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Q8CDG3

- VCIP1_MOUSE

UniProt

Q8CDG3 - VCIP1_MOUSE

Protein

Deubiquitinating protein VCIP135

Gene

Vcpip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei215 – 2151By similarity
    Active sitei218 – 2181NucleophileBy similarity
    Active sitei353 – 3531By similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. endoplasmic reticulum membrane fusion Source: Ensembl
    2. Golgi reassembly Source: Ensembl
    3. mitotic nuclear division Source: Ensembl
    4. protein K11-linked deubiquitination Source: UniProtKB
    5. protein K48-linked deubiquitination Source: UniProtKB
    6. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC64.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deubiquitinating protein VCIP135 (EC:3.4.19.12)
    Alternative name(s):
    Valosin-containing protein p97/p47 complex-interacting protein 1
    Valosin-containing protein p97/p47 complex-interacting protein p135
    Gene namesi
    Name:Vcpip1
    Synonyms:Vcip135
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1917925. Vcpip1.

    Subcellular locationi

    Endoplasmic reticulum By similarity. Golgi apparatusGolgi stack By similarity
    Note: Associated with Golgi stacks and endoplasmic reticulum.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. Golgi stack Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12201220Deubiquitinating protein VCIP135PRO_0000065770Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei407 – 4071N6-acetyllysineBy similarity
    Modified residuei746 – 7461PhosphoserineBy similarity
    Modified residuei762 – 7621PhosphothreonineBy similarity
    Cross-linki869 – 869Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei993 – 9931PhosphoserineBy similarity
    Modified residuei1196 – 11961PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ8CDG3.
    PaxDbiQ8CDG3.
    PRIDEiQ8CDG3.

    PTM databases

    PhosphoSiteiQ8CDG3.

    Expressioni

    Gene expression databases

    BgeeiQ8CDG3.
    GenevestigatoriQ8CDG3.

    Interactioni

    Subunit structurei

    Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP.By similarity

    Protein-protein interaction databases

    BioGridi214195. 1 interaction.
    IntActiQ8CDG3. 3 interactions.
    MINTiMINT-4139929.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CDG3.
    SMRiQ8CDG3. Positions 190-370, 776-848.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini207 – 360154OTUPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 2017Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG262027.
    GeneTreeiENSGT00390000002854.
    HOVERGENiHBG059748.
    InParanoidiQ8CDG3.
    KOiK11861.
    OrthoDBiEOG7FV3PG.
    PhylomeDBiQ8CDG3.
    TreeFamiTF329469.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8CDG3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA SPGGLSKRRD RRILSGSCPD     50
    PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN 100
    ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD 150
    MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA 200
    NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA 250
    RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR 300
    PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG 350
    RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG 400
    DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE 450
    EVTAAAKKAV MDNRLHKCLL YGALSELHVP SEWLAPGGKL YNLAKSTHGQ 500
    LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGSSVRRVRG 550
    DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR 600
    VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI 650
    LHQTAKKNPD DYTPVNIDGA HAQRVGDVQG QELESQLPTK IILTGQKTKT 700
    LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI 750
    RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KPSTTFFELQ 800
    ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL 850
    KGRAEGGPST AAHSAHTVKQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT 900
    LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV 950
    YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH 1000
    GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSHPHI DPRARETLAV 1050
    RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV 1100
    VEAQRKKLQE MVSSIQASMD KHLRDQSAEQ APSDLSQRKV EVVSSVRPVN 1150
    LQTGLPEPFS LTGGTENLNT ETTDSHVADV LGAAFATRSK AQKENSMEEP 1200
    EEMDSQDAET TNTTEPMDHS 1220
    Length:1,220
    Mass (Da):134,503
    Last modified:March 1, 2003 - v1
    Checksum:i8062E3C17DCD2E3B
    GO
    Isoform 2 (identifier: Q8CDG3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         904-906: EDV → KSN
         907-1220: Missing.

    Note: May be due to an intron retention. No experimental confirmation available.

    Show »
    Length:906
    Mass (Da):100,374
    Checksum:i700778A8E7031FFE
    GO

    Sequence cautioni

    The sequence AAH52908.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti471 – 4711Y → C in AAH59209. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei904 – 9063EDV → KSN in isoform 2. 1 PublicationVSP_009376
    Alternative sequencei907 – 1220314Missing in isoform 2. 1 PublicationVSP_009377Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030104 mRNA. Translation: BAC26787.1.
    AK077494 mRNA. Translation: BAC36830.1.
    BC052908 mRNA. Translation: AAH52908.1. Different initiation.
    BC059209 mRNA. Translation: AAH59209.1.
    CCDSiCCDS14814.1. [Q8CDG3-1]
    RefSeqiNP_775619.2. NM_173443.2.
    UniGeneiMm.274493.
    Mm.440951.

    Genome annotation databases

    EnsembliENSMUST00000057438; ENSMUSP00000051248; ENSMUSG00000045210.
    GeneIDi70675.
    KEGGimmu:70675.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030104 mRNA. Translation: BAC26787.1 .
    AK077494 mRNA. Translation: BAC36830.1 .
    BC052908 mRNA. Translation: AAH52908.1 . Different initiation.
    BC059209 mRNA. Translation: AAH59209.1 .
    CCDSi CCDS14814.1. [Q8CDG3-1 ]
    RefSeqi NP_775619.2. NM_173443.2.
    UniGenei Mm.274493.
    Mm.440951.

    3D structure databases

    ProteinModelPortali Q8CDG3.
    SMRi Q8CDG3. Positions 190-370, 776-848.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 214195. 1 interaction.
    IntActi Q8CDG3. 3 interactions.
    MINTi MINT-4139929.

    Protein family/group databases

    MEROPSi C64.006.

    PTM databases

    PhosphoSitei Q8CDG3.

    Proteomic databases

    MaxQBi Q8CDG3.
    PaxDbi Q8CDG3.
    PRIDEi Q8CDG3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000057438 ; ENSMUSP00000051248 ; ENSMUSG00000045210 .
    GeneIDi 70675.
    KEGGi mmu:70675.

    Organism-specific databases

    CTDi 80124.
    MGIi MGI:1917925. Vcpip1.

    Phylogenomic databases

    eggNOGi NOG262027.
    GeneTreei ENSGT00390000002854.
    HOVERGENi HBG059748.
    InParanoidi Q8CDG3.
    KOi K11861.
    OrthoDBi EOG7FV3PG.
    PhylomeDBi Q8CDG3.
    TreeFami TF329469.

    Miscellaneous databases

    NextBioi 332059.
    PROi Q8CDG3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CDG3.
    Genevestigatori Q8CDG3.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Embryo and Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Brain and Hematopoietic.

    Entry informationi

    Entry nameiVCIP1_MOUSE
    AccessioniPrimary (citable) accession number: Q8CDG3
    Secondary accession number(s): Q7TMU9, Q8BP90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3