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Q8CFC4 (BGAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histo-blood group ABO system transferase 2
Alternative name(s):
B blood group galactosyltransferase
Blood group A glycosyltransferase 2
Cis-AB transferase 2
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
EC=2.4.1.40
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
EC=2.4.1.37
Histo-blood group A transferase
Short name=A transferase
Histo-blood group B transferase
Short name=B transferase
NAGAT 2
Putative blood group A transferase T2
Gene names
Name:Abo2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Posseses strong B transferase activity and a weak A transferase activity. Ref.2

Catalytic activity

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.

UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

Cofactor

Binds 1 manganese ion per subunit By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.

Tissue specificity

Large intestine, caecum, stomach, pancreas, submaxillary gland and kidney (at protein level). Ubiquitous. Ref.1 Ref.2 Ref.3

Domain

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similarities

Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
Secreted
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandManganese
Metal-binding
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular oligosaccharide metabolic process

Inferred from mutant phenotype Ref.2. Source: RGD

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 12499407. Source: RGD

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 12499407. Source: RGD

protein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

response to nematode

Inferred from expression pattern PubMed 11842091. Source: RGD

response to retinoic acid

Inferred from expression pattern PubMed 15042712. Source: RGD

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 17206613. Source: RGD

Golgi cisterna membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle

Inferred from direct assay PubMed 17206613. Source: RGD

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionalpha-1,3-galactosyltransferase activity

Inferred from direct assay PubMed 12626403. Source: RGD

fucosylgalactoside 3-alpha-galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity

Inferred from direct assay PubMed 11842091. Source: RGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Histo-blood group ABO system transferase 2
PRO_0000356180

Regions

Topological domain1 – 1515Cytoplasmic Potential
Transmembrane16 – 3621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain37 – 334298Lumenal Potential
Region102 – 1043UDP-N-acetyl-galactosamine binding By similarity
Region192 – 1943UDP-N-acetyl-galactosamine binding By similarity

Sites

Metal binding1921Manganese By similarity
Metal binding1941Manganese By similarity
Binding site1071UDP-N-acetyl-galactosamine By similarity
Binding site2141Glycoprotein fucosyl-galactosyl group By similarity
Binding site2261Glycoprotein fucosyl-galactosyl group By similarity
Binding site2841Glycoprotein fucosyl-galactosyl group By similarity
Binding site3071Glycoprotein fucosyl-galactosyl group By similarity

Amino acid modifications

Glycosylation941N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8CFC4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 4EFF83B51393C506

FASTA33439,079
        10         20         30         40         50         60 
MKDLRFGRLK CYSLHLGILP LTVLVLVFFC FVCLSLRSQE WGHPGAVNRK AYPQPRVLTP 

        70         80         90        100        110        120 
TRTDVLVLTP WLAPIIWEGT FDIDTLNEQF RLRNTTIGLT VFAVKKYVVF LKLFLETAEQ 

       130        140        150        160        170        180 
HFMVGHKVIY YVFTDRPADV PQVPLGAGRR LVVLTVRNYT RWQDVSMHRM EVISHFSEQR 

       190        200        210        220        230        240 
FRHEVDYLVC ADVDMKFRDH VGVEILSALF GTLHPGFYRS RRESFTYERR PQSQAYIPWD 

       250        260        270        280        290        300 
QGDFYYMGAF FGGSVVEVHH LTKACHQAMV EDQANGIEAV WHDESHLNKY LLYHKPTKVL 

       310        320        330 
SPEYMWDQQL LGWPSIMKKL RYVAVPKNHQ AIRN 

« Hide

References

[1]"Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase."
Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E.
J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Wistar.
[2]"Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene."
Turcot A.-L., Blancher A., Le Moullac-Vaidye B., Despiau S., Rocher J., Roubinet F., Szpirer C., Le Pendu J.
Glycobiology 13:919-928(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Testis.
[3]"Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens."
Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J.
Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY228143 mRNA. Translation: AAO72725.1.
AB081652 mRNA. Translation: BAC16248.1.
RefSeqNP_001153735.1. NM_001160263.1.
UniGeneRn.112591.

3D structure databases

ProteinModelPortalQ8CFC4.
SMRQ8CFC4. Positions 49-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000039997.

Protein family/group databases

CAZyGT6. Glycosyltransferase Family 6.

Proteomic databases

PRIDEQ8CFC4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneID100301568.
KEGGrno:100301568.
UCSCRGD:2307241. rat.

Organism-specific databases

CTD28.
RGD2307241. Abo2.

Phylogenomic databases

eggNOGNOG43612.
GeneTreeENSGT00400000022032.
HOGENOMHOG000234339.
HOVERGENHBG003563.
KOK00709.
OMAELHERDY.
OrthoDBEOG7BZVSQ.
PhylomeDBQ8CFC4.
TreeFamTF330991.

Enzyme and pathway databases

BRENDA2.4.1.37. 5301.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ8CFC4.

Family and domain databases

InterProIPR005076. Glyco_trans_6.
[Graphical view]
PANTHERPTHR10462. PTHR10462. 1 hit.
PfamPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20796023.

Entry information

Entry nameBGAT2_RAT
AccessionPrimary (citable) accession number: Q8CFC4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways