Q8CFN2 (CDC42_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division control protein 42 homolog

Gene names

Name:

Cdc42

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration By similarity.
Enzyme regulation	Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase By similarity.
Subunit structure	Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1 By similarity. Interacts with USP6 By similarity. Interacts with NEK6 By similarity. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Midbody By similarity. Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Prenylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	establishment of cell polarity Traceable author statement. Source: RGD positive regulation of DNA replication Inferred from mutant phenotype. Source: RGD positive regulation of JNK cascade Inferred from direct assay. Source: RGD positive regulation of cell cycle cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB regulation of attachment of spindle microtubules to kinetochore Inferred from sequence or structural similarity. Source: UniProtKB regulation of exocytosis Traceable author statement. Source: UniProtKB regulation of filopodium assembly Inferred from sequence or structural similarity. Source: UniProtKB response to glucose stimulus Traceable author statement. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from direct assay. Source: RGD microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from sequence or structural similarity. Source: UniProtKB mitotic spindle Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from direct assay. Source: UniProtKB secretory granule Inferred from direct assay. Source: UniProtKB spindle midzone Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GTP binding Traceable author statement. Source: UniProtKB GTPase activity Traceable author statement. Source: RGD mitogen-activated protein kinase kinase kinase binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CFN2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CFN2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-191: PKKSRRCVLL → TQPKRKCCIF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Cell division control protein 42 homolog		PRO_0000270829
	Propeptide	189 – 191	3	Removed in mature form By similarity		PRO_0000270830
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	115 – 118	4	GTP By similarity
	Motif	32 – 40	9	Effector region Potential
Amino acid modifications
	Modified residue	135	1	N6-acetyllysine By similarity
	Modified residue	144	1	N6-acetyllysine By similarity
	Modified residue	188	1	Cysteine methyl ester By similarity
	Lipidation	188	1	S-geranylgeranyl cysteine By similarity
Natural variations
	Alternative sequence	182 – 191	10	PKKSRRCVLL → TQPKRKCCIF in isoform 2.		VSP_022241
Experimental info
	Sequence conflict	159	1	A → P in AAF15538. Ref.1
	Sequence conflict	159	1	A → P in AAN63806. Ref.2
	Sequence conflict	163	1	K → R in AAF15538. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 51A437E22A4D8FFF	FASTA	191	21,259
	10 20 30 40 50 60 MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 70 80 90 100 110 120 QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR 130 140 150 160 170 180 DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP 190 EPKKSRRCVL L
	Isoform 2 [UniParc]. Checksum: 34B44F923D7D996B	FASTA	191	21,283
	10 20 30 40 50 60 MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 70 80 90 100 110 120 QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR 130 140 150 160 170 180 DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP 190 ETQPKRKCCI F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequencing of rat Cdc42 GTPase cDNA." Han J.-S., Kim J.-H., Kim J.G., Park J.-B., Noh D.-Y., Lee K.-H. Exp. Mol. Med. 32:115-119(2000) [PubMed: 11048641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"The cdc42 plays a protective role in the brain ischemia." Chen D., Manabu M., Lan J., Jin J., Simon R.P. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hippocampus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pituitary.
[4]	Lubec G., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 6-16; 108-120 AND 167-183, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF205635 mRNA. Translation: AAF15538.1. AF491841 mRNA. Translation: AAN63806.1. BC060535 mRNA. Translation: AAH60535.1.
IPI	IPI00285606. IPI00388289.
RefSeq	NP_741991.3. NM_171994.4.
UniGene	Rn.60067.
3D structure databases
HSSP	HSSP built from PDB template 1AM4 based on UniProtKB P60953.
ProteinModelPortal	Q8CFN2.
SMR	Q8CFN2. Positions 1-191.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3381856.
STRING	Q8CFN2.
PTM databases
PhosphoSite	Q8CFN2.
Proteomic databases
PRIDE	Q8CFN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018118; ENSRNOP00000018118; ENSRNOG00000013536. ENSRNOT00000029025; ENSRNOP00000030928; ENSRNOG00000013536.
GeneID	64465.
KEGG	rno:64465.
Organism-specific databases
CTD	998.
RGD	71043. Cdc42.
Phylogenomic databases
eggNOG	roNOG07751.
GeneTree	ENSGT00560000076570.
HOVERGEN	HBG009351.
InParanoid	Q8CFN2.
OMA	EITHHCQ.
OrthoDB	EOG4R23VV.
PhylomeDB	Q8CFN2.
Gene expression databases
ArrayExpress	Q8CFN2.
Genevestigator	Q8CFN2.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
KO	K04393.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613224.

Entry information

Entry name

CDC42_RAT

Accession

Primary (citable) accession number: Q8CFN2
Secondary accession number(s): Q6P9Y3, Q71TW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	November 16, 2011
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program