Q8CE08 (PPAP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostatic acid phosphatase
EC=3.1.3.2

Alternative name(s):
5'-nucleotidase
Short name=5'-NT
EC=3.1.3.5
Ecto-5'-nucleotidase
Fluoride-resistant acid phosphatase
Short name=FRAP
Thiamine monophosphatase
Short name=TMPase

Gene names

Name:

Acpp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma By similarity. Ref.6 Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Ref.6
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate. Ref.6 A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. Ref.6
Subunit structure	Homodimer; dimer formation is required for phosphatase activity By similarity.
Subcellular location	Isoform 1: Secreted By similarity Ref.5. Isoform 2: Cell membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles. Colocalizes with LAMP2 on the lysosome membrane By similarity. Ref.5
Tissue specificity	Isoform 1 is expressed in salivary gland, thymus and thyroid gland. Isoform 2 is widely expressed in prostate lobes, brain, kidney, liver, lung, muscle, placenta, salivary gland, spleen, thyroid and thymus. Locates to Schwann cells and fibroblasts. Expressed in peptidergic and non-peptidergic nociceptive (pain-sensing) neurons. Preferentially expressed in non-peptidergic doral root ganglia neurons. Ref.5 Ref.6 Ref.7
Disruption phenotype	Null mice display greater thermal hyperalgesia (pain sensitivity) and mechanical allodynia. No thiamine monophosphatase (TMPase) activity detected in dorsal root ganglion (DRG) neurons. Ref.6
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Lysosome Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenosine metabolic process Inferred from direct assay Ref.6. Source: UniProtKB nucleotide metabolic process Inferred from mutant phenotype Ref.6. Source: UniProtKB purine nucleobase metabolic process Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular_component	Golgi cisterna Inferred from electronic annotation. Source: Compara apical part of cell Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell filopodium Inferred from direct assay PubMed 17658863. Source: UniProtKB integral to membrane Inferred from direct assay PubMed 17658863. Source: UniProtKB lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell multivesicular body Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay PubMed 17658863. Source: UniProtKB secretory granule Inferred from electronic annotation. Source: Compara
Molecular_function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC acid phosphatase activity Inferred from sequence or structural similarity PubMed 2373368. Source: MGI choline binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CE08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CE08-2)

Also known as: TMPase; TM-PAP; cellular PAP; cPAP;

