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Q8CFE3 (RCOR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
REST corepressor 1
Alternative name(s):
Protein CoREST
Gene names
Name:Rcor1
Synonyms:D12Wsu95e, Kiaa0071
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Ref.5 Ref.6 Ref.9

Subunit structure

Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with REST. Interacts with the SMARCE1/BAF57, suggesting that the BHC complex may recruit the ATP-dependent chromatin-remodeling SWI-SNF complex By similarity. Interacts directly with GFI1 AND GFI1B in a RCOR/GFI/KDM1A/HDAC complex. Interacts with INMS1. Ref.6 Ref.10

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum and in Purkinje cells (at protein level). Ref.9

Developmental stage

At embryonic day 8.5, it is highly expressed in the head mesenchyme, but neither in the somites nor in the presomitic mesoderm. By day 11.5 it is expressed fairly ubiquitously throughout the embryo. Ref.4

Induction

Down-regulated by the transcriptional repressor ZEB1 during NEUROD2-induced neurogenesis. Ref.9

Domain

The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A By similarity.

Sequence similarities

Belongs to the CoREST family.

Contains 1 ELM2 domain.

Contains 2 SANT domains.

Sequence caution

The sequence AAH42731.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Nr4a2Q062195EBI-2337309,EBI-2337255

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477REST corepressor 1
PRO_0000226774

Regions

Domain94 – 18087ELM2
Domain181 – 23252SANT 1
Domain372 – 42352SANT 2
Region69 – 248180Interaction with HDAC1 By similarity
Region287 – 37589Interaction with KDM1A By similarity
Coiled coil235 – 26228 Potential
Coiled coil325 – 36036 Potential
Compositional bias18 – 6851Ala-rich

Amino acid modifications

Modified residue1181Phosphoserine By similarity
Modified residue2511Phosphoserine Ref.7 Ref.8
Modified residue4511Phosphoserine By similarity

Experimental info

Sequence conflict4411N → Y in BAC36804. Ref.1
Sequence conflict4411N → Y in BAE21612. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8CFE3 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 80FBBF1FBE50D25B

FASTA47752,496
        10         20         30         40         50         60 
MVEKGPEVSG KRRGRNTAAS AASAAASAAS AAASAAASAG TXSASAAAAA SAAAXPNNGQ 

        70         80         90        100        110        120 
NKSLAAAAPN GNSGSNSWEE GSSGSSSDEE HGGGGMRVGP QYQAAVPDFD PAKLARRSQE 

       130        140        150        160        170        180 
RDNLGMLVWS PNQSLSEAKL DEYIAIAKEK HGYNMEQALG MLFWHKHNIE KSLADLPNFT 

       190        200        210        220        230        240 
PFPDEWTVED KVLFEQAFSF HGKTFHRIQQ MLPDKSIASL VKFYYSWKKT RTKTSVMDRH 

       250        260        270        280        290        300 
ARKQKREREE SEDELEETNG SNPVDIEIDP NKESKKEVPP TETVPQVKKE KHSTQAKNRA 

       310        320        330        340        350        360 
KRKPPKGMFL SQEDVEAVSA NATAATTVLR QLDMELVSIK RQIQNIKQTN SALKEKLDGG 

       370        380        390        400        410        420 
IEPYRLPEVI QKCNARWTTE EQLLAVQAIR KYGRDFQAIS DVIGNKSVVQ VKNFFVNYRR 

       430        440        450        460        470 
RFNIDEVLQE WEAEHGKDET NGPANQKPVK SPESSIKIPE EEDEAASVLD VRYASAS 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-111 AND 249-477.
Strain: C57BL/6J.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-477.
Strain: NMRI.
Tissue: Mammary tumor.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 162-477.
Tissue: Brain.
[4]"The co-repressor mSin3A is a functional component of the REST-CoREST repressor complex."
Grimes J.A., Nielsen S.J., Battaglioli E., Miska E.A., Speh J.C., Berry D.L., Atouf F., Holdener B.C., Mandel G., Kouzarides T.
J. Biol. Chem. 275:9461-9467(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis."
Ballas N., Grunseich C., Lu D.D., Speh J.C., Mandel G.
Cell 121:645-657(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1."
Saleque S., Kim J., Rooke H.M., Orkin S.H.
Mol. Cell 27:562-572(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY AS A COMPONENT OF A GFI-RCOR-KDM1A-HDAC COMPLEX, INTERACTION WITH GFI1 AND GFI1B, FUNCTION.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
Ravanpay A.C., Hansen S.J., Olson J.M.
Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[10]"Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors."
Welcker J.E., Hernandez-Miranda L.R., Paul F.E., Jia S., Ivanov A., Selbach M., Birchmeier C.
Development 140:4947-4958(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH INMS1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK077445 mRNA. Translation: BAC36804.1.
AK133352 mRNA. Translation: BAE21612.1.
BY729958 mRNA. No translation available.
BC042731 mRNA. Translation: AAH42731.1. Different initiation.
AB093210 mRNA. Translation: BAC41394.1.
RefSeqNP_932140.1. NM_198023.2.
UniGeneMm.23808.

3D structure databases

ProteinModelPortalQ8CFE3.
SMRQ8CFE3. Positions 94-230, 302-434.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229971. 1 interaction.
DIPDIP-48899N.
IntActQ8CFE3. 5 interactions.
STRING10090.ENSMUSP00000112089.

PTM databases

PhosphoSiteQ8CFE3.

Proteomic databases

MaxQBQ8CFE3.
PaxDbQ8CFE3.
PRIDEQ8CFE3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000116388; ENSMUSP00000112089; ENSMUSG00000037896.
GeneID217864.
KEGGmmu:217864.
UCSCuc007pck.1. mouse.

Organism-specific databases

CTD23186.
MGIMGI:106340. Rcor1.
RougeSearch...

Phylogenomic databases

eggNOGNOG326181.
GeneTreeENSGT00730000110737.
HOGENOMHOG000065784.
HOVERGENHBG079800.
InParanoidQ8CFE3.
KOK11829.
PhylomeDBQ8CFE3.

Gene expression databases

ArrayExpressQ8CFE3.
BgeeQ8CFE3.
CleanExMM_RCOR1.
GenevestigatorQ8CFE3.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR000949. ELM2_dom.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamPF01448. ELM2. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
[Graphical view]
SMARTSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMSSF46689. SSF46689. 2 hits.
PROSITEPS51156. ELM2. 1 hit.
PS51293. SANT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRCOR1. mouse.
NextBio376067.
PROQ8CFE3.
SOURCESearch...

Entry information

Entry nameRCOR1_MOUSE
AccessionPrimary (citable) accession number: Q8CFE3
Secondary accession number(s): Q3V092, Q8BK28, Q8CHI2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot