Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic carboxypeptidase 3

Gene

Agbl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metallocarboxypeptidase that mediates both deglutamylation and deaspartylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates or polyaspartates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.2 Publications

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi368 – 3681ZincBy similarity
Metal bindingi371 – 3711ZincBy similarity
Active sitei418 – 4181NucleophileBy similarity
Metal bindingi464 – 4641ZincBy similarity

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein side chain deglutamylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM14.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic carboxypeptidase 3 (EC:3.4.17.-)
Alternative name(s):
ATP/GTP-binding protein-like 3
Gene namesi
Name:Agbl3
Synonyms:Ccp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1923473. Agbl3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

AGBL2 and AGBL3 double knockout mutant mice are viable and display no obvious phenotypic alterations. Show an increase in tubulin and MYLK polyglutamylation in sperm.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi540 – 5401E → A: Abolishes deglutamylase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10061006Cytosolic carboxypeptidase 3PRO_0000283752Add
BLAST

Proteomic databases

PaxDbiQ8CDP0.
PRIDEiQ8CDP0.

PTM databases

iPTMnetiQ8CDP0.
PhosphoSiteiQ8CDP0.

Expressioni

Tissue specificityi

Widely expressed. Expressed abundantly in tissues with m otile cilia such as testis, lung and trachea. Abundantly expressed in pituitary and kidney, moderately expressed in brain, eye, fat, pancreas, stomach, and adrenal.2 Publications

Inductioni

Up-regulated during ciliogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000038836.
CleanExiMM_AGBL3.
ExpressionAtlasiQ8CDP0. baseline and differential.
GenevisibleiQ8CDP0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110669.

Structurei

3D structure databases

ProteinModelPortaliQ8CDP0.
SMRiQ8CDP0. Positions 173-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Phylogenomic databases

eggNOGiKOG1814. Eukaryota.
KOG3641. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00550000074405.
HOGENOMiHOG000111052.
HOVERGENiHBG070840.
InParanoidiQ8CDP0.
OMAiTQEILQY.
OrthoDBiEOG091G0LY8.
PhylomeDBiQ8CDP0.
TreeFamiTF313794.

Family and domain databases

InterProiIPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CDP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEDSEEEDY SDRSISDDDD LDEDSFMKFV SDDIHPCTLL AADSIGDPFF
60 70 80 90 100
PRTTQILLEY QLGRWVPRLR GPRDLYGVSS SGPLSPTRWP YHCEVIDEKV
110 120 130 140 150
QHIEWTPFVP EPVYVPTGLE IEPVYPNSKE DTVVYLAEDD HLCKAYKEPC
160 170 180 190 200
FVYSRVGGNR TSLKQPVDNC DNTLVFEARF ESGNLQKVVK VADHEYELTV
210 220 230 240 250
RPDLFTNKHT QWYYFQVTNT QAEIVYRFTI VNFTKPASLY NRGMKPLFYS
260 270 280 290 300
EKEAKTHNIG WQRIGDQIKY YKNNLGQDGR HFFSLTWTFQ FPHSQDTCYF
310 320 330 340 350
AHCYPYTYSN LQEYLSGINS DPVRSKFCKI RVLCHTLARN MVYVLTITTP
360 370 380 390 400
LKTSDSKRKA VILTARVHPG ETNSSWIMKG FLDYILGDSS DARLLRDTFI
410 420 430 440 450
FKVVPMLNPD GVIVGNYRCS LAGRDLNRNY TSLLKESFPS VWYTRNMINR
460 470 480 490 500
LMEKREVILY CDLHGHSRKQ NIFMYGCDGS SRSKTKGLYL QQRIFPLMLS
510 520 530 540 550
KNCPNIFSFS ACKFNVQKSK EGTGRVVMWK MGIRNSFTLE ATFCGSTLGN
560 570 580 590 600
KRGTHFGTKD LESMGYHFCD SLLDYCDPDR SKYYQCLKEL EEMEKHLSSE
610 620 630 640 650
RVSDNTDTSL VEISLDVESS SRGSDSSESN DTQTYLLKVT SQARNKKKYL
660 670 680 690 700
KTKRERNAIL ANCQNNMQEV YGKEHLLQRH DESNSDGNDP RIDAPDVYVA
710 720 730 740 750
HCFRRPLPNQ GVVKIPGQRF YPGKTWSSSQ RMIKSLNKDH RTCILETCKN
760 770 780 790 800
PIQEVQSRGI NIHESCFKMA KCPMNKRPSH WIEKTRIPTE SHHQLKSKAK
810 820 830 840 850
RCSSFQSKRT GTNWTDDEKR IYRDKRIAQT QEILKYLLPI VESSQNRKST
860 870 880 890 900
QMNNLINPIA NLQQHQLIPT ACINRRRYSI PWTPTRNLPF KAQRNLMTDT
910 920 930 940 950
SEWLQSVPLG SFESLLPLCN LQKKTKHFEL WGKKAKDVQL ATSQWEAVPL
960 970 980 990 1000
SSNMDASIIR GNSVLQPKEF TMRSSKQRIP YLTKTSKKPS ESDGLLTFQL

KIHRNS
Length:1,006
Mass (Da):116,378
Last modified:March 1, 2003 - v1
Checksum:iA7E0C781E00CDCBF
GO
Isoform 2 (identifier: Q8CDP0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-144: Missing.
     867-1006: Missing.

Show »
Length:861
Mass (Da):99,726
Checksum:iCDFF376F68FF4F9E
GO
Isoform 3 (identifier: Q8CDP0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-144: Missing.

Show »
Length:1,001
Mass (Da):115,781
Checksum:iE009654CC22D8CBD
GO
Isoform 4 (identifier: Q8CDP0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-144: Missing.
     507-516: FSFSACKFNV → VRTFKTLTSL
     517-1006: Missing.

Show »
Length:511
Mass (Da):59,300
Checksum:iDFFB02BEAE5713BB
GO
Isoform 5 (identifier: Q8CDP0-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-129: EWTPFVPEPVYVPTGLEIEPVYPNSK → GMFLEVLGIQTLANLSILMLMRTLMM
     130-1006: Missing.

Show »
Length:129
Mass (Da):14,775
Checksum:iB191E07E6B61962C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei104 – 12926EWTPF…YPNSK → GMFLEVLGIQTLANLSILML MRTLMM in isoform 5. 1 PublicationVSP_024374Add
BLAST
Alternative sequencei130 – 1006877Missing in isoform 5. 1 PublicationVSP_024375Add
BLAST
Alternative sequencei140 – 1445Missing in isoform 2, isoform 3 and isoform 4. 3 PublicationsVSP_024376
Alternative sequencei507 – 51610FSFSACKFNV → VRTFKTLTSL in isoform 4. 2 PublicationsVSP_024377
Alternative sequencei517 – 1006490Missing in isoform 4. 2 PublicationsVSP_024378Add
BLAST
Alternative sequencei867 – 1006140Missing in isoform 2. 1 PublicationVSP_024379Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ867031 mRNA. Translation: ABI51950.1.
DQ867032 mRNA. Translation: ABI51951.1.
AK029793 mRNA. Translation: BAC26620.1.
AK030378 mRNA. Translation: BAC26932.1.
BC028521 mRNA. Translation: AAH28521.1.
BC087897 mRNA. Translation: AAH87897.1.
BC139065 mRNA. Translation: AAI39066.1.
CCDSiCCDS19995.2. [Q8CDP0-3]
CCDS80515.1. [Q8CDP0-1]
CCDS80516.1. [Q8CDP0-5]
RefSeqiNP_001276585.1. NM_001289656.1. [Q8CDP0-1]
NP_001276586.1. NM_001289657.1.
NP_001276587.1. NM_001289658.1. [Q8CDP0-5]
NP_848745.2. NM_178630.4. [Q8CDP0-3]
UniGeneiMm.150047.

Genome annotation databases

EnsembliENSMUST00000115014; ENSMUSP00000110666; ENSMUSG00000038836. [Q8CDP0-5]
ENSMUST00000115016; ENSMUSP00000110668; ENSMUSG00000038836. [Q8CDP0-1]
ENSMUST00000115017; ENSMUSP00000110669; ENSMUSG00000038836. [Q8CDP0-3]
GeneIDi76223.
KEGGimmu:76223.
UCSCiuc009bhm.2. mouse. [Q8CDP0-5]
uc009bhp.1. mouse. [Q8CDP0-4]
uc009bhq.1. mouse. [Q8CDP0-2]
uc009bhr.2. mouse. [Q8CDP0-3]
uc009bhs.2. mouse. [Q8CDP0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ867031 mRNA. Translation: ABI51950.1.
DQ867032 mRNA. Translation: ABI51951.1.
AK029793 mRNA. Translation: BAC26620.1.
AK030378 mRNA. Translation: BAC26932.1.
BC028521 mRNA. Translation: AAH28521.1.
BC087897 mRNA. Translation: AAH87897.1.
BC139065 mRNA. Translation: AAI39066.1.
CCDSiCCDS19995.2. [Q8CDP0-3]
CCDS80515.1. [Q8CDP0-1]
CCDS80516.1. [Q8CDP0-5]
RefSeqiNP_001276585.1. NM_001289656.1. [Q8CDP0-1]
NP_001276586.1. NM_001289657.1.
NP_001276587.1. NM_001289658.1. [Q8CDP0-5]
NP_848745.2. NM_178630.4. [Q8CDP0-3]
UniGeneiMm.150047.

3D structure databases

ProteinModelPortaliQ8CDP0.
SMRiQ8CDP0. Positions 173-478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110669.

Protein family/group databases

MEROPSiM14.026.

PTM databases

iPTMnetiQ8CDP0.
PhosphoSiteiQ8CDP0.

Proteomic databases

PaxDbiQ8CDP0.
PRIDEiQ8CDP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115014; ENSMUSP00000110666; ENSMUSG00000038836. [Q8CDP0-5]
ENSMUST00000115016; ENSMUSP00000110668; ENSMUSG00000038836. [Q8CDP0-1]
ENSMUST00000115017; ENSMUSP00000110669; ENSMUSG00000038836. [Q8CDP0-3]
GeneIDi76223.
KEGGimmu:76223.
UCSCiuc009bhm.2. mouse. [Q8CDP0-5]
uc009bhp.1. mouse. [Q8CDP0-4]
uc009bhq.1. mouse. [Q8CDP0-2]
uc009bhr.2. mouse. [Q8CDP0-3]
uc009bhs.2. mouse. [Q8CDP0-1]

Organism-specific databases

CTDi340351.
MGIiMGI:1923473. Agbl3.

Phylogenomic databases

eggNOGiKOG1814. Eukaryota.
KOG3641. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00550000074405.
HOGENOMiHOG000111052.
HOVERGENiHBG070840.
InParanoidiQ8CDP0.
OMAiTQEILQY.
OrthoDBiEOG091G0LY8.
PhylomeDBiQ8CDP0.
TreeFamiTF313794.

Miscellaneous databases

PROiQ8CDP0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038836.
CleanExiMM_AGBL3.
ExpressionAtlasiQ8CDP0. baseline and differential.
GenevisibleiQ8CDP0. MM.

Family and domain databases

InterProiIPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPC3_MOUSE
AccessioniPrimary (citable) accession number: Q8CDP0
Secondary accession number(s): B2RSY8
, Q09M06, Q09M07, Q8BSW4, Q8R036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.