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Protein

DnaJ homolog subfamily C member 27

Gene

Dnajc27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 30GTPBy similarity8
Nucleotide bindingi71 – 75GTPBy similarity5
Nucleotide bindingi134 – 137GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of MAPK cascade Source: UniProtKB
  • regulation of MAPK export from nucleus Source: UniProtKB

Keywordsi

LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 27
Alternative name(s):
Rab and DnaJ domain-containing protein
Gene namesi
Name:Dnajc27
Synonyms:Rabj, Rbj
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2443036. Dnajc27.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30S → N: Abolishes GTP binding. 1 Publication1
Mutagenesisi75H → L: Abolishes GTPase activity. 1 Publication1
Mutagenesisi75H → Q: Increases GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003329761 – 273DnaJ homolog subfamily C member 27Add BLAST273

Proteomic databases

MaxQBiQ8CFP6.
PaxDbiQ8CFP6.
PeptideAtlasiQ8CFP6.
PRIDEiQ8CFP6.

PTM databases

iPTMnetiQ8CFP6.
PhosphoSitePlusiQ8CFP6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020657.
GenevisibleiQ8CFP6. MM.

Interactioni

Subunit structurei

Interacts directly with MAPK1 (wild-type and kinase-deficient forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type and kinase-deficient forms).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Map2k1P319383EBI-9548773,EBI-298860

Protein-protein interaction databases

IntActiQ8CFP6. 2 interactors.
STRINGi10090.ENSMUSP00000020986.

Structurei

3D structure databases

ProteinModelPortaliQ8CFP6.
SMRiQ8CFP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini217 – 273JPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 18Required for interaction with MAPK11 PublicationAdd BLAST18

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0098. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133872.
HOGENOMiHOG000233969.
HOVERGENiHBG056504.
InParanoidiQ8CFP6.
KOiK19372.
OMAiFYVSIVD.
OrthoDBiEOG091G0IXD.
PhylomeDBiQ8CFP6.
TreeFamiTF328564.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF00071. Ras. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS50076. DNAJ_2. 1 hit.
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CFP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METNVPKRKE PAKSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG
60 70 80 90 100
IDYGVTKVQV RDREIKVNIF DMAGHPFFFE VRNEFYKDTQ GVILVYDVGQ
110 120 130 140 150
KDSFDALDSW LAEMKQELGP HGNMDNIVFV VCANKIDCSK HRCIDESEGR
160 170 180 190 200
LWAESKGFLY FETSAQTGEG INEMFQTFYL SIVDLCENGG KRPTASSSAS
210 220 230 240 250
FTKEQADTIR RIRNSKDSWE MLGVRPGASR EEVNKAYRKL AVLLHPDKCV
260 270
APGSEDAFKA VVNARTALLK NIK
Length:273
Mass (Da):30,831
Last modified:March 1, 2003 - v1
Checksum:i805523789CECC329
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3 – 5TNV → AYM in AAK62027 (Ref. 1) Curated3
Sequence conflicti34K → E in AAK62027 (Ref. 1) Curated1
Sequence conflicti43S → F in AAK62027 (Ref. 1) Curated1
Sequence conflicti57K → E in AAK62027 (Ref. 1) Curated1
Sequence conflicti208T → A in BAC33667 (PubMed:16141072).Curated1
Sequence conflicti273K → KW in AAK62027 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035893 mRNA. Translation: AAK62027.1.
AK049300 mRNA. Translation: BAC33667.1.
BC042643 mRNA. Translation: AAH42643.1.
BC051140 mRNA. Translation: AAH51140.1.
BK001285 mRNA. Translation: DAA01324.1.
CCDSiCCDS25786.1.
RefSeqiNP_694722.2. NM_153082.4.
UniGeneiMm.271656.

Genome annotation databases

EnsembliENSMUST00000020986; ENSMUSP00000020986; ENSMUSG00000020657.
ENSMUST00000049584; ENSMUSP00000106803; ENSMUSG00000020657.
ENSMUST00000219923; ENSMUSP00000151848; ENSMUSG00000020657.
GeneIDi217378.
KEGGimmu:217378.
UCSCiuc007mxi.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDJC27_MOUSE
AccessioniPrimary (citable) accession number: Q8CFP6
Secondary accession number(s): Q8BX00, Q923I0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2003
Last modified: June 7, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families