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Protein

SPARC-related modular calcium-binding protein 2

Gene

Smoc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can stimulate endothelial cell proliferation, migration, as well as angiogenesis (By similarity). Promotes matrix assembly and cell adhesiveness.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi360 – 3711PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi397 – 4082PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • glycosaminoglycan binding Source: MGI
  • heparin binding Source: MGI

GO - Biological processi

  • extracellular matrix organization Source: MGI
  • positive regulation of cell-substrate adhesion Source: MGI
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC-related modular calcium-binding protein 2
Alternative name(s):
Secreted modular calcium-binding protein 2
Short name:
SMOC-2
Gene namesi
Name:Smoc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1929881. Smoc2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • interstitial matrix Source: MGI
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002031922 – 447SPARC-related modular calcium-binding protein 2Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi90 ↔ 113By similarity
Disulfide bondi124 ↔ 131By similarity
Disulfide bondi133 ↔ 153By similarity
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi216 ↔ 240By similarity
Disulfide bondi251 ↔ 258By similarity
Disulfide bondi260 ↔ 281By similarity
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8CD91.
PRIDEiQ8CD91.

Expressioni

Tissue specificityi

Strongly expressed in ovary, followed by heart, muscle, spleen, brain, thymus, lung, liver, kidney, spleen, testis, ovary and skeletal muscle.1 Publication

Developmental stagei

At E16.5, present in rib cartilage, choroid plexus epithelium and associated blood vessels, and developing oral and tooth germ epithelia (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000023886.
CleanExiMM_SMOC2.
GenevisibleiQ8CD91. MM.

Interactioni

Subunit structurei

Binds various proteins from the extracellular matrix.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024660.

Structurei

3D structure databases

ProteinModelPortaliQ8CD91.
SMRiQ8CD91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 86Kazal-likePROSITE-ProRule annotationAdd BLAST53
Domaini87 – 153Thyroglobulin type-1 1PROSITE-ProRule annotationAdd BLAST67
Domaini213 – 281Thyroglobulin type-1 2PROSITE-ProRule annotationAdd BLAST69
Domaini347 – 382EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini384 – 419EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 2 thyroglobulin type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4578. Eukaryota.
ENOG410YP7C. LUCA.
GeneTreeiENSGT00390000018436.
HOGENOMiHOG000234328.
HOVERGENiHBG058558.
InParanoidiQ8CD91.
OMAiPRCPGSI.
OrthoDBiEOG091G083O.
PhylomeDBiQ8CD91.
TreeFamiTF320666.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CD91-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPPQLCWLP LLAALLPPVP AQKFSALTFL RVDQDKDRDC SLDCPSSPQK
60 70 80 90 100
PLCASDGRTF LSRCEFQRAK CKDPQLEIAH RGNCKDVSRC VAERKYTQEQ
110 120 130 140 150
ARKEFQQVFI PECNDDGTYS QVQCHSYTGY CWCVTPNGRP ISGTAVAHKT
160 170 180 190 200
PRCPGSINEK VPQREGAGKA DDAAAPALET QPQGDEEDIA SRYPTLWTEQ
210 220 230 240 250
VKSRQNKTNK NSASSCDQEH QSALEEAKQP KNDNVVIPEC AHGGLYKPVQ
260 270 280 290 300
CHPSTGYCWC VLVDTGRPIP GTSTRYEQPK CDNTARAHPA KARDLYKNRP
310 320 330 340 350
LQGCPGAKKH EFLTSVLDAL STDMVHAVSD PSSSSGRLSE PDPSHTLEER
360 370 380 390 400
VVHWYFKLLD KNSSGDIGKK EIKPFKRFLR KKSKPKKCVK KFVEYCDMNN
410 420 430 440
DKSITVQELM GCLGVTREEG KANTRKRHTP RGNAESSSSN RQPRKQG
Length:447
Mass (Da):49,891
Last modified:March 1, 2003 - v1
Checksum:i26AB802F9D13A00C
GO
Isoform 2 (identifier: Q8CD91-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-336: MLPPQLCWLP...AVSDPSSSSG → MLLFLDLSPGL

Note: No experimental confirmation available.
Show »
Length:122
Mass (Da):14,077
Checksum:iCBD913F9F984CF06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti351V → D in BAB24753 (PubMed:16141072).Curated1
Sequence conflicti437 – 438SS → T (PubMed:12741954).Curated2
Sequence conflicti439S → F in BAB24753 (PubMed:16141072).Curated1
Sequence conflicti440N → Y in AAH19527 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0087231 – 336MLPPQ…SSSSG → MLLFLDLSPGL in isoform 2. 1 PublicationAdd BLAST336

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030958 mRNA. Translation: BAC27193.1.
AK006809 mRNA. Translation: BAB24753.1.
AJ249901 mRNA. Translation: CAC10355.1.
BC019527 mRNA. Translation: AAH19527.1.
CCDSiCCDS37442.1. [Q8CD91-1]
RefSeqiNP_071710.2. NM_022315.2. [Q8CD91-1]
UniGeneiMm.30162.

Genome annotation databases

EnsembliENSMUST00000024660; ENSMUSP00000024660; ENSMUSG00000023886. [Q8CD91-1]
GeneIDi64074.
KEGGimmu:64074.
UCSCiuc008amy.2. mouse. [Q8CD91-1]
uc008ana.2. mouse. [Q8CD91-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030958 mRNA. Translation: BAC27193.1.
AK006809 mRNA. Translation: BAB24753.1.
AJ249901 mRNA. Translation: CAC10355.1.
BC019527 mRNA. Translation: AAH19527.1.
CCDSiCCDS37442.1. [Q8CD91-1]
RefSeqiNP_071710.2. NM_022315.2. [Q8CD91-1]
UniGeneiMm.30162.

3D structure databases

ProteinModelPortaliQ8CD91.
SMRiQ8CD91.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024660.

Proteomic databases

PaxDbiQ8CD91.
PRIDEiQ8CD91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024660; ENSMUSP00000024660; ENSMUSG00000023886. [Q8CD91-1]
GeneIDi64074.
KEGGimmu:64074.
UCSCiuc008amy.2. mouse. [Q8CD91-1]
uc008ana.2. mouse. [Q8CD91-2]

Organism-specific databases

CTDi64094.
MGIiMGI:1929881. Smoc2.

Phylogenomic databases

eggNOGiKOG4578. Eukaryota.
ENOG410YP7C. LUCA.
GeneTreeiENSGT00390000018436.
HOGENOMiHOG000234328.
HOVERGENiHBG058558.
InParanoidiQ8CD91.
OMAiPRCPGSI.
OrthoDBiEOG091G083O.
PhylomeDBiQ8CD91.
TreeFamiTF320666.

Miscellaneous databases

PROiQ8CD91.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023886.
CleanExiMM_SMOC2.
GenevisibleiQ8CD91. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMOC2_MOUSE
AccessioniPrimary (citable) accession number: Q8CD91
Secondary accession number(s): Q8VCM1, Q9D9K2, Q9ER95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.