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Q8CFG5 (CA2D3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-3
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-3
Gene names
Name:Cacna2d3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1085 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G) By similarity.

Subunit structure

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

In heart, it is expressed in atrium but not in ventricle. Ref.1

Induction

By IGF1. Ref.1

Domain

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch By similarity.

Post-translational modification

N-glycosylated By similarity.

May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role By similarity.

Miscellaneous

In contrast to CACNA2D1 and CACNA2D2, it does not bind gabapentin, an antiepileptic drug By similarity.

Sequence similarities

Belongs to the calcium channel subunit alpha-2/delta family.

Contains 1 cache domain.

Contains 1 VWFA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 10851052Voltage-dependent calcium channel subunit alpha-2/delta-3
PRO_0000304652
Chain34 – ?Voltage-dependent calcium channel subunit alpha-2-3 PotentialPRO_0000304653
Chain? – 1085Voltage-dependent calcium channel subunit delta-3 PotentialPRO_0000304654

Regions

Topological domain34 – 1001968Extracellular Potential
Transmembrane1063 – 108321Helical; Potential
Topological domain1084 – 10852Cytoplasmic Potential
Domain256 – 438183VWFA
Domain452 – 54392Cache
Motif262 – 2665MIDAS-like motif

Sites

Metal binding2621Divalent metal cation By similarity
Metal binding2641Divalent metal cation By similarity
Metal binding2661Divalent metal cation By similarity

Amino acid modifications

Modified residue9181Phosphotyrosine Ref.2
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Glycosylation6261N-linked (GlcNAc...) Potential
Disulfide bond412 ↔ 1049Interchain (between alpha-2-3 and delta-3 chains) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CFG5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 9584F533E318002A

FASTA1,085122,204
        10         20         30         40         50         60 
MAGPGSLCCA SRGASALLAT ALLYAALGDV VRSEQQIPLS VVKLWASAFG GEIKSIAAKY 

        70         80         90        100        110        120 
SGSQLLQKKY KEYEKDVAIE EIDGLQLVKK LAKNMEEMFH KKSEAVRRLV EAAEEAHLKH 

       130        140        150        160        170        180 
EFDADLQYEY FNAVLINERD KDGNFLELGK EFILAPNDHF NNLPVNISLS DVQVPTNMYN 

       190        200        210        220        230        240 
KDPAIVNGVY WSESLNKVFV DNFDRDPSLI WQYFGSAKGF FRQYPGIKWE PDENGVIAFD 

       250        260        270        280        290        300 
CRNRKWYIQA ATSPKDVVIL VDVSGSMKGL RLTIAKQTVS SILDTLGDDD FFNIITYNEE 

       310        320        330        340        350        360 
LHYVEPCLNG TLVQADRTNK EHFREHLDKL FAKGIGMLDI ALNEAFNVLS DFNHTGQGSI 

       370        380        390        400        410        420 
CSQAIMLITD GAVDTYDTIF AKYNWPERKV RIFTYLIGRE AAFADNLKWM ACANKGFFTQ 

       430        440        450        460        470        480 
ISTLADVQEN VMEYLHVLSR PKVIDQEHDV VWTEAYIDST LADDQGLVLM TTVAMPVFSK 

       490        500        510        520        530        540 
QNETRSKGIL LGVVGTDVPV KELLKTIPKY KLGIHGYAFA ITNNGYILTH PELRPLYEEG 

       550        560        570        580        590        600 
KKRRKPNYSS VDLSEVEWED RDDVLRNAMV NRKTGKFSME VKKTVDKGKR VLVMTNDYYY 

       610        620        630        640        650        660 
TDIKGAPFSL GVALSRGHGK YFFRGNVTIE EGLHDLEHPD VSLADEWSYC NTDLHPEHRH 

       670        680        690        700        710        720 
LSQLEAIKLY LKGKEPLLQC DKELIQEVLF DAVVSAPIEA YWTSLALNKS ENSDKGVEVA 

       730        740        750        760        770        780 
FLGTRTGLSR INLFVGAEQL TNQDFLKARD KENIFNADHF PLWYRRAAEQ IPGSFVYSIP 

       790        800        810        820        830        840 
FSTGTVNKSN VVTASTSIQL LDERKSPVVA AVGIQMKLEF FQRKFWTASR QCASLDGKCS 

       850        860        870        880        890        900 
ISCDDETVNC YLIDNNGFIL VSEDYTQTGD FFGEVEGAVM NKLLTMGSFK RITLYDYQAM 

       910        920        930        940        950        960 
CRANKESSDS AHGLLDPYKA FLSAAKWIVT ELVLFLVEFN LCSWWHSDMT AKAQKLKQTL 

       970        980        990       1000       1010       1020 
EPCDTEYPAF VSERTIKETT GNIACEDCSK SFVIQQIPSS NLFMVVVDSS CLCESVAPIT 

      1030       1040       1050       1060       1070       1080 
MAPIEIRYNE SLKCERLKAQ KIRRRPESCH GFHPEENARE CGGASSLQAQ VALLLLPLVS 


SLFSR 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1."
Chu P.-J., Best P.M.
J. Mol. Cell. Cardiol. 35:207-215(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Heart atrium.
[2]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF486278 mRNA. Translation: AAO14654.1.
RefSeqNP_783185.1. NM_175595.2.
UniGeneRn.229510.

3D structure databases

ProteinModelPortalQ8CFG5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000042602.

Chemistry

BindingDBQ8CFG5.

PTM databases

PhosphoSiteQ8CFG5.

Proteomic databases

PaxDbQ8CFG5.
PRIDEQ8CFG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID306243.
KEGGrno:306243.
UCSCRGD:631361. rat.

Organism-specific databases

CTD55799.
RGD631361. Cacna2d3.

Phylogenomic databases

eggNOGNOG307080.
HOGENOMHOG000010247.
HOVERGENHBG107124.
KOK04860.
PhylomeDBQ8CFG5.

Enzyme and pathway databases

ReactomeREACT_205051. Metabolism.

Gene expression databases

GenevestigatorQ8CFG5.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR004010. Cache_domain.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamPF02743. Cache_1. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
PROSITEPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio655665.
PROQ8CFG5.

Entry information

Entry nameCA2D3_RAT
AccessionPrimary (citable) accession number: Q8CFG5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families