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Protein

NF-kappa-B inhibitor-interacting Ras-like protein 1

Gene

Nkiras1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18GTPBy similarity8
Nucleotide bindingi61 – 65GTPBy similarity5
Nucleotide bindingi120 – 123GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor-interacting Ras-like protein 1
Alternative name(s):
I-kappa-B-interacting Ras-like protein 1
Short name:
Kappa B-Ras protein 1
Short name:
KappaB-Ras1
Gene namesi
Name:Nkiras1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1916971. Nkiras1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002256771 – 192NF-kappa-B inhibitor-interacting Ras-like protein 1Add BLAST192

Proteomic databases

MaxQBiQ8CEC5.
PaxDbiQ8CEC5.
PRIDEiQ8CEC5.

PTM databases

iPTMnetiQ8CEC5.
PhosphoSitePlusiQ8CEC5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021772.
CleanExiMM_NKIRAS1.
ExpressionAtlasiQ8CEC5. baseline and differential.
GenevisibleiQ8CEC5. MM.

Interactioni

Subunit structurei

Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo. Forms a complex with NFKBIB and NF-kappa-B heterodimer (p50/NFKB1 and p65/RELA). Also interacts with c-Rel (REL) (By similarity).By similarity

Protein-protein interaction databases

BioGridi213637. 4 interactors.
STRINGi10090.ENSMUSP00000121496.

Structurei

3D structure databases

ProteinModelPortaliQ8CEC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 93Interactions with NFKBIA and NFKBIBBy similarityAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 43Effector region9

Domaini

In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Leu residues, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3883. Eukaryota.
ENOG4110VES. LUCA.
GeneTreeiENSGT00390000006495.
HOVERGENiHBG105089.
InParanoidiQ8CEC5.
KOiK17197.
OMAiQWARGEK.
OrthoDBiEOG091G0IH8.
PhylomeDBiQ8CEC5.
TreeFamiTF314483.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CEC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGCKVVIC GLLSVGKTAI LEQLLYGNHT IGMEDCETLE DVYMASVETD
60 70 80 90 100
RGVKEQLHLY DTRGLQKGVE LPKHYFSFAD GFVLVYSVNN LESFQRVELL
110 120 130 140 150
KKEIDKFKDK KEVAIVVLGN KLDLSEQRQV DADVAQQWAR SEKVKLWEVT
160 170 180 190
VTDRRTLIEP FTLLASKLSQ PQSKSSFPLP GRKNKGNSNP EN
Length:192
Mass (Da):21,662
Last modified:March 1, 2003 - v1
Checksum:iD83192A7A3949E73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70E → D in AAF43150 (PubMed:10657303).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225707 mRNA. Translation: AAF43150.1.
AK028539 mRNA. Translation: BAC25998.1.
AK148040 mRNA. Translation: BAE28306.1.
AK167340 mRNA. Translation: BAE39441.1.
BC061092 mRNA. Translation: AAH61092.1.
CCDSiCCDS26836.1.
RefSeqiNP_001303640.1. NM_001316711.1.
NP_001303641.1. NM_001316712.1.
NP_076015.2. NM_023526.4.
XP_006518160.1. XM_006518097.3.
UniGeneiMm.25648.

Genome annotation databases

EnsembliENSMUST00000132374; ENSMUSP00000121496; ENSMUSG00000021772.
GeneIDi69721.
KEGGimmu:69721.
UCSCiuc007shs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225707 mRNA. Translation: AAF43150.1.
AK028539 mRNA. Translation: BAC25998.1.
AK148040 mRNA. Translation: BAE28306.1.
AK167340 mRNA. Translation: BAE39441.1.
BC061092 mRNA. Translation: AAH61092.1.
CCDSiCCDS26836.1.
RefSeqiNP_001303640.1. NM_001316711.1.
NP_001303641.1. NM_001316712.1.
NP_076015.2. NM_023526.4.
XP_006518160.1. XM_006518097.3.
UniGeneiMm.25648.

3D structure databases

ProteinModelPortaliQ8CEC5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213637. 4 interactors.
STRINGi10090.ENSMUSP00000121496.

PTM databases

iPTMnetiQ8CEC5.
PhosphoSitePlusiQ8CEC5.

Proteomic databases

MaxQBiQ8CEC5.
PaxDbiQ8CEC5.
PRIDEiQ8CEC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000132374; ENSMUSP00000121496; ENSMUSG00000021772.
GeneIDi69721.
KEGGimmu:69721.
UCSCiuc007shs.1. mouse.

Organism-specific databases

CTDi28512.
MGIiMGI:1916971. Nkiras1.

Phylogenomic databases

eggNOGiKOG3883. Eukaryota.
ENOG4110VES. LUCA.
GeneTreeiENSGT00390000006495.
HOVERGENiHBG105089.
InParanoidiQ8CEC5.
KOiK17197.
OMAiQWARGEK.
OrthoDBiEOG091G0IH8.
PhylomeDBiQ8CEC5.
TreeFamiTF314483.

Miscellaneous databases

PROiQ8CEC5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021772.
CleanExiMM_NKIRAS1.
ExpressionAtlasiQ8CEC5. baseline and differential.
GenevisibleiQ8CEC5. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKBRS1_MOUSE
AccessioniPrimary (citable) accession number: Q8CEC5
Secondary accession number(s): Q9JKV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.