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Q8CFC2 (MYT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myelin transcription factor 1

Short name=MyT1
Alternative name(s):
Neural zinc finger factor 2
Short name=NZF-2
Gene names
Name:Myt1
Synonyms:Kiaa0835, Nzf2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1127 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription By similarity. Ref.1

Subunit structure

Interacts with STEAP3 By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Isoform 1 is more predominant than isoform 2 at all stages of development and adulthood. Expressed in differentiated neurons especially at higher levels in newly generated ones. Ref.2

Developmental stage

Isoform 1 is detected in the earliest born neurons. At 9.5 dpc it is detected in the ventrolateral part of the spinal cord, which later become motor neurons and is also detected in the dispersed cells of the alar plate interneurons. During spinal cord development, the expression is highest in the latest born neurons (the subventricular zone). Detected in the early differentiated neurons within the neuroepithelium and the neural crest cells at 9.5 dpc. At 12.5 dpc, detected in the differentiated neurons within the forebrain, midbrain, and hindbrain. In these neurons, the expression level is highest in the latest born neurons and is also detected in the differentiated neurons of the sensory organs and the peripheral ganglia. Ref.2

Domain

Contains 7 zinc fingers of the C2HC class arranged in two widely separated clusters. These two domains of DNA binding can function independently and recognize the same DNA sequence.

Sequence similarities

Contains 7 C2HC-type zinc fingers.

Sequence caution

The sequence BAC41451.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CFC2-1)

Also known as: NZF-2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CFC2-2)

Also known as: NZF-2a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-152: MSSESDDKRA...SNPTSSPSGF → MMDGIGIRTE...LQPHKQPFWL
Isoform 3 (identifier: Q8CFC2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     508-545: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11271127Myelin transcription factor 1
PRO_0000096677

Regions

Zinc finger28 – 5427C2HC-type 1
Zinc finger442 – 46827C2HC-type 2
Zinc finger486 – 51227C2HC-type 3
Zinc finger800 – 82627C2HC-type 4
Zinc finger844 – 87027C2HC-type 5
Zinc finger893 – 91927C2HC-type 6
Zinc finger946 – 97227C2HC-type 7
Compositional bias210 – 30798Glu-rich
Compositional bias687 – 72539Ser-rich

Natural variations

Alternative sequence1 – 152152MSSES…SPSGF → MMDGIGIRTEKYQSNLAKID SFLVFESRQKADRMAYSSFP YFLSYSAESGQVGIGGELAT VGSRDLETFPHALFKAPLFL VLCRKKPHQAPFLLQPHKQP FWL in isoform 2.
VSP_015720
Alternative sequence508 – 54538Missing in isoform 3.
VSP_015721

Experimental info

Sequence conflict2381P → R in AAC53456. Ref.1
Sequence conflict4461T → A in AAC53456. Ref.1
Sequence conflict5021N → Y in AAC53456. Ref.1
Sequence conflict7011D → G in AAC53456. Ref.1
Sequence conflict8791K → R in AAC53456. Ref.1
Sequence conflict10221E → G in AAC53456. Ref.1
Sequence conflict10391K → R in AAC53456. Ref.1

Secondary structure

............... 1127
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (NZF-2b) [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: CB46958837E50008

FASTA1,127123,576
        10         20         30         40         50         60 
MSSESDDKRA RTRSKTLRGP PETTGADLSC PTPGCTGSGH VRGKYSRHRS LQSCPLAKKR 

        70         80         90        100        110        120 
KLEDAETEHL VSKRKSHPLR LALDEGYRMD SDGSEDAEVK DVSVSDESEG PLEEAEAEMS 

       130        140        150        160        170        180 
GQEEIHHPQT AEGKSLIKPH FDSNPTSSPS GFSKSSYSSY QGIIATSLLN LGQIAEEALV 

       190        200        210        220        230        240 
KEDSVSVAKL SPTVVHQLQD EAAMGVNSDE GEKDLFIQPE DVEEVIEVTS ERSQEPCPQS 

       250        260        270        280        290        300 
LKDMVSEESS KQKGVLGHEE EGEEEEEDEE EEDEEEEEEG EEGEEEEEEE EEEEEEEDEE 

       310        320        330        340        350        360 
EEEEEEEAAP NVIFGEDTSH TSVQKASPEF RGPELSSPKP EYSVIVEVRS DDDKDEDSRS 

       370        380        390        400        410        420 
QKSAVTDESE MYDMMTRGNL GLLEQAIALK AEQVRAVCES GCPPAEQGHL GPGEPGKMAK 

       430        440        450        460        470        480 
PLDVVRKSCY SKDPSRVEKR EIKCPTPGCD GTGHVTGLYP HHRSLSGCPH KDRIPPEILA 

       490        500        510        520        530        540 
MHENVLKCPT PGCTGQGHVN SNRNTHRSLS GCPIAAAEKL AKSHEKQQLQ TGDPPKNNSN 

       550        560        570        580        590        600 
SDRILRPMCF VKQLEVPPYG SYRPNVAPAT PRANLAKELE KFSKVTFDYA SFDAQVFGKR 

       610        620        630        640        650        660 
MLAPKIQTSE TSPKAFQCFD YSHDAEAAHM AATAILNLST RCWEMPENLS TKPQDLPSKA 

       670        680        690        700        710        720 
VDIEVDENGT LDLSMHKHRK RENTFPSSSS CSSSPGVKSP DVSQRQSSTS APSSSMTSPQ 

       730        740        750        760        770        780 
SSQASRQDEW DRPLDYTKPS RLREEEPEES EPAAHSFASS EADDQEVSEE NFEERKYPGE 

       790        800        810        820        830        840 
VTLTNFKLKF LSKDIKKELL TCPTPGCDGS GHITGNYASH RSLSGCPLAD KSLRNLMAAH 

       850        860        870        880        890        900 
SADLKCPTPG CDGSGHITGN YASHRSLSGC PRAKKSGLKV APTKDDKEDP ELMKCPVPGC 

       910        920        930        940        950        960 
VGLGHISGKY ASHRSASGCP LAARRQKEGA LNGSSFSWKS LKNEGPTCPT PGCDGSGHAN 

       970        980        990       1000       1010       1020 
GSFLTHRSLS GCPRATFAGK KGKLSGDEIL SPKFKTSDVL ENDEEIKQLN QEIRDLNESN 

      1030       1040       1050       1060       1070       1080 
SEMEAAMVQL QSQISSMEKN LKNIEEENKL IEEQNEALFL ELSGLSQALI QSLANIRLPH 

      1090       1100       1110       1120 
MEPICEQNFD AYVNTLTDMY SNQDCYQNPE NKGLLETIKQ AVRGIQV 

« Hide

Isoform 2 (NZF-2a) [UniParc].

Checksum: 6D414F1AE89BE01A
Show »

FASTA1,078118,701
Isoform 3 [UniParc].

Checksum: 13E8D20C1F5FFACA
Show »

FASTA1,089119,547

References

« Hide 'large scale' references
[1]"Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system."
Kim J.G., Armstrong R.C., Agoston D.V., Robinsky A., Wiese C., Nagle J., Hudson L.D.
J. Neurosci. Res. 50:272-290(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
[2]"NZF-2b is a novel predominant form of mouse NZF-2/MyT1, expressed in differentiated neurons especially at higher levels in newly generated ones."
Matsushita F., Kameyama T., Marunouchi T.
Mech. Dev. 118:209-213(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: ICR.
Tissue: Brain.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Fetal brain.
[4]Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[7]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 537-543, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[8]"Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1."
Gamsjaeger R., Swanton M.K., Kobus F.J., Lehtomaki E., Lowry J.A., Kwan A.H., Matthews J.M., Mackay J.P.
J. Biol. Chem. 283:5158-5167(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 837-878, DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF004294 mRNA. Translation: AAC53456.1.
AB082378 mRNA. Translation: BAC16512.1.
AB093267 mRNA. Translation: BAC41451.2. Different initiation.
AL845173 Genomic DNA. Translation: CAM20798.1.
AL845173 Genomic DNA. Translation: CAM20799.1.
BC063252 mRNA. Translation: AAH63252.1.
CCDSCCDS17223.1. [Q8CFC2-1]
CCDS50857.1. [Q8CFC2-3]
PIRT42712.
RefSeqNP_001165086.1. NM_001171615.1.
NP_001165087.1. NM_001171616.1. [Q8CFC2-3]
NP_032691.2. NM_008665.4. [Q8CFC2-1]
UniGeneMm.458718.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JX1NMR-A843-873[»]
2JYDNMR-A837-878[»]
2MF8NMR-A792-878[»]
ProteinModelPortalQ8CFC2.
SMRQ8CFC2. Positions 28-59, 440-515, 799-985.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201678. 1 interaction.

PTM databases

PhosphoSiteQ8CFC2.

Proteomic databases

PRIDEQ8CFC2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000108756; ENSMUSP00000104387; ENSMUSG00000010505. [Q8CFC2-1]
ENSMUST00000108757; ENSMUSP00000104388; ENSMUSG00000010505. [Q8CFC2-3]
GeneID17932.
KEGGmmu:17932.
UCSCuc008ons.2. mouse. [Q8CFC2-1]
uc012cmw.1. mouse. [Q8CFC2-3]

Organism-specific databases

CTD4661.
MGIMGI:1100535. Myt1.
RougeSearch...

Phylogenomic databases

eggNOGNOG329034.
GeneTreeENSGT00440000038465.
HOGENOMHOG000234099.
HOVERGENHBG006433.
InParanoidB0R0C3.

Gene expression databases

BgeeQ8CFC2.
CleanExMM_MYT1.
GenevestigatorQ8CFC2.

Family and domain databases

InterProIPR013681. Myelin_TF.
IPR002515. Znf_C2HC.
[Graphical view]
PfamPF08474. MYT1. 2 hits.
PF01530. zf-C2HC. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8CFC2.
NextBio292819.
PROQ8CFC2.
SOURCESearch...

Entry information

Entry nameMYT1_MOUSE
AccessionPrimary (citable) accession number: Q8CFC2
Secondary accession number(s): B0R0C3 expand/collapse secondary AC list , B0R0C4, O08995, Q8CFH1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot