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Q8CF97

- VCIP1_RAT

UniProt

Q8CF97 - VCIP1_RAT

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Protein
Deubiquitinating protein VCIP135
Gene
Vcpip1, Vcip135
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151 By similarity
Active sitei218 – 2181Nucleophile By similarity
Active sitei353 – 3531 By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. Golgi organization Source: RGD
  2. Golgi reassembly Source: RGD
  3. endoplasmic reticulum membrane fusion Source: RGD
  4. mitotic nuclear division Source: RGD
  5. protein K11-linked deubiquitination Source: UniProtKB
  6. protein K48-linked deubiquitination Source: UniProtKB
  7. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC64.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Deubiquitinating protein VCIP135 (EC:3.4.19.12)
Alternative name(s):
Valosin-containing protein p97/p47 complex-interacting protein 1
Valosin-containing protein p97/p47 complex-interacting protein p135
Gene namesi
Name:Vcpip1
Synonyms:Vcip135
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi708520. Vcpip1.

Subcellular locationi

Endoplasmic reticulum. Golgi apparatusGolgi stack
Note: Associated with Golgi stacks and endoplasmic reticulum.1 Publication

GO - Cellular componenti

  1. Golgi stack Source: UniProtKB-SubCell
  2. endoplasmic reticulum Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi218 – 2181C → A or S: Loss of deubiquitinating activity and ability to promote VCP-mediated Golgi membrane fusion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12211221Deubiquitinating protein VCIP135
PRO_0000065771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei407 – 4071N6-acetyllysine By similarity
Modified residuei746 – 7461Phosphoserine By similarity
Modified residuei762 – 7621Phosphothreonine By similarity
Modified residuei993 – 9931Phosphoserine By similarity
Modified residuei1197 – 11971Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CF97.
PRIDEiQ8CF97.

PTM databases

PhosphoSiteiQ8CF97.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

GenevestigatoriQ8CF97.

Interactioni

Subunit structurei

Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP.

Structurei

3D structure databases

ProteinModelPortaliQ8CF97.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 360154OTU
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 2017Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 1 OTU domain.

Phylogenomic databases

eggNOGiNOG262027.
GeneTreeiENSGT00390000002854.
HOGENOMiHOG000154813.
HOVERGENiHBG059748.
InParanoidiQ8CF97.
KOiK11861.
OMAiQTAKKNP.
OrthoDBiEOG7FV3PG.
PhylomeDBiQ8CF97.
TreeFamiTF329469.

Family and domain databases

InterProiIPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CF97-1 [UniParc]FASTAAdd to Basket

« Hide

MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA TPGGLSKRRD RRILSGSCPD     50
PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN 100
ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD 150
MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA 200
NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA 250
RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR 300
PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG 350
RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG 400
DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE 450
EVTAAAKKAV MDNRLHKCLL CGALSELHVP PEWLAPGGKL YNLAKSTHGQ 500
LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGTSVRRVRG 550
DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR 600
VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI 650
LHQTAKKNPD DYTPVNIDGA HAQRIGDVQG QELESQLPTK IILTGQKTKT 700
LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI 750
RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KSSTTFFELQ 800
ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL 850
KGKAEGGPST AAHSAHTVRQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT 900
LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV 950
YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH 1000
GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSNPHM DPRARETLAV 1050
RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV 1100
VEAQRKKLQE MVSSIQASMD KHLRDQSTEQ TPSDLSQRKV EAVSSSVRPG 1150
NLQTGLPESF SLTGGTENLN TETTDSRVAD VLGAAFATRS KAQKENSMEE 1200
PEEMDSQDAE TTNTTEPMDH S 1221
Length:1,221
Mass (Da):134,566
Last modified:November 23, 2004 - v2
Checksum:i23487E853AD9FD4B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71P → L in AAQ14350. 1 Publication
Sequence conflicti158 – 1581D → N in AAQ14350. 1 Publication
Sequence conflicti603 – 6031R → K in AAQ14350. 1 Publication
Sequence conflicti652 – 6521H → L in AAQ14350. 1 Publication
Sequence conflicti675 – 6751I → V in AAQ14350. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB045378 mRNA. Translation: BAC44841.2.
AF289091 mRNA. Translation: AAQ14350.1.
RefSeqiNP_789827.3. NM_176857.3.
UniGeneiRn.204326.

Genome annotation databases

EnsembliENSRNOT00000009136; ENSRNOP00000009136; ENSRNOG00000006980.
GeneIDi286761.
KEGGirno:286761.
UCSCiRGD:708520. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB045378 mRNA. Translation: BAC44841.2 .
AF289091 mRNA. Translation: AAQ14350.1 .
RefSeqi NP_789827.3. NM_176857.3.
UniGenei Rn.204326.

3D structure databases

ProteinModelPortali Q8CF97.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C64.006.

PTM databases

PhosphoSitei Q8CF97.

Proteomic databases

PaxDbi Q8CF97.
PRIDEi Q8CF97.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009136 ; ENSRNOP00000009136 ; ENSRNOG00000006980 .
GeneIDi 286761.
KEGGi rno:286761.
UCSCi RGD:708520. rat.

Organism-specific databases

CTDi 80124.
RGDi 708520. Vcpip1.

Phylogenomic databases

eggNOGi NOG262027.
GeneTreei ENSGT00390000002854.
HOGENOMi HOG000154813.
HOVERGENi HBG059748.
InParanoidi Q8CF97.
KOi K11861.
OMAi QTAKKNP.
OrthoDBi EOG7FV3PG.
PhylomeDBi Q8CF97.
TreeFami TF329469.

Miscellaneous databases

NextBioi 624949.
PROi Q8CF97.

Gene expression databases

Genevestigatori Q8CF97.

Family and domain databases

InterProi IPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo."
    Uchiyama K., Jokitalo E., Kano F., Murata M., Zhang X., Canas B., Newman R., Rabouille C., Pappin D., Freemont P., Kondo H.
    J. Cell Biol. 159:855-866(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 870-878 AND 1095-1105, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VCP, IDENTIFICATION BY MASS SPECTROMETRY.
  2. Kondo H., Uchiyama K., Kondo H.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 7.
  3. "VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments."
    Wang Y., Satoh A., Warren G., Meyer H.H.
    J. Cell Biol. 164:973-978(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-218.
    Tissue: Liver.

Entry informationi

Entry nameiVCIP1_RAT
AccessioniPrimary (citable) accession number: Q8CF97
Secondary accession number(s): Q7TNK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: November 23, 2004
Last modified: September 3, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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