Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 27

Gene

Usp27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinase that can reduce the levels of BCL2L11/BIM ubiquitination and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal. By acting on BCL2L11 levels, may counteract the anti-apoptotic effects of MAPK activity.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87NucleophilePROSITE-ProRule annotation1
Active sitei380Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • Lys48-specific deubiquitinase activity Source: UniProtKB
  • Lys63-specific deubiquitinase activity Source: UniProtKB
  • thiol-dependent ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  • histone deubiquitination Source: GO_Central
  • positive regulation of apoptotic process Source: UniProtKB
  • protein deubiquitination Source: UniProtKB
  • protein K48-linked deubiquitination Source: UniProtKB
  • protein K63-linked deubiquitination Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • regulation of transcription, DNA-templated Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 27 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 27
Ubiquitin thioesterase 27
Ubiquitin-specific-processing protease 27
X-linked ubiquitin carboxyl-terminal hydrolase 27
Gene namesi
Name:Usp27
Synonyms:Usp27x
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1859645. Usp27x.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87C → A: Loss of catalytic activity. No effect on BCL2L11-binding, BCL2L11 stabilization, nor on apoptosis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003675151 – 438Ubiquitin carboxyl-terminal hydrolase 27Add BLAST438

Proteomic databases

MaxQBiQ8CEG8.
PaxDbiQ8CEG8.
PRIDEiQ8CEG8.

PTM databases

iPTMnetiQ8CEG8.
PhosphoSitePlusiQ8CEG8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000046269.
GenevisibleiQ8CEG8. MM.

Interactioni

Subunit structurei

Interacts with phosphorylated BCL2L11 isoform BIMEL; this interaction leads to BCL2L11 deubiquitination and stabilization.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000111409.

Structurei

3D structure databases

ProteinModelPortaliQ8CEG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 421USPAdd BLAST344

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
GeneTreeiENSGT00860000133708.
HOVERGENiHBG058014.
InParanoidiQ8CEG8.
KOiK11366.
OMAiTEKHIHE.
OrthoDBiEOG091G09GI.
TreeFamiTF323554.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CEG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCKDYVYDID IEQIAKEEQG EALKLQASTS TEVSQQQCSV PGLGEKYPTW
60 70 80 90 100
ETTKPELELL GHNPRRRRIA SSFTIGLRGL INLGNTCFMN CIVQALTHTP
110 120 130 140 150
ILRDFFLSDR HRCEMPSPEL CLVCEMSSLF RELYSGNPSP HVPYKLLHLV
160 170 180 190 200
WIHARHLAGY RQQDAHEFLI AALDVLHRHC KGDDVGKVAS NPNHCNCIID
210 220 230 240 250
QIFTGGLQSD VTCQACHGVS TTIDPCWDIS LDLPGSCTSF WPMSPGRESS
260 270 280 290 300
LNGESHIPGI TTLTDCLRRF TRPEHLGSSA KIKCGSCQSY QESTKQLTMK
310 320 330 340 350
KLPVVACFHF KRFEHSAKQR RKITTYISFP LELDMTPFMA SSKETRVNGQ
360 370 380 390 400
LQLPTNSANN ENKYSLFAVV NHQGTLESGH YTSFIRHHRD QWFKCDDAVI
410 420 430
TKASIKDVLD SEGYLLFYHK QVLEPEPEKV KEMTPQAY
Length:438
Mass (Da):49,599
Last modified:March 24, 2009 - v2
Checksum:i159B156E0E00FEB9
GO

Sequence cautioni

The sequence AAF66953 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC25840 differs from that shown. Reason: Frameshift at position 369.Curated
The sequence BAC25840 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC26935 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74T → A in AAF66953 (PubMed:10857745).Curated1
Sequence conflicti192P → H in AAF66953 (PubMed:10857745).Curated1
Sequence conflicti251 – 252LN → VD in AAF66953 (PubMed:10857745).Curated2
Sequence conflicti324T → A in AAF66953 (PubMed:10857745).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229643 mRNA. Translation: AAF66953.1. Different initiation.
AK028249 mRNA. Translation: BAC25840.1. Sequence problems.
AK030383 mRNA. Translation: BAC26935.1. Different initiation.
AL663104 Genomic DNA. Translation: CAM17475.1.
CCDSiCCDS52983.1.
RefSeqiNP_062334.2. NM_019461.4.
UniGeneiMm.483107.
Mm.491689.

Genome annotation databases

EnsembliENSMUST00000115744; ENSMUSP00000111409; ENSMUSG00000046269.
ENSMUST00000178293; ENSMUSP00000137509; ENSMUSG00000046269.
ENSMUST00000191497; ENSMUSP00000139402; ENSMUSG00000046269.
GeneIDi54651.
KEGGimmu:54651.
UCSCiuc012hee.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229643 mRNA. Translation: AAF66953.1. Different initiation.
AK028249 mRNA. Translation: BAC25840.1. Sequence problems.
AK030383 mRNA. Translation: BAC26935.1. Different initiation.
AL663104 Genomic DNA. Translation: CAM17475.1.
CCDSiCCDS52983.1.
RefSeqiNP_062334.2. NM_019461.4.
UniGeneiMm.483107.
Mm.491689.

3D structure databases

ProteinModelPortaliQ8CEG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000111409.

PTM databases

iPTMnetiQ8CEG8.
PhosphoSitePlusiQ8CEG8.

Proteomic databases

MaxQBiQ8CEG8.
PaxDbiQ8CEG8.
PRIDEiQ8CEG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115744; ENSMUSP00000111409; ENSMUSG00000046269.
ENSMUST00000178293; ENSMUSP00000137509; ENSMUSG00000046269.
ENSMUST00000191497; ENSMUSP00000139402; ENSMUSG00000046269.
GeneIDi54651.
KEGGimmu:54651.
UCSCiuc012hee.1. mouse.

Organism-specific databases

CTDi389856.
MGIiMGI:1859645. Usp27x.

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
GeneTreeiENSGT00860000133708.
HOVERGENiHBG058014.
InParanoidiQ8CEG8.
KOiK11366.
OMAiTEKHIHE.
OrthoDBiEOG091G09GI.
TreeFamiTF323554.

Miscellaneous databases

PROiQ8CEG8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046269.
GenevisibleiQ8CEG8. MM.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP27_MOUSE
AccessioniPrimary (citable) accession number: Q8CEG8
Secondary accession number(s): B1ATV2, Q8BSW2, Q9JIG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although strongly related to USP22, which deubiquitinates histones, lacks the N-terminal UBP-type zinc finger, suggesting it does not have the ability to deubiquitinate histones.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.