Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8CFC4

- BGAT2_RAT

UniProt

Q8CFC4 - BGAT2_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Histo-blood group ABO system transferase 2

Gene

Abo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Posseses strong B transferase activity and a weak A transferase activity.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

Cofactori

Binds 1 manganese ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071UDP-N-acetyl-galactosamineBy similarity
Metal bindingi192 – 1921ManganeseBy similarity
Metal bindingi194 – 1941ManganeseBy similarity
Binding sitei214 – 2141Glycoprotein fucosyl-galactosyl groupBy similarity
Binding sitei226 – 2261Glycoprotein fucosyl-galactosyl groupBy similarity
Binding sitei284 – 2841Glycoprotein fucosyl-galactosyl groupBy similarity
Binding sitei307 – 3071Glycoprotein fucosyl-galactosyl groupBy similarity

GO - Molecular functioni

  1. alpha-1,3-galactosyltransferase activity Source: RGD
  2. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: UniProtKB-EC
  3. glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: RGD
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular oligosaccharide metabolic process Source: RGD
  2. negative regulation of apoptotic process Source: RGD
  3. positive regulation of cell proliferation Source: RGD
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. response to nematode Source: RGD
  6. response to retinoic acid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.37. 5301.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Histo-blood group ABO system transferase 2
Alternative name(s):
B blood group galactosyltransferase
Blood group A glycosyltransferase 2
Cis-AB transferase 2
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
Histo-blood group A transferase
Short name:
A transferase
Histo-blood group B transferase
Short name:
B transferase
NAGAT 2
Putative blood group A transferase T2
Gene namesi
Name:Abo2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi2307241. Abo2.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: RGD
  2. extracellular region Source: UniProtKB-KW
  3. Golgi apparatus Source: RGD
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Histo-blood group ABO system transferase 2PRO_0000356180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8CFC4.

Expressioni

Tissue specificityi

Large intestine, caecum, stomach, pancreas, submaxillary gland and kidney (at protein level). Ubiquitous.3 Publications

Gene expression databases

GenevestigatoriQ8CFC4.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039997.

Structurei

3D structure databases

ProteinModelPortaliQ8CFC4.
SMRiQ8CFC4. Positions 49-334.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini37 – 334298LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 1043UDP-N-acetyl-galactosamine bindingBy similarity
Regioni192 – 1943UDP-N-acetyl-galactosamine bindingBy similarity

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similaritiesi

Belongs to the glycosyltransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43612.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8CFC4.
KOiK00709.
OMAiFYKSAEK.
OrthoDBiEOG7BZVSQ.
PhylomeDBiQ8CFC4.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CFC4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDLRFGRLK CYSLHLGILP LTVLVLVFFC FVCLSLRSQE WGHPGAVNRK
60 70 80 90 100
AYPQPRVLTP TRTDVLVLTP WLAPIIWEGT FDIDTLNEQF RLRNTTIGLT
110 120 130 140 150
VFAVKKYVVF LKLFLETAEQ HFMVGHKVIY YVFTDRPADV PQVPLGAGRR
160 170 180 190 200
LVVLTVRNYT RWQDVSMHRM EVISHFSEQR FRHEVDYLVC ADVDMKFRDH
210 220 230 240 250
VGVEILSALF GTLHPGFYRS RRESFTYERR PQSQAYIPWD QGDFYYMGAF
260 270 280 290 300
FGGSVVEVHH LTKACHQAMV EDQANGIEAV WHDESHLNKY LLYHKPTKVL
310 320 330
SPEYMWDQQL LGWPSIMKKL RYVAVPKNHQ AIRN
Length:334
Mass (Da):39,079
Last modified:March 1, 2003 - v1
Checksum:i4EFF83B51393C506
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY228143 mRNA. Translation: AAO72725.1.
AB081652 mRNA. Translation: BAC16248.1.
RefSeqiNP_001153735.1. NM_001160263.1.
UniGeneiRn.112591.

Genome annotation databases

EnsembliENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneIDi100301568.
KEGGirno:100301568.
UCSCiRGD:2307241. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY228143 mRNA. Translation: AAO72725.1 .
AB081652 mRNA. Translation: BAC16248.1 .
RefSeqi NP_001153735.1. NM_001160263.1.
UniGenei Rn.112591.

3D structure databases

ProteinModelPortali Q8CFC4.
SMRi Q8CFC4. Positions 49-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000039997.

Protein family/group databases

CAZyi GT6. Glycosyltransferase Family 6.

Proteomic databases

PRIDEi Q8CFC4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000044775 ; ENSRNOP00000039997 ; ENSRNOG00000046958 .
GeneIDi 100301568.
KEGGi rno:100301568.
UCSCi RGD:2307241. rat.

Organism-specific databases

CTDi 28.
RGDi 2307241. Abo2.

Phylogenomic databases

eggNOGi NOG43612.
GeneTreei ENSGT00400000022032.
HOGENOMi HOG000234339.
HOVERGENi HBG003563.
InParanoidi Q8CFC4.
KOi K00709.
OMAi FYKSAEK.
OrthoDBi EOG7BZVSQ.
PhylomeDBi Q8CFC4.
TreeFami TF330991.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.37. 5301.

Miscellaneous databases

NextBioi 20796023.

Gene expression databases

Genevestigatori Q8CFC4.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10462. PTHR10462. 1 hit.
Pfami PF03414. Glyco_transf_6. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase."
    Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E.
    J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
  2. "Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene."
    Turcot A.-L., Blancher A., Le Moullac-Vaidye B., Despiau S., Rocher J., Roubinet F., Szpirer C., Le Pendu J.
    Glycobiology 13:919-928(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  3. "Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens."
    Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J.
    Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiBGAT2_RAT
AccessioniPrimary (citable) accession number: Q8CFC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3