ID   PUS7L_MOUSE             Reviewed;         702 AA.
AC   Q8CE46; Q6NY03; Q8C9X5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 138.
DE   RecName: Full=Pseudouridylate synthase PUS7L {ECO:0000305};
DE            EC=5.4.99.- {ECO:0000250|UniProtKB:Q9H0K6};
DE   AltName: Full=Pseudouridylate synthase 7 homolog-like protein {ECO:0000305};
GN   Name=Pus7l {ECO:0000312|MGI:MGI:1926145};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Skin, and Thymus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Pseudouridine synthase that catalyzes pseudouridylation of
CC       mRNAs. {ECO:0000250|UniProtKB:Q9H0K6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in mRNA = a pseudouridine in mRNA;
CC         Xref=Rhea:RHEA:56644, Rhea:RHEA-COMP:14658, Rhea:RHEA-COMP:14659,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000250|UniProtKB:Q9H0K6};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC30554.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact. May result from internal priming due to genomic poly-A tracts.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK029027; BAC26251.1; -; mRNA.
DR   EMBL; AK040257; BAC30554.1; ALT_SEQ; mRNA.
DR   EMBL; BC066798; AAH66798.1; -; mRNA.
DR   CCDS; CCDS27771.1; -.
DR   RefSeq; NP_766025.1; NM_172437.3.
DR   AlphaFoldDB; Q8CE46; -.
DR   SMR; Q8CE46; -.
DR   BioGRID; 219690; 2.
DR   STRING; 10090.ENSMUSP00000044075; -.
DR   iPTMnet; Q8CE46; -.
DR   PhosphoSitePlus; Q8CE46; -.
DR   EPD; Q8CE46; -.
DR   MaxQB; Q8CE46; -.
DR   PaxDb; 10090-ENSMUSP00000044075; -.
DR   PeptideAtlas; Q8CE46; -.
DR   ProteomicsDB; 301899; -.
DR   Pumba; Q8CE46; -.
DR   Antibodypedia; 25131; 67 antibodies from 15 providers.
DR   DNASU; 78895; -.
DR   Ensembl; ENSMUST00000049151.4; ENSMUSP00000044075.4; ENSMUSG00000033356.4.
DR   GeneID; 78895; -.
DR   KEGG; mmu:78895; -.
DR   UCSC; uc007xjh.2; mouse.
DR   AGR; MGI:1926145; -.
DR   CTD; 83448; -.
DR   MGI; MGI:1926145; Pus7l.
DR   VEuPathDB; HostDB:ENSMUSG00000033356; -.
DR   eggNOG; KOG2339; Eukaryota.
DR   GeneTree; ENSGT00530000063554; -.
DR   HOGENOM; CLU_005281_1_1_1; -.
DR   InParanoid; Q8CE46; -.
DR   OMA; IKIEDNH; -.
DR   OrthoDB; 168195at2759; -.
DR   PhylomeDB; Q8CE46; -.
DR   TreeFam; TF314278; -.
DR   BioGRID-ORCS; 78895; 2 hits in 79 CRISPR screens.
DR   PRO; PR:Q8CE46; -.
DR   Proteomes; UP000000589; Chromosome 15.
DR   RNAct; Q8CE46; Protein.
DR   Bgee; ENSMUSG00000033356; Expressed in ear vesicle and 168 other cell types or tissues.
DR   ExpressionAtlas; Q8CE46; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0009982; F:pseudouridine synthase activity; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; ISS:UniProtKB.
DR   GO; GO:0001522; P:pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02576; PseudoU_synth_ScPUS7; 1.
DR   Gene3D; 3.30.2350.20; TruD, catalytic domain; 2.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001656; PsdUridine_synth_TruD.
DR   InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR   InterPro; IPR042214; TruD_catalytic.
DR   NCBIfam; TIGR00094; tRNA_TruD_broad; 1.
DR   PANTHER; PTHR13326:SF21; PSEUDOURIDYLATE SYNTHASE PUS7L; 1.
DR   PANTHER; PTHR13326; TRNA PSEUDOURIDINE SYNTHASE D; 1.
DR   Pfam; PF01142; TruD; 1.
DR   PIRSF; PIRSF037016; Pseudouridin_synth_euk_prd; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS50984; TRUD; 1.
DR   Genevisible; Q8CE46; MM.
PE   1: Evidence at protein level;
KW   Isomerase; mRNA processing; Phosphoprotein; Reference proteome.
FT   CHAIN           1..702
FT                   /note="Pseudouridylate synthase PUS7L"
FT                   /id="PRO_0000316786"
FT   DOMAIN          424..646
FT                   /note="TRUD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00342"
FT   REGION          84..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        339
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q57261"
FT   MOD_RES         79
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H0K6"
FT   CONFLICT        151
FT                   /note="S -> P (in Ref. 2; AAH66798)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        558
FT                   /note="D -> N (in Ref. 2; AAH66798)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        667
FT                   /note="E -> G (in Ref. 2; AAH66798)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   702 AA;  79197 MW;  77D12455B78BCAEE CRC64;
     MAEDTDGGIR FNSLCFINDH VGFQGSIKTS PSDFIVIEID EQGQLVSKAT DGSLYEISKI
     QSEPSNSVKK PKLNIQNVSL EHKNSEGAAD LPGCSDGDRS HQSDSEKENS VNSVTSKCEE
     ESVDLLRSLL DEKTHTSLGQ FACDIKRMWN SQTELTEPSP ELSLGKILDK NRRAVLHSAV
     RQAFPFLITV GNQGEVVVKP NRECKELCRL VSEEEALGFF KYLDAKKENS KFTFKPDPNK
     DHRKAVHHFL NKKFGNLVET KSFPGQHHSA GNPDSAITVR FREKARGKRS HPEGCERGKA
     VYTAFTLQKE NLETFEAIGL LAVKLGVIPS DFSYAGLKDK RAITYQSMVV KKVTPERLKS
     IKEEIEKKRM NVFNIRSVGD CLRLGQLKGN HFEIIIRHLR NQLNDSANLT ERILEAIENV
     KNKGFVNYYG PQRFGKGQKI QTDQIGLALL KNEMVKAIKL FLTPEDVDDP VNKAKQYFLR
     TEDAKGTLSL MPEFRVRERA LLESLHRFGV TEEGCVRAWF SFPHSMRIFY IHAYSSRIWN
     EAASYRLAAY GPKVVEGDLI CSDEDADKHF PSSKVHLVTK EEESAHTYAL HQVVLPVLGY
     NVQYPENQVG RWYQEALSRD GLQACRFRVP ALKLNVPGCY RHIVKHPRNV SHRLVHPDPA
     TEEARVEGPH SDDTASSLSL SFDLDASCYA TVCLREMMKG DI
//