Reviewed, UniProtKB/Swiss-Prot Q8CE08 (PPAP_MOUSE)

Names and origin

Protein names

Recommended name:
    Prostatic acid phosphatase
    EC=3.1.3.2

Gene names

Name:

Acpp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Isoform 1: Secreted By similarity. Isoform 2: Lysosome membrane; Single-pass type I membrane protein By similarity. Note: Predominantly localized in the plasma membrane but also detected in intracellular vesicles By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CE08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CE08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     378-381: QGRN → QVLRVILATTFCLVTGILVILLLVLIRHGPCWQRDVYRNI

Note: Predominantly localized in the plasma membrane but also detected in intracellular vesicles (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 381	350	Prostatic acid phosphatase		PRO_0000356293
Sites
	Active site	43	1	Nucleophile By similarity
	Active site	289	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	332	1	N-linked (GlcNAc...) Potential
	Disulfide bond	160 ↔ 371		By similarity
	Disulfide bond	214 ↔ 312		By similarity
	Disulfide bond	346 ↔ 350		By similarity
Natural variations
	Alternative sequence	378 – 381	4	QGRN → QVLRVILATTFCLVTGILVI LLLVLIRHGPCWQRDVYRNI in isoform 2.		VSP_036024
Experimental info
	Sequence conflict	2	1	R → G in AAI39827. Ref.4
	Sequence conflict	3	1	A → S in BAC36318. Ref.2
	Sequence conflict	10	1	R → P in AAI39827. Ref.4
	Sequence conflict	145	1	V → L in BAC36318. Ref.2
	Sequence conflict	230	1	A → P in BAC36318. Ref.2
	Sequence conflict	264	1	N → H in BAC36318. Ref.2
	Sequence conflict	355	1	F → L in BAC36318. Ref.2
	Sequence conflict	357 – 358	2	EL → DV in AAF23171. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C0077819D77CB251	FASTA	381	43,717
	10 20 30 40 50 60 MRAVPLPLSR TASLSLGFLL LLSLCLDPGQ AKELKFVTLV FRHGDRGPIE TFPTDPITES 70 80 90 100 110 120 SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ IYIRSTDVDR TLMSAMTNLA 130 140 150 160 170 180 ALFPPEGISI WNPRLLWQPI PVHTVSLSED RLLYLPFRDC PRFEELKSET LESEEFLKRL 190 200 210 220 230 240 HPYKSFLDTL SSLSGFDDQD LFGIWSKVYD PLFCESVHNF TLPSWATEDA MIKLKELSEL 250 260 270 280 290 300 SLLSLYGIHK QKEKSRLQGG VLVNEILKNM KLATQPQKYK KLVMYSAHDT TVSGLQMALD 310 320 330 340 350 360 VYNGVLPPYA SCHMMELYHD KGGHFVEMYY RNETQNEPYP LTLPGCTHSC PLEKFAELLD 370 380 PVISQDWATE CMATSSHQGR N
	Isoform 2. Checksum: 505B9B551BA5CEF7	FASTA	417	47,863
	10 20 30 40 50 60 MRAVPLPLSR TASLSLGFLL LLSLCLDPGQ AKELKFVTLV FRHGDRGPIE TFPTDPITES 70 80 90 100 110 120 SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ IYIRSTDVDR TLMSAMTNLA 130 140 150 160 170 180 ALFPPEGISI WNPRLLWQPI PVHTVSLSED RLLYLPFRDC PRFEELKSET LESEEFLKRL 190 200 210 220 230 240 HPYKSFLDTL SSLSGFDDQD LFGIWSKVYD PLFCESVHNF TLPSWATEDA MIKLKELSEL 250 260 270 280 290 300 SLLSLYGIHK QKEKSRLQGG VLVNEILKNM KLATQPQKYK KLVMYSAHDT TVSGLQMALD 310 320 330 340 350 360 VYNGVLPPYA SCHMMELYHD KGGHFVEMYY RNETQNEPYP LTLPGCTHSC PLEKFAELLD 370 380 390 400 410 PVISQDWATE CMATSSHQVL RVILATTFCL VTGILVILLL VLIRHGPCWQ RDVYRNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of mouse prostatic acid phosphatase." Crew M.D., Chatta G.S., Borg C.D. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Head and Skin.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF210243 mRNA. Translation: AAF23171.1. AK029273 mRNA. Translation: BAC26366.1. AK076383 mRNA. Translation: BAC36318.1. CT030733 Genomic DNA. Translation: CAX15527.1. CT030733 Genomic DNA. Translation: CAX15528.1. BC139826 mRNA. Translation: AAI39827.1.
IPI	IPI00135015. IPI00410945.
RefSeq	NP_062781.2. NP_997551.1.
UniGene	Mm.19941
3D structure databases
HSSP	HSSP built from PDB template 1RPA based on UniProtKB P20646.
SMR	Q8CE08. Positions 32-373.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CE08.
Genome annotation databases
Ensembl	ENSMUST00000062723; ENSMUSP00000059889; ENSMUSG00000032561; Mus musculus. [Genome view] ENSMUST00000112590; ENSMUSP00000108209; ENSMUSG00000032561; Mus musculus. [Genome view]
GeneID	56318.
KEGG	mmu:56318.
UCSC	uc009rhl.1. mouse. uc009rhn.1. mouse.
Organism-specific databases
CTD	56318.
MGI	MGI:1928480. Acpp.
Phylogenomic databases
HOVERGEN	Q8CE08.
OMA	VHNFTLP.
Gene expression databases
ArrayExpress	Q8CE08.
Bgee	Q8CE08.
Genevestigator	Q8CE08.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	312276.
SOURCE	Search...

Entry information

Entry name

PPAP_MOUSE

Accession

Primary (citable) accession number: Q8CE08
Secondary accession number(s): A4QPG2 , B8JJZ5, B8JJZ6, Q8C682, Q9QXH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)