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Protein

CLK4-associating serine/arginine rich protein

Gene

Clasrp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably functions as an alternative splicing regulator. May regulate the mRNA splicing of genes such as CLK1. May act by regulating members of the CLK kinase family.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
CLK4-associating serine/arginine rich protein
Alternative name(s):
Clk4-associating SR-related protein
Serine/arginine-rich splicing factor 16
Splicing factor, arginine/serine-rich 16
Suppressor of white-apricot homolog 2
Gene namesi
Name:Clasrp
Synonyms:Clasp, Sfrs16, Srsf16, Swap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1855695. Clasrp.

Subcellular locationi

  • Nucleus

  • Note: Located in nuclear dots.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 668668CLK4-associating serine/arginine rich proteinPRO_0000081946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei285 – 2851PhosphoserineCombined sources
Modified residuei294 – 2941PhosphoserineBy similarity
Modified residuei327 – 3271PhosphothreonineBy similarity
Modified residuei331 – 3311PhosphoserineBy similarity
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei541 – 5411PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated in vitro by CLK4.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CFC7.
MaxQBiQ8CFC7.
PaxDbiQ8CFC7.
PRIDEiQ8CFC7.

PTM databases

iPTMnetiQ8CFC7.
PhosphoSiteiQ8CFC7.

Expressioni

Tissue specificityi

Highly expressed in brain. Expressed at intermediate level in lung and liver. In brain, it is expressed in the hippocampus, cerebellum and olfactory bulb.

Gene expression databases

BgeeiQ8CFC7.
GenevisibleiQ8CFC7. MM.

Interactioni

Subunit structurei

Probably interacts with CLK4.

Protein-protein interaction databases

BioGridi207329. 1 interaction.
STRINGi10090.ENSMUSP00000083205.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili579 – 64163Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi369 – 667299Arg-richAdd
BLAST
Compositional biasi377 – 529153Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2548. Eukaryota.
COG0303. LUCA.
GeneTreeiENSGT00730000111260.
HOVERGENiHBG065901.
InParanoidiQ8CFC7.
KOiK13168.
OMAiHSGDRYR.
OrthoDBiEOG7G7KPW.
PhylomeDBiQ8CFC7.
TreeFamiTF351621.

Family and domain databases

InterProiIPR019147. SWAP_N_domain.
[Graphical view]
PfamiPF09750. DRY_EERY. 1 hit.
[Graphical view]
SMARTiSM01141. DRY_EERY. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CFC7-1) [UniParc]FASTAAdd to basket

Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWHEARKHER KLRGMMVDYK KRAERRREYY EKIKKDPAQF LQVHGRACKV
60 70 80 90 100
HLDSAVALAA ESPVNMMPWQ GDTNNMIDRF DVRAHLDHIP DYTPPLLTTI
110 120 130 140 150
SPEQESDERK CNYERYRGLV QNDFAGISEE QCLYQIYIDE LYGGLQRPSE
160 170 180 190 200
DEKKKLAEKK ASIGYTYEDS TVAEVEKVAE KPEEEESPAE EESNSDEDEV
210 220 230 240 250
IPDIDVEVDV DELNQEQVAD LNKQATTYGM ADGDFVRMLR KDKEEAEAIK
260 270 280 290 300
HAKALEEEKA MYSGRRSRRQ RREFREKRLR GRKISPPSYA RRDSPTYDPY
310 320 330 340 350
KRSPSESSSE SRSRSRSPSP GREEKITFIT SFGGSDEEAA AAAAAAAASG
360 370 380 390 400
AAPGKPPAPP QTGGPAPGRN ASTRRRSSSS SASRTSSSRS SSRSSSRSRR
410 420 430 440 450
GYYRSGRHAR SRSRSWSRSR SRSRRYSRSR SRGRRHSDGG SRDGHRYSRS
460 470 480 490 500
PARRGGYVPR RRSRSRSRSG DRYKRGARGP RHHSSSHSRS SWSLSPSRSR
510 520 530 540 550
SVTRSGSRSQ SRSRSRSQSH SQSQSHSPSP PREKLTRPAA SPAVGEKLKK
560 570 580 590 600
TEPAAGKETG AAKPKLTPQE RLKLRMQKAL NRQFKADKKA AQEKMIQQEH
610 620 630 640 650
ERQEREDELR AMARKIRMKE RERREKEREE WERQYSRQSR SPSPRYSREY
660
SSSRRRSRSR SRSPHYRH
Length:668
Mass (Da):76,825
Last modified:April 14, 2009 - v3
Checksum:i1A0322702F7A9C40
GO
Isoform 2 (identifier: Q8CFC7-2) [UniParc]FASTAAdd to basket

Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     564-588: PKLTPQERLKLRMQKALNRQFKADK → VTQADPTGEAEASDAEGSEPPVQGG
     589-668: Missing.

