methyltransferase SETD1B

Gene

Setd1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Methyltransferase, RNA-binding, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase SETD1B (EC:2.1.1.43)
Alternative name(s):
SET domain-containing protein 1B
Gene namesi
Name:Setd1b
Synonyms:Kiaa1076, Set1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2652820 Setd1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003169941 – 1985Histone-lysine N-methyltransferase SETD1BAdd BLAST1985

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei977PhosphoserineBy similarity1
Modified residuei985PhosphoserineBy similarity1
Modified residuei1022PhosphoserineBy similarity1
Modified residuei1283PhosphoserineCombined sources1
Modified residuei1301PhosphoserineCombined sources1
Modified residuei1354PhosphoserineBy similarity1
Modified residuei1678PhosphoserineCombined sources1
Modified residuei1682PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CFT2
PeptideAtlasiQ8CFT2
PRIDEiQ8CFT2

PTM databases

iPTMnetiQ8CFT2
PhosphoSitePlusiQ8CFT2

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000038384
CleanExiMM_SETD1B
ExpressionAtlasiQ8CFT2 baseline and differential
GenevisibleiQ8CFT2 MM

Interactioni

Subunit structurei

Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with HCFC1. and ASH2L/ASH2. Interacts (via the RRM domain) with WDR82. Interacts (via the RRM domain) with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) only in the presence of WDR82. Binds specifically to CTD heptad repeats phosphorylated on 'Ser-5' of each heptad. Interacts with RBM15 (By similarity).By similarity

Protein-protein interaction databases

BioGridi228940, 2 interactors
STRINGi10090.ENSMUSP00000133933

Structurei

3D structure databases

ProteinModelPortaliQ8CFT2
SMRiQ8CFT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 180RRMPROSITE-ProRule annotationAdd BLAST89
Domaini1846 – 1963SETPROSITE-ProRule annotationAdd BLAST118
Domaini1969 – 1985Post-SETPROSITE-ProRule annotationAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 1690Pro-richAdd BLAST1318
Compositional biasi1031 – 1179Ser-richAdd BLAST149
Compositional biasi1059 – 1331Glu-richAdd BLAST273

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1080 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00760000119228
HOVERGENiHBG055596
InParanoidiQ8CFT2
KOiK11422
OMAiPVMQVDM
OrthoDBiEOG091G00IV
TreeFamiTF106436

