Q8CFI0 (NED4L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase NEDD4-like
EC=6.3.2.-

Alternative name(s):
NEDD4.2
Nedd4-2

Gene names

Name:	Nedd4l
Synonyms:	Kiaa0439, Nedd4b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1004 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ref.1 Ref.2 Ref.5 Ref.6 Ref.8
Enzyme regulation	Activated by NDFIP1- and NDFIP2-binding By similarity. Interacts with SGK1 By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. Interacts with CLCN5. The phosphorylated form interacts with 14-3-3 proteins. Interacts with NDIF1P in vitro By similarity. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A and SCN10A. Interacts with UBE2E3. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase. Interacts with TNK2 By similarity. Interacts (via C2 domain) with NPC2 By similarity. Ref.2 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm Ref.7.
Tissue specificity	Highly expressed in liver and kidney. Also expressed in heart, brain and lung. Isoform 1 is expressed in kidney, lung and gut. Isoform 3 is ubiquitously expressed. Ref.1 Ref.6
Post-translational modification	Phosphorylated; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation By similarity. Aldosterone induces Ser-477 phosphorylation by SGK1. Ref.5 Ref.10 Auto-ubiquitinated By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 4 WW domains.
Sequence caution	The sequence BAC31307.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Ligase
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of sodium ion transport Inferred from direct assay Ref.1PubMed 17715136. Source: MGI negative regulation of systemic arterial blood pressure Inferred from mutant phenotype PubMed 18524855. Source: MGI positive regulation of cation channel activity Inferred from genetic interaction PubMed 18981174. Source: MGI positive regulation of sodium ion transport Inferred from genetic interaction PubMed 18981174. Source: MGI protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of tight junction assembly Inferred from direct assay PubMed 20504882. Source: MGI response to salt stress Inferred from mutant phenotype PubMed 18524855. Source: MGI ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Inferred from direct assay PubMed 22019085. Source: MGI
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	sodium channel inhibitor activity Inferred from direct assay Ref.1. Source: MGI ubiquitin-protein ligase activity Inferred from direct assay PubMed 20504882. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CFI0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CFI0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Isoform 3 (identifier: Q8CFI0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     385-404: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1004

1004

E3 ubiquitin-protein ligase NEDD4-like

PRO_0000120324

Regions

Domain

35 – 137

103

C2

Domain

221 – 254

34

WW 1

Domain

414 – 447

34

WW 2

Domain

526 – 559

34

WW 3

Domain

577 – 610

34

WW 4

Domain

669 – 1003

335

HECT

Compositional bias

321 – 324

4

Poly-Pro

Sites

Active site

971

1

Glycyl thioester intermediate

Amino acid modifications

Modified residue

331

1

Phosphothreonine Ref.12

Modified residue

347

1

Phosphothreonine By similarity

Modified residue

371

1

Phosphoserine; by WNK1 and WNK4 Ref.5

Modified residue

396

1

Phosphothreonine; by SGK1 By similarity

Modified residue

475

1

Phosphoserine Ref.11

Modified residue

477

1

Phosphoserine; by SGK1 Ref.5 Ref.10 Ref.12

Modified residue

478

1

Phosphoserine; by WNK1 and WNK4 By similarity

Modified residue

493

1

Phosphoserine By similarity

Modified residue

508

1

Phosphoserine Ref.12

Modified residue

512

1

Phosphoserine Ref.12

Modified residue

516

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 149

149

Missing in isoform 2.

VSP_015450

Alternative sequence

385 – 404

20

Missing in isoform 3.

VSP_015453

Experimental info

Mutagenesis

371

1

S → A: Weakly reduces phosphorylation by SGK1. Ref.5

Mutagenesis

477

1

S → A: Strongly reduces phosphorylation by SGK1. Ref.5

Mutagenesis

971

1

C → S: Abolishes catalytic activity. Ref.1

Sequence conflict

179

1

A → G in AAK00809. Ref.1

Sequence conflict

390

1

R → G in AAK00809. Ref.1

Sequence conflict

403

1

P → S in AAK00809. Ref.1

Sequence conflict

585

1

E → G in AAK00809. Ref.1

Sequence conflict

832

1

N → T in AAK00809. Ref.1

Sequence conflict

847

1

N → D in AAK00809. Ref.1

Sequence conflict

949

1

N → K in BAC31307. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1004

Helix Strand Turn

Details...

