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Protein

Voltage-dependent calcium channel subunit alpha-2/delta-3

Gene

Cacna2d3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi262Divalent metal cationBy similarity1
Metal bindingi264Divalent metal cationBy similarity1
Metal bindingi266Divalent metal cationBy similarity1

GO - Molecular functioni

  • calcium channel regulator activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  • calcium ion transport Source: RGD
  • regulation of calcium ion transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-3
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-3
Cleaved into the following 2 chains:
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631361. Cacna2d3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 1001ExtracellularSequence analysisAdd BLAST968
Transmembranei1063 – 1083HelicalSequence analysisAdd BLAST21
Topological domaini1084 – 1085CytoplasmicSequence analysis2

GO - Cellular componenti

  • voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000304654? – 1085Voltage-dependent calcium channel subunit delta-3Sequence analysis
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000030465234 – 1085Voltage-dependent calcium channel subunit alpha-2/delta-3Add BLAST1052
ChainiPRO_000030465334 – ?Voltage-dependent calcium channel subunit alpha-2-3Sequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi412 ↔ 1049Interchain (between alpha-2-3 and delta-3 chains)By similarity
Glycosylationi547N-linked (GlcNAc...)Sequence analysis1
Glycosylationi626N-linked (GlcNAc...)Sequence analysis1
Modified residuei918PhosphotyrosineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity
May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CFG5.
PRIDEiQ8CFG5.

PTM databases

iPTMnetiQ8CFG5.
PhosphoSitePlusiQ8CFG5.

Expressioni

Tissue specificityi

In heart, it is expressed in atrium but not in ventricle.1 Publication

Inductioni

By IGF1.1 Publication

Gene expression databases

BgeeiENSRNOG00000031287.

Interactioni

Subunit structurei

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042602.

Structurei

3D structure databases

ProteinModelPortaliQ8CFG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 438VWFAPROSITE-ProRule annotationAdd BLAST183
Domaini452 – 543CacheAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi262 – 266MIDAS-like motif5

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Contains 1 cache domain.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2353. Eukaryota.
ENOG410XPDX. LUCA.
HOGENOMiHOG000010247.
HOVERGENiHBG107124.
InParanoidiQ8CFG5.
KOiK04860.
PhylomeDBiQ8CFG5.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF08473. VGCC_alpha2. 1 hit.
PF13768. VWA_3. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPGSLCCA SRGASALLAT ALLYAALGDV VRSEQQIPLS VVKLWASAFG
60 70 80 90 100
GEIKSIAAKY SGSQLLQKKY KEYEKDVAIE EIDGLQLVKK LAKNMEEMFH
110 120 130 140 150
KKSEAVRRLV EAAEEAHLKH EFDADLQYEY FNAVLINERD KDGNFLELGK
160 170 180 190 200
EFILAPNDHF NNLPVNISLS DVQVPTNMYN KDPAIVNGVY WSESLNKVFV
210 220 230 240 250
DNFDRDPSLI WQYFGSAKGF FRQYPGIKWE PDENGVIAFD CRNRKWYIQA
260 270 280 290 300
ATSPKDVVIL VDVSGSMKGL RLTIAKQTVS SILDTLGDDD FFNIITYNEE
310 320 330 340 350
LHYVEPCLNG TLVQADRTNK EHFREHLDKL FAKGIGMLDI ALNEAFNVLS
360 370 380 390 400
DFNHTGQGSI CSQAIMLITD GAVDTYDTIF AKYNWPERKV RIFTYLIGRE
410 420 430 440 450
AAFADNLKWM ACANKGFFTQ ISTLADVQEN VMEYLHVLSR PKVIDQEHDV
460 470 480 490 500
VWTEAYIDST LADDQGLVLM TTVAMPVFSK QNETRSKGIL LGVVGTDVPV
510 520 530 540 550
KELLKTIPKY KLGIHGYAFA ITNNGYILTH PELRPLYEEG KKRRKPNYSS
560 570 580 590 600
VDLSEVEWED RDDVLRNAMV NRKTGKFSME VKKTVDKGKR VLVMTNDYYY
610 620 630 640 650
TDIKGAPFSL GVALSRGHGK YFFRGNVTIE EGLHDLEHPD VSLADEWSYC
660 670 680 690 700
NTDLHPEHRH LSQLEAIKLY LKGKEPLLQC DKELIQEVLF DAVVSAPIEA
710 720 730 740 750
YWTSLALNKS ENSDKGVEVA FLGTRTGLSR INLFVGAEQL TNQDFLKARD
760 770 780 790 800
KENIFNADHF PLWYRRAAEQ IPGSFVYSIP FSTGTVNKSN VVTASTSIQL
810 820 830 840 850
LDERKSPVVA AVGIQMKLEF FQRKFWTASR QCASLDGKCS ISCDDETVNC
860 870 880 890 900
YLIDNNGFIL VSEDYTQTGD FFGEVEGAVM NKLLTMGSFK RITLYDYQAM
910 920 930 940 950
CRANKESSDS AHGLLDPYKA FLSAAKWIVT ELVLFLVEFN LCSWWHSDMT
960 970 980 990 1000
AKAQKLKQTL EPCDTEYPAF VSERTIKETT GNIACEDCSK SFVIQQIPSS
1010 1020 1030 1040 1050
NLFMVVVDSS CLCESVAPIT MAPIEIRYNE SLKCERLKAQ KIRRRPESCH
1060 1070 1080
GFHPEENARE CGGASSLQAQ VALLLLPLVS SLFSR
Length:1,085
Mass (Da):122,204
Last modified:March 1, 2003 - v1
Checksum:i9584F533E318002A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486278 mRNA. Translation: AAO14654.1.
RefSeqiNP_783185.1. NM_175595.2.
UniGeneiRn.229510.

Genome annotation databases

GeneIDi306243.
KEGGirno:306243.
UCSCiRGD:631361. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486278 mRNA. Translation: AAO14654.1.
RefSeqiNP_783185.1. NM_175595.2.
UniGeneiRn.229510.

3D structure databases

ProteinModelPortaliQ8CFG5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042602.

PTM databases

iPTMnetiQ8CFG5.
PhosphoSitePlusiQ8CFG5.

Proteomic databases

PaxDbiQ8CFG5.
PRIDEiQ8CFG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi306243.
KEGGirno:306243.
UCSCiRGD:631361. rat.

Organism-specific databases

CTDi55799.
RGDi631361. Cacna2d3.

Phylogenomic databases

eggNOGiKOG2353. Eukaryota.
ENOG410XPDX. LUCA.
HOGENOMiHOG000010247.
HOVERGENiHBG107124.
InParanoidiQ8CFG5.
KOiK04860.
PhylomeDBiQ8CFG5.

Enzyme and pathway databases

ReactomeiR-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.

Miscellaneous databases

PROiQ8CFG5.

Gene expression databases

BgeeiENSRNOG00000031287.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF08473. VGCC_alpha2. 1 hit.
PF13768. VWA_3. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCA2D3_RAT
AccessioniPrimary (citable) accession number: Q8CFG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to CACNA2D1 and CACNA2D2, it does not bind gabapentin, an antiepileptic drug.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.