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Q8CDG3

- VCIP1_MOUSE

UniProt

Q8CDG3 - VCIP1_MOUSE

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Protein

Deubiquitinating protein VCIP135

Gene
Vcpip1, Vcip135
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151 By similarity
Active sitei218 – 2181Nucleophile By similarity
Active sitei353 – 3531 By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. endoplasmic reticulum membrane fusion Source: Ensembl
  2. Golgi reassembly Source: Ensembl
  3. mitotic nuclear division Source: Ensembl
  4. protein K11-linked deubiquitination Source: UniProtKB
  5. protein K48-linked deubiquitination Source: UniProtKB
  6. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC64.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Deubiquitinating protein VCIP135 (EC:3.4.19.12)
Alternative name(s):
Valosin-containing protein p97/p47 complex-interacting protein 1
Valosin-containing protein p97/p47 complex-interacting protein p135
Gene namesi
Name:Vcpip1
Synonyms:Vcip135
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1917925. Vcpip1.

Subcellular locationi

Endoplasmic reticulum By similarity. Golgi apparatusGolgi stack By similarity
Note: Associated with Golgi stacks and endoplasmic reticulum By similarity.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
  2. Golgi stack Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12201220Deubiquitinating protein VCIP135PRO_0000065770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei407 – 4071N6-acetyllysine By similarity
Modified residuei746 – 7461Phosphoserine By similarity
Modified residuei762 – 7621Phosphothreonine By similarity
Cross-linki869 – 869Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei993 – 9931Phosphoserine By similarity
Modified residuei1196 – 11961Phosphoserine By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8CDG3.
PaxDbiQ8CDG3.
PRIDEiQ8CDG3.

PTM databases

PhosphoSiteiQ8CDG3.

Expressioni

Gene expression databases

BgeeiQ8CDG3.
GenevestigatoriQ8CDG3.

Interactioni

Subunit structurei

Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP By similarity.

Protein-protein interaction databases

BioGridi214195. 1 interaction.
IntActiQ8CDG3. 3 interactions.
MINTiMINT-4139929.

Structurei

3D structure databases

ProteinModelPortaliQ8CDG3.
SMRiQ8CDG3. Positions 190-370, 776-848.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 360154OTUAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 2017Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 OTU domain.

Phylogenomic databases

eggNOGiNOG262027.
GeneTreeiENSGT00390000002854.
HOVERGENiHBG059748.
InParanoidiQ8CDG3.
KOiK11861.
OrthoDBiEOG7FV3PG.
PhylomeDBiQ8CDG3.
TreeFamiTF329469.

Family and domain databases

InterProiIPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CDG3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA SPGGLSKRRD RRILSGSCPD     50
PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN 100
ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD 150
MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA 200
NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA 250
RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR 300
PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG 350
RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG 400
DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE 450
EVTAAAKKAV MDNRLHKCLL YGALSELHVP SEWLAPGGKL YNLAKSTHGQ 500
LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGSSVRRVRG 550
DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR 600
VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI 650
LHQTAKKNPD DYTPVNIDGA HAQRVGDVQG QELESQLPTK IILTGQKTKT 700
LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI 750
RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KPSTTFFELQ 800
ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL 850
KGRAEGGPST AAHSAHTVKQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT 900
LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV 950
YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH 1000
GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSHPHI DPRARETLAV 1050
RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV 1100
VEAQRKKLQE MVSSIQASMD KHLRDQSAEQ APSDLSQRKV EVVSSVRPVN 1150
LQTGLPEPFS LTGGTENLNT ETTDSHVADV LGAAFATRSK AQKENSMEEP 1200
EEMDSQDAET TNTTEPMDHS 1220
Length:1,220
Mass (Da):134,503
Last modified:March 1, 2003 - v1
Checksum:i8062E3C17DCD2E3B
GO
Isoform 2 (identifier: Q8CDG3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-906: EDV → KSN
     907-1220: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Show »
Length:906
Mass (Da):100,374
Checksum:i700778A8E7031FFE
GO

Sequence cautioni

The sequence AAH52908.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei904 – 9063EDV → KSN in isoform 2. VSP_009376
Alternative sequencei907 – 1220314Missing in isoform 2. VSP_009377Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti471 – 4711Y → C in AAH59209. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030104 mRNA. Translation: BAC26787.1.
AK077494 mRNA. Translation: BAC36830.1.
BC052908 mRNA. Translation: AAH52908.1. Different initiation.
BC059209 mRNA. Translation: AAH59209.1.
CCDSiCCDS14814.1. [Q8CDG3-1]
RefSeqiNP_775619.2. NM_173443.2.
UniGeneiMm.274493.
Mm.440951.

Genome annotation databases

EnsembliENSMUST00000057438; ENSMUSP00000051248; ENSMUSG00000045210.
GeneIDi70675.
KEGGimmu:70675.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030104 mRNA. Translation: BAC26787.1 .
AK077494 mRNA. Translation: BAC36830.1 .
BC052908 mRNA. Translation: AAH52908.1 . Different initiation.
BC059209 mRNA. Translation: AAH59209.1 .
CCDSi CCDS14814.1. [Q8CDG3-1 ]
RefSeqi NP_775619.2. NM_173443.2.
UniGenei Mm.274493.
Mm.440951.

3D structure databases

ProteinModelPortali Q8CDG3.
SMRi Q8CDG3. Positions 190-370, 776-848.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 214195. 1 interaction.
IntActi Q8CDG3. 3 interactions.
MINTi MINT-4139929.

Protein family/group databases

MEROPSi C64.006.

PTM databases

PhosphoSitei Q8CDG3.

Proteomic databases

MaxQBi Q8CDG3.
PaxDbi Q8CDG3.
PRIDEi Q8CDG3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000057438 ; ENSMUSP00000051248 ; ENSMUSG00000045210 .
GeneIDi 70675.
KEGGi mmu:70675.

Organism-specific databases

CTDi 80124.
MGIi MGI:1917925. Vcpip1.

Phylogenomic databases

eggNOGi NOG262027.
GeneTreei ENSGT00390000002854.
HOVERGENi HBG059748.
InParanoidi Q8CDG3.
KOi K11861.
OrthoDBi EOG7FV3PG.
PhylomeDBi Q8CDG3.
TreeFami TF329469.

Miscellaneous databases

NextBioi 332059.
PROi Q8CDG3.
SOURCEi Search...

Gene expression databases

Bgeei Q8CDG3.
Genevestigatori Q8CDG3.

Family and domain databases

InterProi IPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Brain and Hematopoietic.

Entry informationi

Entry nameiVCIP1_MOUSE
AccessioniPrimary (citable) accession number: Q8CDG3
Secondary accession number(s): Q7TMU9, Q8BP90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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