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Q8CDG3 (VCIP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deubiquitinating protein VCIP135

EC=3.4.19.12
Alternative name(s):
Valosin-containing protein p97/p47 complex-interacting protein 1
Valosin-containing protein p97/p47 complex-interacting protein p135
Gene names
Name:Vcpip1
Synonyms:Vcip135
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1220 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP By similarity.

Subcellular location

Endoplasmic reticulum By similarity. Golgi apparatusGolgi stack By similarity. Note: Associated with Golgi stacks and endoplasmic reticulum By similarity.

Sequence similarities

Contains 1 OTU domain.

Sequence caution

The sequence AAH52908.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CDG3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CDG3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     904-906: EDV → KSN
     907-1220: Missing.
Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12201220Deubiquitinating protein VCIP135
PRO_0000065770

Regions

Domain207 – 360154OTU
Compositional bias4 – 2017Pro-rich

Sites

Active site2151 By similarity
Active site2181Nucleophile By similarity
Active site3531 By similarity

Amino acid modifications

Modified residue4071N6-acetyllysine By similarity
Modified residue7461Phosphoserine By similarity
Modified residue7621Phosphothreonine By similarity
Modified residue9931Phosphoserine By similarity
Modified residue11961Phosphoserine By similarity
Cross-link869Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence904 – 9063EDV → KSN in isoform 2.
VSP_009376
Alternative sequence907 – 1220314Missing in isoform 2.
VSP_009377

Experimental info

Sequence conflict4711Y → C in AAH59209. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 8062E3C17DCD2E3B

FASTA1,220134,503
        10         20         30         40         50         60 
MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA SPGGLSKRRD RRILSGSCPD PKCQARLFFP 

        70         80         90        100        110        120 
ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN ALLGVTGAPK KNTELVKVMG 

       130        140        150        160        170        180 
LSNYHCKLLS PILARYGMDK QTGRAKLLRD MNQGELFDCA LLGDRAFLIE PEHVNTVGYG 

       190        200        210        220        230        240 
KDRSGSLLYL HDTLEDIKRA NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN 

       250        260        270        280        290        300 
LKQHFQQHLA RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR 

       310        320        330        340        350        360 
PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG RNHYIPLVGI 

       370        380        390        400        410        420 
KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG DRSLQDKYLL RLVAAMEEVF 

       430        440        450        460        470        480 
MDKHGIHPSL VADVHQYFYR RTGVIGVQPE EVTAAAKKAV MDNRLHKCLL YGALSELHVP 

       490        500        510        520        530        540 
SEWLAPGGKL YNLAKSTHGQ LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC 

       550        560        570        580        590        600 
HGSSVRRVRG DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR 

       610        620        630        640        650        660 
VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI LHQTAKKNPD 

       670        680        690        700        710        720 
DYTPVNIDGA HAQRVGDVQG QELESQLPTK IILTGQKTKT LHKEELNMSK TERTIQQNIT 

       730        740        750        760        770        780 
EQASVMQKRK TEKLKQEQKG QPRTVSPSTI RDGPSSAPAT PTKAPYSPTT SKEKKIRITT 

       790        800        810        820        830        840 
NDGRQSMVTL KPSTTFFELQ ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ 

       850        860        870        880        890        900 
HGDRITIEIL KGRAEGGPST AAHSAHTVKQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT 

       910        920        930        940        950        960 
LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV YNASEDRLEL 

       970        980        990       1000       1010       1020 
CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH GLLKLGSGGV VKKKSEQLHN 

      1030       1040       1050       1060       1070       1080 
VTAFQGKGHS LGTASSHPHI DPRARETLAV RKHNTGTDFS NSSIKTEPPV FTAASSNSEL 

      1090       1100       1110       1120       1130       1140 
IRIAPGVVTM RDGRQIDPDV VEAQRKKLQE MVSSIQASMD KHLRDQSAEQ APSDLSQRKV 

      1150       1160       1170       1180       1190       1200 
EVVSSVRPVN LQTGLPEPFS LTGGTENLNT ETTDSHVADV LGAAFATRSK AQKENSMEEP 

      1210       1220 
EEMDSQDAET TNTTEPMDHS 

« Hide

Isoform 2 [UniParc].

Checksum: 700778A8E7031FFE
Show »

FASTA906100,374

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryo and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain and Hematopoietic.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK030104 mRNA. Translation: BAC26787.1.
AK077494 mRNA. Translation: BAC36830.1.
BC052908 mRNA. Translation: AAH52908.1. Different initiation.
BC059209 mRNA. Translation: AAH59209.1.
RefSeqNP_775619.2. NM_173443.2.
UniGeneMm.274493.
Mm.440951.

3D structure databases

ProteinModelPortalQ8CDG3.
SMRQ8CDG3. Positions 157-420, 775-858.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid214195. 1 interaction.
IntActQ8CDG3. 3 interactions.
MINTMINT-4139929.

Protein family/group databases

MEROPSC64.006.

PTM databases

PhosphoSiteQ8CDG3.

Proteomic databases

PaxDbQ8CDG3.
PRIDEQ8CDG3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000057438; ENSMUSP00000051248; ENSMUSG00000045210.
GeneID70675.
KEGGmmu:70675.

Organism-specific databases

CTD80124.
MGIMGI:1917925. Vcpip1.

Phylogenomic databases

eggNOGNOG262027.
GeneTreeENSGT00390000002854.
HOVERGENHBG059748.
InParanoidQ8CDG3.
KOK11861.
OrthoDBEOG7FV3PG.
PhylomeDBQ8CDG3.
TreeFamTF329469.

Gene expression databases

BgeeQ8CDG3.
GenevestigatorQ8CDG3.

Family and domain databases

InterProIPR003323. OTU.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio332059.
PROQ8CDG3.
SOURCESearch...

Entry information

Entry nameVCIP1_MOUSE
AccessionPrimary (citable) accession number: Q8CDG3
Secondary accession number(s): Q7TMU9, Q8BP90
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot