Q8CFC7 (CLASR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CLK4-associating serine/arginine rich protein

Alternative name(s):
Clk4-associating SR-related protein
Serine/arginine-rich splicing factor 16
Splicing factor, arginine/serine-rich 16
Suppressor of white-apricot homolog 2

Gene names

Name:	Clasrp
Synonyms:	Clasp, Sfrs16, Srsf16, Swap2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably functions as an alternative splicing regulator. May regulate the mRNA splicing of genes such as CLK1. May act by regulating members of the CLK kinase family.
Subunit structure	Probably interacts with CLK4.
Subcellular location	Nucleus. Note: Located in nuclear dots. Isoform 2: Nucleus › nucleoplasm.
Tissue specificity	Highly expressed in brain. Expressed at intermediate level in lung and liver. In brain, it is expressed in the hippocampus, cerebellum and olfactory bulb.
Post-translational modification	Phosphorylated in vitro by CLK4.
Sequence similarities	Belongs to the splicing factor SR family.
Caution	It is uncertain whether Met-1 or Met-16 is the initiator.
Sequence caution	The sequence AAC82338.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB26225.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CFC7-1)

Also known as: L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CFC7-2)

Also known as: S;

The sequence of this isoform differs from the canonical sequence as follows:
     564-588: PKLTPQERLKLRMQKALNRQFKADK → VTQADPTGEAEASDAEGSEPPVQGG
     589-668: Missing.

Isoform 3 (identifier: Q8CFC7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     564-627: PKLTPQERLK...MKERERREKE → VSKNLELARL...TPAGPQVPDS
     628-668: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	CLK4-associating serine/arginine rich protein		PRO_0000081946
Regions
	Coiled coil	579 – 641	63	Potential
	Compositional bias	369 – 667	299	Arg-rich
	Compositional bias	377 – 529	153	Ser-rich
Amino acid modifications
	Modified residue	285	1	Phosphoserine By similarity
	Modified residue	294	1	Phosphoserine Ref.5
	Modified residue	327	1	Phosphothreonine By similarity
	Modified residue	331	1	Phosphoserine By similarity
	Modified residue	335	1	Phosphoserine By similarity
	Modified residue	541	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	564 – 627	64	PKLTP…RREKE → VSKNLELARLTPVSSSVQSW ARWACRGPRGRGEPAQRLGK GYPGKLSPIPASHGTPAGPQ VPDS in isoform 3.		VSP_008213
	Alternative sequence	564 – 588	25	PKLTP…FKADK → VTQADPTGEAEASDAEGSEP PVQGG in isoform 2.		VSP_008211
	Alternative sequence	589 – 668	80	Missing in isoform 2.		VSP_008212
	Alternative sequence	628 – 668	41	Missing in isoform 3.		VSP_008214

