Ubiquitin-conjugating enzyme E2 R1 - Mus musculus (Mouse)

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Q8CFI2 (UB2R1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 R1
EC=6.3.2.19

Alternative name(s):
Ubiquitin-conjugating enzyme E2-32 kDa complementing
Ubiquitin-conjugating enzyme E2-CDC34
Ubiquitin-protein ligase R1

Gene names

Name:	Cdc34
Synonyms:	Ubch3, Ube2r1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin By similarity. Ref.2
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Enzyme regulation	CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Identified in a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and RBX1. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened By similarity. When phosphorylated, interacts with beta-TrCP (BTRC) By similarity. Interacts with casein kinase subunit CSNK2B By similarity. Ref.2
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase By similarity.
Domain	The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit By similarity.
Post-translational modification	Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein monoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 235

235

Ubiquitin-conjugating enzyme E2 R1

PRO_0000082452

Regions

Region

190 – 235

SCF-binding By similarity

Compositional bias

200 – 235

Asp/Glu-rich (acidic)

Sites

Active site

Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue

203

Phosphoserine; by CK2 By similarity

Modified residue

221

Phosphoserine; by CK2 By similarity

Modified residue

230

Phosphoserine; by CK2 By similarity

Modified residue

232

Phosphothreonine; by CK2 By similarity

Modified residue

235

Phosphoserine; by CK2 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CFI2 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 3259FA0CD407E1E3
Show »

FASTA

235

26,622

        10         20         30         40         50         60 
MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA 

        70         80         90        100        110        120 
RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV 

       130        140        150        160        170        180 
RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG 

       190        200        210        220        230 
VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEAD SCFGDEEDDS GTEES

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: 129 and C57BL/6J. Tissue: Mammary tumor.
[2]	"Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha." Tan P., Fuchs S.Y., Chen A., Wu K., Gomez C., Ronai Z., Pan Z.-Q. Mol. Cell 3:527-533(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SCF COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC039160 mRNA. Translation: AAH39160.1. BC094502 mRNA. Translation: AAH94502.1.
IPI	IPI00229310.
RefSeq	NP_808281.1. NM_177613.2.
UniGene	Mm.21981.
3D structure databases
ProteinModelPortal	Q8CFI2.
SMR	Q8CFI2. Positions 7-183.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CFI2. 3 interactions.
PTM databases
PhosphoSite	Q8CFI2.
Proteomic databases
PaxDb	Q8CFI2.
PRIDE	Q8CFI2.
Protocols and materials databases
DNASU	216150.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020550; ENSMUSP00000020550; ENSMUSG00000020307. ENSMUST00000166603; ENSMUSP00000128806; ENSMUSG00000020307.
GeneID	216150.
KEGG	mmu:216150.
Organism-specific databases
CTD	997.
MGI	MGI:102657. Cdc34.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00530000063512.
HOGENOM	HOG000233454.
HOVERGEN	HBG063308.
InParanoid	Q8CFI2.
KO	K02207.
OMA	EYCIKSK.
OrthoDB	EOG4DBTFM.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q8CFI2.
CleanEx	MM_CDC34.
Genevestigator	Q8CFI2.
GermOnline	ENSMUSG00000020307. Mus musculus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CDC34. mouse.
NextBio	375008.
SOURCE	Search...

Entry information

Entry name

UB2R1_MOUSE

Accession

Primary (citable) accession number: Q8CFI2
Secondary accession number(s): Q505K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents