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Q8CF97

- VCIP1_RAT

UniProt

Q8CF97 - VCIP1_RAT

Protein

Deubiquitinating protein VCIP135

Gene

Vcpip1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.2 Publications

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei215 – 2151By similarity
    Active sitei218 – 2181NucleophileBy similarity
    Active sitei353 – 3531By similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. endoplasmic reticulum membrane fusion Source: RGD
    2. Golgi organization Source: RGD
    3. Golgi reassembly Source: RGD
    4. mitotic nuclear division Source: RGD
    5. protein K11-linked deubiquitination Source: UniProtKB
    6. protein K48-linked deubiquitination Source: UniProtKB
    7. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC64.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deubiquitinating protein VCIP135 (EC:3.4.19.12)
    Alternative name(s):
    Valosin-containing protein p97/p47 complex-interacting protein 1
    Valosin-containing protein p97/p47 complex-interacting protein p135
    Gene namesi
    Name:Vcpip1
    Synonyms:Vcip135
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi708520. Vcpip1.

    Subcellular locationi

    Endoplasmic reticulum 1 Publication. Golgi apparatusGolgi stack 1 Publication
    Note: Associated with Golgi stacks and endoplasmic reticulum.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. Golgi stack Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi218 – 2181C → A or S: Loss of deubiquitinating activity and ability to promote VCP-mediated Golgi membrane fusion. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12211221Deubiquitinating protein VCIP135PRO_0000065771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei407 – 4071N6-acetyllysineBy similarity
    Modified residuei746 – 7461PhosphoserineBy similarity
    Modified residuei762 – 7621PhosphothreonineBy similarity
    Modified residuei993 – 9931PhosphoserineBy similarity
    Modified residuei1197 – 11971PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8CF97.
    PRIDEiQ8CF97.

    PTM databases

    PhosphoSiteiQ8CF97.

    Expressioni

    Tissue specificityi

    Widely expressed.

    Gene expression databases

    GenevestigatoriQ8CF97.

    Interactioni

    Subunit structurei

    Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CF97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini207 – 360154OTUPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 2017Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG262027.
    GeneTreeiENSGT00390000002854.
    HOGENOMiHOG000154813.
    HOVERGENiHBG059748.
    InParanoidiQ8CF97.
    KOiK11861.
    OMAiQTAKKNP.
    OrthoDBiEOG7FV3PG.
    PhylomeDBiQ8CF97.
    TreeFamiTF329469.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CF97-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA TPGGLSKRRD RRILSGSCPD     50
    PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN 100
    ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD 150
    MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA 200
    NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA 250
    RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR 300
    PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG 350
    RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG 400
    DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE 450
    EVTAAAKKAV MDNRLHKCLL CGALSELHVP PEWLAPGGKL YNLAKSTHGQ 500
    LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGTSVRRVRG 550
    DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR 600
    VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI 650
    LHQTAKKNPD DYTPVNIDGA HAQRIGDVQG QELESQLPTK IILTGQKTKT 700
    LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI 750
    RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KSSTTFFELQ 800
    ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL 850
    KGKAEGGPST AAHSAHTVRQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT 900
    LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV 950
    YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH 1000
    GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSNPHM DPRARETLAV 1050
    RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV 1100
    VEAQRKKLQE MVSSIQASMD KHLRDQSTEQ TPSDLSQRKV EAVSSSVRPG 1150
    NLQTGLPESF SLTGGTENLN TETTDSRVAD VLGAAFATRS KAQKENSMEE 1200
    PEEMDSQDAE TTNTTEPMDH S 1221
    Length:1,221
    Mass (Da):134,566
    Last modified:November 23, 2004 - v2
    Checksum:i23487E853AD9FD4B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71P → L in AAQ14350. (PubMed:15037600)Curated
    Sequence conflicti158 – 1581D → N in AAQ14350. (PubMed:15037600)Curated
    Sequence conflicti603 – 6031R → K in AAQ14350. (PubMed:15037600)Curated
    Sequence conflicti652 – 6521H → L in AAQ14350. (PubMed:15037600)Curated
    Sequence conflicti675 – 6751I → V in AAQ14350. (PubMed:15037600)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB045378 mRNA. Translation: BAC44841.2.
    AF289091 mRNA. Translation: AAQ14350.1.
    RefSeqiNP_789827.3. NM_176857.3.
    UniGeneiRn.204326.

    Genome annotation databases

    EnsembliENSRNOT00000009136; ENSRNOP00000009136; ENSRNOG00000006980.
    GeneIDi286761.
    KEGGirno:286761.
    UCSCiRGD:708520. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB045378 mRNA. Translation: BAC44841.2 .
    AF289091 mRNA. Translation: AAQ14350.1 .
    RefSeqi NP_789827.3. NM_176857.3.
    UniGenei Rn.204326.

    3D structure databases

    ProteinModelPortali Q8CF97.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C64.006.

    PTM databases

    PhosphoSitei Q8CF97.

    Proteomic databases

    PaxDbi Q8CF97.
    PRIDEi Q8CF97.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000009136 ; ENSRNOP00000009136 ; ENSRNOG00000006980 .
    GeneIDi 286761.
    KEGGi rno:286761.
    UCSCi RGD:708520. rat.

    Organism-specific databases

    CTDi 80124.
    RGDi 708520. Vcpip1.

    Phylogenomic databases

    eggNOGi NOG262027.
    GeneTreei ENSGT00390000002854.
    HOGENOMi HOG000154813.
    HOVERGENi HBG059748.
    InParanoidi Q8CF97.
    KOi K11861.
    OMAi QTAKKNP.
    OrthoDBi EOG7FV3PG.
    PhylomeDBi Q8CF97.
    TreeFami TF329469.

    Miscellaneous databases

    NextBioi 624949.
    PROi Q8CF97.

    Gene expression databases

    Genevestigatori Q8CF97.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo."
      Uchiyama K., Jokitalo E., Kano F., Murata M., Zhang X., Canas B., Newman R., Rabouille C., Pappin D., Freemont P., Kondo H.
      J. Cell Biol. 159:855-866(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 870-878 AND 1095-1105, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VCP, IDENTIFICATION BY MASS SPECTROMETRY.
    2. Kondo H., Uchiyama K., Kondo H.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 7.
    3. "VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments."
      Wang Y., Satoh A., Warren G., Meyer H.H.
      J. Cell Biol. 164:973-978(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-218.
      Tissue: Liver.

    Entry informationi

    Entry nameiVCIP1_RAT
    AccessioniPrimary (citable) accession number: Q8CF97
    Secondary accession number(s): Q7TNK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3