Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8CFC2

- MYT1_MOUSE

UniProt

Q8CFC2 - MYT1_MOUSE

Protein

Myelin transcription factor 1

Gene

Myt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 5427C2HC-type 1Add
    BLAST
    Zinc fingeri442 – 46827C2HC-type 2Add
    BLAST
    Zinc fingeri486 – 51227C2HC-type 3Add
    BLAST
    Zinc fingeri800 – 82627C2HC-type 4Add
    BLAST
    Zinc fingeri844 – 87027C2HC-type 5Add
    BLAST
    Zinc fingeri893 – 91927C2HC-type 6Add
    BLAST
    Zinc fingeri946 – 97227C2HC-type 7Add
    BLAST

    GO - Molecular functioni

    1. enhancer sequence-specific DNA binding Source: MGI
    2. protein binding Source: MGI
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. nervous system development Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myelin transcription factor 1
    Short name:
    MyT1
    Alternative name(s):
    Neural zinc finger factor 2
    Short name:
    NZF-2
    Gene namesi
    Name:Myt1
    Synonyms:Kiaa0835, Nzf2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1100535. Myt1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11271127Myelin transcription factor 1PRO_0000096677Add
    BLAST

    Proteomic databases

    PRIDEiQ8CFC2.

    PTM databases

    PhosphoSiteiQ8CFC2.

    Expressioni

    Tissue specificityi

    Isoform 1 is more predominant than isoform 2 at all stages of development and adulthood. Expressed in differentiated neurons especially at higher levels in newly generated ones.1 Publication

    Developmental stagei

    Isoform 1 is detected in the earliest born neurons. At 9.5 dpc it is detected in the ventrolateral part of the spinal cord, which later become motor neurons and is also detected in the dispersed cells of the alar plate interneurons. During spinal cord development, the expression is highest in the latest born neurons (the subventricular zone). Detected in the early differentiated neurons within the neuroepithelium and the neural crest cells at 9.5 dpc. At 12.5 dpc, detected in the differentiated neurons within the forebrain, midbrain, and hindbrain. In these neurons, the expression level is highest in the latest born neurons and is also detected in the differentiated neurons of the sensory organs and the peripheral ganglia.1 Publication

    Gene expression databases

    BgeeiQ8CFC2.
    CleanExiMM_MYT1.
    GenevestigatoriQ8CFC2.

    Interactioni

    Subunit structurei

    Interacts with STEAP3.By similarity

    Protein-protein interaction databases

    BioGridi201678. 1 interaction.

    Structurei

    Secondary structure

    1
    1127
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi801 – 8033
    Beta strandi813 – 8164
    Beta strandi821 – 8244
    Beta strandi853 – 8553
    Beta strandi857 – 8604
    Beta strandi865 – 8695
    Turni871 – 8744

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JX1NMR-A843-873[»]
    2JYDNMR-A837-878[»]
    2MF8NMR-A792-878[»]
    ProteinModelPortaliQ8CFC2.
    SMRiQ8CFC2. Positions 28-59, 440-515, 799-985.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8CFC2.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi210 – 30798Glu-richAdd
    BLAST
    Compositional biasi687 – 72539Ser-richAdd
    BLAST

    Domaini

    Sequence similaritiesi

    Contains 7 C2HC-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 5427C2HC-type 1Add
    BLAST
    Zinc fingeri442 – 46827C2HC-type 2Add
    BLAST
    Zinc fingeri486 – 51227C2HC-type 3Add
    BLAST
    Zinc fingeri800 – 82627C2HC-type 4Add
    BLAST
    Zinc fingeri844 – 87027C2HC-type 5Add
    BLAST
    Zinc fingeri893 – 91927C2HC-type 6Add
    BLAST
    Zinc fingeri946 – 97227C2HC-type 7Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG329034.
    GeneTreeiENSGT00440000038465.
    HOGENOMiHOG000234099.
    HOVERGENiHBG006433.
    InParanoidiB0R0C3.

    Family and domain databases

    InterProiIPR013681. Myelin_TF.
    IPR002515. Znf_C2HC.
    [Graphical view]
    PfamiPF08474. MYT1. 2 hits.
    PF01530. zf-C2HC. 7 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8CFC2-1) [UniParc]FASTAAdd to Basket

    Also known as: NZF-2b

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSESDDKRA RTRSKTLRGP PETTGADLSC PTPGCTGSGH VRGKYSRHRS     50
    LQSCPLAKKR KLEDAETEHL VSKRKSHPLR LALDEGYRMD SDGSEDAEVK 100
    DVSVSDESEG PLEEAEAEMS GQEEIHHPQT AEGKSLIKPH FDSNPTSSPS 150
    GFSKSSYSSY QGIIATSLLN LGQIAEEALV KEDSVSVAKL SPTVVHQLQD 200
    EAAMGVNSDE GEKDLFIQPE DVEEVIEVTS ERSQEPCPQS LKDMVSEESS 250
    KQKGVLGHEE EGEEEEEDEE EEDEEEEEEG EEGEEEEEEE EEEEEEEDEE 300
    EEEEEEEAAP NVIFGEDTSH TSVQKASPEF RGPELSSPKP EYSVIVEVRS 350
    DDDKDEDSRS QKSAVTDESE MYDMMTRGNL GLLEQAIALK AEQVRAVCES 400
    GCPPAEQGHL GPGEPGKMAK PLDVVRKSCY SKDPSRVEKR EIKCPTPGCD 450
    GTGHVTGLYP HHRSLSGCPH KDRIPPEILA MHENVLKCPT PGCTGQGHVN 500
    SNRNTHRSLS GCPIAAAEKL AKSHEKQQLQ TGDPPKNNSN SDRILRPMCF 550
    VKQLEVPPYG SYRPNVAPAT PRANLAKELE KFSKVTFDYA SFDAQVFGKR 600
    MLAPKIQTSE TSPKAFQCFD YSHDAEAAHM AATAILNLST RCWEMPENLS 650
    TKPQDLPSKA VDIEVDENGT LDLSMHKHRK RENTFPSSSS CSSSPGVKSP 700
    DVSQRQSSTS APSSSMTSPQ SSQASRQDEW DRPLDYTKPS RLREEEPEES 750
    EPAAHSFASS EADDQEVSEE NFEERKYPGE VTLTNFKLKF LSKDIKKELL 800
    TCPTPGCDGS GHITGNYASH RSLSGCPLAD KSLRNLMAAH SADLKCPTPG 850
    CDGSGHITGN YASHRSLSGC PRAKKSGLKV APTKDDKEDP ELMKCPVPGC 900
    VGLGHISGKY ASHRSASGCP LAARRQKEGA LNGSSFSWKS LKNEGPTCPT 950
    PGCDGSGHAN GSFLTHRSLS GCPRATFAGK KGKLSGDEIL SPKFKTSDVL 1000
    ENDEEIKQLN QEIRDLNESN SEMEAAMVQL QSQISSMEKN LKNIEEENKL 1050
    IEEQNEALFL ELSGLSQALI QSLANIRLPH MEPICEQNFD AYVNTLTDMY 1100
    SNQDCYQNPE NKGLLETIKQ AVRGIQV 1127
    Length:1,127
    Mass (Da):123,576
    Last modified:March 1, 2003 - v1
    Checksum:iCB46958837E50008
    GO
    Isoform 2 (identifier: Q8CFC2-2) [UniParc]FASTAAdd to Basket

    Also known as: NZF-2a

    The sequence of this isoform differs from the canonical sequence as follows:
         1-152: MSSESDDKRA...SNPTSSPSGF → MMDGIGIRTE...LQPHKQPFWL

    Show »
    Length:1,078
    Mass (Da):118,701
    Checksum:i6D414F1AE89BE01A
    GO
    Isoform 3 (identifier: Q8CFC2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         508-545: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,089
    Mass (Da):119,547
    Checksum:i13E8D20C1F5FFACA
    GO

    Sequence cautioni

    The sequence BAC41451.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti238 – 2381P → R in AAC53456. (PubMed:9373037)Curated
    Sequence conflicti446 – 4461T → A in AAC53456. (PubMed:9373037)Curated
    Sequence conflicti502 – 5021N → Y in AAC53456. (PubMed:9373037)Curated
    Sequence conflicti701 – 7011D → G in AAC53456. (PubMed:9373037)Curated
    Sequence conflicti879 – 8791K → R in AAC53456. (PubMed:9373037)Curated
    Sequence conflicti1022 – 10221E → G in AAC53456. (PubMed:9373037)Curated
    Sequence conflicti1039 – 10391K → R in AAC53456. (PubMed:9373037)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 152152MSSES…SPSGF → MMDGIGIRTEKYQSNLAKID SFLVFESRQKADRMAYSSFP YFLSYSAESGQVGIGGELAT VGSRDLETFPHALFKAPLFL VLCRKKPHQAPFLLQPHKQP FWL in isoform 2. 1 PublicationVSP_015720Add
    BLAST
    Alternative sequencei508 – 54538Missing in isoform 3. 1 PublicationVSP_015721Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004294 mRNA. Translation: AAC53456.1.
    AB082378 mRNA. Translation: BAC16512.1.
    AB093267 mRNA. Translation: BAC41451.2. Different initiation.
    AL845173 Genomic DNA. Translation: CAM20798.1.
    AL845173 Genomic DNA. Translation: CAM20799.1.
    BC063252 mRNA. Translation: AAH63252.1.
    CCDSiCCDS17223.1. [Q8CFC2-1]
    CCDS50857.1. [Q8CFC2-3]
    PIRiT42712.
    RefSeqiNP_001165086.1. NM_001171615.1.
    NP_001165087.1. NM_001171616.1. [Q8CFC2-3]
    NP_032691.2. NM_008665.4. [Q8CFC2-1]
    UniGeneiMm.458718.

    Genome annotation databases

    EnsembliENSMUST00000108756; ENSMUSP00000104387; ENSMUSG00000010505. [Q8CFC2-1]
    ENSMUST00000108757; ENSMUSP00000104388; ENSMUSG00000010505. [Q8CFC2-3]
    GeneIDi17932.
    KEGGimmu:17932.
    UCSCiuc008ons.2. mouse. [Q8CFC2-1]
    uc012cmw.1. mouse. [Q8CFC2-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004294 mRNA. Translation: AAC53456.1 .
    AB082378 mRNA. Translation: BAC16512.1 .
    AB093267 mRNA. Translation: BAC41451.2 . Different initiation.
    AL845173 Genomic DNA. Translation: CAM20798.1 .
    AL845173 Genomic DNA. Translation: CAM20799.1 .
    BC063252 mRNA. Translation: AAH63252.1 .
    CCDSi CCDS17223.1. [Q8CFC2-1 ]
    CCDS50857.1. [Q8CFC2-3 ]
    PIRi T42712.
    RefSeqi NP_001165086.1. NM_001171615.1.
    NP_001165087.1. NM_001171616.1. [Q8CFC2-3 ]
    NP_032691.2. NM_008665.4. [Q8CFC2-1 ]
    UniGenei Mm.458718.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JX1 NMR - A 843-873 [» ]
    2JYD NMR - A 837-878 [» ]
    2MF8 NMR - A 792-878 [» ]
    ProteinModelPortali Q8CFC2.
    SMRi Q8CFC2. Positions 28-59, 440-515, 799-985.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201678. 1 interaction.

    PTM databases

    PhosphoSitei Q8CFC2.

    Proteomic databases

    PRIDEi Q8CFC2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108756 ; ENSMUSP00000104387 ; ENSMUSG00000010505 . [Q8CFC2-1 ]
    ENSMUST00000108757 ; ENSMUSP00000104388 ; ENSMUSG00000010505 . [Q8CFC2-3 ]
    GeneIDi 17932.
    KEGGi mmu:17932.
    UCSCi uc008ons.2. mouse. [Q8CFC2-1 ]
    uc012cmw.1. mouse. [Q8CFC2-3 ]

    Organism-specific databases

    CTDi 4661.
    MGIi MGI:1100535. Myt1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG329034.
    GeneTreei ENSGT00440000038465.
    HOGENOMi HOG000234099.
    HOVERGENi HBG006433.
    InParanoidi B0R0C3.

    Miscellaneous databases

    EvolutionaryTracei Q8CFC2.
    NextBioi 292819.
    PROi Q8CFC2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CFC2.
    CleanExi MM_MYT1.
    Genevestigatori Q8CFC2.

    Family and domain databases

    InterProi IPR013681. Myelin_TF.
    IPR002515. Znf_C2HC.
    [Graphical view ]
    Pfami PF08474. MYT1. 2 hits.
    PF01530. zf-C2HC. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system."
      Kim J.G., Armstrong R.C., Agoston D.V., Robinsky A., Wiese C., Nagle J., Hudson L.D.
      J. Neurosci. Res. 50:272-290(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
    2. "NZF-2b is a novel predominant form of mouse NZF-2/MyT1, expressed in differentiated neurons especially at higher levels in newly generated ones."
      Matsushita F., Kameyama T., Marunouchi T.
      Mech. Dev. 118:209-213(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: ICR.
      Tissue: Brain.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
      DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Fetal brain.
    4. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    7. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 537-543, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    8. "Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1."
      Gamsjaeger R., Swanton M.K., Kobus F.J., Lehtomaki E., Lowry J.A., Kwan A.H., Matthews J.M., Mackay J.P.
      J. Biol. Chem. 283:5158-5167(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 837-878, DNA-BINDING.

    Entry informationi

    Entry nameiMYT1_MOUSE
    AccessioniPrimary (citable) accession number: Q8CFC2
    Secondary accession number(s): B0R0C3
    , B0R0C4, O08995, Q8CFH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3