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Q8CFG6

- CA2D2_RAT

UniProt

Q8CFG6 - CA2D2_RAT

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Protein

Voltage-dependent calcium channel subunit alpha-2/delta-2

Gene

Cacna2d2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi300 – 3001Divalent metal cationBy similarity
Metal bindingi302 – 3021Divalent metal cationBy similarity
Metal bindingi304 – 3041Divalent metal cationBy similarity

GO - Molecular functioni

  1. calcium channel regulator activity Source: RGD
  2. metal ion binding Source: UniProtKB-KW
  3. voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  1. calcium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_232206. Adrenaline,noradrenaline inhibits insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-2
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-2
Cleaved into the following 2 chains:
Gene namesi
Name:Cacna2d2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi631360. Cacna2d2.

Subcellular locationi

Membrane Curated; Single-pass type I membrane protein Curated
Note: Colocalizes with CACNA1A in lipid raft fractions.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 11191101ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1120 – 114021HelicalSequence AnalysisAdd
BLAST
Topological domaini1141 – 115717CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 11571139Voltage-dependent calcium channel subunit alpha-2/delta-2PRO_0000304643Add
BLAST
Chaini19 – 1004986Voltage-dependent calcium channel subunit alpha-2-2Sequence AnalysisPRO_0000304644Add
BLAST
Chaini1005 – 1157153Voltage-dependent calcium channel subunit delta-2Sequence AnalysisPRO_0000304645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi446 ↔ 1104Interchain (between alpha-2-2 and delta-2 chains)By similarity
Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi543 – 5431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi627 – 6271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi864 – 8641N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity
May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8CFG6.
PRIDEiQ8CFG6.

Expressioni

Tissue specificityi

In heart, it is highly expressed in atrium and at lower level in ventricle.2 Publications

Gene expression databases

GenevestigatoriQ8CFG6.

Interactioni

Subunit structurei

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8CFG6. 4 interactions.
STRINGi10116.ENSRNOP00000021216.

Structurei

3D structure databases

ProteinModelPortaliQ8CFG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini294 – 472179VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini488 – 57790CacheAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi300 – 3045MIDAS-like motif

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Contains 1 cache domain.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307080.
HOGENOMiHOG000004860.
HOVERGENiHBG057779.
InParanoidiQ8CFG6.
KOiK04859.
PhylomeDBiQ8CFG6.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFG6-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MAVPARTCGA SWPGPVRTAR PWPGRGPRPC PDPRGPASGP ARPLLLLLPP
60 70 80 90 100
LLLLPLLTAP GASAYSFPQQ HTMQHWARRL EQEIDGVMRI FGGVQQLREI
110 120 130 140 150
YKDNRNLFDV QENEPQKLVE KVAGDIESLL DRKVQALKRL ADAAENFQKA
160 170 180 190 200
HRWQDNIKEE DIMYYDAKAD AELDDPESED MERGSKTSAL RLDFIEEPNF
210 220 230 240 250
KNKVNYSYTA VQIPTDIYKG STVILNELNW TEALENVFIE NRRQDPTLLW
260 270 280 290 300
QVFGSATGVT RYYPATPWRA PKKIDLYDVR RRPWYIQGAS SPKDMVIIVD
310 320 330 340 350
VSGSVSGLTL KLMKTSVCEM LDTLSDDDYV NVASFNEKAQ PVSCFTHLVQ
360 370 380 390 400
ANVRNKKVFK EAVQGMVAKG TTGYKAGFEY AFDQLQNSNI TRANCNKMIM
410 420 430 440 450
MFTDGGEDRV QDVFEKYNWP NRTVRVFTFS VGQHNYDVTP LQWMACTNKG
460 470 480 490 500
YYFEIPSIGA IRINTQEYLD VLGRPMVLAG KDAKQVQWTN VYEDALGLGL
510 520 530 540 550
VVTGTLPVFN LTQDGPGDKK NQLILGVMGI DVALNDIKRL TPNYTLGANG
560 570 580 590 600
YVFAIDLNGY VLLHPNLKPQ ITNFREPVTL DFLDAELEDE NKEEIRRSMI
610 620 630 640 650
DGDKGHKQIR TLVKSLDERY IDEVIRNYTW VPIRSTNYSL GLVLPPYSTY
660 670 680 690 700
YLQANLSDQI LQVKLPISKL KDFEFLLPSS FESEGHVFIA PREYCKDLNA
710 720 730 740 750
SDNNTEFLKN FIELMEKVTP DSKQCNNFLL HNLILDTGIT QQLVERVWRD
760 770 780 790 800
QDLNTYSLLA VFAATDGGIT RVFPNKAAED WTENPEPFNA SFYRRSLDNR
810 820 830 840 850
GYIFKPPHQD SLLRPLELEN DTVGVLVSTA VELSLGRRTL RPAVVGVKLD
860 870 880 890 900
LEAWAEKFKV LASNRTHQDQ PQKQCGPSSH CEMDCEVNNE DLLCVLIDDG
910 920 930 940 950
GFLVLSNQNH QWDQVGRFFS EVDANLMLAL YNNSFYTRKE SYDYQAACAP
960 970 980 990 1000
QPPGNLGAAP RGVFVPTIAD FLNLAWWTSA AAWSLFQQLL YGLIYHSWFQ
1010 1020 1030 1040 1050
ADPAEAEGSP ETRESSCVMK QTQYYFGSVN ASYNAIIDCG NCSRLFHAQR
1060 1070 1080 1090 1100
LTNTNLLFVV AEKPLCSQCE VGRLLQKETH CPADGPEQCE LVQRPRYRTG
1110 1120 1130 1140 1150
PHICFDYNAT EDTSDCGRGA SFPPSLGVLV SLQLLLLLGL PPRPQPQIHS

