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Q8CFG6 (CA2D2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-2
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-2

Cleaved into the following 2 chains:

  1. Voltage-dependent calcium channel subunit alpha-2-2
  2. Voltage-dependent calcium channel subunit delta-2
Gene names
Name:Cacna2d2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1157 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis By similarity.

Subunit structure

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein Potential. Note: Colocalizes with CACNA1A in lipid raft fractions By similarity.

Tissue specificity

In heart, it is highly expressed in atrium and at lower level in ventricle. Ref.1 Ref.2

Domain

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch By similarity.

Post-translational modification

N-glycosylated By similarity.

May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role By similarity.

Miscellaneous

Binds gabapentin, an antiepileptic drug By similarity.

Sequence similarities

Belongs to the calcium channel subunit alpha-2/delta family.

Contains 1 cache domain.

Contains 1 VWFA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 11571139Voltage-dependent calcium channel subunit alpha-2/delta-2
PRO_0000304643
Chain19 – 1004986Voltage-dependent calcium channel subunit alpha-2-2 Potential
PRO_0000304644
Chain1005 – 1157153Voltage-dependent calcium channel subunit delta-2 Potential
PRO_0000304645

Regions

Topological domain19 – 11191101Extracellular Potential
Transmembrane1120 – 114021Helical; Potential
Topological domain1141 – 115717Cytoplasmic Potential
Domain294 – 472179VWFA
Domain488 – 57790Cache
Motif300 – 3045MIDAS-like motif

Sites

Metal binding3001Divalent metal cation By similarity
Metal binding3021Divalent metal cation By similarity
Metal binding3041Divalent metal cation By similarity

Amino acid modifications

Glycosylation2051N-linked (GlcNAc...) Potential
Glycosylation3891N-linked (GlcNAc...) Potential
Glycosylation4211N-linked (GlcNAc...) Potential
Glycosylation5101N-linked (GlcNAc...) Potential
Glycosylation5431N-linked (GlcNAc...) Potential
Glycosylation6271N-linked (GlcNAc...) Potential
Glycosylation8641N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8CFG6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5E81446A1E1EBAF3

FASTA1,157130,776
        10         20         30         40         50         60 
MAVPARTCGA SWPGPVRTAR PWPGRGPRPC PDPRGPASGP ARPLLLLLPP LLLLPLLTAP 

        70         80         90        100        110        120 
GASAYSFPQQ HTMQHWARRL EQEIDGVMRI FGGVQQLREI YKDNRNLFDV QENEPQKLVE 

       130        140        150        160        170        180 
KVAGDIESLL DRKVQALKRL ADAAENFQKA HRWQDNIKEE DIMYYDAKAD AELDDPESED 

       190        200        210        220        230        240 
MERGSKTSAL RLDFIEEPNF KNKVNYSYTA VQIPTDIYKG STVILNELNW TEALENVFIE 

       250        260        270        280        290        300 
NRRQDPTLLW QVFGSATGVT RYYPATPWRA PKKIDLYDVR RRPWYIQGAS SPKDMVIIVD 

       310        320        330        340        350        360 
VSGSVSGLTL KLMKTSVCEM LDTLSDDDYV NVASFNEKAQ PVSCFTHLVQ ANVRNKKVFK 

       370        380        390        400        410        420 
EAVQGMVAKG TTGYKAGFEY AFDQLQNSNI TRANCNKMIM MFTDGGEDRV QDVFEKYNWP 

       430        440        450        460        470        480 
NRTVRVFTFS VGQHNYDVTP LQWMACTNKG YYFEIPSIGA IRINTQEYLD VLGRPMVLAG 

       490        500        510        520        530        540 
KDAKQVQWTN VYEDALGLGL VVTGTLPVFN LTQDGPGDKK NQLILGVMGI DVALNDIKRL 

       550        560        570        580        590        600 
TPNYTLGANG YVFAIDLNGY VLLHPNLKPQ ITNFREPVTL DFLDAELEDE NKEEIRRSMI 

       610        620        630        640        650        660 
DGDKGHKQIR TLVKSLDERY IDEVIRNYTW VPIRSTNYSL GLVLPPYSTY YLQANLSDQI 

       670        680        690        700        710        720 
LQVKLPISKL KDFEFLLPSS FESEGHVFIA PREYCKDLNA SDNNTEFLKN FIELMEKVTP 

       730        740        750        760        770        780 
DSKQCNNFLL HNLILDTGIT QQLVERVWRD QDLNTYSLLA VFAATDGGIT RVFPNKAAED 

       790        800        810        820        830        840 
WTENPEPFNA SFYRRSLDNR GYIFKPPHQD SLLRPLELEN DTVGVLVSTA VELSLGRRTL 

       850        860        870        880        890        900 
RPAVVGVKLD LEAWAEKFKV LASNRTHQDQ PQKQCGPSSH CEMDCEVNNE DLLCVLIDDG 

       910        920        930        940        950        960 
GFLVLSNQNH QWDQVGRFFS EVDANLMLAL YNNSFYTRKE SYDYQAACAP QPPGNLGAAP 

       970        980        990       1000       1010       1020 
RGVFVPTIAD FLNLAWWTSA AAWSLFQQLL YGLIYHSWFQ ADPAEAEGSP ETRESSCVMK 

      1030       1040       1050       1060       1070       1080 
QTQYYFGSVN ASYNAIIDCG NCSRLFHAQR LTNTNLLFVV AEKPLCSQCE VGRLLQKETH 

      1090       1100       1110       1120       1130       1140 
CPADGPEQCE LVQRPRYRTG PHICFDYNAT EDTSDCGRGA SFPPSLGVLV SLQLLLLLGL 

      1150 
PPRPQPQIHS FTPSRRL

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References

[1]"Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1."
Chu P.-J., Best P.M.
J. Mol. Cell. Cardiol. 35:207-215(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Heart atrium.
[2]"Molecular and electrophysiological differences in the L-type Ca2+ channel of the atrium and ventricle of rat hearts."
Hatano S., Yamashita T., Sekiguchi A., Iwasaki Y., Nakazawa K., Sagara K., Iinuma H., Aizawa T., Fu L.-T.
Jpn. Circ. J. 70:610-614(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF486277 mRNA. Translation: AAO14653.1.
IPIIPI00191089.
RefSeqNP_783182.1. NM_175592.2.
UniGeneRn.91563.

3D structure databases

ProteinModelPortalQ8CFG6.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8CFG6. 4 interactions.
STRING10116.ENSRNOP00000021216.

Proteomic databases

PaxDbQ8CFG6.
PRIDEQ8CFG6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID300992.
KEGGrno:300992.
UCSCRGD:631360. rat.

Organism-specific databases

CTD9254.
RGD631360. Cacna2d2.

Phylogenomic databases

eggNOGNOG307080.
HOGENOMHOG000004860.
HOVERGENHBG057779.
InParanoidQ8CFG6.
KOK04859.
OrthoDBEOG4FBHS3.

Enzyme and pathway databases

ReactomeREACT_113568. Metabolism.

Gene expression databases

GenevestigatorQ8CFG6.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamPF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTSM00327. VWA. 1 hit.
[Graphical view]
PROSITEPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBQ8CFG6.
ChEMBLCHEMBL3914.
NextBio647862.

Entry information

Entry nameCA2D2_RAT
AccessionPrimary (citable) accession number: Q8CFG6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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