Beta strand

227 – 231

5

Beta strand

233 – 235

3

Beta strand

237 – 241

5

Turn

242 – 244

3

Beta strand

247 – 250

4

Beta strand

420 – 424

5

Beta strand

426 – 428

3

Beta strand

430 – 434

5

Turn

435 – 438

4

Beta strand

439 – 443

5

Beta strand

532 – 536

5

Beta strand

542 – 546

5

Turn

547 – 550

4

Beta strand

551 – 555

5

Helix

557 – 559

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 50CBB3436052AA60	FASTA	1,004	115,419
	10 20 30 40 50 60 MSLCEAPVHV GDKELKYFQI PQMLSQLSLL ASHHSRGLEF SGGQGESRIL RVKVVSGIDL 70 80 90 100 110 120 AKKDIFGASD PYVKLSLYVA DENRELALVQ TKTIKKTLNP KWNEEFYFRV NPSNHRLLFE 130 140 150 160 170 180 VFDENRLTRD DFLGQVDVPL SHLPTEDPTM ERPYTFKDFL LRPRSHKSRV KGFLRLKMAY 190 200 210 220 230 240 MPKNGGQDEE NSEQRDDMEH GWEVVDSNDS ASQHQEELPP PPLPPGWEEK VDNLGRTYYV 250 260 270 280 290 300 NHNNRSTQWH RPSLMDVSSE SDNNIRQINQ EAAHRRFRSR RHISEDLEPE ASEGGGEGPE 310 320 330 340 350 360 PWETISEEMN MAGDSLSLAL PPPPASPVSR TSPQELSEEV SRRLQITPDS NGEQFSSLIQ 370 380 390 400 410 420 REPSSRLRSC SVTDTVAEQA HLPPPSTPTR RARSSTVTGG EEPTPSVAYV HTTPGLPSGW 430 440 450 460 470 480 EERKDAKGRT YYVNHNNRTT TWTRPIMQLA EDGASGSATN SNNHLVEPQI RRPRSLSSPT 490 500 510 520 530 540 VTLSAPLEGA KDSPIRRAVK DTLSNPQSPQ PSPYNSPKPQ HKVTQSFLPP GWEMRIAPNG 550 560 570 580 590 600 RPFFIDHNTK TTTWEDPRLK FPVHMRSKAS LNPNDLGPLP PGWEERIHLD GRTFYIDHNS 610 620 630 640 650 660 KITQWEDPRL QNPAITGPAV PYSREFKQKY DYFRKKLKKP ADIPNRFEMK LHRNNIFEES 670 680 690 700 710 720 YRRIMSVKRP DVLKARLWIE FESEKGLDYG GVAREWFFLL SKEMFNPYYG LFEYSATDNY 730 740 750 760 770 780 TLQINPNSGL CNEDHLSYFT FIGRVAGLAV FHGKLLDGFF IRPFYKMMLG KQITLNDMES 790 800 810 820 830 840 VDSEYYNSLK WILENDPTEL DLMFCIDEEN FGQTYQVDLK PNGSEIMVTN ENKREYIDLV 850 860 870 880 890 900 IQWRFVNRVQ KQMNAFLEGF TELLPIDLIK IFDENELELL MCGLGDVDVN DWRQHSIYKN 910 920 930 940 950 960 GYCPNHPVIQ WFWKAVLLMD AEKRIRLLQF VTGTSRVPMN GFAELYGSNG PQLFTIEQWG 970 980 990 1000 SPEKLPRAHT CFNRLDLPPY ETFEDLREKL LMAVENAQGF EGVD
	Isoform 2 [UniParc]. Checksum: 96C452B442855895	FASTA	855	98,466
	10 20 30 40 50 60 MERPYTFKDF LLRPRSHKSR VKGFLRLKMA YMPKNGGQDE ENSEQRDDME HGWEVVDSND 70 80 90 100 110 120 SASQHQEELP PPPLPPGWEE KVDNLGRTYY VNHNNRSTQW HRPSLMDVSS ESDNNIRQIN 130 140 150 160 170 180 QEAAHRRFRS RRHISEDLEP EASEGGGEGP EPWETISEEM NMAGDSLSLA LPPPPASPVS 190 200 210 220 230 240 RTSPQELSEE VSRRLQITPD SNGEQFSSLI QREPSSRLRS CSVTDTVAEQ AHLPPPSTPT 250 260 270 280 290 300 RRARSSTVTG GEEPTPSVAY VHTTPGLPSG WEERKDAKGR TYYVNHNNRT TTWTRPIMQL 310 320 330 340 350 360 AEDGASGSAT NSNNHLVEPQ IRRPRSLSSP TVTLSAPLEG AKDSPIRRAV KDTLSNPQSP 370 380 390 400 410 420 QPSPYNSPKP QHKVTQSFLP PGWEMRIAPN GRPFFIDHNT KTTTWEDPRL KFPVHMRSKA 430 440 450 460 470 480 SLNPNDLGPL PPGWEERIHL DGRTFYIDHN SKITQWEDPR LQNPAITGPA VPYSREFKQK 490 500 510 520 530 540 YDYFRKKLKK PADIPNRFEM KLHRNNIFEE SYRRIMSVKR PDVLKARLWI EFESEKGLDY 550 560 570 580 590 600 GGVAREWFFL LSKEMFNPYY GLFEYSATDN YTLQINPNSG LCNEDHLSYF TFIGRVAGLA 610 620 630 640 650 660 VFHGKLLDGF FIRPFYKMML GKQITLNDME SVDSEYYNSL KWILENDPTE LDLMFCIDEE 670 680 690 700 710 720 NFGQTYQVDL KPNGSEIMVT NENKREYIDL VIQWRFVNRV QKQMNAFLEG FTELLPIDLI 730 740 750 760 770 780 KIFDENELEL LMCGLGDVDV NDWRQHSIYK NGYCPNHPVI QWFWKAVLLM DAEKRIRLLQ 790 800 810 820 830 840 FVTGTSRVPM NGFAELYGSN GPQLFTIEQW GSPEKLPRAH TCFNRLDLPP YETFEDLREK 850 LLMAVENAQG FEGVD
	Isoform 3 [UniParc]. Checksum: B6888DF5810B6362	FASTA	984	113,350
	10 20 30 40 50 60 MSLCEAPVHV GDKELKYFQI PQMLSQLSLL ASHHSRGLEF SGGQGESRIL RVKVVSGIDL 70 80 90 100 110 120 AKKDIFGASD PYVKLSLYVA DENRELALVQ TKTIKKTLNP KWNEEFYFRV NPSNHRLLFE 130 140 150 160 170 180 VFDENRLTRD DFLGQVDVPL SHLPTEDPTM ERPYTFKDFL LRPRSHKSRV KGFLRLKMAY 190 200 210 220 230 240 MPKNGGQDEE NSEQRDDMEH GWEVVDSNDS ASQHQEELPP PPLPPGWEEK VDNLGRTYYV 250 260 270 280 290 300 NHNNRSTQWH RPSLMDVSSE SDNNIRQINQ EAAHRRFRSR RHISEDLEPE ASEGGGEGPE 310 320 330 340 350 360 PWETISEEMN MAGDSLSLAL PPPPASPVSR TSPQELSEEV SRRLQITPDS NGEQFSSLIQ 370 380 390 400 410 420 REPSSRLRSC SVTDTVAEQA HLPPPSVAYV HTTPGLPSGW EERKDAKGRT YYVNHNNRTT 430 440 450 460 470 480 TWTRPIMQLA EDGASGSATN SNNHLVEPQI RRPRSLSSPT VTLSAPLEGA