Interleukin-1 receptor-associated kinase-like 2

Preferred order of sections

Names and origin

Protein names

Recommended name:
Interleukin-1 receptor-associated kinase-like 2
Short name=IRAK-2
Short name=mu-IRAK-2

Gene names

Name:

Irak2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization By similarity.
Subunit structure	Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1 By similarity.
Tissue specificity	Ubiquitously expressed, with a higher expression observed in brain, spleen and liver. Isoform 1 and isoform 2 are considered agonist and isoform 3 and isoform 4 are considered antagonist.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. Contains 1 death domain. Contains 1 protein kinase domain.
Caution	Asn-333 is present instead of the conserved Asp which is expected to be an active site residue.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	interleukin-1-mediated signaling pathway Inferred from electronic annotation. Source: Compara lipopolysaccharide-mediated signaling pathway Inferred from direct assay Ref.2. Source: MGI negative regulation of NF-kappaB transcription factor activity Inferred from electronic annotation. Source: Compara negative regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.2. Source: MGI positive regulation of NF-kappaB transcription factor activity Inferred from direct assay Ref.2. Source: MGI regulation of cytokine-mediated signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from sequence orthology Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CFA1-1) Also known as: IRAK-2a; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CFA1-2) Also known as: IRAK-2b; The sequence of this isoform differs from the canonical sequence as follows: 94-141: Missing.

Isoform 3 (identifier: Q8CFA1-3) Also known as: IRAK-2d; The sequence of this isoform differs from the canonical sequence as follows: 32-93: ASYVITDLTQLRKIKSMERVQGVSITRELLWWWSMRQATVQQLVDLLCHLELYRAAQIVLSW → G 490-499: Missing.

Isoform 4 (identifier: Q8CFA1-4) Also known as: IRAK-2c; The sequence of this isoform differs from the canonical sequence as follows: 1-143: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 622

622

Interleukin-1 receptor-associated kinase-like 2

PRO_0000277560

Regions

Domain

13 – 94

82

Death

Domain

208 – 473

266

Protein kinase

Nucleotide binding

214 – 222

9

ATP By similarity

Nucleotide binding

335 – 338

4

ATP By similarity

Sites

Binding site

235

1

ATP By similarity

Natural variations

Alternative sequence

1 – 143

143

Missing in isoform 4.

VSP_023025

Alternative sequence

32 – 93

62

ASYVI…IVLSW → G in isoform 3.

VSP_023026

Alternative sequence

94 – 141

48

Missing in isoform 2.

VSP_023027

Alternative sequence

490 – 499

10

Missing in isoform 3.

VSP_023028

Experimental info

Sequence conflict

129

1

V → A in BAE28437. Ref.3

Sequence conflict

142 – 151

10

PIMAGAQRQR → ALLPSFLLLF in BAC28438. Ref.3

Sequence conflict

151

1

R → V in BAC37114. Ref.3

Sequence conflict

188 – 190

3

GLP → SLH in BAE28437. Ref.3

Sequence conflict

267

1

V → I in BAE28437. Ref.3

Sequence conflict

273

1

F → V in BAE28437. Ref.3

Sequence conflict

343

1

Q → R in BAC37114. Ref.3

Sequence conflict

345

1

L → F in AAH85324. Ref.4

Sequence conflict

438

1

C → G in BAE28437. Ref.3

Sequence conflict

448

1

V → E in AAO24761. Ref.2

Sequence conflict

448

1

V → E in AAO24762. Ref.2

Sequence conflict

448

1

V → E in AAO24763. Ref.2

Sequence conflict

448

1

V → E in AAO24764. Ref.2

Sequence conflict

459

1

K → R in BAE28437. Ref.3

Sequence conflict

509

1

W → G in AAH85324. Ref.4

Sequence conflict

535

1

S → G in BAC26088. Ref.3

Sequence conflict

554

1

F → S in BAE28437. Ref.3

Sequence conflict

585

1

N → D in BAE28437. Ref.3

Sequence conflict

599

1

K → E in BAE28437. Ref.3

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (IRAK-2a) [UniParc]. Last modified June 1, 2003. Version 2. Checksum: AD4692A0B6BFE733 Show »	FASTA	622	69,047
10 20 30 40 50 60 MACYIYQLPS WVLDDLCRNI DTLSEWDWMQ FASYVITDLT QLRKIKSMER VQGVSITREL 70 80 90 100 110 120 LWWWSMRQAT VQQLVDLLCH LELYRAAQIV LSWKPVPEST SPLPAFPEAV KPGAVATSRR 130 140 150 160 170 180 NLKDEQEKVR PVKPRSLLDT GPIMAGAQRQ RPCEMDAPCS LKTDAPDSPQ SKYCSTSTSA 190 200 210 220 230 240 PKQERLLGLP GDRLFWSEAD VVQATEDFDQ SHRISEGTFA DIYQGQRNGV AFAFKKLREV 250 260 270 280 290 300 AGSSPGSMDR FLQAEMQLCL RCCHANVLPL LGFCTGRQFH SLIYPYMANG SLHDRLWAQG 310 320 330 340 350 360 NSDMLPWPQR ASICSGLLLA VEHLHSLDII HSNVKSANVL LDQHLNPKLA HPVAHPHPDN 370 380 390 400 410 420 KKTKYTVMRT HLFQASAAYL PEHFIRVGQL TKQVDIFSCG IVLAEVLTGI PAMDKDRSPV 430 440 450 460 470 480 YLKDLLLSEI PNSTSSVCSR KTSMGKAVVK EICQRHVEKR AGLLPEACEE AWATAVSVCL 490 500 510 520 530 540 RRRNASVEEA RVSLAGVEEQ LRGQLSLPWS RVSEATGSSS NTPEETDDVD NSSLSVPSLV 550 560 570 580 590 600 MMASCPGAAS SPLFTGHGAA QPSTSGRQEA DSSSEACTGP QTPQNATETS WKIEINEAKR 610 620 RLMENIVLYK EERLDSSELF GP « Hide
Isoform 2 (IRAK-2b) [UniParc]. Checksum: EFA629937D2F6335 Show »	FASTA	574	63,870
10 20 30 40 50 60 MACYIYQLPS WVLDDLCRNI DTLSEWDWMQ FASYVITDLT QLRKIKSMER VQGVSITREL 70 80 90 100 110 120 LWWWSMRQAT VQQLVDLLCH LELYRAAQIV LSWPIMAGAQ RQRPCEMDAP CSLKTDAPDS 130 140 150 160 170 180 PQSKYCSTST SAPKQERLLG LPGDRLFWSE ADVVQATEDF DQSHRISEGT FADIYQGQRN 190 200 210 220 230 240 GVAFAFKKLR EVAGSSPGSM DRFLQAEMQL CLRCCHANVL PLLGFCTGRQ FHSLIYPYMA 250 260 270 280 290 300 NGSLHDRLWA QGNSDMLPWP QRASICSGLL LAVEHLHSLD IIHSNVKSAN VLLDQHLNPK 310 320 330 340 350 360 LAHPVAHPHP DNKKTKYTVM RTHLFQASAA YLPEHFIRVG QLTKQVDIFS CGIVLAEVLT 370 380 390 400 410 420 GIPAMDKDRS PVYLKDLLLS EIPNSTSSVC SRKTSMGKAV VKEICQRHVE KRAGLLPEAC 430 440 450 460 470 480 EEAWATAVSV CLRRRNASVE EARVSLAGVE EQLRGQLSLP WSRVSEATGS SSNTPEETDD 490 500 510 520 530 540 VDNSSLSVPS LVMMASCPGA ASSPLFTGHG AAQPSTSGRQ EADSSSEACT GPQTPQNATE 550 560 570 TSWKIEINEA KRRLMENIVL YKEERLDSSE LFGP « Hide
Isoform 3 (IRAK-2d) [UniParc]. Checksum: E4030CE5C6EB366A Show »	FASTA	551	60,734
10 20 30 40 50 60 MACYIYQLPS WVLDDLCRNI DTLSEWDWMQ FGKPVPESTS PLPAFPEAVK PGAVATSRRN 70 80 90 100 110 120 LKDEQEKVRP VKPRSLLDTG PIMAGAQRQR PCEMDAPCSL KTDAPDSPQS KYCSTSTSAP 130 140 150 160 170 180 KQERLLGLPG DRLFWSEADV VQATEDFDQS HRISEGTFAD IYQGQRNGVA FAFKKLREVA 190 200 210 220 230 240 GSSPGSMDRF LQAEMQLCLR CCHANVLPLL GFCTGRQFHS LIYPYMANGS LHDRLWAQGN 250 260 270 280 290 300 SDMLPWPQRA SICSGLLLAV EHLHSLDIIH SNVKSANVLL DQHLNPKLAH PVAHPHPDNK 310 320 330 340 350 360 KTKYTVMRTH LFQASAAYLP EHFIRVGQLT KQVDIFSCGI VLAEVLTGIP AMDKDRSPVY 370 380 390 400 410 420 LKDLLLSEIP NSTSSVCSRK TSMGKAVVKE ICQRHVEKRA GLLPEACEEA WATAVSVCLR 430 440 450 460 470 480 RRNASVEEQL RGQLSLPWSR VSEATGSSSN TPEETDDVDN SSLSVPSLVM MASCPGAASS 490 500 510 520 530 540 PLFTGHGAAQ PSTSGRQEAD SSSEACTGPQ TPQNATETSW KIEINEAKRR LMENIVLYKE 550 ERLDSSELFG P « Hide
