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Q8CFD5

- ERCC8_MOUSE

UniProt

Q8CFD5 - ERCC8_MOUSE

Protein

DNA excision repair protein ERCC-8

Gene

Ercc8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Involved in transcription-coupled nucleotide excision repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. It is the substrate-recognition component of the CSA complex (DCX(ERCC8) complex) which promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.By similarity

    Pathwayi

    GO - Molecular functioni

    1. ubiquitin-protein transferase activity Source: Ensembl

    GO - Biological processi

    1. positive regulation of DNA repair Source: Ensembl
    2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    3. protein autoubiquitination Source: Ensembl
    4. protein polyubiquitination Source: Ensembl
    5. response to oxidative stress Source: Ensembl
    6. response to UV Source: MGI
    7. response to X-ray Source: MGI
    8. transcription-coupled nucleotide-excision repair Source: Ensembl

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA excision repair protein ERCC-8
    Alternative name(s):
    Cockayne syndrome WD repeat protein CSA homolog
    Gene namesi
    Name:Ercc8
    Synonyms:Ckn1, Csa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1919241. Ercc8.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. Cul4A-RING E3 ubiquitin ligase complex Source: Ensembl
    2. nuclear matrix Source: Ensembl
    3. nucleotide-excision repair complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397DNA excision repair protein ERCC-8PRO_0000050971Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei391 – 3911PhosphoserineBy similarity
    Modified residuei392 – 3921PhosphoserineBy similarity
    Modified residuei393 – 3931PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8CFD5.

    PTM databases

    PhosphoSiteiQ8CFD5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CFD5.
    BgeeiQ8CFD5.
    GenevestigatoriQ8CFD5.

    Interactioni

    Subunit structurei

    Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Interacts with ERCC6 and KIAA1530/UVSSA. Interacts with a subunit of RNA polymerase II TFIIH. Interacts with DDB1.By similarity

    Protein-protein interaction databases

    BioGridi215079. 10 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFD5.
    SMRiQ8CFD5. Positions 1-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati41 – 8141WD 1Add
    BLAST
    Repeati97 – 13741WD 2Add
    BLAST
    Repeati184 – 22441WD 3Add
    BLAST
    Repeati243 – 28240WD 4Add
    BLAST
    Repeati332 – 37140WD 5Add
    BLAST

    Sequence similaritiesi

    Contains 5 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00390000009065.
    HOGENOMiHOG000248233.
    HOVERGENiHBG005409.
    InParanoidiQ8CFD5.
    KOiK10570.
    OMAiQELYSGG.
    OrthoDBiEOG7F511T.
    PhylomeDBiQ8CFD5.
    TreeFamiTF101237.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 5 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CFD5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSFLSARQS GLEDPLRLRR AQSTRRVLGL ELNKDRDVER IHGSGVNTLD    50
    IEPVEGRYML SGGSDGVVVL YDLENASRQP HYTCKAVCSV GRSHPDVHKY 100
    SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQAADVFNF EETVYSHHMS 150
    PAATKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRHDY 200
    ILATASADSR VKLWDVRRAS GCLLTLDQHN GKKSQAAESA NTAHNGKVNG 250
    LCFTSDGLHL LTIGTDNRMR LWNSSSGDNT LVNYGKVCND SRKGLQFAVS 300
    CGCSSEFVFV PHGSTIAVYA VHSGERLAML KGHYKSVDCC VFQPNFQELY 350
    SGSRDCNILA WVPPSYEPVP DDDDEAPAKS QLNPAFADAW SSSDEDG 397
    Length:397
    Mass (Da):43,689
    Last modified:June 7, 2005 - v2
    Checksum:i1B35EA61A00A611B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901V → I in AAH37200. (PubMed:15489334)Curated
    Sequence conflicti292 – 2921R → M in BAB27039. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ427222 mRNA. Translation: CAD20255.1.
    AK010576 mRNA. Translation: BAB27039.1.
    AK157175 mRNA. Translation: BAE33989.1.
    BC037200 mRNA. Translation: AAH37200.1.
    CCDSiCCDS26761.1.
    RefSeqiNP_082318.2. NM_028042.3.
    UniGeneiMm.212208.

    Genome annotation databases

    EnsembliENSMUST00000054835; ENSMUSP00000059211; ENSMUSG00000021694.
    GeneIDi71991.
    KEGGimmu:71991.
    UCSCiuc007rux.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ427222 mRNA. Translation: CAD20255.1 .
    AK010576 mRNA. Translation: BAB27039.1 .
    AK157175 mRNA. Translation: BAE33989.1 .
    BC037200 mRNA. Translation: AAH37200.1 .
    CCDSi CCDS26761.1.
    RefSeqi NP_082318.2. NM_028042.3.
    UniGenei Mm.212208.

    3D structure databases

    ProteinModelPortali Q8CFD5.
    SMRi Q8CFD5. Positions 1-363.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215079. 10 interactions.

    PTM databases

    PhosphoSitei Q8CFD5.

    Proteomic databases

    PRIDEi Q8CFD5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054835 ; ENSMUSP00000059211 ; ENSMUSG00000021694 .
    GeneIDi 71991.
    KEGGi mmu:71991.
    UCSCi uc007rux.1. mouse.

    Organism-specific databases

    CTDi 1161.
    MGIi MGI:1919241. Ercc8.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00390000009065.
    HOGENOMi HOG000248233.
    HOVERGENi HBG005409.
    InParanoidi Q8CFD5.
    KOi K10570.
    OMAi QELYSGG.
    OrthoDBi EOG7F511T.
    PhylomeDBi Q8CFD5.
    TreeFami TF101237.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.

    Miscellaneous databases

    NextBioi 335154.
    PROi Q8CFD5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CFD5.
    Bgeei Q8CFD5.
    Genevestigatori Q8CFD5.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 5 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Spleen.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiERCC8_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFD5
    Secondary accession number(s): Q3U066, Q8VDC4, Q9CWK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Ercc8 deficient mice develop normally, but show a loss of retinal photoreceptors after 4 months of age, and enhanced UV-sensitivity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3