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Protein

Voltage-dependent calcium channel subunit alpha-2/delta-2

Gene

Cacna2d2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi300Divalent metal cationBy similarity1
Metal bindingi302Divalent metal cationBy similarity1
Metal bindingi304Divalent metal cationBy similarity1

GO - Molecular functioni

  • calcium channel regulator activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  • calcium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-2
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-2
Cleaved into the following 2 chains:
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631360. Cacna2d2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 1119ExtracellularSequence analysisAdd BLAST1101
Transmembranei1120 – 1140HelicalSequence analysisAdd BLAST21
Topological domaini1141 – 1157CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

  • voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000030464319 – 1157Voltage-dependent calcium channel subunit alpha-2/delta-2Add BLAST1139
ChainiPRO_000030464419 – 1004Voltage-dependent calcium channel subunit alpha-2-2Sequence analysisAdd BLAST986
ChainiPRO_00003046451005 – 1157Voltage-dependent calcium channel subunit delta-2Sequence analysisAdd BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi205N-linked (GlcNAc...)Sequence analysis1
Glycosylationi389N-linked (GlcNAc...)Sequence analysis1
Glycosylationi421N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi446 ↔ 1104Interchain (between alpha-2-2 and delta-2 chains)By similarity
Glycosylationi510N-linked (GlcNAc...)Sequence analysis1
Glycosylationi543N-linked (GlcNAc...)Sequence analysis1
Glycosylationi627N-linked (GlcNAc...)Sequence analysis1
Glycosylationi864N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity
May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8CFG6.
PRIDEiQ8CFG6.

Expressioni

Tissue specificityi

In heart, it is highly expressed in atrium and at lower level in ventricle.2 Publications

Interactioni

Subunit structurei

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8CFG6. 3 interactors.
STRINGi10116.ENSRNOP00000021216.

Structurei

3D structure databases

ProteinModelPortaliQ8CFG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 472VWFAPROSITE-ProRule annotationAdd BLAST179
Domaini488 – 577CacheAdd BLAST90

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi300 – 304MIDAS-like motif5

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Contains 1 cache domain.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2353. Eukaryota.
ENOG410XPDX. LUCA.
HOGENOMiHOG000004860.
HOVERGENiHBG057779.
InParanoidiQ8CFG6.
KOiK04859.
PhylomeDBiQ8CFG6.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPARTCGA SWPGPVRTAR PWPGRGPRPC PDPRGPASGP ARPLLLLLPP
60 70 80 90 100
LLLLPLLTAP GASAYSFPQQ HTMQHWARRL EQEIDGVMRI FGGVQQLREI
110 120 130 140 150
YKDNRNLFDV QENEPQKLVE KVAGDIESLL DRKVQALKRL ADAAENFQKA
160 170 180 190 200
HRWQDNIKEE DIMYYDAKAD AELDDPESED MERGSKTSAL RLDFIEEPNF
210 220 230 240 250
KNKVNYSYTA VQIPTDIYKG STVILNELNW TEALENVFIE NRRQDPTLLW
260 270 280 290 300
QVFGSATGVT RYYPATPWRA PKKIDLYDVR RRPWYIQGAS SPKDMVIIVD
310 320 330 340 350
VSGSVSGLTL KLMKTSVCEM LDTLSDDDYV NVASFNEKAQ PVSCFTHLVQ
360 370 380 390 400
ANVRNKKVFK EAVQGMVAKG TTGYKAGFEY AFDQLQNSNI TRANCNKMIM
410 420 430 440 450
MFTDGGEDRV QDVFEKYNWP NRTVRVFTFS VGQHNYDVTP LQWMACTNKG
460 470 480 490 500
YYFEIPSIGA IRINTQEYLD VLGRPMVLAG KDAKQVQWTN VYEDALGLGL
510 520 530 540 550
VVTGTLPVFN LTQDGPGDKK NQLILGVMGI DVALNDIKRL TPNYTLGANG
560 570 580 590 600
YVFAIDLNGY VLLHPNLKPQ ITNFREPVTL DFLDAELEDE NKEEIRRSMI
610 620 630 640 650
DGDKGHKQIR TLVKSLDERY IDEVIRNYTW VPIRSTNYSL GLVLPPYSTY
660 670 680 690 700
YLQANLSDQI LQVKLPISKL KDFEFLLPSS FESEGHVFIA PREYCKDLNA
710 720 730 740 750
SDNNTEFLKN FIELMEKVTP DSKQCNNFLL HNLILDTGIT QQLVERVWRD
760 770 780 790 800
QDLNTYSLLA VFAATDGGIT RVFPNKAAED WTENPEPFNA SFYRRSLDNR
810 820 830 840 850
GYIFKPPHQD SLLRPLELEN DTVGVLVSTA VELSLGRRTL RPAVVGVKLD
860 870 880 890 900
LEAWAEKFKV LASNRTHQDQ PQKQCGPSSH CEMDCEVNNE DLLCVLIDDG
910 920 930 940 950
GFLVLSNQNH QWDQVGRFFS EVDANLMLAL YNNSFYTRKE SYDYQAACAP
960 970 980 990 1000
QPPGNLGAAP RGVFVPTIAD FLNLAWWTSA AAWSLFQQLL YGLIYHSWFQ
1010 1020 1030 1040 1050
ADPAEAEGSP ETRESSCVMK QTQYYFGSVN ASYNAIIDCG NCSRLFHAQR
1060 1070 1080 1090 1100
LTNTNLLFVV AEKPLCSQCE VGRLLQKETH CPADGPEQCE LVQRPRYRTG
1110 1120 1130 1140 1150
PHICFDYNAT EDTSDCGRGA SFPPSLGVLV SLQLLLLLGL PPRPQPQIHS

FTPSRRL
Length:1,157
Mass (Da):130,776
Last modified:March 1, 2003 - v1
Checksum:i5E81446A1E1EBAF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486277 mRNA. Translation: AAO14653.1.
RefSeqiNP_783182.1. NM_175592.2.
UniGeneiRn.91563.

Genome annotation databases

GeneIDi300992.
KEGGirno:300992.
UCSCiRGD:631360. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486277 mRNA. Translation: AAO14653.1.
RefSeqiNP_783182.1. NM_175592.2.
UniGeneiRn.91563.

3D structure databases

ProteinModelPortaliQ8CFG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CFG6. 3 interactors.
STRINGi10116.ENSRNOP00000021216.

Proteomic databases

PaxDbiQ8CFG6.
PRIDEiQ8CFG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi300992.
KEGGirno:300992.
UCSCiRGD:631360. rat.

Organism-specific databases

CTDi9254.
RGDi631360. Cacna2d2.

Phylogenomic databases

eggNOGiKOG2353. Eukaryota.
ENOG410XPDX. LUCA.
HOGENOMiHOG000004860.
HOVERGENiHBG057779.
InParanoidiQ8CFG6.
KOiK04859.
PhylomeDBiQ8CFG6.

Enzyme and pathway databases

ReactomeiR-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.

Miscellaneous databases

PROiQ8CFG6.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCA2D2_RAT
AccessioniPrimary (citable) accession number: Q8CFG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds gabapentin, an antiepileptic drug.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.