Reviewed,
UniProtKB/Swiss-Prot Q8CFG9 (C1RB_MOUSE)
Last modified
June 16, 2009.
Version 54.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Complement C1r-B subcomponent EC=3.4.21.41 Alternative name(s): Complement component 1, r-B subcomponent Cleaved into the following 2 chains: 1- Recommended name: Complement C1r-B subcomponent heavy chain 2- Recommended name: Complement C1r-B subcomponent light chain | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 706 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system By similarity. |
| Catalytic activity | Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42). |
| Subunit structure | C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds By similarity. |
| Tissue specificity | Specifically expressed in male reproductive tissues. Ref.1 |
| Post-translational modification | The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity. |
| Sequence similarities | Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Complement pathway Immune response Innate immunity |
| Domain | EGF-like domain Repeat Signal Sushi |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein Hydroxylation Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW innate immune responseInferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 16 | 16 | By similarity | ||||||||
| Chain | 17 – 706 | 690 | Complement C1r-B subcomponent | PRO_0000042187 | |||||||
| Chain | 17 – 462 | 446 | Complement C1r-B subcomponent heavy chain By similarity | PRO_0000042188 | |||||||
| Chain | 463 – 706 | 244 | Complement C1r-B subcomponent light chain By similarity | PRO_0000042189 | |||||||
Regions | |||||||||||
| Domain | 17 – 140 | 124 | CUB 1 | ||||||||
| Domain | 141 – 189 | 49 | EGF-like; calcium-binding Potential | ||||||||
| Domain | 192 – 304 | 113 | CUB 2 | ||||||||
| Domain | 306 – 372 | 67 | Sushi 1 | ||||||||
| Domain | 373 – 448 | 76 | Sushi 2 | ||||||||
| Domain | 463 – 703 | 241 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 501 | 1 | Charge relay system By similarity | ||||||||
| Active site | 558 | 1 | Charge relay system By similarity | ||||||||
| Active site | 655 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 166 | 1 | (3R)-3-hydroxyasparagine By similarity | ||||||||
| Modified residue | 205 | 1 | Phosphoserine; by CK2 By similarity | ||||||||
| Glycosylation | 124 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 220 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 582 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 70 ↔ 88 | By similarity | |||||||||
| Disulfide bond | 145 ↔ 164 | By similarity | |||||||||
| Disulfide bond | 160 ↔ 173 | By similarity | |||||||||
| Disulfide bond | 175 ↔ 188 | By similarity | |||||||||
| Disulfide bond | 192 ↔ 219 | By similarity | |||||||||
| Disulfide bond | 249 ↔ 267 | By similarity | |||||||||
| Disulfide bond | 308 ↔ 357 | By similarity | |||||||||
| Disulfide bond | 337 ↔ 370 | By similarity | |||||||||
| Disulfide bond | 375 ↔ 428 | By similarity | |||||||||
| Disulfide bond | 405 ↔ 446 | By similarity | |||||||||
| Disulfide bond | 450 ↔ 578 | Interchain (between heavy and light chains) By similarity | |||||||||
| Disulfide bond | 621 ↔ 640 | By similarity | |||||||||
| Disulfide bond | 651 ↔ 681 | By similarity | |||||||||
Sequences
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References
| [1] | "Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system." Garnier G., Circolo A., Xu Y., Volanakis J.E. Biochem. J. 371:631-640(2003) [PubMed: 12513694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6. |
Cross-references
Sequence databases | |
|---|---|
| AF459018 mRNA. Translation: AAO15557.1. | |
| IPI | IPI00753391. |
| RefSeq | NP_001106827.1. |
| UniGene | Mm.476090 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GPZ based on UniProtKB P00736. |
| SMR | Q8CFG9. Positions 306-702. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S01.209. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000055172. Mus musculus. [Contig view] |
| GeneID | 667277. |
| KEGG | mmu:667277. |
Phylogenomic databases | |
| HOVERGEN | Q8CFG9. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.41. 244. |
Gene expression databases | |
| Bgee | Q8CFG9. |
| CleanEx | MM_C1R. |
| GermOnline | ENSMUSG00000055172. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016060. Complement_control_module. IPR000859. CUB. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_CS. IPR001881. EGF_Ca_bd. IPR013091. EGF_Ca_bd_2. IPR018097. EGF_Ca_bd_CS. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR000436. Sushi_SCR_CCP. [Graphical view] |
| Gene3D | G3DSA:2.10.70.10. Complement_control_module. 1 hit. G3DSA:2.60.120.290. CUB. 2 hits. |
| Pfam | PF00431. CUB. 2 hits. PF07645. EGF_CA. 1 hit. PF00084. Sushi. 2 hits. PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00032. CCP. 2 hits. SM00042. CUB. 2 hits. SM00179. EGF_CA. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS00010. ASX_HYDROXYL. 1 hit. PS01180. CUB. 2 hits. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. PS50923. SUSHI. 2 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 432003. |
Entry information
| Entry name | C1RB_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8CFG9 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


