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Q8CFG5

- CA2D3_RAT

UniProt

Q8CFG5 - CA2D3_RAT

Protein

Voltage-dependent calcium channel subunit alpha-2/delta-3

Gene

Cacna2d3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G) By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi262 – 2621Divalent metal cationBy similarity
    Metal bindingi264 – 2641Divalent metal cationBy similarity
    Metal bindingi266 – 2661Divalent metal cationBy similarity

    GO - Molecular functioni

    1. calcium channel regulator activity Source: RGD
    2. metal ion binding Source: UniProtKB-KW
    3. voltage-gated calcium channel activity Source: RGD

    GO - Biological processi

    1. calcium ion transport Source: RGD

    Keywords - Molecular functioni

    Calcium channel, Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Voltage-dependent calcium channel subunit alpha-2/delta-3
    Alternative name(s):
    Voltage-gated calcium channel subunit alpha-2/delta-3
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Cacna2d3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi631361. Cacna2d3.

    Subcellular locationi

    GO - Cellular componenti

    1. voltage-gated calcium channel complex Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1085Voltage-dependent calcium channel subunit delta-3Sequence AnalysisPRO_0000304654
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 10851052Voltage-dependent calcium channel subunit alpha-2/delta-3PRO_0000304652Add
    BLAST
    Chaini34 – ?Voltage-dependent calcium channel subunit alpha-2-3Sequence AnalysisPRO_0000304653

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi412 ↔ 1049Interchain (between alpha-2-3 and delta-3 chains)By similarity
    Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
    Modified residuei918 – 9181Phosphotyrosine1 Publication

    Post-translational modificationi

    N-glycosylated.By similarity
    May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8CFG5.
    PRIDEiQ8CFG5.

    PTM databases

    PhosphoSiteiQ8CFG5.

    Expressioni

    Tissue specificityi

    In heart, it is expressed in atrium but not in ventricle.1 Publication

    Inductioni

    By IGF1.1 Publication

    Gene expression databases

    GenevestigatoriQ8CFG5.

    Interactioni

    Subunit structurei

    Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000042602.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFG5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini34 – 1001968ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1084 – 10852CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1063 – 108321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini256 – 438183VWFAPROSITE-ProRule annotationAdd
    BLAST
    Domaini452 – 54392CacheAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi262 – 2665MIDAS-like motif

    Domaini

    The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

    Sequence similaritiesi

    Contains 1 cache domain.Curated
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG307080.
    HOGENOMiHOG000010247.
    HOVERGENiHBG107124.
    KOiK04860.
    PhylomeDBiQ8CFG5.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR004010. Cache_domain.
    IPR013608. VWA_N.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF02743. Cache_1. 1 hit.
    PF08399. VWA_N. 1 hit.
    [Graphical view]
    SMARTiSM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    PROSITEiPS50234. VWFA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CFG5-1 [UniParc]FASTAAdd to Basket

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    MAGPGSLCCA SRGASALLAT ALLYAALGDV VRSEQQIPLS VVKLWASAFG     50
    GEIKSIAAKY SGSQLLQKKY KEYEKDVAIE EIDGLQLVKK LAKNMEEMFH 100
    KKSEAVRRLV EAAEEAHLKH EFDADLQYEY FNAVLINERD KDGNFLELGK 150
    EFILAPNDHF NNLPVNISLS DVQVPTNMYN KDPAIVNGVY WSESLNKVFV 200
    DNFDRDPSLI WQYFGSAKGF FRQYPGIKWE PDENGVIAFD CRNRKWYIQA 250
    ATSPKDVVIL VDVSGSMKGL RLTIAKQTVS SILDTLGDDD FFNIITYNEE 300
    LHYVEPCLNG TLVQADRTNK EHFREHLDKL FAKGIGMLDI ALNEAFNVLS 350
    DFNHTGQGSI CSQAIMLITD GAVDTYDTIF AKYNWPERKV RIFTYLIGRE 400
    AAFADNLKWM ACANKGFFTQ ISTLADVQEN VMEYLHVLSR PKVIDQEHDV 450
    VWTEAYIDST LADDQGLVLM TTVAMPVFSK QNETRSKGIL LGVVGTDVPV 500
    KELLKTIPKY KLGIHGYAFA ITNNGYILTH PELRPLYEEG KKRRKPNYSS 550
    VDLSEVEWED RDDVLRNAMV NRKTGKFSME VKKTVDKGKR VLVMTNDYYY 600
    TDIKGAPFSL GVALSRGHGK YFFRGNVTIE EGLHDLEHPD VSLADEWSYC 650
    NTDLHPEHRH LSQLEAIKLY LKGKEPLLQC DKELIQEVLF DAVVSAPIEA 700
    YWTSLALNKS ENSDKGVEVA FLGTRTGLSR INLFVGAEQL TNQDFLKARD 750
    KENIFNADHF PLWYRRAAEQ IPGSFVYSIP FSTGTVNKSN VVTASTSIQL 800
    LDERKSPVVA AVGIQMKLEF FQRKFWTASR QCASLDGKCS ISCDDETVNC 850
    YLIDNNGFIL VSEDYTQTGD FFGEVEGAVM NKLLTMGSFK RITLYDYQAM 900
    CRANKESSDS AHGLLDPYKA FLSAAKWIVT ELVLFLVEFN LCSWWHSDMT 950
    AKAQKLKQTL EPCDTEYPAF VSERTIKETT GNIACEDCSK SFVIQQIPSS 1000
    NLFMVVVDSS CLCESVAPIT MAPIEIRYNE SLKCERLKAQ KIRRRPESCH 1050
    GFHPEENARE CGGASSLQAQ VALLLLPLVS SLFSR 1085
    Length:1,085
    Mass (Da):122,204
    Last modified:March 1, 2003 - v1
    Checksum:i9584F533E318002A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486278 mRNA. Translation: AAO14654.1.
    RefSeqiNP_783185.1. NM_175595.2.
    UniGeneiRn.229510.

    Genome annotation databases

    GeneIDi306243.
    KEGGirno:306243.
    UCSCiRGD:631361. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486278 mRNA. Translation: AAO14654.1 .
    RefSeqi NP_783185.1. NM_175595.2.
    UniGenei Rn.229510.

    3D structure databases

    ProteinModelPortali Q8CFG5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000042602.

    Chemistry

    BindingDBi Q8CFG5.

    PTM databases

    PhosphoSitei Q8CFG5.

    Proteomic databases

    PaxDbi Q8CFG5.
    PRIDEi Q8CFG5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 306243.
    KEGGi rno:306243.
    UCSCi RGD:631361. rat.

    Organism-specific databases

    CTDi 55799.
    RGDi 631361. Cacna2d3.

    Phylogenomic databases

    eggNOGi NOG307080.
    HOGENOMi HOG000010247.
    HOVERGENi HBG107124.
    KOi K04860.
    PhylomeDBi Q8CFG5.

    Miscellaneous databases

    NextBioi 655665.
    PROi Q8CFG5.

    Gene expression databases

    Genevestigatori Q8CFG5.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR004010. Cache_domain.
    IPR013608. VWA_N.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF02743. Cache_1. 1 hit.
    PF08399. VWA_N. 1 hit.
    [Graphical view ]
    SMARTi SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    PROSITEi PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1."
      Chu P.-J., Best P.M.
      J. Mol. Cell. Cardiol. 35:207-215(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: Sprague-Dawley.
      Tissue: Heart atrium.
    2. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
      Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
      Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCA2D3_RAT
    AccessioniPrimary (citable) accession number: Q8CFG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to CACNA2D1 and CACNA2D2, it does not bind gabapentin, an antiepileptic drug.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3