Serine/threonine-protein kinase SIK2

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Q8CFH6 (SIK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SIK2
EC=2.7.11.1

Alternative name(s):
Salt-inducible kinase 2
Short name=SIK-2
Serine/threonine-protein kinase SNF1-like kinase 2

Gene names

Name:	Sik2
Synonyms:	Snf1lk2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	931 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates 'Ser-789' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators. Ref.1 Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1
Cofactor	Magnesium. Ref.1
Enzyme regulation	Activated by phosphorylation on Thr-175 By similarity. Ref.2
Subunit structure	Interacts with and phosphorylates TORC2/CRTC2 By similarity.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Present in both white and brown adipose tissues with levels increasing during adipocyte differentiation. Lower levels observed in the testis. Ref.1
Post-translational modification	Phosphorylated at Thr-175 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 By similarity. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. Contains 1 UBA domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway Inferred from direct assay Ref.1. Source: MGI intracellular protein kinase cascade Inferred from direct assay Ref.1. Source: UniProtKB protein autophosphorylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of insulin receptor signaling pathway Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	ATP binding Inferred from direct assay Ref.1. Source: UniProtKB magnesium ion binding Inferred from direct assay Ref.1. Source: UniProtKB protein serine/threonine kinase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 931

931

Serine/threonine-protein kinase SIK2

PRO_0000086663

Regions

Domain

20 – 271

252

Protein kinase

Domain

295 – 335

UBA

Nucleotide binding

26 – 34

ATP By similarity UniProtKB Q13131

Sites

Active site

142

Proton acceptor By similarity UniProtKB Q13131

Binding site

ATP Ref.1

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

175

Phosphothreonine By similarity UniProtKB Q9H0K1

Modified residue

587

Phosphoserine By similarity

Experimental info

Mutagenesis

K → M: Loss of kinase activity. Ref.1 Ref.2

Mutagenesis

175

T → E: Low levels of constitutive activity. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CFH6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5CF2FB8DCDC689F4
Show »

FASTA

931

104,198

        10         20         30         40         50         60 
MVMADGPRHL QRGPVRVGFY DIEGTLGKGN FAVVKLGRHR TTKTEVAIKI IDKSQLDAVN 

        70         80         90        100        110        120 
LEKIYREVQI MKMLDHPHII KLYQVMETKS MLYLVTEYAK NGEIFDYLAN HGRLNESEAR 

       130        140        150        160        170        180 
RKFWQILSAV DYCHGRKVVH RDLKAENLLL DNNMNIKIAD FGFGNFFKTG ELLATWCGSP 

       190        200        210        220        230        240 
PYAAPEVFEG QQYEGPQLDI WSMGVVLYVL VCGALPFDGP TLPILRQRVL EGRFRIPYFM 

       250        260        270        280        290        300 
SEDCEHLIRR MLVLDPSKRL SIAQIKEHKW MLIEVPVQRP ILYPQEQENE PSIGEFNEQV 

       310        320        330        340        350        360 
LRLMHSLGID QQKTVESLQN KSYNHFAAIY FLLVERLKSH RSSFPVEQRL DGRQRRPSTI 

       370        380        390        400        410        420 
AEQTVAKAQT VGLPVTLHPP NVRLMRSTLL PQASNVEAFS FPTSSCQAEA AFMEEECVDT 

       430        440        450        460        470        480 
PKVNGCLLDP VPPVLVRKGC QSLPSSMMET SIDEGLETEG EAEEDPSQAF EAFQATRSGQ 

       490        500        510        520        530        540 
RRHTLSEVTN QLVVMPGAGK MFSMSDNPSL ESVDSEYDMG SAQRDLNFLE DSPSLKDIML 

       550        560        570        580        590        600 
ANQPSPRMTS PFISLRPANP AMQALSSQKR EAHNRSPVSF REGRRASDTS LTQGIVAFRQ 

       610        620        630        640        650        660 
HLQNLARTKG ILELNKVQLL YEQMGSNADP TLTSTAPQLQ DLSSSCPQEE ISQQQESVSS 

       670        680        690        700        710        720 
LSASMHPQLS PQQSLETQYL QHRLQKPNLL PKAQSPCPVY CKEPPRSLEQ QLQEHRLQQK 

       730        740        750        760        770        780 
RLFLQKQSQL QAYFNQMQIA ESSYPGPSQQ LALPHQETPL TSQQPPSFSL TQALSPVLEP 

       790        800        810        820        830        840 
SSEQMQFSSF LSQYPEMQLQ PLPSTPGPRA PPPLPSQLQQ HQQPPPPPPP PPPQQPGAAP 

       850        860        870        880        890        900 
TSLQFSYQTC ELPSTTSSVP NYPASCHYPV DGAQQSNLTG ADCPRSSGLQ DTASSYDPLA 

       910        920        930 
LSELPGLFDC EMVEAVDPQH NGVVSCLARE T

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References

[1]	"Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2." Horike N., Takemori H., Katoh Y., Doi J., Min L., Asano T., Sun X.J., Yamamoto H., Kasayama S., Muraoka M., Nonaka Y., Okamoto M. J. Biol. Chem. 278:18440-18447(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-49. Tissue: Adipose tissue.
[2]	"Silencing the constitutive active transcription factor CREB by the LKB1-SIK signaling cascade." Katoh Y., Takemori H., Lin X.-Z., Tamura M., Muraoka M., Satoh T., Tsuchiya Y., Min L., Doi J., Miyauchi A., Witters L.A., Nakamura H., Okamoto M. FEBS J. 273:2730-2748(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, PHOSPHORYLATION AT THR-175 AND SER-587, MUTAGENESIS OF LYS-49 AND THR-175.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB067780 mRNA. Translation: BAC53845.1.
IPI	IPI00229305.
RefSeq	NP_848825.2. NM_178710.3.
UniGene	Mm.104932.
3D structure databases
ProteinModelPortal	Q8CFH6.
SMR	Q8CFH6. Positions 17-333.
ModBase	Search...
PTM databases
PhosphoSite	Q8CFH6.
Proteomic databases
PaxDb	Q8CFH6.
PRIDE	Q8CFH6.
Protocols and materials databases
DNASU	235344.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	235344.
KEGG	mmu:235344.
UCSC	uc009pky.1. mouse.
Organism-specific databases
CTD	23235.
MGI	MGI:2445031. Sik2.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000048716.
InParanoid	Q8CFH6.
KO	K16311.
OrthoDB	EOG42JNQV.
Gene expression databases
CleanEx	MM_SNF1LK2.
Genevestigator	Q8CFH6.
GermOnline	ENSMUSG00000037112. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. IPR017090. Ser/Thr_kinase_SIK1/2. IPR015940. UBA/transl_elong_EF1B_N_euk. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037014. Ser/Thr_PK_SNF1-like. 1 hit.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50030. UBA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	382621.
SOURCE	Search...

Entry information

Entry name

SIK2_MOUSE

Accession

Primary (citable) accession number: Q8CFH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents