Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Deubiquitinating protein VCIP135

Gene

Vcpip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215By similarity1
Active sitei218NucleophileBy similarity1
Active sitei353By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deubiquitinating protein VCIP135 (EC:3.4.19.12)
Alternative name(s):
Valosin-containing protein p97/p47 complex-interacting protein 1
Valosin-containing protein p97/p47 complex-interacting protein p135
Gene namesi
Name:Vcpip1
Synonyms:Vcip135
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917925. Vcpip1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657701 – 1220Deubiquitinating protein VCIP135Add BLAST1220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei407N6-acetyllysineBy similarity1
Modified residuei746PhosphoserineBy similarity1
Modified residuei756PhosphoserineBy similarity1
Modified residuei762PhosphothreonineBy similarity1
Modified residuei767PhosphoserineBy similarity1
Modified residuei993PhosphoserineBy similarity1
Modified residuei997PhosphoserineBy similarity1
Modified residuei1076PhosphoserineBy similarity1
Modified residuei1196PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CDG3.
PaxDbiQ8CDG3.
PeptideAtlasiQ8CDG3.
PRIDEiQ8CDG3.

PTM databases

iPTMnetiQ8CDG3.
PhosphoSitePlusiQ8CDG3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045210.

Interactioni

Subunit structurei

Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP.By similarity

Protein-protein interaction databases

BioGridi214195. 8 interactors.
IntActiQ8CDG3. 13 interactors.
MINTiMINT-4139929.
STRINGi10090.ENSMUSP00000051248.

Structurei

3D structure databases

ProteinModelPortaliQ8CDG3.
SMRiQ8CDG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 360OTUPROSITE-ProRule annotationAdd BLAST154

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 20Pro-richAdd BLAST17

Sequence similaritiesi

Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQZJ. Eukaryota.
ENOG410ZMR9. LUCA.
HOVERGENiHBG059748.
InParanoidiQ8CDG3.
KOiK11861.
PhylomeDBiQ8CDG3.
TreeFamiTF329469.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CDG3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA SPGGLSKRRD RRILSGSCPD
60 70 80 90 100
PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN
110 120 130 140 150
ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD
160 170 180 190 200
MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA
210 220 230 240 250
NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA
260 270 280 290 300
RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR
310 320 330 340 350
PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG
360 370 380 390 400
RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG
410 420 430 440 450
DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE
460 470 480 490 500
EVTAAAKKAV MDNRLHKCLL YGALSELHVP SEWLAPGGKL YNLAKSTHGQ
510 520 530 540 550
LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGSSVRRVRG
560 570 580 590 600
DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR
610 620 630 640 650
VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI
660 670 680 690 700
LHQTAKKNPD DYTPVNIDGA HAQRVGDVQG QELESQLPTK IILTGQKTKT
710 720 730 740 750
LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI
760 770 780 790 800
RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KPSTTFFELQ
810 820 830 840 850
ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL
860 870 880 890 900
KGRAEGGPST AAHSAHTVKQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT
910 920 930 940 950
LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV
960 970 980 990 1000
YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH
1010 1020 1030 1040 1050
GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSHPHI DPRARETLAV
1060 1070 1080 1090 1100
RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV
1110 1120 1130 1140 1150
VEAQRKKLQE MVSSIQASMD KHLRDQSAEQ APSDLSQRKV EVVSSVRPVN
1160 1170 1180 1190 1200
LQTGLPEPFS LTGGTENLNT ETTDSHVADV LGAAFATRSK AQKENSMEEP
1210 1220
EEMDSQDAET TNTTEPMDHS
Length:1,220
Mass (Da):134,503
Last modified:March 1, 2003 - v1
Checksum:i8062E3C17DCD2E3B
GO
Isoform 2 (identifier: Q8CDG3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-906: EDV → KSN
     907-1220: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:906
Mass (Da):100,374
Checksum:i700778A8E7031FFE
GO

Sequence cautioni

The sequence AAH52908 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti471Y → C in AAH59209 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009376904 – 906EDV → KSN in isoform 2. 1 Publication3
Alternative sequenceiVSP_009377907 – 1220Missing in isoform 2. 1 PublicationAdd BLAST314

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030104 mRNA. Translation: BAC26787.1.
AK077494 mRNA. Translation: BAC36830.1.
BC052908 mRNA. Translation: AAH52908.1. Different initiation.
BC059209 mRNA. Translation: AAH59209.1.
CCDSiCCDS14814.1. [Q8CDG3-1]
RefSeqiNP_775619.2. NM_173443.3.
UniGeneiMm.274493.
Mm.440951.

Genome annotation databases

GeneIDi70675.
KEGGimmu:70675.
UCSCiuc007ags.3. mouse. [Q8CDG3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030104 mRNA. Translation: BAC26787.1.
AK077494 mRNA. Translation: BAC36830.1.
BC052908 mRNA. Translation: AAH52908.1. Different initiation.
BC059209 mRNA. Translation: AAH59209.1.
CCDSiCCDS14814.1. [Q8CDG3-1]
RefSeqiNP_775619.2. NM_173443.3.
UniGeneiMm.274493.
Mm.440951.

3D structure databases

ProteinModelPortaliQ8CDG3.
SMRiQ8CDG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214195. 8 interactors.
IntActiQ8CDG3. 13 interactors.
MINTiMINT-4139929.
STRINGi10090.ENSMUSP00000051248.

PTM databases

iPTMnetiQ8CDG3.
PhosphoSitePlusiQ8CDG3.

Proteomic databases

EPDiQ8CDG3.
PaxDbiQ8CDG3.
PeptideAtlasiQ8CDG3.
PRIDEiQ8CDG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70675.
KEGGimmu:70675.
UCSCiuc007ags.3. mouse. [Q8CDG3-1]

Organism-specific databases

CTDi80124.
MGIiMGI:1917925. Vcpip1.

Phylogenomic databases

eggNOGiENOG410IQZJ. Eukaryota.
ENOG410ZMR9. LUCA.
HOVERGENiHBG059748.
InParanoidiQ8CDG3.
KOiK11861.
PhylomeDBiQ8CDG3.
TreeFamiTF329469.

Miscellaneous databases

PROiQ8CDG3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045210.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVCIP1_MOUSE
AccessioniPrimary (citable) accession number: Q8CDG3
Secondary accession number(s): Q7TMU9, Q8BP90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.