Q8CFC4 (BGAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histo-blood group ABO system transferase 2

Alternative name(s):
B blood group galactosyltransferase
Blood group A glycosyltransferase 2
Cis-AB transferase 2
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
EC=2.4.1.40
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
EC=2.4.1.37
Histo-blood group A transferase
Short name=A transferase
Histo-blood group B transferase
Short name=B transferase
NAGAT 2
Putative blood group A transferase T2

Gene names

Name:

Abo2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Posseses strong B transferase activity and a weak A transferase activity. Ref.2
Catalytic activity	UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose. UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.
Cofactor	Binds 1 manganese ion per subunit By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.
Tissue specificity	Large intestine, caecum, stomach, pancreas, submaxillary gland and kidney (at protein level). Ubiquitous. Ref.1 Ref.2 Ref.3
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular oligosaccharide metabolic process Inferred from mutant phenotype Ref.2. Source: RGD negative regulation of apoptotic process Inferred from mutant phenotype PubMed 12499407. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype PubMed 12499407. Source: RGD protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway response to nematode Inferred from expression pattern PubMed 11842091. Source: RGD response to retinoic acid Inferred from expression pattern PubMed 15042712. Source: RGD
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 17206613. Source: RGD Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from direct assay PubMed 17206613. Source: RGD extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-1,3-galactosyltransferase activity Inferred from direct assay PubMed 12626403. Source: RGD fucosylgalactoside 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Inferred from direct assay PubMed 11842091. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Histo-blood group ABO system transferase 2		PRO_0000356180
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	37 – 334	298	Lumenal Potential
	Region	102 – 104	3	UDP-N-acetyl-galactosamine binding By similarity
	Region	192 – 194	3	UDP-N-acetyl-galactosamine binding By similarity
Sites
	Metal binding	192	1	Manganese By similarity
	Metal binding	194	1	Manganese By similarity
	Binding site	107	1	UDP-N-acetyl-galactosamine By similarity
	Binding site	214	1	Glycoprotein fucosyl-galactosyl group By similarity
	Binding site	226	1	Glycoprotein fucosyl-galactosyl group By similarity
	Binding site	284	1	Glycoprotein fucosyl-galactosyl group By similarity
	Binding site	307	1	Glycoprotein fucosyl-galactosyl group By similarity
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8CFC4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4EFF83B51393C506	FASTA	334	39,079
	10 20 30 40 50 60 MKDLRFGRLK CYSLHLGILP LTVLVLVFFC FVCLSLRSQE WGHPGAVNRK AYPQPRVLTP 70 80 90 100 110 120 TRTDVLVLTP WLAPIIWEGT FDIDTLNEQF RLRNTTIGLT VFAVKKYVVF LKLFLETAEQ 130 140 150 160 170 180 HFMVGHKVIY YVFTDRPADV PQVPLGAGRR LVVLTVRNYT RWQDVSMHRM EVISHFSEQR 190 200 210 220 230 240 FRHEVDYLVC ADVDMKFRDH VGVEILSALF GTLHPGFYRS RRESFTYERR PQSQAYIPWD 250 260 270 280 290 300 QGDFYYMGAF FGGSVVEVHH LTKACHQAMV EDQANGIEAV WHDESHLNKY LLYHKPTKVL 310 320 330 SPEYMWDQQL LGWPSIMKKL RYVAVPKNHQ AIRN

References

[1]	"Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase." Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E. J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Wistar.
[2]	"Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene." Turcot A.-L., Blancher A., Le Moullac-Vaidye B., Despiau S., Rocher J., Roubinet F., Szpirer C., Le Pendu J. Glycobiology 13:919-928(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[3]	"Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens." Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J. Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY228143 mRNA. Translation: AAO72725.1. AB081652 mRNA. Translation: BAC16248.1.
IPI	IPI00480823.
RefSeq	NP_001153735.1. NM_001160263.1.
UniGene	Rn.112591.
3D structure databases
ProteinModelPortal	Q8CFC4.
SMR	Q8CFC4. Positions 49-334.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000039997.
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Proteomic databases
PRIDE	Q8CFC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneID	100301568.
KEGG	rno:100301568.
UCSC	RGD:2307241. rat.
Organism-specific databases
CTD	28.
RGD	2307241. Abo2.
Phylogenomic databases
eggNOG	NOG43612.
GeneTree	ENSGT00400000022032.
HOGENOM	HOG000234339.
HOVERGEN	HBG003563.
KO	K00709.
OrthoDB	EOG4D26Q7.
Enzyme and pathway databases
BRENDA	2.4.1.37. 5301.
UniPathway	UPA00378.
Gene expression databases
Genevestigator	Q8CFC4.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. PTHR10462. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20796023.

Entry information

Entry name

BGAT2_RAT

Accession

Primary (citable) accession number: Q8CFC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program