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Protein

Aminomethyltransferase, mitochondrial

Gene

Amt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine.By similarity

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei232 – 2321SubstrateBy similarity
Binding sitei261 – 2611SubstrateBy similarity
Binding sitei399 – 3991SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminomethyltransferase, mitochondrialCurated (EC:2.1.2.10By similarity)
Alternative name(s):
Glycine cleavage system T proteinBy similarity
Short name:
GCVT
Gene namesi
Name:AmtImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:3646700. Amt.

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionBy similarityAdd
BLAST
Chaini29 – 403375Aminomethyltransferase, mitochondrialPRO_0000010756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei368 – 3681N6-succinyllysineCombined sources

Proteomic databases

MaxQBiQ8CFA2.
PaxDbiQ8CFA2.
PRIDEiQ8CFA2.

PTM databases

iPTMnetiQ8CFA2.
PhosphoSiteiQ8CFA2.

Expressioni

Gene expression databases

BgeeiQ8CFA2.
CleanExiMM_AMT.
ExpressionAtlasiQ8CFA2. baseline and differential.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.By similarity

Protein-protein interaction databases

IntActiQ8CFA2. 2 interactions.
MINTiMINT-1846242.
STRINGi10090.ENSMUSP00000035230.

Structurei

3D structure databases

ProteinModelPortaliQ8CFA2.
SMRiQ8CFA2. Positions 32-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2770. Eukaryota.
COG0404. LUCA.
GeneTreeiENSGT00530000063120.
HOGENOMiHOG000239380.
HOVERGENiHBG005822.
InParanoidiQ8CFA2.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG7KM5SQ.
PhylomeDBiQ8CFA2.
TreeFamiTF313026.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CFA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRIVSVVAP LGFRLQAQPL VQSRPLSSVQ DVLRRTPLYD FHLAHGGKMV
60 70 80 90 100
AFAGWSLPVQ YRDSHVDSHL HTRRHCSLFD VSHMLQTKIF GCDRVKLLES
110 120 130 140 150
VVVGDIAELR PNQGTLSLFT NEAGGILDDL IVSNTSEGHL YVVSNAGCRD
160 170 180 190 200
KDLALMQDKV KEFQNRGLDV GLEVVENALL ALQGPTATQV LQAGVTDDMK
210 220 230 240 250
KLPFMTSAVM EVFGVSGCRV TRCGYTGEDG VEISVPATGA VHLATTLLKN
260 270 280 290 300
PEVKLAGLAA RDSLRLEAGL CLYGNDIDEH TTPVEGSLSW TLGKRRRIAM
310 320 330 340 350
DFPGAKIIVP QLKGEVQRRR VGLICEGAPV RAHSPILNTE GTVIGTVTSG
360 370 380 390 400
CPSPSLKKNV AMGYVPFKYS RPGTQLLVEV RRKQQMTVVS KMPFVPTNYY

TLK
Length:403
Mass (Da):44,009
Last modified:March 1, 2003 - v1
Checksum:i4FDEC0DC81492167
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ437692 Genomic DNA. Translation: CAD26917.1.
CCDSiCCDS23519.1.
RefSeqiNP_001013836.1. NM_001013814.1.
UniGeneiMm.390225.

Genome annotation databases

EnsembliENSMUST00000035230; ENSMUSP00000035230; ENSMUSG00000032607.
GeneIDi434437.
KEGGimmu:434437.
UCSCiuc009rpc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ437692 Genomic DNA. Translation: CAD26917.1.
CCDSiCCDS23519.1.
RefSeqiNP_001013836.1. NM_001013814.1.
UniGeneiMm.390225.

3D structure databases

ProteinModelPortaliQ8CFA2.
SMRiQ8CFA2. Positions 32-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CFA2. 2 interactions.
MINTiMINT-1846242.
STRINGi10090.ENSMUSP00000035230.

PTM databases

iPTMnetiQ8CFA2.
PhosphoSiteiQ8CFA2.

Proteomic databases

MaxQBiQ8CFA2.
PaxDbiQ8CFA2.
PRIDEiQ8CFA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035230; ENSMUSP00000035230; ENSMUSG00000032607.
GeneIDi434437.
KEGGimmu:434437.
UCSCiuc009rpc.1. mouse.

Organism-specific databases

CTDi275.
MGIiMGI:3646700. Amt.

Phylogenomic databases

eggNOGiKOG2770. Eukaryota.
COG0404. LUCA.
GeneTreeiENSGT00530000063120.
HOGENOMiHOG000239380.
HOVERGENiHBG005822.
InParanoidiQ8CFA2.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG7KM5SQ.
PhylomeDBiQ8CFA2.
TreeFamiTF313026.

Enzyme and pathway databases

ReactomeiR-MMU-6783984. Glycine degradation.

Miscellaneous databases

PROiQ8CFA2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CFA2.
CleanExiMM_AMT.
ExpressionAtlasiQ8CFA2. baseline and differential.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of the murine aminomethyltransferase gene (Amt)."
    Backofen B., Leeb T.
    DNA Seq. 13:179-183(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129S6/SvEvTac.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver and Pancreas.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGCST_MOUSE
AccessioniPrimary (citable) accession number: Q8CFA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.