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Protein

Voltage-dependent calcium channel subunit alpha-2/delta-2

Gene

Cacna2d2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis (By similarity).By similarity

Miscellaneous

Binds gabapentin, an antiepileptic drug.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi300Divalent metal cationBy similarity1
Metal bindingi302Divalent metal cationBy similarity1
Metal bindingi304Divalent metal cationBy similarity1

GO - Molecular functioni

  • calcium channel regulator activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  • calcium ion transport Source: RGD
  • regulation of ion transmembrane transport Source: UniProtKB-KW

Keywordsi

Molecular functionCalcium channel, Ion channel, Voltage-gated channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-2
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-2
Cleaved into the following 2 chains:
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631360 Cacna2d2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 1119ExtracellularSequence analysisAdd BLAST1101
Transmembranei1120 – 1140HelicalSequence analysisAdd BLAST21
Topological domaini1141 – 1157CytoplasmicSequence analysisAdd BLAST17

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000030464319 – 1157Voltage-dependent calcium channel subunit alpha-2/delta-2Add BLAST1139
ChainiPRO_000030464419 – 1004Voltage-dependent calcium channel subunit alpha-2-2Sequence analysisAdd BLAST986
ChainiPRO_00003046451005 – 1157Voltage-dependent calcium channel subunit delta-2Sequence analysisAdd BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi421N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi446 ↔ 1104Interchain (between alpha-2-2 and delta-2 chains)By similarity
Glycosylationi510N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi543N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi627N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi864N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity
May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8CFG6
PRIDEiQ8CFG6

Expressioni

Tissue specificityi

In heart, it is highly expressed in atrium and at lower level in ventricle.2 Publications

Interactioni

Subunit structurei

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Protein-protein interaction databases

BioGridi256785, 1 interactor
IntActiQ8CFG6, 3 interactors
STRINGi10116.ENSRNOP00000021216

Structurei

3D structure databases

ProteinModelPortaliQ8CFG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 472VWFAPROSITE-ProRule annotationAdd BLAST179
Domaini488 – 577CacheAdd BLAST90

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi300 – 304MIDAS-like motif5

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2353 Eukaryota
ENOG410XPDX LUCA
HOGENOMiHOG000004860
HOVERGENiHBG057779
InParanoidiQ8CFG6
KOiK04859
PhylomeDBiQ8CFG6

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013680 VDCC_a2/dsu
IPR013608 VWA_N
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF08473 VGCC_alpha2, 1 hit
PF00092 VWA, 1 hit
PF08399 VWA_N, 1 hit
SMARTiView protein in SMART
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50234 VWFA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPARTCGA SWPGPVRTAR PWPGRGPRPC PDPRGPASGP ARPLLLLLPP
60 70 80 90 100
LLLLPLLTAP GASAYSFPQQ HTMQHWARRL EQEIDGVMRI FGGVQQLREI
110 120 130 140 150
YKDNRNLFDV QENEPQKLVE KVAGDIESLL DRKVQALKRL ADAAENFQKA
160 170 180 190 200
HRWQDNIKEE DIMYYDAKAD AELDDPESED MERGSKTSAL RLDFIEEPNF
210 220 230 240 250
KNKVNYSYTA VQIPTDIYKG STVILNELNW TEALENVFIE NRRQDPTLLW
260 270 280 290 300
QVFGSATGVT RYYPATPWRA PKKIDLYDVR RRPWYIQGAS SPKDMVIIVD
310 320 330 340 350
VSGSVSGLTL KLMKTSVCEM LDTLSDDDYV NVASFNEKAQ PVSCFTHLVQ
360 370 380 390 400
ANVRNKKVFK EAVQGMVAKG TTGYKAGFEY AFDQLQNSNI TRANCNKMIM
410 420 430 440 450
MFTDGGEDRV QDVFEKYNWP NRTVRVFTFS VGQHNYDVTP LQWMACTNKG
460 470 480 490 500
YYFEIPSIGA IRINTQEYLD VLGRPMVLAG KDAKQVQWTN VYEDALGLGL
510 520 530 540 550
VVTGTLPVFN LTQDGPGDKK NQLILGVMGI DVALNDIKRL TPNYTLGANG
560 570 580 590 600
YVFAIDLNGY VLLHPNLKPQ ITNFREPVTL DFLDAELEDE NKEEIRRSMI
610 620 630 640 650
DGDKGHKQIR TLVKSLDERY IDEVIRNYTW VPIRSTNYSL GLVLPPYSTY
660 670 680 690 700
YLQANLSDQI LQVKLPISKL KDFEFLLPSS FESEGHVFIA PREYCKDLNA
710 720 730 740 750
SDNNTEFLKN FIELMEKVTP DSKQCNNFLL HNLILDTGIT QQLVERVWRD
760 770 780 790 800
QDLNTYSLLA VFAATDGGIT RVFPNKAAED WTENPEPFNA SFYRRSLDNR
810 820 830 840 850
GYIFKPPHQD SLLRPLELEN DTVGVLVSTA VELSLGRRTL RPAVVGVKLD
860 870 880 890 900
LEAWAEKFKV LASNRTHQDQ PQKQCGPSSH CEMDCEVNNE DLLCVLIDDG
910 920 930 940 950
GFLVLSNQNH QWDQVGRFFS EVDANLMLAL YNNSFYTRKE SYDYQAACAP
960 970 980 990 1000
QPPGNLGAAP RGVFVPTIAD FLNLAWWTSA AAWSLFQQLL YGLIYHSWFQ
1010 1020 1030 1040 1050
ADPAEAEGSP ETRESSCVMK QTQYYFGSVN ASYNAIIDCG NCSRLFHAQR
1060 1070 1080 1090 1100
LTNTNLLFVV AEKPLCSQCE VGRLLQKETH CPADGPEQCE LVQRPRYRTG
1110 1120 1130 1140 1150
PHICFDYNAT EDTSDCGRGA SFPPSLGVLV SLQLLLLLGL PPRPQPQIHS

FTPSRRL
Length:1,157
Mass (Da):130,776
Last modified:March 1, 2003 - v1
Checksum:i5E81446A1E1EBAF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486277 mRNA Translation: AAO14653.1
RefSeqiNP_783182.1, NM_175592.2
UniGeneiRn.91563

Genome annotation databases

GeneIDi300992
KEGGirno:300992
UCSCiRGD:631360 rat

Similar proteinsi

Entry informationi

Entry nameiCA2D2_RAT
AccessioniPrimary (citable) accession number: Q8CFG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health