KDSPIRRAVK 490 500 510 520 530 540 DTLSNPQSPQ PSPYNSPKPQ HKVTQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK 550 560 570 580 590 600 FPVHMRSKAS LNPNDLGPLP PGWEERIHLD GRTFYIDHNS KITQWEDPRL QNPAITGPAV 610 620 630 640 650 660 PYSREFKQKY DYFRKKLKKP ADIPNRFEMK LHRNNIFEES YRRIMSVKRP DVLKARLWIE 670 680 690 700 710 720 FESEKGLDYG GVAREWFFLL SKEMFNPYYG LFEYSATDNY TLQINPNSGL CNEDHLSYFT 730 740 750 760 770 780 FIGRVAGLAV FHGKLLDGFF IRPFYKMMLG KQITLNDMES VDSEYYNSLK WILENDPTEL 790 800 810 820 830 840 DLMFCIDEEN FGQTYQVDLK PNGSEIMVTN ENKREYIDLV IQWRFVNRVQ KQMNAFLEGF 850 860 870 880 890 900 TELLPIDLIK IFDENELELL MCGLGDVDVN DWRQHSIYKN GYCPNHPVIQ WFWKAVLLMD 910 920 930 940 950 960 AEKRIRLLQF VTGTSRVPMN GFAELYGSNG PQLFTIEQWG SPEKLPRAHT CFNRLDLPPY 970 980 ETFEDLREKL LMAVENAQGF EGVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel mouse Nedd4 protein suppresses the activity of the epithelial Na+ channel." Kamynina E., Debonneville C., Bens M., Vandewalle A., Staub O. FASEB J. 15:204-214(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF CYS-971. Strain: C57BL/6.
[2]	"The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels." Fotia A.B., Dinudom A., Shearwin K.E., Koch J.-P., Korbmacher C., Cook D.I., Kumar S. FASEB J. 17:70-72(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), INTERACTION WITH SCNN1A; SCNN1B AND SCNN1G, FUNCTION. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Kidney and Mammary gland.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 44-1004 (ISOFORM 3). Strain: C57BL/6J. Tissue: Cerebellum.
[5]	"Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression." Debonneville C., Flores S.Y., Kamynina E., Plant P.J., Tauxe C., Thomas M.A., Muenster C., Chraiebi A., Pratt J.H., Horisberger J.-D., Pearce D., Loffing J., Staub O. EMBO J. 20:7052-7059(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SCNN1A; SCNN1B AND SCNN1G, PHOSPHORYLATION AT SER-371 AND SER-477, MUTAGENESIS OF SER-371 AND SER-477.
[6]	"The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel." Harvey K.F., Dinudom A., Cook D.I., Kumar S. J. Biol. Chem. 276:8597-8601(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SCNN1A; SCNN1B AND SCNN1G, TISSUE SPECIFICITY.
[7]	"Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein." Konstas A.-A., Shearwin-Whyatt L.M., Fotia A.B., Degger B., Riccardi D., Cook D.I., Korbmacher C., Kumar S. J. Biol. Chem. 277:29406-29416(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NDFIP2, SUBCELLULAR LOCATION.
[8]	"Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2." Fotia A.B., Ekberg J., Adams D.J., Cook D.I., Poronnik P., Kumar S. J. Biol. Chem. 279:28930-28935(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SCN1A; SCN2A; SCN3A; SCN5A; SCN8A; SCN9A; SCN10A; SCNN1A; SCNN1B AND SCNN1G.
[9]	"Participation of the ubiquitin-conjugating enzyme UBE2E3 in Nedd4-2-dependent regulation of the epithelial Na+ channel." Debonneville C., Staub O. Mol. Cell. Biol. 24:2397-2409(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBE2E3.
[10]	"Aldosterone-induced serum and glucocorticoid-induced kinase 1 expression is accompanied by nedd4-2 phosphorylation and increased na+ transport in cortical collecting duct cells." Flores S.Y., Loffing-Cueni D., Kamynina E., Daidie D., Gerbex C., Chabanel S., Dudler J., Loffing J., Staub O. J. Am. Soc. Nephrol. 16:2279-2287(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-477.
[11]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-475, MASS SPECTROMETRY. Tissue: Brain cortex.
[12]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-331; SER-477; SER-508 AND SER-512, MASS SPECTROMETRY. Tissue: Liver.
[13]	"Solution structures of WW domains of NEDD4-2." Kowalski K., Merkel A.L., Booker G.W. Submitted (OCT-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 221-254; 414-447 AND 525-560.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF277232 mRNA. Translation: AAK00809.1.
BC039746 mRNA. Translation: AAH39746.1.
BC071210 mRNA. Translation: AAH71210.1.
AK042621 mRNA. Translation: BAC31307.1. Different initiation.

