Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8CFC4

- BGAT2_RAT

UniProt

Q8CFC4 - BGAT2_RAT

Protein

Histo-blood group ABO system transferase 2

Gene

Abo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Posseses strong B transferase activity and a weak A transferase activity.1 Publication

    Catalytic activityi

    UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
    UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071UDP-N-acetyl-galactosamineBy similarity
    Metal bindingi192 – 1921ManganeseBy similarity
    Metal bindingi194 – 1941ManganeseBy similarity
    Binding sitei214 – 2141Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei226 – 2261Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei284 – 2841Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei307 – 3071Glycoprotein fucosyl-galactosyl groupBy similarity

    GO - Molecular functioni

    1. alpha-1,3-galactosyltransferase activity Source: RGD
    2. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: UniProtKB-EC
    3. glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: RGD
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular oligosaccharide metabolic process Source: RGD
    2. negative regulation of apoptotic process Source: RGD
    3. positive regulation of cell proliferation Source: RGD
    4. protein glycosylation Source: UniProtKB-UniPathway
    5. response to nematode Source: RGD
    6. response to retinoic acid Source: RGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BRENDAi2.4.1.37. 5301.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT6. Glycosyltransferase Family 6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histo-blood group ABO system transferase 2
    Alternative name(s):
    B blood group galactosyltransferase
    Blood group A glycosyltransferase 2
    Cis-AB transferase 2
    Fucosylglycoprotein 3-alpha-galactosyltransferase
    Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
    Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
    Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
    Histo-blood group A transferase
    Short name:
    A transferase
    Histo-blood group B transferase
    Short name:
    B transferase
    NAGAT 2
    Putative blood group A transferase T2
    Gene namesi
    Name:Abo2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2307241. Abo2.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
    Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: RGD
    2. extracellular region Source: UniProtKB-SubCell
    3. Golgi apparatus Source: RGD
    4. Golgi cisterna membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 334334Histo-blood group ABO system transferase 2PRO_0000356180Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8CFC4.

    Expressioni

    Tissue specificityi

    Large intestine, caecum, stomach, pancreas, submaxillary gland and kidney (at protein level). Ubiquitous.3 Publications

    Gene expression databases

    GenevestigatoriQ8CFC4.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000039997.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CFC4.
    SMRiQ8CFC4. Positions 49-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini37 – 334298LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni102 – 1043UDP-N-acetyl-galactosamine bindingBy similarity
    Regioni192 – 1943UDP-N-acetyl-galactosamine bindingBy similarity

    Domaini

    The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

    Sequence similaritiesi

    Belongs to the glycosyltransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43612.
    GeneTreeiENSGT00400000022032.
    HOGENOMiHOG000234339.
    HOVERGENiHBG003563.
    KOiK00709.
    OMAiFYKSAEK.
    OrthoDBiEOG7BZVSQ.
    PhylomeDBiQ8CFC4.
    TreeFamiTF330991.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10462. PTHR10462. 1 hit.
    PfamiPF03414. Glyco_transf_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8CFC4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDLRFGRLK CYSLHLGILP LTVLVLVFFC FVCLSLRSQE WGHPGAVNRK    50
    AYPQPRVLTP TRTDVLVLTP WLAPIIWEGT FDIDTLNEQF RLRNTTIGLT 100
    VFAVKKYVVF LKLFLETAEQ HFMVGHKVIY YVFTDRPADV PQVPLGAGRR 150
    LVVLTVRNYT RWQDVSMHRM EVISHFSEQR FRHEVDYLVC ADVDMKFRDH 200
    VGVEILSALF GTLHPGFYRS RRESFTYERR PQSQAYIPWD QGDFYYMGAF 250
    FGGSVVEVHH LTKACHQAMV EDQANGIEAV WHDESHLNKY LLYHKPTKVL 300
    SPEYMWDQQL LGWPSIMKKL RYVAVPKNHQ AIRN 334
    Length:334
    Mass (Da):39,079
    Last modified:March 1, 2003 - v1
    Checksum:i4EFF83B51393C506
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY228143 mRNA. Translation: AAO72725.1.
    AB081652 mRNA. Translation: BAC16248.1.
    RefSeqiNP_001153735.1. NM_001160263.1.
    UniGeneiRn.112591.

    Genome annotation databases

    EnsembliENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
    GeneIDi100301568.
    KEGGirno:100301568.
    UCSCiRGD:2307241. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY228143 mRNA. Translation: AAO72725.1 .
    AB081652 mRNA. Translation: BAC16248.1 .
    RefSeqi NP_001153735.1. NM_001160263.1.
    UniGenei Rn.112591.

    3D structure databases

    ProteinModelPortali Q8CFC4.
    SMRi Q8CFC4. Positions 49-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000039997.

    Protein family/group databases

    CAZyi GT6. Glycosyltransferase Family 6.

    Proteomic databases

    PRIDEi Q8CFC4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000044775 ; ENSRNOP00000039997 ; ENSRNOG00000046958 .
    GeneIDi 100301568.
    KEGGi rno:100301568.
    UCSCi RGD:2307241. rat.

    Organism-specific databases

    CTDi 28.
    RGDi 2307241. Abo2.

    Phylogenomic databases

    eggNOGi NOG43612.
    GeneTreei ENSGT00400000022032.
    HOGENOMi HOG000234339.
    HOVERGENi HBG003563.
    KOi K00709.
    OMAi FYKSAEK.
    OrthoDBi EOG7BZVSQ.
    PhylomeDBi Q8CFC4.
    TreeFami TF330991.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 2.4.1.37. 5301.

    Miscellaneous databases

    NextBioi 20796023.

    Gene expression databases

    Genevestigatori Q8CFC4.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10462. PTHR10462. 1 hit.
    Pfami PF03414. Glyco_transf_6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase."
      Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E.
      J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Wistar.
    2. "Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene."
      Turcot A.-L., Blancher A., Le Moullac-Vaidye B., Despiau S., Rocher J., Roubinet F., Szpirer C., Le Pendu J.
      Glycobiology 13:919-928(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Tissue: Testis.
    3. "Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens."
      Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J.
      Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBGAT2_RAT
    AccessioniPrimary (citable) accession number: Q8CFC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3