Isoform 4 (IRAK-2c) [UniParc]. Checksum: A029C4439FAC23D2 Show »	FASTA	479	52,463
10 20 30 40 50 60 MAGAQRQRPC EMDAPCSLKT DAPDSPQSKY CSTSTSAPKQ ERLLGLPGDR LFWSEADVVQ 70 80 90 100 110 120 ATEDFDQSHR ISEGTFADIY QGQRNGVAFA FKKLREVAGS SPGSMDRFLQ AEMQLCLRCC 130 140 150 160 170 180 HANVLPLLGF CTGRQFHSLI YPYMANGSLH DRLWAQGNSD MLPWPQRASI CSGLLLAVEH 190 200 210 220 230 240 LHSLDIIHSN VKSANVLLDQ HLNPKLAHPV AHPHPDNKKT KYTVMRTHLF QASAAYLPEH 250 260 270 280 290 300 FIRVGQLTKQ VDIFSCGIVL AEVLTGIPAM DKDRSPVYLK DLLLSEIPNS TSSVCSRKTS 310 320 330 340 350 360 MGKAVVKEIC QRHVEKRAGL LPEACEEAWA TAVSVCLRRR NASVEEARVS LAGVEEQLRG 370 380 390 400 410 420 QLSLPWSRVS EATGSSSNTP EETDDVDNSS LSVPSLVMMA SCPGAASSPL FTGHGAAQPS 430 440 450 460 470 TSGRQEADSS SEACTGPQTP QNATETSWKI EINEAKRRLM ENIVLYKEER LDSSELFGP « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of murine IRAK-2." Rosati O., Martin M.U. Biochem. Biophys. Res. Commun. 297:52-58(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c.
[2]	"The murine IRAK2 gene encodes four alternatively spliced isoforms, two of which are inhibitory." Hardy M.P., O'Neill L.A. J. Biol. Chem. 279:27699-27708(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4). Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Bone marrow, Cecum, Medulla oblongata and Skin.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Trophoblast stem cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ440756 mRNA. Translation: CAD29447.2. AY162378 mRNA. Translation: AAO24761.1. AY162379 mRNA. Translation: AAO24762.1. AY162380 mRNA. Translation: AAO24763.1. AY162381 mRNA. Translation: AAO24764.1. AK028733 mRNA. Translation: BAC26088.1. AK033707 mRNA. Translation: BAC28438.1. AK078062 mRNA. Translation: BAC37114.1. AK148248 mRNA. Translation: BAE28437.1. AK150056 mRNA. Translation: BAE29271.1. AK151428 mRNA. Translation: BAE30392.1. AK152682 mRNA. Translation: BAE31414.1. AK154715 mRNA. Translation: BAE32783.1. BC085324 mRNA. Translation: AAH85324.1.
IPI	IPI00420934. IPI00468594. IPI00624206. IPI00828977.
RefSeq	NP_001107025.1. NM_001113553.1. NP_751893.3. NM_172161.4.
UniGene	Mm.152142.
3D structure databases
ProteinModelPortal	Q8CFA1.
SMR	Q8CFA1. Positions 2-94, 188-476.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-49685N.
IntAct	Q8CFA1. 1 interaction.
PTM databases
PhosphoSite	Q8CFA1.
Proteomic databases
PRIDE	Q8CFA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000059286; ENSMUSP00000055073; ENSMUSG00000060477. ENSMUST00000089022; ENSMUSP00000086416; ENSMUSG00000060477. ENSMUST00000089023; ENSMUSP00000086417; ENSMUSG00000060477. ENSMUST00000113024; ENSMUSP00000108647; ENSMUSG00000060477.
GeneID	108960.
KEGG	mmu:108960.
UCSC	uc009dhc.3. mouse. uc009dhe.3. mouse. uc012eqf.2. mouse.
Organism-specific databases
CTD	3656.
MGI	MGI:2429603. Irak2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00530000063073.
HOVERGEN	HBG052145.
InParanoid	Q8CFA1.
KO	K04731.
OMA	PWSGLSE.
OrthoDB	EOG4R23TT.
Gene expression databases
ArrayExpress	Q8CFA1.
Bgee	Q8CFA1.
CleanEx	MM_IRAK2.
Genevestigator	Q8CFA1.
Family and domain databases
Gene3D	1.10.533.10. 1 hit.
InterPro	IPR011029. DEATH-like_dom. IPR000488. Death_domain. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00531. Death. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50017. DEATH_DOMAIN. False negative. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	IRAK2. mouse.
NextBio	361524.
SOURCE	Search...

Entry information

Entry name

IRAK2_MOUSE

Accession

Primary (citable) accession number: Q8CFA1
Secondary accession number(s): Q3U3K2

Q8CEA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents