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Q8CFI7

- RPB2_MOUSE

UniProt

Q8CFI7 - RPB2_MOUSE

Protein

DNA-directed RNA polymerase II subunit RPB2

Gene

Polr2b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi792 – 7921Magnesium; shared with RPB1By similarity
    Metal bindingi1119 – 11191ZincBy similarity
    Metal bindingi1122 – 11221ZincBy similarity
    Metal bindingi1137 – 11371ZincBy similarity
    Metal bindingi1140 – 11401ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1119 – 114022C4-typeAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: InterPro
    3. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. ribonucleoside binding Source: InterPro

    GO - Biological processi

    1. transcription from RNA polymerase II promoter Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_203462. Formation of the Early Elongation Complex.
    REACT_213655. Transcription-coupled NER (TC-NER).
    REACT_221178. MicroRNA (miRNA) biogenesis.
    REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB2 (EC:2.7.7.6)
    Alternative name(s):
    DNA-directed RNA polymerase II 140 kDa polypeptide
    DNA-directed RNA polymerase II subunit B
    RNA polymerase II subunit 2
    RNA polymerase II subunit B2
    Gene namesi
    Name:Polr2b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2388280. Polr2b.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11741174DNA-directed RNA polymerase II subunit RPB2PRO_0000048086Add
    BLAST

    Proteomic databases

    MaxQBiQ8CFI7.
    PaxDbiQ8CFI7.
    PRIDEiQ8CFI7.

    PTM databases

    PhosphoSiteiQ8CFI7.

    Expressioni

    Gene expression databases

    BgeeiQ8CFI7.
    CleanExiMM_POLR2B.
    GenevestigatoriQ8CFI7.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with WDR82 By similarity. Interacts with MEN1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi231111. 8 interactions.
    DIPiDIP-57023N.
    IntActiQ8CFI7. 8 interactions.
    MINTiMINT-4133069.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFI7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA polymerase beta chain family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1119 – 114022C4-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0085.
    GeneTreeiENSGT00750000117589.
    HOGENOMiHOG000222962.
    HOVERGENiHBG017744.
    InParanoidiQ8CFI7.
    KOiK03010.
    OMAiDSQMLEM.
    OrthoDBiEOG7JT6VC.
    PhylomeDBiQ8CFI7.
    TreeFamiTF103037.

    Family and domain databases

    Gene3Di2.40.270.10. 2 hits.
    2.40.50.150. 1 hit.
    3.90.1110.10. 1 hit.
    InterProiIPR015712. DNA-dir_RNA_pol_su2.
    IPR007120. DNA-dir_RNA_pol_su2_6.
    IPR007121. RNA_pol_bsu_CS.
    IPR007644. RNA_pol_bsu_protrusion.
    IPR007642. RNA_pol_Rpb2_2.
    IPR007645. RNA_pol_Rpb2_3.
    IPR007646. RNA_pol_Rpb2_4.
    IPR007647. RNA_pol_Rpb2_5.
    IPR007641. RNA_pol_Rpb2_7.
    IPR014724. RNA_pol_RPB2_OB-fold.
    [Graphical view]
    PANTHERiPTHR20856. PTHR20856. 1 hit.
    PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
    PF04561. RNA_pol_Rpb2_2. 1 hit.
    PF04565. RNA_pol_Rpb2_3. 1 hit.
    PF04566. RNA_pol_Rpb2_4. 1 hit.
    PF04567. RNA_pol_Rpb2_5. 1 hit.
    PF00562. RNA_pol_Rpb2_6. 1 hit.
    PF04560. RNA_pol_Rpb2_7. 1 hit.
    [Graphical view]
    PROSITEiPS01166. RNA_POL_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CFI7-1 [UniParc]FASTAAdd to Basket

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    MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF     50
    IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFEQ IYLSKPTHWE 100
    RDGAPSPMMP NEARLRNLTY SAPLYVDITK TVIKEGEEQL QTQHQKTFIG 150
    KIPIMLRSTY CLLNGLTDRD LCELNECPLD PGGYFIINGS EKVLIAQEKM 200
    ATNTVYVFAK KDSKYAYTGE CRSCLENSSR PTSTIWVSML ARGGQGAKKS 250
    AIGQRIVATL PYIKQEVPII IVFRALGFVS DRDILEHIIY DFEDPEMMEM 300
    VKPSLDEAFV IQEQNVALNF IGSRGAKPGV TKEKRIKYAK EVLQKEMLPH 350
    VGVSDFCETK KAYFLGYMVH RLLLAALGRR ELDDRDHYGN KRLDLAGPLL 400
    AFLFRGMFKN LLKEVRIYAQ KFIDRGKDFN LELAIKTRII SDGLKYSLAT 450
    GNWGDQKKAH QARAGVSQVL NRLTFASTLS HLRRLNSPIG RDGKLAKPRQ 500
    LHNTLWGMVC PAETPEGHAV GLVKNLALMA YISVGSQPSP ILEFLEEWSM 550
    ENLEEISPAA IADATKIFVN GCWVGIHKDP EQLMNTLRKL RRQMDIIVSE 600
    VSMIRDIRER EIRIYTDAGR ICRPLLIVEK QKLLLKKRHI DQLKEREYNN 650
    YSWQDLVASG VVEYIDTLEE ETVMLAMTPD DLQEKEVAYC STYTHCEIHP 700
    SMILGVCASI IPFPDHNQSP RNTYQSAMGK QAMGVYITNF HVRMDTLAHV 750
    LYYPQKPLVT TRSMEYLRFR ELPAGINSIV AIASYTGYNQ EDSVIMNRSA 800
    VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYEKLDD 850
    DGLIAPGVRV SGDDVIIGKT VTLPENEDEL ESTNRRYTKR DCSTFLRTSE 900
    TGIVDQVMVT LNQEGYKFCK IRVRSVRIPQ IGDKFASRHG QKGTCGIQYR 950
    QEDMPFTCEG ITPDIIINPH AIPSRMTIGH LIECLQGKVS ANKGEIGDAT 1000
    PFNDAVNVQK ISNLLSDYGY HLRGNEVLYN GFTGRKITSQ IFIGPTYYQR 1050
    LKHMVDDKIH SRARGPIQIL NRQPMEGRSR DGGLRFGEME RDCQIAHGAA 1100
    QFLRERLFEA SDPYQVHVCN LCGIMAIANT RTHTYECRGC RNKTQISLVR 1150
    MPYACKLLFQ ELMSMSIAPR MMSV 1174
    Length:1,174
    Mass (Da):133,911
    Last modified:August 31, 2004 - v2
    Checksum:iF941FA6EC03E2160
    GO

    Sequence cautioni

    The sequence AAH38472.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC038472 mRNA. Translation: AAH38472.1. Different initiation.
    CCDSiCCDS51528.1.
    RefSeqiNP_722493.2. NM_153798.2.
    UniGeneiMm.273217.

    Genome annotation databases

    EnsembliENSMUST00000031167; ENSMUSP00000031167; ENSMUSG00000029250.
    GeneIDi231329.
    KEGGimmu:231329.
    UCSCiuc008xwe.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC038472 mRNA. Translation: AAH38472.1 . Different initiation.
    CCDSi CCDS51528.1.
    RefSeqi NP_722493.2. NM_153798.2.
    UniGenei Mm.273217.

    3D structure databases

    ProteinModelPortali Q8CFI7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231111. 8 interactions.
    DIPi DIP-57023N.
    IntActi Q8CFI7. 8 interactions.
    MINTi MINT-4133069.

    PTM databases

    PhosphoSitei Q8CFI7.

    Proteomic databases

    MaxQBi Q8CFI7.
    PaxDbi Q8CFI7.
    PRIDEi Q8CFI7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031167 ; ENSMUSP00000031167 ; ENSMUSG00000029250 .
    GeneIDi 231329.
    KEGGi mmu:231329.
    UCSCi uc008xwe.2. mouse.

    Organism-specific databases

    CTDi 5431.
    MGIi MGI:2388280. Polr2b.

    Phylogenomic databases

    eggNOGi COG0085.
    GeneTreei ENSGT00750000117589.
    HOGENOMi HOG000222962.
    HOVERGENi HBG017744.
    InParanoidi Q8CFI7.
    KOi K03010.
    OMAi DSQMLEM.
    OrthoDBi EOG7JT6VC.
    PhylomeDBi Q8CFI7.
    TreeFami TF103037.

    Enzyme and pathway databases

    Reactomei REACT_203462. Formation of the Early Elongation Complex.
    REACT_213655. Transcription-coupled NER (TC-NER).
    REACT_221178. MicroRNA (miRNA) biogenesis.
    REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Miscellaneous databases

    ChiTaRSi POLR2B. mouse.
    NextBioi 380519.
    PROi Q8CFI7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CFI7.
    CleanExi MM_POLR2B.
    Genevestigatori Q8CFI7.

    Family and domain databases

    Gene3Di 2.40.270.10. 2 hits.
    2.40.50.150. 1 hit.
    3.90.1110.10. 1 hit.
    InterProi IPR015712. DNA-dir_RNA_pol_su2.
    IPR007120. DNA-dir_RNA_pol_su2_6.
    IPR007121. RNA_pol_bsu_CS.
    IPR007644. RNA_pol_bsu_protrusion.
    IPR007642. RNA_pol_Rpb2_2.
    IPR007645. RNA_pol_Rpb2_3.
    IPR007646. RNA_pol_Rpb2_4.
    IPR007647. RNA_pol_Rpb2_5.
    IPR007641. RNA_pol_Rpb2_7.
    IPR014724. RNA_pol_RPB2_OB-fold.
    [Graphical view ]
    PANTHERi PTHR20856. PTHR20856. 1 hit.
    Pfami PF04563. RNA_pol_Rpb2_1. 1 hit.
    PF04561. RNA_pol_Rpb2_2. 1 hit.
    PF04565. RNA_pol_Rpb2_3. 1 hit.
    PF04566. RNA_pol_Rpb2_4. 1 hit.
    PF04567. RNA_pol_Rpb2_5. 1 hit.
    PF00562. RNA_pol_Rpb2_6. 1 hit.
    PF04560. RNA_pol_Rpb2_7. 1 hit.
    [Graphical view ]
    PROSITEi PS01166. RNA_POL_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiRPB2_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3