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (L) [UniParc]. Last modified April 14, 2009. Version 3. Checksum: 1A0322702F7A9C40	FASTA	668	76,825
	10 20 30 40 50 60 MWHEARKHER KLRGMMVDYK KRAERRREYY EKIKKDPAQF LQVHGRACKV HLDSAVALAA 70 80 90 100 110 120 ESPVNMMPWQ GDTNNMIDRF DVRAHLDHIP DYTPPLLTTI SPEQESDERK CNYERYRGLV 130 140 150 160 170 180 QNDFAGISEE QCLYQIYIDE LYGGLQRPSE DEKKKLAEKK ASIGYTYEDS TVAEVEKVAE 190 200 210 220 230 240 KPEEEESPAE EESNSDEDEV IPDIDVEVDV DELNQEQVAD LNKQATTYGM ADGDFVRMLR 250 260 270 280 290 300 KDKEEAEAIK HAKALEEEKA MYSGRRSRRQ RREFREKRLR GRKISPPSYA RRDSPTYDPY 310 320 330 340 350 360 KRSPSESSSE SRSRSRSPSP GREEKITFIT SFGGSDEEAA AAAAAAAASG AAPGKPPAPP 370 380 390 400 410 420 QTGGPAPGRN ASTRRRSSSS SASRTSSSRS SSRSSSRSRR GYYRSGRHAR SRSRSWSRSR 430 440 450 460 470 480 SRSRRYSRSR SRGRRHSDGG SRDGHRYSRS PARRGGYVPR RRSRSRSRSG DRYKRGARGP 490 500 510 520 530 540 RHHSSSHSRS SWSLSPSRSR SVTRSGSRSQ SRSRSRSQSH SQSQSHSPSP PREKLTRPAA 550 560 570 580 590 600 SPAVGEKLKK TEPAAGKETG AAKPKLTPQE RLKLRMQKAL NRQFKADKKA AQEKMIQQEH 610 620 630 640 650 660 ERQEREDELR AMARKIRMKE RERREKEREE WERQYSRQSR SPSPRYSREY SSSRRRSRSR SRSPHYRH
	Isoform 2 (S) [UniParc]. Checksum: 6085DFC8C8F7FFEE	FASTA	588	65,969
	10 20 30 40 50 60 MWHEARKHER KLRGMMVDYK KRAERRREYY EKIKKDPAQF LQVHGRACKV HLDSAVALAA 70 80 90 100 110 120 ESPVNMMPWQ GDTNNMIDRF DVRAHLDHIP DYTPPLLTTI SPEQESDERK CNYERYRGLV 130 140 150 160 170 180 QNDFAGISEE QCLYQIYIDE LYGGLQRPSE DEKKKLAEKK ASIGYTYEDS TVAEVEKVAE 190 200 210 220 230 240 KPEEEESPAE EESNSDEDEV IPDIDVEVDV DELNQEQVAD LNKQATTYGM ADGDFVRMLR 250 260 270 280 290 300 KDKEEAEAIK HAKALEEEKA MYSGRRSRRQ RREFREKRLR GRKISPPSYA RRDSPTYDPY 310 320 330 340 350 360 KRSPSESSSE SRSRSRSPSP GREEKITFIT SFGGSDEEAA AAAAAAAASG AAPGKPPAPP 370 380 390 400 410 420 QTGGPAPGRN ASTRRRSSSS SASRTSSSRS SSRSSSRSRR GYYRSGRHAR SRSRSWSRSR 430 440 450 460 470 480 SRSRRYSRSR SRGRRHSDGG SRDGHRYSRS PARRGGYVPR RRSRSRSRSG DRYKRGARGP 490 500 510 520 530 540 RHHSSSHSRS SWSLSPSRSR SVTRSGSRSQ SRSRSRSQSH SQSQSHSPSP PREKLTRPAA 550 560 570 580 SPAVGEKLKK TEPAAGKETG AAKVTQADPT GEAEASDAEG SEPPVQGG
	Isoform 3 [UniParc]. Checksum: 2656F106CBD80607	FASTA	627	70,289
	10 20 30 40 50 60 MWHEARKHER KLRGMMVDYK KRAERRREYY EKIKKDPAQF LQVHGRACKV HLDSAVALAA 70 80 90 100 110 120 ESPVNMMPWQ GDTNNMIDRF DVRAHLDHIP DYTPPLLTTI SPEQESDERK CNYERYRGLV 130 140 150 160 170 180 QNDFAGISEE QCLYQIYIDE LYGGLQRPSE DEKKKLAEKK ASIGYTYEDS TVAEVEKVAE 190 200 210 220 230 240 KPEEEESPAE EESNSDEDEV IPDIDVEVDV DELNQEQVAD LNKQATTYGM ADGDFVRMLR 250 260 270 280 290 300 KDKEEAEAIK HAKALEEEKA MYSGRRSRRQ RREFREKRLR GRKISPPSYA RRDSPTYDPY 310 320 330 340 350 360 KRSPSESSSE SRSRSRSPSP GREEKITFIT SFGGSDEEAA AAAAAAAASG AAPGKPPAPP 370 380 390 400 410 420 QTGGPAPGRN ASTRRRSSSS SASRTSSSRS SSRSSSRSRR GYYRSGRHAR SRSRSWSRSR 430 440 450 460 470 480 SRSRRYSRSR SRGRRHSDGG SRDGHRYSRS PARRGGYVPR RRSRSRSRSG DRYKRGARGP 490 500 510 520 530 540 RHHSSSHSRS SWSLSPSRSR SVTRSGSRSQ SRSRSRSQSH SQSQSHSPSP PREKLTRPAA 550 560 570 580 590 600 SPAVGEKLKK TEPAAGKETG AAKVSKNLEL ARLTPVSSSV QSWARWACRG PRGRGEPAQR 610 620 LGKGYPGKLS PIPASHGTPA GPQVPDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A transcriptional map in the region of 19q13 derived using direct sequencing and exon trapping." Yoshiura K., Murray J.C. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Tongue.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[4]	"Novel SR-rich-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk." Katsu R., Onogi H., Wada K., Kawaguchi Y., Hagiwara M. J. Biol. Chem. 277:44220-44228(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-668 (ISOFORMS 1 AND 2). Strain: BALB/c. Tissue: Brain.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042799 mRNA. Translation: AAC82338.1. Different initiation. AK009334 mRNA. Translation: BAB26225.1. Different initiation. BC051916 mRNA. Translation: AAH51916.2. AB080582 mRNA. Translation: BAC15600.1. AB080583 mRNA. Translation: BAC15601.1.
IPI	IPI00131244. IPI00172022. IPI00229282.
RefSeq	NP_057889.3. NM_016680.4.
UniGene	Mm.20913.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8CFC7.
Proteomic databases
PaxDb	Q8CFC7.
PRIDE	Q8CFC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000086041; ENSMUSP00000083205; ENSMUSG00000061028.
GeneID	53609.
KEGG	mmu:53609.
UCSC	uc009fml.1. mouse.
Organism-specific databases
CTD	11129.
MGI	MGI:1855695. Clasrp.
Phylogenomic databases
eggNOG	NOG316380.
GeneTree	ENSGT00700000104518.
HOVERGEN	HBG065901.
InParanoid	Q5RKW4.
KO	K13168.
OMA	HQLYLEE.
OrthoDB	EOG41VK3T.
Gene expression databases
Bgee	Q8CFC7.
Genevestigator	Q8CFC7.
GermOnline	ENSMUSG00000061028. Mus musculus.
Family and domain databases
InterPro	IPR019147. SWAP_N_domain. [Graphical view]
Pfam	PF09750. DRY_EERY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CLASRP. mouse.
NextBio	310315.
SOURCE	Search...

Entry information

Entry name

CLASR_MOUSE

Accession

Primary (citable) accession number: Q8CFC7
Secondary accession number(s): Q5RKW4, Q8CFC8, Q9Z2N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	April 14, 2009
Last modified:	April 3, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program