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Protein

Histo-blood group ABO system transferase 2

Gene

Abo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Posseses strong B transferase activity and a weak A transferase activity.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071UDP-N-acetyl-galactosamineBy similarity
Metal bindingi192 – 1921ManganeseBy similarity
Metal bindingi194 – 1941ManganeseBy similarity
Binding sitei214 – 2141Glycoprotein fucosyl-galactosyl groupBy similarity
Binding sitei226 – 2261Glycoprotein fucosyl-galactosyl groupBy similarity
Binding sitei284 – 2841Glycoprotein fucosyl-galactosyl groupBy similarity
Binding sitei307 – 3071Glycoprotein fucosyl-galactosyl groupBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular oligosaccharide metabolic process Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • protein glycosylation Source: UniProtKB-UniPathway
  • response to nematode Source: RGD
  • response to retinoic acid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.37. 5301.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Histo-blood group ABO system transferase 2
Alternative name(s):
B blood group galactosyltransferase
Blood group A glycosyltransferase 2
Cis-AB transferase 2
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
Histo-blood group A transferase
Short name:
A transferase
Histo-blood group B transferase
Short name:
B transferase
NAGAT 2
Putative blood group A transferase T2
Gene namesi
Name:Abo2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2307241. Abo2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini37 – 334298LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cytoplasmic vesicle Source: RGD
  • extracellular region Source: UniProtKB-SubCell
  • Golgi apparatus Source: RGD
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Histo-blood group ABO system transferase 2PRO_0000356180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8CFC4.

Expressioni

Tissue specificityi

Large intestine, caecum, stomach, pancreas, submaxillary gland and kidney (at protein level). Ubiquitous.3 Publications

Gene expression databases

GenevisibleiQ8CFC4. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039997.

Structurei

3D structure databases

ProteinModelPortaliQ8CFC4.
SMRiQ8CFC4. Positions 49-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 1043UDP-N-acetyl-galactosamine bindingBy similarity
Regioni192 – 1943UDP-N-acetyl-galactosamine bindingBy similarity

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similaritiesi

Belongs to the glycosyltransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43612.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8CFC4.
KOiK00709.
OMAiFCERRFR.
OrthoDBiEOG7BZVSQ.
PhylomeDBiQ8CFC4.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CFC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLRFGRLK CYSLHLGILP LTVLVLVFFC FVCLSLRSQE WGHPGAVNRK
60 70 80 90 100
AYPQPRVLTP TRTDVLVLTP WLAPIIWEGT FDIDTLNEQF RLRNTTIGLT
110 120 130 140 150
VFAVKKYVVF LKLFLETAEQ HFMVGHKVIY YVFTDRPADV PQVPLGAGRR
160 170 180 190 200
LVVLTVRNYT RWQDVSMHRM EVISHFSEQR FRHEVDYLVC ADVDMKFRDH
210 220 230 240 250
VGVEILSALF GTLHPGFYRS RRESFTYERR PQSQAYIPWD QGDFYYMGAF
260 270 280 290 300
FGGSVVEVHH LTKACHQAMV EDQANGIEAV WHDESHLNKY LLYHKPTKVL
310 320 330
SPEYMWDQQL LGWPSIMKKL RYVAVPKNHQ AIRN
Length:334
Mass (Da):39,079
Last modified:March 1, 2003 - v1
Checksum:i4EFF83B51393C506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY228143 mRNA. Translation: AAO72725.1.
AB081652 mRNA. Translation: BAC16248.1.
RefSeqiNP_001153735.1. NM_001160263.1.
UniGeneiRn.112591.

Genome annotation databases

EnsembliENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneIDi100301568.
KEGGirno:100301568.
UCSCiRGD:2307241. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY228143 mRNA. Translation: AAO72725.1.
AB081652 mRNA. Translation: BAC16248.1.
RefSeqiNP_001153735.1. NM_001160263.1.
UniGeneiRn.112591.

3D structure databases

ProteinModelPortaliQ8CFC4.
SMRiQ8CFC4. Positions 49-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039997.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Proteomic databases

PRIDEiQ8CFC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneIDi100301568.
KEGGirno:100301568.
UCSCiRGD:2307241. rat.

Organism-specific databases

CTDi28.
RGDi2307241. Abo2.

Phylogenomic databases

eggNOGiNOG43612.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8CFC4.
KOiK00709.
OMAiFCERRFR.
OrthoDBiEOG7BZVSQ.
PhylomeDBiQ8CFC4.
TreeFamiTF330991.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.37. 5301.

Miscellaneous databases

NextBioi20796023.
PROiQ8CFC4.

Gene expression databases

GenevisibleiQ8CFC4. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase."
    Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E.
    J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
  2. "Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene."
    Turcot A.-L., Blancher A., Le Moullac-Vaidye B., Despiau S., Rocher J., Roubinet F., Szpirer C., Le Pendu J.
    Glycobiology 13:919-928(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  3. "Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens."
    Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J.
    Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiBGAT2_RAT
AccessioniPrimary (citable) accession number: Q8CFC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2003
Last modified: June 24, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.