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Protein

Transcription factor IIIB 90 kDa subunit

Gene

Brf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi6 – 61ZincBy similarity
Metal bindingi9 – 91ZincBy similarity
Metal bindingi25 – 251ZincBy similarity
Metal bindingi28 – 281ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2 – 3332TFIIB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 90 kDa subunit
Short name:
TFIIIB90
Short name:
mTFIIIB90
Alternative name(s):
B-related factor 1
Short name:
BRF-1
Gene namesi
Name:Brf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919558. Brf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 676676Transcription factor IIIB 90 kDa subunitPRO_0000119348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei365 – 3651PhosphothreonineCombined sources
Modified residuei449 – 4491PhosphoserineCombined sources
Modified residuei552 – 5521PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CFK2.
MaxQBiQ8CFK2.
PaxDbiQ8CFK2.
PRIDEiQ8CFK2.

PTM databases

iPTMnetiQ8CFK2.
PhosphoSiteiQ8CFK2.

Interactioni

Subunit structurei

TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000011302.

Structurei

3D structure databases

ProteinModelPortaliQ8CFK2.
SMRiQ8CFK2. Positions 93-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati91 – 172821Add
BLAST
Repeati185 – 269852Add
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2 – 3332TFIIB-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1598. Eukaryota.
COG1405. LUCA.
HOGENOMiHOG000198688.
HOVERGENiHBG023540.
InParanoidiQ8CFK2.
PhylomeDBiQ8CFK2.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PTHR11618:SF4. PTHR11618:SF4. 1 hit.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CFK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGRVCRGCG GTDIELDAAR GDAVCTGCGS VLEDNIIVSE VQFVENSGGG
60 70 80 90 100
SSAVGQFVSL DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGSQL
110 120 130 140 150
QLNQHCLDTA FNFFKMAVSK HLTRGRKMAH VIAACLYLVC RTEGTPHMLL
160 170 180 190 200
DLSDLLQVNV YVLGKTFLLL ARELCINAPA IDPCLYIPRF AHLLEFGEKN
210 220 230 240 250
HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH DFRRTVKEVI
260 270 280 290 300
SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK
310 320 330 340 350
LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GALANLSKDG
360 370 380 390 400
SGEDATSSPR CEEDTEDEEL EAAASHMNKD FYRELLGDDD GSEAAGDPDG
410 420 430 440 450
GSRPLALESL LGPLPTAASL GISDSIRECI SSPSGDPKDS SGDGELDLSG
460 470 480 490 500
IDDLEIDRYI LNESEARVKA ELWMRENAEY LREQKEKEAR IAKEKELGIY
510 520 530 540 550
KEHKPKKSCK RREPILASTA GEAIEKMLEQ KKISSKINYS VLRDLNSKGG
560 570 580 590 600
GSPPRDDSQP PERASTKKLS RRKRATTRNS ADPGTSTGKR LRPLLSAQPA
610 620 630 640 650
KKAAVGEALL LSSPALGAEP VKPSAVLVES GPVPYHPEED ADEEDAEDED
660 670
GEPCVSALQM MGGNDYGCDG DEDDGY
Length:676
Mass (Da):73,799
Last modified:March 1, 2003 - v1
Checksum:i35110297F64E7E7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC037224 mRNA. Translation: AAH37224.1.
CCDSiCCDS49195.1.
UniGeneiMm.196721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC037224 mRNA. Translation: AAH37224.1.
CCDSiCCDS49195.1.
UniGeneiMm.196721.

3D structure databases

ProteinModelPortaliQ8CFK2.
SMRiQ8CFK2. Positions 93-267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000011302.

PTM databases

iPTMnetiQ8CFK2.
PhosphoSiteiQ8CFK2.

Proteomic databases

EPDiQ8CFK2.
MaxQBiQ8CFK2.
PaxDbiQ8CFK2.
PRIDEiQ8CFK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1919558. Brf1.

Phylogenomic databases

eggNOGiKOG1598. Eukaryota.
COG1405. LUCA.
HOGENOMiHOG000198688.
HOVERGENiHBG023540.
InParanoidiQ8CFK2.
PhylomeDBiQ8CFK2.

Miscellaneous databases

PROiQ8CFK2.
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PTHR11618:SF4. PTHR11618:SF4. 1 hit.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF3B_MOUSE
AccessioniPrimary (citable) accession number: Q8CFK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 1, 2003
Last modified: March 16, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.