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Protein

Histo-blood group ABO system transferase 2

Gene

Abo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Posseses strong B transferase activity and a weak A transferase activity.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.By similarity
UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.By similarity

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107UDP-N-acetyl-galactosamineBy similarity1
Metal bindingi192ManganeseBy similarity1
Metal bindingi194ManganeseBy similarity1
Binding sitei214Glycoprotein fucosyl-galactosyl groupBy similarity1
Binding sitei226Glycoprotein fucosyl-galactosyl groupBy similarity1
Active sitei284NucleophileBy similarity1
Binding sitei284Glycoprotein fucosyl-galactosyl groupBy similarity1
Binding sitei307Glycoprotein fucosyl-galactosyl groupBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular oligosaccharide metabolic process Source: RGD
  • glycolipid biosynthetic process Source: GO_Central
  • lipid glycosylation Source: GO_Central
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • protein glycosylation Source: UniProtKB-UniPathway
  • response to nematode Source: RGD
  • response to retinoic acid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.37. 5301.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Histo-blood group ABO system transferase 2
Alternative name(s):
B blood group galactosyltransferase
Blood group A glycosyltransferase 2
Cis-AB transferase 2
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40By similarity)
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37By similarity)
Histo-blood group A transferase
Short name:
A transferase
Histo-blood group B transferase
Short name:
B transferase
NAGAT 2
Putative blood group A transferase T2
Gene namesi
Name:Abo2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2307241. Abo2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini37 – 334LumenalSequence analysisAdd BLAST298

GO - Cellular componenti

  • cytoplasmic vesicle Source: RGD
  • extracellular region Source: UniProtKB-SubCell
  • Golgi apparatus Source: RGD
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003561801 – 334Histo-blood group ABO system transferase 2Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8CFC4.
PRIDEiQ8CFC4.

Expressioni

Tissue specificityi

Large intestine, caecum, stomach, pancreas, submaxillary gland and kidney (at protein level). Ubiquitous.3 Publications

Gene expression databases

BgeeiENSRNOG00000046958.
GenevisibleiQ8CFC4. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039997.

Structurei

3D structure databases

ProteinModelPortaliQ8CFC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 104UDP-N-acetyl-galactosamine bindingBy similarity3
Regioni192 – 194UDP-N-acetyl-galactosamine bindingBy similarity3

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similaritiesi

Belongs to the glycosyltransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFQJ. Eukaryota.
ENOG4111IPQ. LUCA.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8CFC4.
KOiK00709.
OMAiPEYMWDE.
OrthoDBiEOG091G0E0P.
PhylomeDBiQ8CFC4.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CFC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLRFGRLK CYSLHLGILP LTVLVLVFFC FVCLSLRSQE WGHPGAVNRK
60 70 80 90 100
AYPQPRVLTP TRTDVLVLTP WLAPIIWEGT FDIDTLNEQF RLRNTTIGLT
110 120 130 140 150
VFAVKKYVVF LKLFLETAEQ HFMVGHKVIY YVFTDRPADV PQVPLGAGRR
160 170 180 190 200
LVVLTVRNYT RWQDVSMHRM EVISHFSEQR FRHEVDYLVC ADVDMKFRDH
210 220 230 240 250
VGVEILSALF GTLHPGFYRS RRESFTYERR PQSQAYIPWD QGDFYYMGAF
260 270 280 290 300
FGGSVVEVHH LTKACHQAMV EDQANGIEAV WHDESHLNKY LLYHKPTKVL
310 320 330
SPEYMWDQQL LGWPSIMKKL RYVAVPKNHQ AIRN
Length:334
Mass (Da):39,079
Last modified:March 1, 2003 - v1
Checksum:i4EFF83B51393C506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY228143 mRNA. Translation: AAO72725.1.
AB081652 mRNA. Translation: BAC16248.1.
RefSeqiNP_001153735.1. NM_001160263.1.
UniGeneiRn.112591.

Genome annotation databases

EnsembliENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneIDi100301568.
KEGGirno:100301568.
UCSCiRGD:2307241. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY228143 mRNA. Translation: AAO72725.1.
AB081652 mRNA. Translation: BAC16248.1.
RefSeqiNP_001153735.1. NM_001160263.1.
UniGeneiRn.112591.

3D structure databases

ProteinModelPortaliQ8CFC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039997.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Proteomic databases

PaxDbiQ8CFC4.
PRIDEiQ8CFC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000044775; ENSRNOP00000039997; ENSRNOG00000046958.
GeneIDi100301568.
KEGGirno:100301568.
UCSCiRGD:2307241. rat.

Organism-specific databases

CTDi28.
RGDi2307241. Abo2.

Phylogenomic databases

eggNOGiENOG410IFQJ. Eukaryota.
ENOG4111IPQ. LUCA.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8CFC4.
KOiK00709.
OMAiPEYMWDE.
OrthoDBiEOG091G0E0P.
PhylomeDBiQ8CFC4.
TreeFamiTF330991.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.37. 5301.

Miscellaneous databases

PROiQ8CFC4.

Gene expression databases

BgeeiENSRNOG00000046958.
GenevisibleiQ8CFC4. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGAT2_RAT
AccessioniPrimary (citable) accession number: Q8CFC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.