Reviewed, UniProtKB/Swiss-Prot Q8CFG9 (C1RB_MOUSE)

Names and origin

Protein names

Recommended name:
    Complement C1r-B subcomponent
    EC=3.4.21.41
Alternative name(s):
    Complement component 1, r-B subcomponent
Cleaved into the following 2 chains:
    1- Recommended name:
            Complement C1r-B subcomponent heavy chain
    2- Recommended name:
            Complement C1r-B subcomponent light chain

Gene names

Name:	C1rb
Synonyms:	C1r

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system By similarity.
Catalytic activity	Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).
Subunit structure	C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds By similarity.
Tissue specificity	Specifically expressed in male reproductive tissues. Ref.1
Post-translational modification	The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Biological process	Complement pathway Immune response Innate immunity
Domain	EGF-like domain Repeat Signal Sushi
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Hydroxylation Phosphoprotein
Gene Ontology (GO)
Biological process	complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	By similarity
	Chain	17 – 706	690	Complement C1r-B subcomponent		PRO_0000042187
	Chain	17 – 462	446	Complement C1r-B subcomponent heavy chain By similarity		PRO_0000042188
	Chain	463 – 706	244	Complement C1r-B subcomponent light chain By similarity		PRO_0000042189
Regions
	Domain	17 – 140	124	CUB 1
	Domain	141 – 189	49	EGF-like; calcium-binding Potential
	Domain	192 – 304	113	CUB 2
	Domain	306 – 372	67	Sushi 1
	Domain	373 – 448	76	Sushi 2
	Domain	463 – 703	241	Peptidase S1
Sites
	Active site	501	1	Charge relay system By similarity
	Active site	558	1	Charge relay system By similarity
	Active site	655	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	166	1	(3R)-3-hydroxyasparagine By similarity
	Modified residue	205	1	Phosphoserine; by CK2 By similarity
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	582	1	N-linked (GlcNAc...) Potential
	Disulfide bond	70 ↔ 88		By similarity
	Disulfide bond	145 ↔ 164		By similarity
	Disulfide bond	160 ↔ 173		By similarity
	Disulfide bond	175 ↔ 188		By similarity
	Disulfide bond	192 ↔ 219		By similarity
	Disulfide bond	249 ↔ 267		By similarity
	Disulfide bond	308 ↔ 357		By similarity
	Disulfide bond	337 ↔ 370		By similarity
	Disulfide bond	375 ↔ 428		By similarity
	Disulfide bond	405 ↔ 446		By similarity
	Disulfide bond	450 ↔ 578		Interchain (between heavy and light chains) By similarity
	Disulfide bond	621 ↔ 640		By similarity
	Disulfide bond	651 ↔ 681		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CFG9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 092684922F41A64F	FASTA	706	79,936
	10 20 30 40 50 60 MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP MGYRVKLVFW 70 80 90 100 110 120 QFDVEPSEGC LYDYVKISAD KQTLGRFCGQ LDSPLGNPPG SKEFMSQGNK MLLTFHTDFS 130 140 150 160 170 180 NEENGTIMFY KGFLAYYQAV DLDECASQPN SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE 190 200 210 220 230 240 LQKDGQSCQA ECSSELYTEP SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE 250 260 270 280 290 300 IDDHQQVHCP YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH 310 320 330 340 350 360 YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFTVTCKQG YQLMEGNQAL LSFTAVCQHD 370 380 390 400 410 420 GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS IQYHCHEPYY KMLTRAGSSE 430 440 450 460 470 480 SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC GKPVNPVTQK ERIIGGQPAR PGNFPWQAFT 490 500 510 520 530 540 TIYGPGGGAL LGDRWILTAA HTIYPKYPNK GKNTNPRTLV FLGHTNMEQI QKLGHHPVRR 550 560 570 580 590 600 VIIHPDYRQE EPDNFEGDIA LLELENSVTL GPELLPICLP DNETFYGQGL MGYVSGFGTT 610 620 630 640 650 660 GNRIPFHLRF VRLPVADREA CQRWLWTKKD TSPFSQNMFC SGDPAVQQDA CQGDSGGVFA 670 680 690 700 VRDRNRDIWV ATGIVSWGIG CGEGYGFYTK VLNYVDWIKK EMGDEN

References

[1]

"Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system." Garnier G., Circolo A., Xu Y., Volanakis J.E. Biochem. J. 371:631-640(2003) [PubMed: 12513694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF459018 mRNA. Translation: AAO15557.1.
IPI	IPI00753391.
RefSeq	NP_001106827.1.
UniGene	Mm.476090
3D structure databases
HSSP	HSSP built from PDB template 1GPZ based on UniProtKB P00736.
SMR	Q8CFG9. Positions 306-702.
ModBase	Search...
Protein family/group databases
MEROPS	S01.209.
Genome annotation databases
Ensembl	ENSMUSG00000055172. Mus musculus. [Contig view]
GeneID	667277.
KEGG	mmu:667277.
Phylogenomic databases
HOVERGEN	Q8CFG9.
Enzyme and pathway databases
BRENDA	3.4.21.41. 244.
Gene expression databases
Bgee	Q8CFG9.
CleanEx	MM_C1R.
GermOnline	ENSMUSG00000055172. Mus musculus.
Family and domain databases
InterPro	IPR016060. Complement_control_module. IPR000859. CUB. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_CS. IPR001881. EGF_Ca_bd. IPR013091. EGF_Ca_bd_2. IPR018097. EGF_Ca_bd_CS. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 1 hit. G3DSA:2.60.120.290. CUB. 2 hits.
Pfam	PF00431. CUB. 2 hits. PF07645. EGF_CA. 1 hit. PF00084. Sushi. 2 hits. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00032. CCP. 2 hits. SM00042. CUB. 2 hits. SM00179. EGF_CA. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS01180. CUB. 2 hits. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. PS50923. SUSHI. 2 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	432003.

Entry information

Entry name

C1RB_MOUSE

Accession

Primary (citable) accession number: Q8CFG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)