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Protein

REST corepressor 1

Gene

Rcor1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
REST corepressor 1
Alternative name(s):
Protein CoREST
Gene namesi
Name:Rcor1
Synonyms:D12Wsu95e, Kiaa0071
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:106340. Rcor1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002267741 – 480REST corepressor 1Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei121PhosphoserineBy similarity1
Modified residuei254PhosphoserineCombined sources1
Cross-linki291Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei454PhosphoserineCombined sources1
Cross-linki460Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CFE3.
MaxQBiQ8CFE3.
PaxDbiQ8CFE3.
PRIDEiQ8CFE3.

PTM databases

iPTMnetiQ8CFE3.
PhosphoSitePlusiQ8CFE3.

Expressioni

Tissue specificityi

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum and in Purkinje cells (at protein level).1 Publication

Developmental stagei

At embryonic day 8.5, it is highly expressed in the head mesenchyme, but neither in the somites nor in the presomitic mesoderm. By day 11.5 it is expressed fairly ubiquitously throughout the embryo.1 Publication

Inductioni

Down-regulated by the transcriptional repressor ZEB1 during NEUROD2-induced neurogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000037896.
CleanExiMM_RCOR1.

Interactioni

Subunit structurei

Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with REST. Interacts with the SMARCE1/BAF57, suggesting that the BHC complex may recruit the ATP-dependent chromatin-remodeling SWI-SNF complex (By similarity). Interacts directly with GFI1 AND GFI1B in a RCOR/GFI/KDM1A/HDAC complex. Interacts with INMS1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nr4a2Q062195EBI-2337309,EBI-2337255

GO - Molecular functioni

  • enzyme binding Source: MGI
  • transcription factor binding Source: GO_Central

Protein-protein interaction databases

BioGridi229971. 1 interactor.
CORUMiQ8CFE3.
DIPiDIP-48899N.
IntActiQ8CFE3. 5 interactors.
STRINGi10090.ENSMUSP00000112089.

Structurei

3D structure databases

ProteinModelPortaliQ8CFE3.
SMRiQ8CFE3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 183ELM2PROSITE-ProRule annotationAdd BLAST87
Domaini184 – 235SANT 1PROSITE-ProRule annotationAdd BLAST52
Domaini375 – 426SANT 2PROSITE-ProRule annotationAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 251Interaction with HDAC1By similarityAdd BLAST180
Regioni290 – 378Interaction with KDM1ABy similarityAdd BLAST89

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili238 – 265Sequence analysisAdd BLAST28
Coiled coili328 – 363Sequence analysisAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 71Ala-richAdd BLAST51

Domaini

The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A.By similarity

Sequence similaritiesi

Belongs to the CoREST family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1194. Eukaryota.
ENOG410XSIS. LUCA.
GeneTreeiENSGT00840000129748.
HOGENOMiHOG000065784.
HOVERGENiHBG079800.
InParanoidiQ8CFE3.
KOiK11829.
OMAiVWSPNQN.
OrthoDBiEOG091G096Y.
PhylomeDBiQ8CFE3.

Family and domain databases

InterProiView protein in InterPro
IPR000949. ELM2_dom.
IPR009057. Homeobox-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
PfamiView protein in Pfam
PF01448. ELM2. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
SMARTiView protein in SMART
SM01189. ELM2. 1 hit.
SM00717. SANT. 2 hits.
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiView protein in PROSITE
PS51156. ELM2. 1 hit.
PS51293. SANT. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8CFE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAMVEKGPE VSGKRRGRNT AASAASAAAS AASAAASAAA SAGTASASAA
60 70 80 90 100
AAASAAAAPN NGQNKSLAAA APNGNSGSNS WEEGSSGSSS DEEHGGGGMR
110 120 130 140 150
VGPQYQAAVP DFDPAKLARR SQERDNLGML VWSPNQSLSE AKLDEYIAIA
160 170 180 190 200
KEKHGYNMEQ ALGMLFWHKH NIEKSLADLP NFTPFPDEWT VEDKVLFEQA
210 220 230 240 250
FSFHGKTFHR IQQMLPDKSI ASLVKFYYSW KKTRTKTSVM DRHARKQKRE
260 270 280 290 300
REESEDELEE TNGSNPVDIE IDPNKESKKE VPPTETVPQV KKEKHSTQAK
310 320 330 340 350
NRAKRKPPKG MFLSQEDVEA VSANATAATT VLRQLDMELV SIKRQIQNIK
360 370 380 390 400
QTNSALKEKL DGGIEPYRLP EVIQKCNARW TTEEQLLAVQ AIRKYGRDFQ
410 420 430 440 450
AISDVIGNKS VVQVKNFFVN YRRRFNIDEV LQEWEAEHGK DETNGPANQK
460 470 480
PVKSPESSIK IPEEEDEAAS VLDVRYASAS
Length:480
Mass (Da):52,715
Last modified:February 15, 2017 - v3
Checksum:i7FA2AEA127EA297A
GO

Sequence cautioni

The sequence AAH42731 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti444N → Y in BAC36804 (PubMed:16141072).Curated1
Sequence conflicti444N → Y in BAE21612 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC122023 Genomic DNA. No translation available.
AC153152 Genomic DNA. No translation available.
AK077445 mRNA. Translation: BAC36804.1.
AK133352 mRNA. Translation: BAE21612.1.
BY729958 mRNA. No translation available.
BC042731 mRNA. Translation: AAH42731.1. Different initiation.
AB093210 mRNA. Translation: BAC41394.1.
RefSeqiNP_932140.1. NM_198023.2.
XP_006515823.1. XM_006515760.3.
UniGeneiMm.23808.

Genome annotation databases

EnsembliENSMUST00000084968; ENSMUSP00000082034; ENSMUSG00000037896.
GeneIDi217864.
KEGGimmu:217864.
UCSCiuc007pck.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiRCOR1_MOUSE
AccessioniPrimary (citable) accession number: Q8CFE3
Secondary accession number(s): K3W4P9
, Q3V092, Q8BK28, Q8CHI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: February 15, 2017
Last modified: September 27, 2017
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families