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Reviewed, UniProtKB/Swiss-Prot Q8CFN2 (CDC42_RAT)

Last modified February 9, 2010. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cell division control protein 42 homolog
Gene names
Name: Cdc42
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length191 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia By similarity.

Enzyme regulation

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase By similarity.

Subunit structure

Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1 By similarity.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence similarities

Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CFN2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CFN2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-191: PKKSRRCVLL → TQPKRKCCIF

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Cell division control protein 42 homolog
PRO_0000270829
Propeptide189 – 1913Removed in mature form By similarity
PRO_0000270830

Regions

Nucleotide binding10 – 178GTP By similarity
Nucleotide binding57 – 615GTP By similarity
Nucleotide binding115 – 1184GTP By similarity
Motif32 – 409Effector region Potential

Amino acid modifications

Modified residue1351N6-acetyllysine By similarity
Modified residue1441N6-acetyllysine By similarity
Modified residue1881Cysteine methyl ester By similarity
Lipidation1881S-geranylgeranyl cysteine By similarity

Natural variations

Alternative sequence182 – 19110PKKSRRCVLL → TQPKRKCCIF in isoform 2.
VSP_022241

Experimental info

Sequence conflict1591A → P in AAF15538. Ref.1
Sequence conflict1591A → P in AAN63806. Ref.2
Sequence conflict1631K → R in AAF15538. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 51A437E22A4D8FFF

FASTA19121,259
        10         20         30         40         50         60 
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 

        70         80         90        100        110        120 
QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR 

       130        140        150        160        170        180 
DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP 

       190 
EPKKSRRCVL L

« Hide

Isoform 2.

Checksum: 34B44F923D7D996B
Show »

FASTA19121,283

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References

« Hide 'large scale' references
[1]"Molecular cloning and sequencing of rat Cdc42 GTPase cDNA."
Han J.-S., Kim J.-H., Kim J.G., Park J.-B., Noh D.-Y., Lee K.-H.
Exp. Mol. Med. 32:115-119(2000) [PubMed: 11048641] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The cdc42 plays a protective role in the brain ischemia."
Chen D., Manabu M., Lan J., Jin J., Simon R.P.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Hippocampus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Pituitary.
[4]Lubec G., Afjehi-Sadat L., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 6-16; 108-120 AND 167-183, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain and Spinal cord.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF205635 mRNA. Translation: AAF15538.1.
AF491841 mRNA. Translation: AAN63806.1.
BC060535 mRNA. Translation: AAH60535.1.
IPIIPI00285606.
IPI00388289.
RefSeqNP_741991.3.
UniGeneRn.60067

3D structure databases

HSSPHSSP built from PDB template 1AM4 based on UniProtKB P60953.
SMRQ8CFN2. Positions 1-191.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8CFN2.

PTM databases

PhosphoSiteQ8CFN2.

Proteomic databases

PRIDEQ8CFN2.

Genome annotation databases

EnsemblENSRNOT00000029025; ENSRNOP00000030928; ENSRNOG00000013536; Rattus norvegicus. [Genome view]
GeneID64465.
KEGGrno:64465.

Organism-specific databases

CTD64465.
RGD71043. Cdc42.

Phylogenomic databases

eggNOGroNOG07751.
HOVERGENQ8CFN2.
InParanoidQ8CFN2.
OMAEITHHCQ.
OrthoDBEOG9KH5FB.
PhylomeDBQ8CFN2.

Gene expression databases

ArrayExpressQ8CFN2.
GenevestigatorQ8CFN2.

Family and domain databases

InterProIPR003578. GTPase_Rho.
IPR013753. Ras.
IPR001806. Ras_GTPase.
IPR005225. Small_GTP_bd.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00174. RHO. 1 hit.
[Graphical view]
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio613224.

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Entry information

Entry nameCDC42_RAT
AccessionPrimary (citable) accession number: Q8CFN2
Secondary accession number(s): Q6P9Y3, Q71TW5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: February 9, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents