Q8CFC2 (MYT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myelin transcription factor 1
Short name=MyT1

Alternative name(s):
Neural zinc finger factor 2
Short name=NZF-2

Gene names

Name:	Myt1
Synonyms:	Kiaa0835, Nzf2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription By similarity. Ref.1
Subunit structure	Interacts with STEAP3 By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Isoform 1 is more predominant than isoform 2 at all stages of development and adulthood. Expressed in differentiated neurons especially at higher levels in newly generated ones. Ref.2
Developmental stage	Isoform 1 is detected in the earliest born neurons. At 9.5 dpc it is detected in the ventrolateral part of the spinal cord, which later become motor neurons and is also detected in the dispersed cells of the alar plate interneurons. During spinal cord development, the expression is highest in the latest born neurons (the subventricular zone). Detected in the early differentiated neurons within the neuroepithelium and the neural crest cells at 9.5 dpc. At 12.5 dpc, detected in the differentiated neurons within the forebrain, midbrain, and hindbrain. In these neurons, the expression level is highest in the latest born neurons and is also detected in the differentiated neurons of the sensory organs and the peripheral ganglia. Ref.2
Domain	Contains 7 zinc fingers of the C2HC class arranged in two widely separated clusters. These two domains of DNA binding can function independently and recognize the same DNA sequence.
Sequence similarities	Contains 7 C2HC-type zinc fingers.
Sequence caution	The sequence BAC41451.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CFC2-1)

Also known as: NZF-2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CFC2-2)

Also known as: NZF-2a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-152: MSSESDDKRA...SNPTSSPSGF → MMDGIGIRTE...LQPHKQPFWL

Isoform 3 (identifier: Q8CFC2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     508-545: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1127

1127

Myelin transcription factor 1

PRO_0000096677

Regions

Zinc finger

28 – 54

27

C2HC-type 1

Zinc finger

442 – 468

27

C2HC-type 2

Zinc finger

486 – 512

27

C2HC-type 3

Zinc finger

800 – 826

27

C2HC-type 4

Zinc finger

844 – 870

27

C2HC-type 5

Zinc finger

893 – 919

27

C2HC-type 6

Zinc finger

946 – 972

27

C2HC-type 7

Compositional bias

210 – 307

98

Glu-rich

Compositional bias

687 – 725

39

Ser-rich

Natural variations

Alternative sequence

1 – 152

152

MSSES…SPSGF → MMDGIGIRTEKYQSNLAKID SFLVFESRQKADRMAYSSFP YFLSYSAESGQVGIGGELAT VGSRDLETFPHALFKAPLFL VLCRKKPHQAPFLLQPHKQP FWL in isoform 2.

VSP_015720

Alternative sequence

508 – 545

38

Missing in isoform 3.

VSP_015721

Experimental info

Sequence conflict

238

1

P → R in AAC53456. Ref.1

Sequence conflict

446

1

T → A in AAC53456. Ref.1

Sequence conflict

502

1

N → Y in AAC53456. Ref.1

Sequence conflict

701

1

D → G in AAC53456. Ref.1

Sequence conflict

879

1

K → R in AAC53456. Ref.1

Sequence conflict

1022

1

E → G in AAC53456. Ref.1

Sequence conflict

1039

1

K → R in AAC53456. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

1127

Helix Strand Turn

Details...

Beta strand

853 – 855

3

Beta strand

857 – 860

4

Beta strand

865 – 869

5

Turn

871 – 874

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (NZF-2b) [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CB46958837E50008	FASTA	1,127	123,576
	10 20 30 40 50 60 MSSESDDKRA RTRSKTLRGP PETTGADLSC PTPGCTGSGH VRGKYSRHRS LQSCPLAKKR 70 80 90 100 110 120 KLEDAETEHL VSKRKSHPLR LALDEGYRMD SDGSEDAEVK DVSVSDESEG PLEEAEAEMS 130 140 150 160 170 180 GQEEIHHPQT AEGKSLIKPH FDSNPTSSPS GFSKSSYSSY QGIIATSLLN LGQIAEEALV 190 200 210 220 230 240 KEDSVSVAKL SPTVVHQLQD EAAMGVNSDE GEKDLFIQPE DVEEVIEVTS ERSQEPCPQS 250 260 270 280 290 300 LKDMVSEESS KQKGVLGHEE EGEEEEEDEE EEDEEEEEEG EEGEEEEEEE EEEEEEEDEE 310 320 330 340 350 360 EEEEEEEAAP NVIFGEDTSH TSVQKASPEF RGPELSSPKP EYSVIVEVRS DDDKDEDSRS 370 380 390 400 410 420 QKSAVTDESE MYDMMTRGNL GLLEQAIALK AEQVRAVCES GCPPAEQGHL GPGEPGKMAK 430 440 450 460 470 480 PLDVVRKSCY SKDPSRVEKR EIKCPTPGCD GTGHVTGLYP HHRSLSGCPH KDRIPPEILA 490 500 510 520 530 540 MHENVLKCPT PGCTGQGHVN SNRNTHRSLS GCPIAAAEKL AKSHEKQQLQ TGDPPKNNSN 550 560 570 580 590 600 SDRILRPMCF VKQLEVPPYG SYRPNVAPAT PRANLAKELE KFSKVTFDYA SFDAQVFGKR 610 620 630 640 650 660 MLAPKIQTSE TSPKAFQCFD YSHDAEAAHM AATAILNLST RCWEMPENLS TKPQDLPSKA 670 680 690 700 710 720 VDIEVDENGT LDLSMHKHRK RENTFPSSSS CSSSPGVKSP DVSQRQSSTS APSSSMTSPQ 730 740 750 760 770 780 SSQASRQDEW DRPLDYTKPS RLREEEPEES EPAAHSFASS EADDQEVSEE NFEERKYPGE 790 800 810 820 830 840 VTLTNFKLKF LSKDIKKELL TCPTPGCDGS GHITGNYASH RSLSGCPLAD KSLRNLMAAH 850 860 870 880 890 900 SADLKCPTPG CDGSGHITGN YASHRSLSGC PRAKKSGLKV APTKDDKEDP ELMKCPVPGC 910 920 930 940 950 960 VGLGHISGKY ASHRSASGCP LAARRQKEGA LNGSSFSWKS LKNEGPTCPT PGCDGSGHAN 970 980 990 1000 1010 1020 GSFLTHRSLS GCPRATFAGK KGKLSGDEIL SPKFKTSDVL ENDEEIKQLN QEIRDLNESN 1030 1040 1050 1060 1070 1080 SEMEAAMVQL QSQISSMEKN LKNIEEENKL IEEQNEALFL ELSGLSQALI QSLANIRLPH 1090 1100 1110 1120 MEPICEQNFD AYVNTLTDMY SNQDCYQNPE NKGLLETIKQ AVRGIQV
	Isoform 2 (NZF-2a) [UniParc]. Checksum: 6D414F1AE89BE01A	FASTA	1,078	118,701
	10 20 30 40 50 60 MMDGIGIRTE KYQSNLAKID SFLVFESRQK ADRMAYSSFP YFLSYSAESG QVGIGGELAT 70 80 90 100 110 120 VGSRDLETFP HALFKAPLFL VLCRKKPHQA PFLLQPHKQP FWLSKSSYSS YQGIIATSLL 130 140 150 160 170 180 NLGQIAEEAL VKEDSVSVAK LSPTVVHQLQ DEAAMGVNSD EGEKDLFIQP EDVEEVIEVT 190 200 210 220 230 240 SERSQEPCPQ SLKDMVSEES SKQKGVLGHE EEGEEEEEDE EEEDEEEEEE GEEGEEEEEE 250 260 270 280 290 300 EEEEEEEEDE EEEEEEEEAA PNVIFGEDTS HTSVQKASPE FRGPELSSPK PEYSVIVEVR 310 320 330 340 350 360 SDDDKDEDSR SQKSAVTDES EMYDMMTRGN LGLLEQAIAL KAEQVRAVCE SGCPPAEQGH 370 380 390 400 410 420 LGPGEPGKMA KPLDVVRKSC YSKDPSRVEK REIKCPTPGC DGTGHVTGLY PHHRSLSGCP 430 440 450 460 470 480 HKDRIPPEIL AMHENVLKCP TPGCTGQGHV NSNRNTHRSL SGCPIAAAEK LAKSHEKQQL 490 500 510 520 530 540 QTGDPPKNNS NSDRILRPMC FVKQLEVPPY GSYRPNVAPA TPRANLAKEL EKFSKVTFDY 550 560 570 580 590 600 ASFDAQVFGK RMLAPKIQTS ETSPKAFQCF DYSHDAEAAH MAATAILNLS TRCWEMPENL 610 620 630 640 650 660 STKPQDLPSK AVDIEVDENG TLDLSMHKHR KRENTFPSSS SCSSSPGVKS PDVSQRQSST 670 680 690 700 710 720 SAPSSSMTSP QSSQASRQDE WDRPLDYTKP SRLREEEPEE SEPAAHSFAS SEADDQEVSE 730 740 750 760 770 780 ENFEERKYPG EVTLTNFKLK FLSKDIKKEL LTCPTPGCDG SGHITGNYAS HRSLSGCPLA 790 800 810 820 830 840 DKSLRNLMAA HSADLKCPTP GCDGSGHITG NYASHRSLSG CPRAKKSGLK VAPTKDDKED 850 860 870 880 890 900 PELMKCPVPG CVGLGHISGK YASHRSASGC PLAARRQKEG ALNGSSFSWK SLKNEGPTCP 910 920 930 940 950 960 TPGCDGSGHA NGSFLTHRSL SGCPRATFAG KKGKLSGDEI LSPKFKTSDV LENDEEIKQL 970 980 990 1000 1010 1020 NQEIRDLNES NSEMEAAMVQ LQSQISSMEK NLKNIEEENK LIEEQNEALF LELSGLSQAL 1030 1040 1050 1060 1070 IQSLANIRLP HMEPICEQNF DAYVNTLTDM YSNQDCYQNP ENKGLLETIK QAVRGIQV