Family and domain databases

CDDicd12549 RRM_Set1B, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR024657 COMPASS_Set1_N-SET
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR003616 Post-SET_dom
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034468 Set1B_RRM
IPR001214 SET_dom
IPR037842 SETD1B
PANTHERiPTHR22884:SF475 PTHR22884:SF475, 1 hit
PfamiView protein in Pfam
PF11764 N-SET, 1 hit
PF00076 RRM_1, 1 hit
PF00856 SET, 1 hit
SMARTiView protein in SMART
SM01291 N-SET, 1 hit
SM00508 PostSET, 1 hit
SM00360 RRM, 1 hit
SM00317 SET, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50868 POST_SET, 1 hit
PS50102 RRM, 1 hit
PS50280 SET, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CFT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENSHPHHHH QQPPPQPGPS GERRNHHWRS YKLMIDPALK KGHHKLYRYD
60 70 80 90 100
GQHFSLAMSS NRPVEIVEDP RVVGIWTKNK ELELSVPKFK IDEFYVGPVP
110 120 130 140 150
PKQVTFAKLN DNVRENFLRD MCKKYGEVEE VEILYNPKTK KHLGIAKVVF
160 170 180 190 200
ATVRGAKEAV QHLHSTSVMG NIIHVELDTK GETRMRFYEL LVTGRYTPQT
210 220 230 240 250
LPVGELDAIS PIVSETLQLS DALKRLKDGS LSAGCGSGSS SVTPNSGGTP
260 270 280 290 300
FSQDTAYSSC RLDTPNSYGQ GTPITPRLGT PFSQDSSYSS RQPTPSYLFS
310 320 330 340 350
QDPTATFKAR RHESKFTDAY NRRHEHHYVH NSAVAGATAP FRGSSDLSFG
360 370 380 390 400
TVGSSGTPFK AQSQDATTFA HTPPPAQTAT ASGFKSAFSP YQTPAPPFPP
410 420 430 440 450
PPEEPTATAA FGSRDSGEFR RAPAPPPLPP AEPPAKEKPG TPPGPPPPDS
460 470 480 490 500
NSMELGGRPT FGWSPEPCDS PGTPTLESSP AGPEKPHDSL DSRIEMLLKE
510 520 530 540 550
QRTKLPFLRE QDSDTEIQME GSPISSSSSQ LSPLSHFGTN SQPGFRGPSP
560 570 580 590 600
PSSRPSSTGL EDISPTPLPD SDEDEDLGLG LGPRPPPEPG PPDPMGLLGQ
610 620 630 640 650
TAEVDLDLAG DRTPTSERMD EGQQSSGEDM EISDDEMPSA PITSADCPKP
660 670 680 690 700
MVVTPGAGAV AAPNVLAPNL PLPPPPGFPP LPPPPPPPPP QPGFPMPPPL
710 720 730 740 750
PPPPPPPPPA HPAVTVPPPP LPAPPGVPPP PILPPLPPFP PGLFPVMQVD
760 770 780 790 800
MSHVLGGQWG GMPMSFQMQT QMLSRLMTGQ GACPYPPFMA AAAAAASAGL
810 820 830 840 850
QFVNLPPYRS PFSLSNSGPG RGQHWPPLPK FDPSVPPPGY IPRQEDPHKA
860 870 880 890 900
TVDGVLLVVL KELKAIMKRD LNRKMVEVVA FRAFDEWWDK KERMAKASLT
910 920 930 940 950
PVKSGEHKDE DRPKPKDRIA SCLLESWGKG EGLGYEGLGL GIGLRGAIRL
960 970 980 990 1000
PSFKVKRKEP PDTASSGDQK RLRPSTSVDE EDEESERERD RDIADAPCEL
1010 1020 1030 1040 1050
TKRDPKSVGV RRRPGRPLEL DSGGEEDEKE SLSASSSSSA SSSSGSSTTS
1060 1070 1080 1090 1100
PSSSASDKEE EDRESTEEEE EEEEEEAEEE EEEGPRSRIS SPSSSSSSDK
1110 1120 1130 1140 1150
DDEDDNEADS DGQIDSDIDD QGAPLSEASE KDNGDSEEEE TESITTSKAP
1160 1170 1180 1190 1200
AESSSSSSES SGSSEFESSS ESESSSSSSE DEEEMTVPGV EEEEEEEEEE
1210 1220 1230 1240 1250
EKETAMAAAT VVAMAEESMP PAGGQDFEQD RAEVPLGPRG PMRESLGTEE
1260 1270 1280 1290 1300
EVDIEAEDEV PEMQAPELEE PPLPMGARKL EGSPEPPEEP GPNTQGDMLL
1310 1320 1330 1340 1350
SPELPARETE EAQLPSPPEH GPESDLDMEP EPPPMLSLPL QPPLPPPRLL
1360 1370 1380 1390 1400
RPPSPPPEPE TPEPPKPPVP LEPPPEDHPP RTPGLCGSLA KSQSTETVPA
1410 1420 1430 1440 1450
TPGGEPPLSG SSSGLSLSSP QVPGSPFSYP SPSPGLSSGG LPRTPGRDFS
1460 1470 1480 1490 1500
FTPTFPEPSG PLLLPVCPLP TGRRDERTGP LASPVLLETG LPLPLPLPLP
1510 1520 1530 1540 1550
LPLALPVPVL RAQPRPPPQL PPLLPATLAP CPTPIKRKPG RPRRSPPSML
1560 1570 1580 1590 1600
SLDGPLVRPP PGPALGRDLL LLPGQPPAPI FPSAHDPRAV TLDFRNTGIP
1610 1620 1630 1640 1650
APPPPLPPQP PPPPPPPPVE STKLPFKELD NQWPSEAIPP GPRRDEVTEE
1660 1670 1680 1690 1700
YVDLAKVRGP WRRPPKKRHE DLVAPSASPE PSPPQPLFRP RSEFEEMTIL
1710 1720 1730 1740 1750
YDIWNGGIDE EDIRFLCVTY ERLLQQDNGM DWLNDTLWVY HPSTSLSSAK
1760 1770 1780 1790 1800
KKKREDGIRE HVTGCARSEG FYTIDKKDKL RYLNSSRAST DEPPMDTQGM
1810 1820 1830 1840 1850
SIPAQPHAST RAGSERRSEQ RRLLSSFTGS CDSDLLKFNQ LKFRKKKLKF
1860 1870 1880 1890 1900
CKSHIHDWGL FAMEPIAADE MVIEYVGQNI RQVIADMREK RYEDEGIGSS
1910 1920 1930 1940 1950
YMFRVDHDTI IDATKCGNFA RFINHSCNPN CYAKVITVES QKKIVIYSKQ
1960 1970 1980
HINVNEEITY DYKFPIEDVK IPCLCGSENC RGTLN
Length:1,985
Mass (Da):215,352
Last modified:February 5, 2008 - v2
Checksum:i760EA261769292EC
GO
Isoform 2 (identifier: Q8CFT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1139-1179: Missing.

Note: No experimental confirmation available.
Show »
Length:1,944
Mass (Da):211,286
Checksum:iE2BD1B70C876448C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1222A → V in BAC65717 (PubMed:12693553).Curated1
Sequence conflicti1411S → G in BAC65717 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0308511139 – 1179Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC158114 Genomic DNA No translation available.
BC038367 mRNA Translation: AAH38367.2
BC040775 mRNA Translation: AAH40775.1
BC041681 mRNA Translation: AAH41681.1
AK122435 mRNA Translation: BAC65717.1
CCDSiCCDS59684.1 [Q8CFT2-1]
RefSeqiNP_001035488.2, NM_001040398.2 [Q8CFT2-1]
UniGeneiMm.250391

Genome annotation databases

EnsembliENSMUST00000056053; ENSMUSP00000134686; ENSMUSG00000038384 [Q8CFT2-1]
ENSMUST00000163030; ENSMUSP00000133933; ENSMUSG00000038384 [Q8CFT2-1]
ENSMUST00000174836; ENSMUSP00000134461; ENSMUSG00000038384 [Q8CFT2-2]
GeneIDi208043
KEGGimmu:208043
UCSCiuc008zng.2 mouse [Q8CFT2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSET1B_MOUSE
AccessioniPrimary (citable) accession number: Q8CFT2
Secondary accession number(s): Q80TK9, Q8CFQ8, Q8CGD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health