Show »
Length:588
Mass (Da):65,969
Checksum:i6085DFC8C8F7FFEE
GO
Isoform 3 (identifier: Q8CFC7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     564-627: PKLTPQERLK...MKERERREKE → VSKNLELARL...TPAGPQVPDS
     628-668: Missing.

Show »
Length:627
Mass (Da):70,289
Checksum:i2656F106CBD80607
GO

Sequence cautioni

The sequence AAC82338.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB26225.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei564 – 62764PKLTP…RREKE → VSKNLELARLTPVSSSVQSW ARWACRGPRGRGEPAQRLGK GYPGKLSPIPASHGTPAGPQ VPDS in isoform 3. 2 PublicationsVSP_008213Add
BLAST
Alternative sequencei564 – 58825PKLTP…FKADK → VTQADPTGEAEASDAEGSEP PVQGG in isoform 2. 2 PublicationsVSP_008211Add
BLAST
Alternative sequencei589 – 66880Missing in isoform 2. 2 PublicationsVSP_008212Add
BLAST
Alternative sequencei628 – 66841Missing in isoform 3. 2 PublicationsVSP_008214Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042799 mRNA. Translation: AAC82338.1. Different initiation.
AK009334 mRNA. Translation: BAB26225.1. Different initiation.
BC051916 mRNA. Translation: AAH51916.2.
AB080582 mRNA. Translation: BAC15600.1.
AB080583 mRNA. Translation: BAC15601.1.
CCDSiCCDS52061.1. [Q8CFC7-1]
RefSeqiNP_057889.3. NM_016680.5. [Q8CFC7-1]
UniGeneiMm.20913.

Genome annotation databases

EnsembliENSMUST00000086041; ENSMUSP00000083205; ENSMUSG00000061028. [Q8CFC7-1]
ENSMUST00000207907; ENSMUSP00000146982; ENSMUSG00000061028. [Q8CFC7-3]
ENSMUST00000208068; ENSMUSP00000147103; ENSMUSG00000061028. [Q8CFC7-2]
GeneIDi53609.
KEGGimmu:53609.
UCSCiuc009fml.2. mouse. [Q8CFC7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042799 mRNA. Translation: AAC82338.1. Different initiation.
AK009334 mRNA. Translation: BAB26225.1. Different initiation.
BC051916 mRNA. Translation: AAH51916.2.
AB080582 mRNA. Translation: BAC15600.1.
AB080583 mRNA. Translation: BAC15601.1.
CCDSiCCDS52061.1. [Q8CFC7-1]
RefSeqiNP_057889.3. NM_016680.5. [Q8CFC7-1]
UniGeneiMm.20913.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207329. 1 interaction.
STRINGi10090.ENSMUSP00000083205.

PTM databases

iPTMnetiQ8CFC7.
PhosphoSiteiQ8CFC7.

Proteomic databases

EPDiQ8CFC7.
MaxQBiQ8CFC7.
PaxDbiQ8CFC7.
PRIDEiQ8CFC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086041; ENSMUSP00000083205; ENSMUSG00000061028. [Q8CFC7-1]
ENSMUST00000207907; ENSMUSP00000146982; ENSMUSG00000061028. [Q8CFC7-3]
ENSMUST00000208068; ENSMUSP00000147103; ENSMUSG00000061028. [Q8CFC7-2]
GeneIDi53609.
KEGGimmu:53609.
UCSCiuc009fml.2. mouse. [Q8CFC7-1]

Organism-specific databases

CTDi11129.
MGIiMGI:1855695. Clasrp.

Phylogenomic databases

eggNOGiKOG2548. Eukaryota.
COG0303. LUCA.
GeneTreeiENSGT00730000111260.
HOVERGENiHBG065901.
InParanoidiQ8CFC7.
KOiK13168.
OMAiHSGDRYR.
OrthoDBiEOG7G7KPW.
PhylomeDBiQ8CFC7.
TreeFamiTF351621.

Miscellaneous databases

ChiTaRSiClasrp. mouse.
PROiQ8CFC7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CFC7.
GenevisibleiQ8CFC7. MM.

Family and domain databases

InterProiIPR019147. SWAP_N_domain.
[Graphical view]
PfamiPF09750. DRY_EERY. 1 hit.
[Graphical view]
SMARTiSM01141. DRY_EERY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A transcriptional map in the region of 19q13 derived using direct sequencing and exon trapping."
    Yoshiura K., Murray J.C.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Tongue.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Novel SR-rich-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk."
    Katsu R., Onogi H., Wada K., Kawaguchi Y., Hagiwara M.
    J. Biol. Chem. 277:44220-44228(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-668 (ISOFORMS 1 AND 2).
    Strain: BALB/cJ.
    Tissue: Brain.
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-541, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiCLASR_MOUSE
AccessioniPrimary (citable) accession number: Q8CFC7
Secondary accession number(s): Q5RKW4, Q8CFC8, Q9Z2N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: April 14, 2009
Last modified: June 8, 2016
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-16 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.