The sequence of this isoform differs from the canonical sequence as follows:
     378-381: QGRN → QVLRVILATTFCLVTGILVILLLVLIRHGPCWQRDVYRNI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 381	350	Prostatic acid phosphatase		PRO_0000356293
Sites
	Active site	43	1	Nucleophile By similarity
	Active site	289	1	Proton donor By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	46	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	288	1	Substrate By similarity
	Site	48	1	Important for substrate specificity By similarity
	Site	137	1	Required for dimerization By similarity
	Site	143	1	Required for dimerization By similarity
	Site	205	1	Required for structural stability By similarity
Amino acid modifications
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	332	1	N-linked (GlcNAc...) Potential
	Disulfide bond	160 ↔ 371		By similarity
	Disulfide bond	214 ↔ 312		By similarity
	Disulfide bond	346 ↔ 350		By similarity
Natural variations
	Alternative sequence	378 – 381	4	QGRN → QVLRVILATTFCLVTGILVI LLLVLIRHGPCWQRDVYRNI in isoform 2.		VSP_036024
Experimental info
	Sequence conflict	2	1	R → G in AAI39827. Ref.4
	Sequence conflict	3	1	A → S in BAC36318. Ref.2
	Sequence conflict	10	1	R → P in AAI39827. Ref.4
	Sequence conflict	145	1	V → L in BAC36318. Ref.2
	Sequence conflict	230	1	A → P in BAC36318. Ref.2
	Sequence conflict	264	1	N → H in BAC36318. Ref.2
	Sequence conflict	355	1	F → L in BAC36318. Ref.2
	Sequence conflict	357 – 358	2	EL → DV in AAF23171. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C0077819D77CB251	FASTA	381	43,717
	10 20 30 40 50 60 MRAVPLPLSR TASLSLGFLL LLSLCLDPGQ AKELKFVTLV FRHGDRGPIE TFPTDPITES 70 80 90 100 110 120 SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ IYIRSTDVDR TLMSAMTNLA 130 140 150 160 170 180 ALFPPEGISI WNPRLLWQPI PVHTVSLSED RLLYLPFRDC PRFEELKSET LESEEFLKRL 190 200 210 220 230 240 HPYKSFLDTL SSLSGFDDQD LFGIWSKVYD PLFCESVHNF TLPSWATEDA MIKLKELSEL 250 260 270 280 290 300 SLLSLYGIHK QKEKSRLQGG VLVNEILKNM KLATQPQKYK KLVMYSAHDT TVSGLQMALD 310 320 330 340 350 360 VYNGVLPPYA SCHMMELYHD KGGHFVEMYY RNETQNEPYP LTLPGCTHSC PLEKFAELLD 370 380 PVISQDWATE CMATSSHQGR N
	Isoform 2 (TMPase) (TM-PAP) (cellular PAP) (cPAP) [UniParc]. Checksum: 505B9B551BA5CEF7	FASTA	417	47,863
	10 20 30 40 50 60 MRAVPLPLSR TASLSLGFLL LLSLCLDPGQ AKELKFVTLV FRHGDRGPIE TFPTDPITES 70 80 90 100 110 120 SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ IYIRSTDVDR TLMSAMTNLA 130 140 150 160 170 180 ALFPPEGISI WNPRLLWQPI PVHTVSLSED RLLYLPFRDC PRFEELKSET LESEEFLKRL 190 200 210 220 230 240 HPYKSFLDTL SSLSGFDDQD LFGIWSKVYD PLFCESVHNF TLPSWATEDA MIKLKELSEL 250 260 270 280 290 300 SLLSLYGIHK QKEKSRLQGG VLVNEILKNM KLATQPQKYK KLVMYSAHDT TVSGLQMALD 310 320 330 340 350 360 VYNGVLPPYA SCHMMELYHD KGGHFVEMYY RNETQNEPYP LTLPGCTHSC PLEKFAELLD 370 380 390 400 410 PVISQDWATE CMATSSHQVL RVILATTFCL VTGILVILLL VLIRHGPCWQ RDVYRNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of mouse prostatic acid phosphatase." Crew M.D., Chatta G.S., Borg C.D. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Head and Skin.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"Prostatic acid phosphatase is not a prostate specific target." Quintero I.B., Araujo C.L., Pulkka A.E., Wirkkala R.S., Herrala A.M., Eskelinen E.-L., Jokitalo E., Hellstroem P.A., Tuominen H.J., Hirvikoski P.P., Vihko P.T. Cancer Res. 67:6549-6554(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Prostatic acid phosphatase is an ectonucleotidase and suppresses pain by generating adenosine." Zylka M.J., Sowa N.A., Taylor-Blake B., Twomey M.A., Herrala A., Voikar V., Vihko P. Neuron 60:111-122(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS AN ECTONUCLEOSIDASE, DISRUPTION PHENOTYPE, ENZYME ACTIVITY, TISSUE SPECIFICITY, FUNCTION.
[7]	"Prostatic acid phosphatase is expressed in peptidergic and nonpeptidergic nociceptive neurons of mice and rats." Taylor-Blake B., Zylka M.J. PLoS ONE 5:E8674-E8674(2010) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF210243 mRNA. Translation: AAF23171.1. AK029273 mRNA. Translation: BAC26366.1. AK076383 mRNA. Translation: BAC36318.1. CT030733 Genomic DNA. Translation: CAX15527.1. CT030733 Genomic DNA. Translation: CAX15528.1. BC139826 mRNA. Translation: AAI39827.1.
IPI	IPI00135015. IPI00410945.
RefSeq	NP_062781.2. NM_019807.2. NP_997551.1. NM_207668.2.
UniGene	Mm.19941.
3D structure databases
HSSP	HSSP built from PDB template 1RPA based on UniProtKB P20646.
ProteinModelPortal	Q8CE08.
SMR	Q8CE08. Positions 32-373.
ModBase	Search...
PTM databases
PhosphoSite	Q8CE08.
Proteomic databases
PRIDE	Q8CE08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000062723; ENSMUSP00000059889; ENSMUSG00000032561. ENSMUST00000112590; ENSMUSP00000108209; ENSMUSG00000032561.
GeneID	56318.
KEGG	mmu:56318.
UCSC	uc009rhl.1. mouse. uc009rhm.1. mouse.
Organism-specific databases
CTD	55.
MGI	MGI:1928480. Acpp.
Phylogenomic databases
eggNOG	NOG85977.
GeneTree	ENSGT00530000062956.
HOGENOM	HOG000231439.
HOVERGEN	HBG002203.
InParanoid	Q8C682.
KO	K14410.
OMA	FTLPSWA.
Gene expression databases
Bgee	Q8CE08.
Genevestigator	Q8CE08.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. [Graphical view]
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	312276.
SOURCE	Search...

Entry information

Entry name

PPAP_MOUSE

Accession

Primary (citable) accession number: Q8CE08
Secondary accession number(s): A4QPG2 , B8JJZ5, B8JJZ6, Q8C682, Q9QXH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program