Voltage-dependent calcium channel subunit alpha-2/delta-3 precursor

Preferred order of sections

Names and origin

Protein names

Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-3

Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-3

Cleaved into the following 2 chains:

Voltage-dependent calcium channel subunit alpha-2-3
Voltage-dependent calcium channel subunit delta-3

Gene names

Name:

Cacna2d3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G) By similarity.
Subunit structure	Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	In heart, it is expressed in atrium but not in ventricle. Ref.1
Induction	By IGF1. Ref.1
Domain	The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch By similarity.
Post-translational modification	N-glycosylated By similarity. May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role By similarity.
Miscellaneous	In contrast to CACNA2D1 and CACNA2D2, it does not bind gabapentin, an antiepileptic drug By similarity.
Sequence similarities	Belongs to the calcium channel subunit alpha-2/delta family. Contains 1 cache domain. Contains 1 VWFA domain.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Molecular function	Calcium channel Ion channel Voltage-gated channel
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	voltage-gated calcium channel complex Traceable author statement Ref.1. Source: RGD
Molecular_function	calcium channel regulator activity Traceable author statement Ref.1. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW voltage-gated calcium channel activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 33

33

Potential

Chain

34 – 1085

1052

Voltage-dependent calcium channel subunit alpha-2/delta-3

PRO_0000304652

Chain

34 – ?

Voltage-dependent calcium channel subunit alpha-2-3 Potential

PRO_0000304653

Chain

? – 1085

Voltage-dependent calcium channel subunit delta-3 Potential

PRO_0000304654

Regions

Topological domain

34 – 1001

968

Extracellular Potential

Transmembrane

1002 – 1024

23

Helical; Potential

Topological domain

1025 – 1085

61

Cytoplasmic Potential

Domain

256 – 438

183

VWFA

Domain

452 – 543

92

Cache

Motif

262 – 266

5

MIDAS-like motif

Sites

Metal binding

262

1

Divalent metal cation By similarity

Metal binding

264

1

Divalent metal cation By similarity

Metal binding

266

1

Divalent metal cation By similarity

Amino acid modifications

Glycosylation

166

1

N-linked (GlcNAc...) Potential

Glycosylation

309

1

N-linked (GlcNAc...) Potential

Glycosylation

547

1

N-linked (GlcNAc...) Potential

Glycosylation

626

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CFG5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 9584F533E318002A
Show »

FASTA

1,085

122,204

        10         20         30         40         50         60 
MAGPGSLCCA SRGASALLAT ALLYAALGDV VRSEQQIPLS VVKLWASAFG GEIKSIAAKY 

        70         80         90        100        110        120 
SGSQLLQKKY KEYEKDVAIE EIDGLQLVKK LAKNMEEMFH KKSEAVRRLV EAAEEAHLKH 

       130        140        150        160        170        180 
EFDADLQYEY FNAVLINERD KDGNFLELGK EFILAPNDHF NNLPVNISLS DVQVPTNMYN 

       190        200        210        220        230        240 
KDPAIVNGVY WSESLNKVFV DNFDRDPSLI WQYFGSAKGF FRQYPGIKWE PDENGVIAFD 

       250        260        270        280        290        300 
CRNRKWYIQA ATSPKDVVIL VDVSGSMKGL RLTIAKQTVS SILDTLGDDD FFNIITYNEE 

       310        320        330        340        350        360 
LHYVEPCLNG TLVQADRTNK EHFREHLDKL FAKGIGMLDI ALNEAFNVLS DFNHTGQGSI 

       370        380        390        400        410        420 
CSQAIMLITD GAVDTYDTIF AKYNWPERKV RIFTYLIGRE AAFADNLKWM ACANKGFFTQ 

       430        440        450        460        470        480 
ISTLADVQEN VMEYLHVLSR PKVIDQEHDV VWTEAYIDST LADDQGLVLM TTVAMPVFSK 

       490        500        510        520        530        540 
QNETRSKGIL LGVVGTDVPV KELLKTIPKY KLGIHGYAFA ITNNGYILTH PELRPLYEEG 

       550        560        570        580        590        600 
KKRRKPNYSS VDLSEVEWED RDDVLRNAMV NRKTGKFSME VKKTVDKGKR VLVMTNDYYY 

       610        620        630        640        650        660 
TDIKGAPFSL GVALSRGHGK YFFRGNVTIE EGLHDLEHPD VSLADEWSYC NTDLHPEHRH 

       670        680        690        700        710        720 
LSQLEAIKLY LKGKEPLLQC DKELIQEVLF DAVVSAPIEA YWTSLALNKS ENSDKGVEVA 

       730        740        750        760        770        780 
FLGTRTGLSR INLFVGAEQL TNQDFLKARD KENIFNADHF PLWYRRAAEQ IPGSFVYSIP 

       790        800        810        820        830        840 
FSTGTVNKSN VVTASTSIQL LDERKSPVVA AVGIQMKLEF FQRKFWTASR QCASLDGKCS 

       850        860        870        880        890        900 
ISCDDETVNC YLIDNNGFIL VSEDYTQTGD FFGEVEGAVM NKLLTMGSFK RITLYDYQAM 

       910        920        930        940        950        960 
CRANKESSDS AHGLLDPYKA FLSAAKWIVT ELVLFLVEFN LCSWWHSDMT AKAQKLKQTL 

       970        980        990       1000       1010       1020 
EPCDTEYPAF VSERTIKETT GNIACEDCSK SFVIQQIPSS NLFMVVVDSS CLCESVAPIT 

      1030       1040       1050       1060       1070       1080 
MAPIEIRYNE SLKCERLKAQ KIRRRPESCH GFHPEENARE CGGASSLQAQ VALLLLPLVS 


SLFSR

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References

[1]

"Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1."
Chu P.-J., Best P.M.
J. Mol. Cell. Cardiol. 35:207-215(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Heart atrium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF486278 mRNA. Translation: AAO14654.1.
IPI	IPI00191088.
RefSeq	NP_783185.1. NM_175595.2.
UniGene	Rn.229510.
3D structure databases
ProteinModelPortal	Q8CFG5.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000042602.
PTM databases
PhosphoSite	Q8CFG5.
Proteomic databases
PaxDb	Q8CFG5.
PRIDE	Q8CFG5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	306243.
KEGG	rno:306243.
UCSC	RGD:631361. rat.
Organism-specific databases
CTD	55799.
RGD	631361. Cacna2d3.
Phylogenomic databases
eggNOG	NOG307080.
HOGENOM	HOG000010247.
HOVERGEN	HBG107124.
KO	K04860.
Enzyme and pathway databases
Reactome	REACT_113568. Metabolism.
Gene expression databases
ArrayExpress	Q8CFG5.
Genevestigator	Q8CFG5.
Family and domain databases
InterPro	IPR004010. Cache_domain. IPR013608. VWA_N. IPR002035. VWF_A. [Graphical view]
Pfam	PF02743. Cache_1. 1 hit. PF08399. VWA_N. 1 hit. [Graphical view]
SMART	SM00327. VWA. 1 hit. [Graphical view]
PROSITE	PS50234. VWFA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8CFG5.
ChEMBL	CHEMBL3281.
NextBio	655665.

Entry information

Entry name

CA2D3_RAT

Accession

Primary (citable) accession number: Q8CFG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents