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Protein

SPARC-related modular calcium-binding protein 2

Gene

Smoc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can stimulate endothelial cell proliferation, migration, as well as angiogenesis (By similarity). Promotes matrix assembly and cell adhesiveness.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi360 – 371121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi397 – 408122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • glycosaminoglycan binding Source: MGI
  • heparin binding Source: MGI

GO - Biological processi

  • extracellular matrix organization Source: MGI
  • positive regulation of cell-substrate adhesion Source: MGI
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC-related modular calcium-binding protein 2
Alternative name(s):
Secreted modular calcium-binding protein 2
Short name:
SMOC-2
Gene namesi
Name:Smoc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1929881. Smoc2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • interstitial matrix Source: MGI
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 447426SPARC-related modular calcium-binding protein 2PRO_0000020319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi90 ↔ 113By similarity
Disulfide bondi124 ↔ 131By similarity
Disulfide bondi133 ↔ 153By similarity
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence analysis
Disulfide bondi216 ↔ 240By similarity
Disulfide bondi251 ↔ 258By similarity
Disulfide bondi260 ↔ 281By similarity
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8CD91.
PaxDbiQ8CD91.
PRIDEiQ8CD91.

Expressioni

Tissue specificityi

Strongly expressed in ovary, followed by heart, muscle, spleen, brain, thymus, lung, liver, kidney, spleen, testis, ovary and skeletal muscle.1 Publication

Developmental stagei

At E16.5, present in rib cartilage, choroid plexus epithelium and associated blood vessels, and developing oral and tooth germ epithelia (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000023886.
CleanExiMM_SMOC2.
GenevisibleiQ8CD91. MM.

Interactioni

Subunit structurei

Binds various proteins from the extracellular matrix.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024660.

Structurei

3D structure databases

ProteinModelPortaliQ8CD91.
SMRiQ8CD91. Positions 352-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 8653Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini87 – 15367Thyroglobulin type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini213 – 28169Thyroglobulin type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini347 – 38236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini384 – 41936EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 2 thyroglobulin type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4578. Eukaryota.
ENOG410YP7C. LUCA.
GeneTreeiENSGT00390000018436.
HOGENOMiHOG000234328.
HOVERGENiHBG058558.
InParanoidiQ8CD91.
OMAiPRCPGSI.
OrthoDBiEOG091G083O.
PhylomeDBiQ8CD91.
TreeFamiTF320666.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CD91-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPPQLCWLP LLAALLPPVP AQKFSALTFL RVDQDKDRDC SLDCPSSPQK
60 70 80 90 100
PLCASDGRTF LSRCEFQRAK CKDPQLEIAH RGNCKDVSRC VAERKYTQEQ
110 120 130 140 150
ARKEFQQVFI PECNDDGTYS QVQCHSYTGY CWCVTPNGRP ISGTAVAHKT
160 170 180 190 200
PRCPGSINEK VPQREGAGKA DDAAAPALET QPQGDEEDIA SRYPTLWTEQ
210 220 230 240 250
VKSRQNKTNK NSASSCDQEH QSALEEAKQP KNDNVVIPEC AHGGLYKPVQ
260 270 280 290 300
CHPSTGYCWC VLVDTGRPIP GTSTRYEQPK CDNTARAHPA KARDLYKNRP
310 320 330 340 350
LQGCPGAKKH EFLTSVLDAL STDMVHAVSD PSSSSGRLSE PDPSHTLEER
360 370 380 390 400
VVHWYFKLLD KNSSGDIGKK EIKPFKRFLR KKSKPKKCVK KFVEYCDMNN
410 420 430 440
DKSITVQELM GCLGVTREEG KANTRKRHTP RGNAESSSSN RQPRKQG
Length:447
Mass (Da):49,891
Last modified:March 1, 2003 - v1
Checksum:i26AB802F9D13A00C
GO
Isoform 2 (identifier: Q8CD91-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-336: MLPPQLCWLP...AVSDPSSSSG → MLLFLDLSPGL

Note: No experimental confirmation available.
Show »
Length:122
Mass (Da):14,077
Checksum:iCBD913F9F984CF06
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti351 – 3511V → D in BAB24753 (PubMed:16141072).Curated
Sequence conflicti437 – 4382SS → T (PubMed:12741954).Curated
Sequence conflicti439 – 4391S → F in BAB24753 (PubMed:16141072).Curated
Sequence conflicti440 – 4401N → Y in AAH19527 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 336336MLPPQ…SSSSG → MLLFLDLSPGL in isoform 2. 1 PublicationVSP_008723Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030958 mRNA. Translation: BAC27193.1.
AK006809 mRNA. Translation: BAB24753.1.
AJ249901 mRNA. Translation: CAC10355.1.
BC019527 mRNA. Translation: AAH19527.1.
CCDSiCCDS37442.1. [Q8CD91-1]
RefSeqiNP_071710.2. NM_022315.2. [Q8CD91-1]
UniGeneiMm.30162.

Genome annotation databases

EnsembliENSMUST00000024660; ENSMUSP00000024660; ENSMUSG00000023886. [Q8CD91-1]
GeneIDi64074.
KEGGimmu:64074.
UCSCiuc008amy.2. mouse. [Q8CD91-1]
uc008ana.2. mouse. [Q8CD91-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030958 mRNA. Translation: BAC27193.1.
AK006809 mRNA. Translation: BAB24753.1.
AJ249901 mRNA. Translation: CAC10355.1.
BC019527 mRNA. Translation: AAH19527.1.
CCDSiCCDS37442.1. [Q8CD91-1]
RefSeqiNP_071710.2. NM_022315.2. [Q8CD91-1]
UniGeneiMm.30162.

3D structure databases

ProteinModelPortaliQ8CD91.
SMRiQ8CD91. Positions 352-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024660.

Proteomic databases

MaxQBiQ8CD91.
PaxDbiQ8CD91.
PRIDEiQ8CD91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024660; ENSMUSP00000024660; ENSMUSG00000023886. [Q8CD91-1]
GeneIDi64074.
KEGGimmu:64074.
UCSCiuc008amy.2. mouse. [Q8CD91-1]
uc008ana.2. mouse. [Q8CD91-2]

Organism-specific databases

CTDi64094.
MGIiMGI:1929881. Smoc2.

Phylogenomic databases

eggNOGiKOG4578. Eukaryota.
ENOG410YP7C. LUCA.
GeneTreeiENSGT00390000018436.
HOGENOMiHOG000234328.
HOVERGENiHBG058558.
InParanoidiQ8CD91.
OMAiPRCPGSI.
OrthoDBiEOG091G083O.
PhylomeDBiQ8CD91.
TreeFamiTF320666.

Miscellaneous databases

PROiQ8CD91.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023886.
CleanExiMM_SMOC2.
GenevisibleiQ8CD91. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 2 hits.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMOC2_MOUSE
AccessioniPrimary (citable) accession number: Q8CD91
Secondary accession number(s): Q8VCM1, Q9D9K2, Q9ER95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.