FTPSRRL
Length:1,157
Mass (Da):130,776
Last modified:March 1, 2003 - v1
Checksum:i5E81446A1E1EBAF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486277 mRNA. Translation: AAO14653.1.
RefSeqiNP_783182.1. NM_175592.2.
UniGeneiRn.91563.

Genome annotation databases

GeneIDi300992.
KEGGirno:300992.
UCSCiRGD:631360. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486277 mRNA. Translation: AAO14653.1 .
RefSeqi NP_783182.1. NM_175592.2.
UniGenei Rn.91563.

3D structure databases

ProteinModelPortali Q8CFG6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8CFG6. 4 interactions.
STRINGi 10116.ENSRNOP00000021216.

Proteomic databases

PaxDbi Q8CFG6.
PRIDEi Q8CFG6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 300992.
KEGGi rno:300992.
UCSCi RGD:631360. rat.

Organism-specific databases

CTDi 9254.
RGDi 631360. Cacna2d2.

Phylogenomic databases

eggNOGi NOG307080.
HOGENOMi HOG000004860.
HOVERGENi HBG057779.
InParanoidi Q8CFG6.
KOi K04859.
PhylomeDBi Q8CFG6.

Enzyme and pathway databases

Reactomei REACT_232206. Adrenaline,noradrenaline inhibits insulin secretion.

Miscellaneous databases

NextBioi 647862.
PROi Q8CFG6.

Gene expression databases

Genevestigatori Q8CFG6.

Family and domain databases

Gene3Di 3.40.50.410. 1 hit.
InterProi IPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view ]
SMARTi SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
PROSITEi PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1."
    Chu P.-J., Best P.M.
    J. Mol. Cell. Cardiol. 35:207-215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Heart atrium.
  2. "Molecular and electrophysiological differences in the L-type Ca2+ channel of the atrium and ventricle of rat hearts."
    Hatano S., Yamashita T., Sekiguchi A., Iwasaki Y., Nakazawa K., Sagara K., Iinuma H., Aizawa T., Fu L.-T.
    Jpn. Circ. J. 70:610-614(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCA2D2_RAT
AccessioniPrimary (citable) accession number: Q8CFG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds gabapentin, an antiepileptic drug.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3