Q8CFI5 (SYPM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable proline--tRNA ligase, mitochondrial
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:

Pars2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
Subcellular location	Mitochondrion matrix Potential.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 475	446	Probable proline--tRNA ligase, mitochondrial		PRO_0000035819
Experimental info
	Sequence conflict	240	1	Q → R in AAH38817. Ref.2
	Sequence conflict	314	1	S → G in AAH38817. Ref.2
	Sequence conflict	397	1	I → V in AAH38817. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8CFI5 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 184D4DCA2B226549	FASTA	475	53,518
	10 20 30 40 50 60 MEGLLTRCRT LSALAACSLR HCRYIIHKCY HRAPGRGQRL VLSRMFQPQN LREDQVLSLE 70 80 90 100 110 120 GRASDLTCKS QRLMLQVGLI LPASPGCYHL MPYTVRAVEK LVRVIDQEMQ AIGGQKINMP 130 140 150 160 170 180 SLSPAELWRA TSRWDLMGRE LLRLRDRHGK EYCLGPTHEE AVTALVASQK KLSYKQLPLL 190 200 210 220 230 240 LYQVTRKFRD EPRPRFGLLR GREFYMKDMY TFDSSSEAAQ ETYGLVCDAY CRLFDRLGLQ 250 260 270 280 290 300 WMKARADVGS IGGTMSHEFQ LPVDIGEDRL VVCPSCHFSA NTEILDLSQK ICPDCQGPLT 310 320 330 340 350 360 ETKGIEVGHT FYLSTKYSSI FNALFTNAHG ESLLAEMGCY GLGVTRILAA AIEVLSTEDC 370 380 390 400 410 420 IRWPRLLAPY QVCVIPPKKG SKETAATEIV EKLYDDIMEA VPQLRGEVLL DDRTHLTIGN 430 440 450 460 470 RLKDANKLGY PFVIIAGKRA LEDPAHFEVW SQNTGEVVFL TKEGVMELLT GVHVV

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NMRI. Tissue: Eye and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL929585 Genomic DNA. Translation: CAM27480.1. BC027073 mRNA. Translation: AAH27073.1. BC038817 mRNA. Translation: AAH38817.1.
IPI	IPI00229312.
RefSeq	NP_758476.2. NM_172272.2.
UniGene	Mm.134029.
3D structure databases
ProteinModelPortal	Q8CFI5.
SMR	Q8CFI5. Positions 37-470.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CFI5.
Proteomic databases
PRIDE	Q8CFI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000058905; ENSMUSP00000053160; ENSMUSG00000043572.
GeneID	230577.
KEGG	mmu:230577.
NMPDR	fig|10090.3.peg.10014.
Organism-specific databases
CTD	25973.
MGI	MGI:2386296. Pars2.
Phylogenomic databases
eggNOG	roNOG15418.
GeneTree	ENSGT00390000010922.
HOVERGEN	HBG056542.
InParanoid	Q8CFI5.
OrthoDB	EOG466VKW.
PhylomeDB	Q8CFI5.
Gene expression databases
ArrayExpress	Q8CFI5.
Bgee	Q8CFI5.
CleanEx	MM_PARS2.
Genevestigator	Q8CFI5.
GermOnline	ENSMUSG00000043572. Mus musculus.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

SYPM_MOUSE

Accession

Primary (citable) accession number: Q8CFI5
Secondary accession number(s): A2AVQ8, Q8R0C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program