	Isoform 3 [UniParc]. Checksum: 13E8D20C1F5FFACA	FASTA	1,089	119,547
	10 20 30 40 50 60 MSSESDDKRA RTRSKTLRGP PETTGADLSC PTPGCTGSGH VRGKYSRHRS LQSCPLAKKR 70 80 90 100 110 120 KLEDAETEHL VSKRKSHPLR LALDEGYRMD SDGSEDAEVK DVSVSDESEG PLEEAEAEMS 130 140 150 160 170 180 GQEEIHHPQT AEGKSLIKPH FDSNPTSSPS GFSKSSYSSY QGIIATSLLN LGQIAEEALV 190 200 210 220 230 240 KEDSVSVAKL SPTVVHQLQD EAAMGVNSDE GEKDLFIQPE DVEEVIEVTS ERSQEPCPQS 250 260 270 280 290 300 LKDMVSEESS KQKGVLGHEE EGEEEEEDEE EEDEEEEEEG EEGEEEEEEE EEEEEEEDEE 310 320 330 340 350 360 EEEEEEEAAP NVIFGEDTSH TSVQKASPEF RGPELSSPKP EYSVIVEVRS DDDKDEDSRS 370 380 390 400 410 420 QKSAVTDESE MYDMMTRGNL GLLEQAIALK AEQVRAVCES GCPPAEQGHL GPGEPGKMAK 430 440 450 460 470 480 PLDVVRKSCY SKDPSRVEKR EIKCPTPGCD GTGHVTGLYP HHRSLSGCPH KDRIPPEILA 490 500 510 520 530 540 MHENVLKCPT PGCTGQGHVN SNRNTHRRPM CFVKQLEVPP YGSYRPNVAP ATPRANLAKE 550 560 570 580 590 600 LEKFSKVTFD YASFDAQVFG KRMLAPKIQT SETSPKAFQC FDYSHDAEAA HMAATAILNL 610 620 630 640 650 660 STRCWEMPEN LSTKPQDLPS KAVDIEVDEN GTLDLSMHKH RKRENTFPSS SSCSSSPGVK 670 680 690 700 710 720 SPDVSQRQSS TSAPSSSMTS PQSSQASRQD EWDRPLDYTK PSRLREEEPE ESEPAAHSFA 730 740 750 760 770 780 SSEADDQEVS EENFEERKYP GEVTLTNFKL KFLSKDIKKE LLTCPTPGCD GSGHITGNYA 790 800 810 820 830 840 SHRSLSGCPL ADKSLRNLMA AHSADLKCPT PGCDGSGHIT GNYASHRSLS GCPRAKKSGL 850 860 870 880 890 900 KVAPTKDDKE DPELMKCPVP GCVGLGHISG KYASHRSASG CPLAARRQKE GALNGSSFSW 910 920 930 940 950 960 KSLKNEGPTC PTPGCDGSGH ANGSFLTHRS LSGCPRATFA GKKGKLSGDE ILSPKFKTSD 970 980 990 1000 1010 1020 VLENDEEIKQ LNQEIRDLNE SNSEMEAAMV QLQSQISSME KNLKNIEEEN KLIEEQNEAL 1030 1040 1050 1060 1070 1080 FLELSGLSQA LIQSLANIRL PHMEPICEQN FDAYVNTLTD MYSNQDCYQN PENKGLLETI KQAVRGIQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system." Kim J.G., Armstrong R.C., Agoston D.V., Robinsky A., Wiese C., Nagle J., Hudson L.D. J. Neurosci. Res. 50:272-290(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
[2]	"NZF-2b is a novel predominant form of mouse NZF-2/MyT1, expressed in differentiated neurons especially at higher levels in newly generated ones." Matsushita F., Kameyama T., Marunouchi T. Mech. Dev. 118:209-213(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: ICR. Tissue: Brain.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H. DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Fetal brain.
[4]	Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[5]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[7]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 537-543, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[8]	"Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1." Gamsjaeger R., Swanton M.K., Kobus F.J., Lehtomaki E., Lowry J.A., Kwan A.H., Matthews J.M., Mackay J.P. J. Biol. Chem. 283:5158-5167(2008) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 837-878, DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF004294 mRNA. Translation: AAC53456.1.
AB082378 mRNA. Translation: BAC16512.1.
AB093267 mRNA. Translation: BAC41451.2. Different initiation.
AL845173 Genomic DNA. Translation: CAM20798.1.
AL845173 Genomic DNA. Translation: CAM20799.1.
BC063252 mRNA. Translation: AAH63252.1.

IPI

IPI00229281.
IPI00265444.
IPI00654203.

PIR

T42712.

RefSeq

NP_001165086.1. NM_001171615.1.
NP_001165087.1. NM_001171616.1.
NP_032691.2. NM_008665.4.

UniGene

Mm.458718.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JX1	NMR	-	A	843-873	[»]
2JYD	NMR	-	A	837-878	[»]

ProteinModelPortal

Q8CFC2.

SMR

Q8CFC2. Positions 26-62, 440-516, 800-985.

ModBase

Search...

PTM databases

PhosphoSite

Q8CFC2.

Proteomic databases

PRIDE

Q8CFC2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000108756; ENSMUSP00000104387; ENSMUSG00000010505.
ENSMUST00000108757; ENSMUSP00000104388; ENSMUSG00000010505.

GeneID

17932.

KEGG

mmu:17932.

UCSC

uc012cmw.1. mouse.
uc012cmx.1. mouse.

Organism-specific databases

CTD

4661.

MGI

MGI:1100535. Myt1.

Rouge

Search...

Phylogenomic databases

eggNOG

NOG329034.

GeneTree

ENSGT00440000038465.

HOGENOM

HOG000234099.

HOVERGEN

HBG006433.

InParanoid

B0R0C3.

OrthoDB

EOG4RBQJ2.

Gene expression databases

ArrayExpress

Q8CFC2.

Bgee

Q8CFC2.

CleanEx

MM_MYT1.

Genevestigator

Q8CFC2.

GermOnline

ENSMUSG00000010505. Mus musculus.

Family and domain databases

InterPro

IPR013681. Myelin_TF.
IPR002515. Znf_C2HC.
[Graphical view]

Pfam

PF08474. MYT1. 2 hits.
PF01530. zf-C2HC. 7 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8CFC2.

NextBio

292819.

SOURCE

Search...

Entry information

Entry name

MYT1_MOUSE

Accession

Primary (citable) accession number: Q8CFC2
Secondary accession number(s): B0R0C3 , B0R0C4, O08995, Q8CFH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program