Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 R1

Gene

Cdc34

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity

Enzyme regulationi

CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-202424 Downstream TCR signaling
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 R1 (EC:2.3.2.23)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme R1 (EC:2.3.2.24)
E2 ubiquitin-conjugating enzyme R1
Ubiquitin-conjugating enzyme E2-32 kDa complementing
Ubiquitin-conjugating enzyme E2-CDC34
Ubiquitin-protein ligase R1
Gene namesi
Name:Cdc34
Synonyms:Ubch3, Ube2r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:102657 Cdc34

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824521 – 235Ubiquitin-conjugating enzyme E2 R1Add BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203Phosphoserine; by CK2By similarity1
Modified residuei221Phosphoserine; by CK2By similarity1
Modified residuei230Phosphoserine; by CK2By similarity1
Modified residuei232Phosphothreonine; by CK2By similarity1
Modified residuei235Phosphoserine; by CK2By similarity1

Post-translational modificationi

Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CFI2
MaxQBiQ8CFI2
PaxDbiQ8CFI2
PeptideAtlasiQ8CFI2
PRIDEiQ8CFI2

PTM databases

iPTMnetiQ8CFI2
PhosphoSitePlusiQ8CFI2

Expressioni

Gene expression databases

BgeeiENSMUSG00000020307
CleanExiMM_CDC34
ExpressionAtlasiQ8CFI2 baseline and differential
GenevisibleiQ8CFI2 MM

Interactioni

Subunit structurei

Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Identified in a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and RBX1. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened (By similarity). When phosphorylated, interacts with beta-TrCP (BTRC) (By similarity). Interacts with casein kinase subunit CSNK2B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229706, 4 interactors
IntActiQ8CFI2, 3 interactors
STRINGi10090.ENSMUSP00000020550

Structurei

3D structure databases

ProteinModelPortaliQ8CFI2
SMRiQ8CFI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 235SCF-bindingBy similarityAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi200 – 235Asp/Glu-rich (acidic)Add BLAST36

Domaini

The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.By similarity

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0425 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00730000110436
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiQ8CFI2
KOiK02207
OMAiCVKTKTP
OrthoDBiEOG091G0O9F
PhylomeDBiQ8CFI2
TreeFamiTF101107

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CFI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN
60 70 80 90 100
TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP
110 120 130 140 150
VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK
160 170 180 190 200
WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD
210 220 230
EGSDLFYDDY YEDGEVEEAD SCFGDEEDDS GTEES
Length:235
Mass (Da):26,622
Last modified:March 1, 2003 - v1
Checksum:i3259FA0CD407E1E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039160 mRNA Translation: AAH39160.1
BC094502 mRNA Translation: AAH94502.1
CCDSiCCDS23983.1
RefSeqiNP_808281.1, NM_177613.2
XP_017169376.1, XM_017313887.1
XP_017169377.1, XM_017313888.1
UniGeneiMm.21981

Genome annotation databases

EnsembliENSMUST00000020550; ENSMUSP00000020550; ENSMUSG00000020307
ENSMUST00000166603; ENSMUSP00000128806; ENSMUSG00000020307
GeneIDi216150
KEGGimmu:216150
UCSCiuc007fzi.1 mouse

Similar proteinsi

Entry informationi

Entry nameiUB2R1_MOUSE
AccessioniPrimary (citable) accession number: Q8CFI2
Secondary accession number(s): Q505K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health