Reviewed, UniProtKB/Swiss-Prot Q8CFI2 (UB2R1_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 R1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase R1
    Ubiquitin-conjugating enzyme E2-32 kDa complementing
    E2-CDC34

Gene names

Name:	Cdc34
Synonyms:	Ube2r1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC) By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ppp1ca	P62137	1	EBI-1202331,EBI-357187
Ppp1cc	P63087-1	1	EBI-1202331,EBI-450267

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Ubiquitin-conjugating enzyme E2 R1		PRO_0000082452
Regions
	Compositional bias	200 – 235	36	Asp/Glu-rich (acidic)
Sites
	Active site	93	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	230	1	Phosphoserine; by CK2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CFI2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3259FA0CD407E1E3	FASTA	235	26,622
	10 20 30 40 50 60 MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA 70 80 90 100 110 120 RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV 130 140 150 160 170 180 RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG 190 200 210 220 230 VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEAD SCFGDEEDDS GTEES

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: 129 and C57BL/6J. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC039160 mRNA. Translation: AAH39160.1. BC094502 mRNA. Translation: AAH94502.1.
IPI	IPI00229310.
RefSeq	NP_808281.1. XP_001478177.1.
UniGene	Mm.21981
3D structure databases
HSSP	HSSP built from PDB template 1PZV based on UniProtKB P34477.
SMR	Q8CFI2. Positions 9-181.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CFI2. 2 interactions.
PTM databases
PhosphoSite	Q8CFI2.
Proteomic databases
PRIDE	Q8CFI2.
Genome annotation databases
Ensembl	ENSMUSG00000020307. Mus musculus. [Contig view]
GeneID	100046898. 216150.
KEGG	mmu:100046898. mmu:216150.
Organism-specific databases
MGI	MGI:102657. Cdc34.
Phylogenomic databases
HOGENOM	Q8CFI2.
HOVERGEN	Q8CFI2.
OMA	Q8CFI2. HTHVASS.
Enzyme and pathway databases
BRENDA	6.3.2.19. 244.
Gene expression databases
ArrayExpress	Q8CFI2.
Bgee	Q8CFI2.
CleanEx	MM_CDC34.
GermOnline	ENSMUSG00000020307. Mus musculus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	460338.
SOURCE	Search...

Entry information

Entry name

UB2R1_MOUSE

Accession

Primary (citable) accession number: Q8CFI2
Secondary accession number(s): Q505K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)