IPI

IPI00404545.
IPI00649115.
IPI01008452.

UniGene

Mm.98668.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WR3	NMR	-	A	221-254	[»]
1WR4	NMR	-	A	414-447	[»]
1WR7	NMR	-	A	525-560	[»]

ProteinModelPortal

Q8CFI0.

SMR

Q8CFI0. Positions 40-182, 221-254, 415-449, 525-995.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48843N.

Protein family/group databases

TCDB

8.A.30.1.1. Nedd4-family interacting protein-2 (Nedd4) family.

PTM databases

PhosphoSite

Q8CFI0.

Proteomic databases

PaxDb

Q8CFI0.

PRIDE

Q8CFI0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

UCSC

uc008fen.2. mouse.
uc008fer.2. mouse.
uc012beb.1. mouse.

Organism-specific databases

MGI

MGI:1933754. Nedd4l.

Rouge

Search...

Phylogenomic databases

eggNOG

COG5021.

HOGENOM

HOG000208451.

HOVERGEN

HBG004134.

InParanoid

Q8CFI0.

OrthoDB

EOG4QRH3C.

Enzyme and pathway databases

UniPathway

UPA00143.

Gene expression databases

Genevestigator

Q8CFI0.

GermOnline

ENSMUSG00000024589. Mus musculus.

Family and domain databases

InterPro

IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR020477. C2_dom.
IPR018029. C2_membr_targeting.
IPR024928. E3_ub_ligase_SMURF1.
IPR000569. HECT.
IPR001202. WW_dom.
[Graphical view]

Pfam

PF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF00397. WW. 4 hits.
[Graphical view]

PIRSF

PIRSF001569. E3_ub_ligase_SMURF1. 1 hit.

PRINTS

PR00360. C2DOMAIN.

SMART

SM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 4 hits.
[Graphical view]

SUPFAM

SSF49562. C2_CaLB. 1 hit.
SSF56204. HECT. 1 hit.
SSF51045. WW_Rsp5_WWP. 4 hits.

PROSITE

PS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 4 hits.
PS50020. WW_DOMAIN_2. 4 hits.
[Graphical view]

ProtoNet

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Other

ChiTaRS

NEDD4L. mouse.

EvolutionaryTrace

Q8CFI0.

SOURCE

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Entry information

Entry name

NED4L_MOUSE

Accession

Primary (citable) accession number: Q8CFI0
Secondary accession number(s): Q8BRT9, Q8BS42, Q99PK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program