Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8CFE3

- RCOR1_MOUSE

UniProt

Q8CFE3 - RCOR1_MOUSE

Protein

REST corepressor 1

Gene

Rcor1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (07 Mar 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. protein binding Source: UniProtKB
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: Ensembl
    4. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. erythrocyte differentiation Source: UniProtKB
    2. histone H4 deacetylation Source: Ensembl
    3. positive regulation of megakaryocyte differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Chromatin regulator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    REST corepressor 1
    Alternative name(s):
    Protein CoREST
    Gene namesi
    Name:Rcor1
    Synonyms:D12Wsu95e, Kiaa0071
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:106340. Rcor1.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcriptional repressor complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477REST corepressor 1PRO_0000226774Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei118 – 1181PhosphoserineBy similarity
    Modified residuei251 – 2511Phosphoserine2 Publications
    Modified residuei451 – 4511PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8CFE3.
    PaxDbiQ8CFE3.
    PRIDEiQ8CFE3.

    PTM databases

    PhosphoSiteiQ8CFE3.

    Expressioni

    Tissue specificityi

    Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum and in Purkinje cells (at protein level).1 Publication

    Developmental stagei

    At embryonic day 8.5, it is highly expressed in the head mesenchyme, but neither in the somites nor in the presomitic mesoderm. By day 11.5 it is expressed fairly ubiquitously throughout the embryo.1 Publication

    Inductioni

    Down-regulated by the transcriptional repressor ZEB1 during NEUROD2-induced neurogenesis.1 Publication

    Gene expression databases

    ArrayExpressiQ8CFE3.
    BgeeiQ8CFE3.
    CleanExiMM_RCOR1.
    GenevestigatoriQ8CFE3.

    Interactioni

    Subunit structurei

    Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with REST. Interacts with the SMARCE1/BAF57, suggesting that the BHC complex may recruit the ATP-dependent chromatin-remodeling SWI-SNF complex By similarity. Interacts directly with GFI1 AND GFI1B in a RCOR/GFI/KDM1A/HDAC complex. Interacts with INMS1.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Nr4a2Q062195EBI-2337309,EBI-2337255

    Protein-protein interaction databases

    BioGridi229971. 1 interaction.
    DIPiDIP-48899N.
    IntActiQ8CFE3. 5 interactions.
    STRINGi10090.ENSMUSP00000112089.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFE3.
    SMRiQ8CFE3. Positions 94-230, 302-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini94 – 18087ELM2PROSITE-ProRule annotationAdd
    BLAST
    Domaini181 – 23252SANT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini372 – 42352SANT 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 248180Interaction with HDAC1By similarityAdd
    BLAST
    Regioni287 – 37589Interaction with KDM1ABy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili235 – 26228Sequence AnalysisAdd
    BLAST
    Coiled coili325 – 36036Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi18 – 6851Ala-richAdd
    BLAST

    Domaini

    The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A.By similarity

    Sequence similaritiesi

    Belongs to the CoREST family.Curated
    Contains 1 ELM2 domain.PROSITE-ProRule annotation
    Contains 2 SANT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG326181.
    GeneTreeiENSGT00730000110737.
    HOGENOMiHOG000065784.
    HOVERGENiHBG079800.
    InParanoidiQ8CFE3.
    KOiK11829.
    PhylomeDBiQ8CFE3.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR000949. ELM2_dom.
    IPR009057. Homeodomain-like.
    IPR001005. SANT/Myb.
    IPR017884. SANT_dom.
    [Graphical view]
    PfamiPF01448. ELM2. 1 hit.
    PF00249. Myb_DNA-binding. 1 hit.
    [Graphical view]
    SMARTiSM00717. SANT. 2 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 2 hits.
    PROSITEiPS51156. ELM2. 1 hit.
    PS51293. SANT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CFE3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEKGPEVSG KRRGRNTAAS AASAAASAAS AAASAAASAG TXSASAAAAA    50
    SAAAXPNNGQ NKSLAAAAPN GNSGSNSWEE GSSGSSSDEE HGGGGMRVGP 100
    QYQAAVPDFD PAKLARRSQE RDNLGMLVWS PNQSLSEAKL DEYIAIAKEK 150
    HGYNMEQALG MLFWHKHNIE KSLADLPNFT PFPDEWTVED KVLFEQAFSF 200
    HGKTFHRIQQ MLPDKSIASL VKFYYSWKKT RTKTSVMDRH ARKQKREREE 250
    SEDELEETNG SNPVDIEIDP NKESKKEVPP TETVPQVKKE KHSTQAKNRA 300
    KRKPPKGMFL SQEDVEAVSA NATAATTVLR QLDMELVSIK RQIQNIKQTN 350
    SALKEKLDGG IEPYRLPEVI QKCNARWTTE EQLLAVQAIR KYGRDFQAIS 400
    DVIGNKSVVQ VKNFFVNYRR RFNIDEVLQE WEAEHGKDET NGPANQKPVK 450
    SPESSIKIPE EEDEAASVLD VRYASAS 477
    Length:477
    Mass (Da):52,496
    Last modified:March 7, 2006 - v2
    Checksum:i80FBBF1FBE50D25B
    GO

    Sequence cautioni

    The sequence AAH42731.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti441 – 4411N → Y in BAC36804. (PubMed:16141072)Curated
    Sequence conflicti441 – 4411N → Y in BAE21612. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK077445 mRNA. Translation: BAC36804.1.
    AK133352 mRNA. Translation: BAE21612.1.
    BY729958 mRNA. No translation available.
    BC042731 mRNA. Translation: AAH42731.1. Different initiation.
    AB093210 mRNA. Translation: BAC41394.1.
    RefSeqiNP_932140.1. NM_198023.2.
    UniGeneiMm.23808.

    Genome annotation databases

    EnsembliENSMUST00000116388; ENSMUSP00000112089; ENSMUSG00000037896.
    GeneIDi217864.
    KEGGimmu:217864.
    UCSCiuc007pck.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK077445 mRNA. Translation: BAC36804.1 .
    AK133352 mRNA. Translation: BAE21612.1 .
    BY729958 mRNA. No translation available.
    BC042731 mRNA. Translation: AAH42731.1 . Different initiation.
    AB093210 mRNA. Translation: BAC41394.1 .
    RefSeqi NP_932140.1. NM_198023.2.
    UniGenei Mm.23808.

    3D structure databases

    ProteinModelPortali Q8CFE3.
    SMRi Q8CFE3. Positions 94-230, 302-434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229971. 1 interaction.
    DIPi DIP-48899N.
    IntActi Q8CFE3. 5 interactions.
    STRINGi 10090.ENSMUSP00000112089.

    PTM databases

    PhosphoSitei Q8CFE3.

    Proteomic databases

    MaxQBi Q8CFE3.
    PaxDbi Q8CFE3.
    PRIDEi Q8CFE3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000116388 ; ENSMUSP00000112089 ; ENSMUSG00000037896 .
    GeneIDi 217864.
    KEGGi mmu:217864.
    UCSCi uc007pck.1. mouse.

    Organism-specific databases

    CTDi 23186.
    MGIi MGI:106340. Rcor1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG326181.
    GeneTreei ENSGT00730000110737.
    HOGENOMi HOG000065784.
    HOVERGENi HBG079800.
    InParanoidi Q8CFE3.
    KOi K11829.
    PhylomeDBi Q8CFE3.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    ChiTaRSi RCOR1. mouse.
    NextBioi 376067.
    PROi Q8CFE3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CFE3.
    Bgeei Q8CFE3.
    CleanExi MM_RCOR1.
    Genevestigatori Q8CFE3.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR000949. ELM2_dom.
    IPR009057. Homeodomain-like.
    IPR001005. SANT/Myb.
    IPR017884. SANT_dom.
    [Graphical view ]
    Pfami PF01448. ELM2. 1 hit.
    PF00249. Myb_DNA-binding. 1 hit.
    [Graphical view ]
    SMARTi SM00717. SANT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 2 hits.
    PROSITEi PS51156. ELM2. 1 hit.
    PS51293. SANT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-111 AND 249-477.
      Strain: C57BL/6J.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-477.
      Strain: NMRI.
      Tissue: Mammary tumor.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
      DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 162-477.
      Tissue: Brain.
    4. "The co-repressor mSin3A is a functional component of the REST-CoREST repressor complex."
      Grimes J.A., Nielsen S.J., Battaglioli E., Miska E.A., Speh J.C., Berry D.L., Atouf F., Holdener B.C., Mandel G., Kouzarides T.
      J. Biol. Chem. 275:9461-9467(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis."
      Ballas N., Grunseich C., Lu D.D., Speh J.C., Mandel G.
      Cell 121:645-657(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1."
      Saleque S., Kim J., Rooke H.M., Orkin S.H.
      Mol. Cell 27:562-572(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY AS A COMPONENT OF A GFI-RCOR-KDM1A-HDAC COMPLEX, INTERACTION WITH GFI1 AND GFI1B, FUNCTION.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
      Ravanpay A.C., Hansen S.J., Olson J.M.
      Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    10. "Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors."
      Welcker J.E., Hernandez-Miranda L.R., Paul F.E., Jia S., Ivanov A., Selbach M., Birchmeier C.
      Development 140:4947-4958(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH INMS1.

    Entry informationi

    Entry nameiRCOR1_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFE3
    Secondary accession number(s): Q3V092, Q8BK28, Q8CHI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3