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Q8CDG3

- VCIP1_MOUSE

UniProt

Q8CDG3 - VCIP1_MOUSE

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Protein

Deubiquitinating protein VCIP135

Gene

Vcpip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151By similarity
Active sitei218 – 2181NucleophileBy similarity
Active sitei353 – 3531By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. endoplasmic reticulum membrane fusion Source: Ensembl
  2. Golgi reassembly Source: Ensembl
  3. mitotic nuclear division Source: Ensembl
  4. protein K11-linked deubiquitination Source: UniProtKB
  5. protein K48-linked deubiquitination Source: UniProtKB
  6. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC64.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Deubiquitinating protein VCIP135 (EC:3.4.19.12)
Alternative name(s):
Valosin-containing protein p97/p47 complex-interacting protein 1
Valosin-containing protein p97/p47 complex-interacting protein p135
Gene namesi
Name:Vcpip1
Synonyms:Vcip135
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1917925. Vcpip1.

Subcellular locationi

Endoplasmic reticulum By similarity. Golgi apparatusGolgi stack By similarity
Note: Associated with Golgi stacks and endoplasmic reticulum.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12201220Deubiquitinating protein VCIP135PRO_0000065770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei407 – 4071N6-acetyllysineBy similarity
Modified residuei746 – 7461PhosphoserineBy similarity
Modified residuei762 – 7621PhosphothreonineBy similarity
Cross-linki869 – 869Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei993 – 9931PhosphoserineBy similarity
Modified residuei1196 – 11961PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8CDG3.
PaxDbiQ8CDG3.
PRIDEiQ8CDG3.

PTM databases

PhosphoSiteiQ8CDG3.

Expressioni

Gene expression databases

BgeeiQ8CDG3.
GenevestigatoriQ8CDG3.

Interactioni

Subunit structurei

Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP.By similarity

Protein-protein interaction databases

BioGridi214195. 1 interaction.
IntActiQ8CDG3. 3 interactions.
MINTiMINT-4139929.

Structurei

3D structure databases

ProteinModelPortaliQ8CDG3.
SMRiQ8CDG3. Positions 190-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 360154OTUPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 2017Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG262027.
GeneTreeiENSGT00390000002854.
HOVERGENiHBG059748.
InParanoidiQ8CDG3.
KOiK11861.
OrthoDBiEOG7FV3PG.
PhylomeDBiQ8CDG3.
TreeFamiTF329469.

Family and domain databases

InterProiIPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CDG3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA SPGGLSKRRD RRILSGSCPD
60 70 80 90 100
PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN
110 120 130 140 150
ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD
160 170 180 190 200
MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA
210 220 230 240 250
NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA
260 270 280 290 300
RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR
310 320 330 340 350
PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG
360 370 380 390 400
RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG
410 420 430 440 450
DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE
460 470 480 490 500
EVTAAAKKAV MDNRLHKCLL YGALSELHVP SEWLAPGGKL YNLAKSTHGQ
510 520 530 540 550
LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGSSVRRVRG
560 570 580 590 600
DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR
610 620 630 640 650
VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI
660 670 680 690 700
LHQTAKKNPD DYTPVNIDGA HAQRVGDVQG QELESQLPTK IILTGQKTKT
710 720 730 740 750
LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI
760 770 780 790 800
RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KPSTTFFELQ
810 820 830 840 850
ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL
860 870 880 890 900
KGRAEGGPST AAHSAHTVKQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT
910 920 930 940 950
LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV
960 970 980 990 1000
YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH
1010 1020 1030 1040 1050
GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSHPHI DPRARETLAV
1060 1070 1080 1090 1100
RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV
1110 1120 1130 1140 1150
VEAQRKKLQE MVSSIQASMD KHLRDQSAEQ APSDLSQRKV EVVSSVRPVN
1160 1170 1180 1190 1200
LQTGLPEPFS LTGGTENLNT ETTDSHVADV LGAAFATRSK AQKENSMEEP
1210 1220
EEMDSQDAET TNTTEPMDHS
Length:1,220
Mass (Da):134,503
Last modified:March 1, 2003 - v1
Checksum:i8062E3C17DCD2E3B
GO
Isoform 2 (identifier: Q8CDG3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-906: EDV → KSN
     907-1220: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Show »
Length:906
Mass (Da):100,374
Checksum:i700778A8E7031FFE
GO

Sequence cautioni

The sequence AAH52908.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti471 – 4711Y → C in AAH59209. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei904 – 9063EDV → KSN in isoform 2. 1 PublicationVSP_009376
Alternative sequencei907 – 1220314Missing in isoform 2. 1 PublicationVSP_009377Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030104 mRNA. Translation: BAC26787.1.
AK077494 mRNA. Translation: BAC36830.1.
BC052908 mRNA. Translation: AAH52908.1. Different initiation.
BC059209 mRNA. Translation: AAH59209.1.
CCDSiCCDS14814.1. [Q8CDG3-1]
RefSeqiNP_775619.2. NM_173443.2.
UniGeneiMm.274493.
Mm.440951.

Genome annotation databases

EnsembliENSMUST00000057438; ENSMUSP00000051248; ENSMUSG00000045210.
GeneIDi70675.
KEGGimmu:70675.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030104 mRNA. Translation: BAC26787.1 .
AK077494 mRNA. Translation: BAC36830.1 .
BC052908 mRNA. Translation: AAH52908.1 . Different initiation.
BC059209 mRNA. Translation: AAH59209.1 .
CCDSi CCDS14814.1. [Q8CDG3-1 ]
RefSeqi NP_775619.2. NM_173443.2.
UniGenei Mm.274493.
Mm.440951.

3D structure databases

ProteinModelPortali Q8CDG3.
SMRi Q8CDG3. Positions 190-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 214195. 1 interaction.
IntActi Q8CDG3. 3 interactions.
MINTi MINT-4139929.

Protein family/group databases

MEROPSi C64.006.

PTM databases

PhosphoSitei Q8CDG3.

Proteomic databases

MaxQBi Q8CDG3.
PaxDbi Q8CDG3.
PRIDEi Q8CDG3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000057438 ; ENSMUSP00000051248 ; ENSMUSG00000045210 .
GeneIDi 70675.
KEGGi mmu:70675.

Organism-specific databases

CTDi 80124.
MGIi MGI:1917925. Vcpip1.

Phylogenomic databases

eggNOGi NOG262027.
GeneTreei ENSGT00390000002854.
HOVERGENi HBG059748.
InParanoidi Q8CDG3.
KOi K11861.
OrthoDBi EOG7FV3PG.
PhylomeDBi Q8CDG3.
TreeFami TF329469.

Miscellaneous databases

NextBioi 332059.
PROi Q8CDG3.
SOURCEi Search...

Gene expression databases

Bgeei Q8CDG3.
Genevestigatori Q8CDG3.

Family and domain databases

InterProi IPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Brain and Hematopoietic.

Entry informationi

Entry nameiVCIP1_MOUSE
AccessioniPrimary (citable) accession number: Q8CDG3
Secondary accession number(s): Q7TMU9, Q8BP90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3