Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA

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Q8CD15 (MINA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA
EC=1.14.11.-

Alternative name(s):
Histone lysine demethylase MINA
MYC-induced nuclear antigen

Gene names

Name:	Mina
Synonyms:	Mina53

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39' By similarity. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. Ref.1
Catalytic activity	L-histidine-[60S ribosomal protein L27a] + 2-oxoglutarate + O₂ = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L27a] + succinate + CO₂.
Cofactor	Binds 1 Fe²⁺ ion per subunit By similarity.
Subcellular location	Nucleus. Nucleus › nucleolus Ref.1.
Tissue specificity	Predominantly expressed in testis. Expressed at high levels in spleen, thymus, and colon, but barely detectable in brain, skeletal muscle, and seminal vesicle (at protein level). Ref.1
Developmental stage	In testis, expressed in the nuclei of spermatogonia at all stages of the seminiferous epithelial cycle, and in meiotic prophase cells such as preleptotene, leptotene and zygotene, and weakly in early pachytene spermatocytes, but is absent in late pachytene spermatocytes, spermatids and mature sperm (at protein level). Ref.1
Induction	Up-regulated in experimentally-induced cryptorchid testis. Ref.1
Sequence similarities	Belongs to the MINA53/NO66 family. Contains 1 JmjC domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell proliferation Inferred from sequence orthology PubMed 12091391. Source: MGI ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 19561615. Source: MGI nucleolus Inferred from sequence orthology PubMed 12091391. Source: MGI transcription factor complex Inferred from physical interaction PubMed 19561615. Source: MGI
Molecular_function	RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Inferred from genetic interaction PubMed 19561615. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 465

465

Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA

PRO_0000308378

Regions

Domain

139 – 271

133

JmjC

Sites

Metal binding

179

Iron; catalytic By similarity

Metal binding

181

Iron; catalytic By similarity

Metal binding

240

Iron; catalytic By similarity

Amino acid modifications

Modified residue

309

Phosphoserine By similarity

Experimental info

Sequence conflict

A → E in AAH25109. Ref.3

Sequence conflict

A → E in AAH23462. Ref.3

Sequence conflict

107

S → N in BAC27503. Ref.2

Sequence conflict

440

D → E in AAH25109. Ref.3

Sequence conflict

440

D → E in AAH23462. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CD15 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: E0504FE459FFC47A
Show »

FASTA

465

53,517

        10         20         30         40         50         60 
MPKKVQPTGD ENEEASVPCK RVKEELPETL SVLNFDSPSS FFESLISPIK VETFFKEFWE 

        70         80         90        100        110        120 
QKPLLIQRDD PVLAKYYQSL FSLSDLKRLC KKGVYYGRDV NVCRSISGKK KVLNKDGRAH 

       130        140        150        160        170        180 
FLQLRKDFDQ KRATIQFHQP QRYKDELWRI QEKLECYFGS LVGSNVYMTP AGSQGLPPHY 

       190        200        210        220        230        240 
DDVEVFILQL EGTKHWRLYS PTVPLAHEYS VESEDRIGTP THDFLLKPGD LLYFPRGTIH 

       250        260        270        280        290        300 
QAETPSGLAY SIHLTISTYQ NNSWGDCLLD SISGFVFDIA KEDVALRSGM PRRMLLNVET 

       310        320        330        340        350        360 
PADVTRKLSG FLRTLADQLE GREELLSSDM KKDFVKHRLP PFFEGNGTET MDPGKQLPRL 

       370        380        390        400        410        420 
DNIIRLQFKD HIVLTVGPDK NPFDEAQQKV VYIYHSLKNV RQMHMIGEEE ESEIFGLRFP 

       430        440        450        460 
LSHVDALKQI WCGSPIRVKD LKLDTDEEKE NLALSLWSES LIQVL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Mina53, a product of a Myc target gene in mouse testis." Tsuneoka M., Nishimune Y., Ohta K., Teye K., Tanaka H., Soejima M., Iida H., Inokuchi T., Kimura H., Koda Y. Int. J. Androl. 29:323-330(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Tissue: Stomach.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo, Embryonic testis and Pancreas.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Kidney and Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB177385 mRNA. Translation: BAD60965.1. AK007810 mRNA. Translation: BAB25275.1. AK013451 mRNA. Translation: BAB28860.1. AK031671 mRNA. Translation: BAC27503.1. BC023462 mRNA. Translation: AAH23462.1. BC025109 mRNA. Translation: AAH25109.1. BC058242 mRNA. Translation: AAH58242.1.
IPI	IPI00131778.
RefSeq	NP_080186.3. NM_025910.3.
UniGene	Mm.289150.
3D structure databases
ProteinModelPortal	Q8CD15.
SMR	Q8CD15. Positions 31-465.
ModBase	Search...
PTM databases
PhosphoSite	Q8CD15.
Proteomic databases
PaxDb	Q8CD15.
PRIDE	Q8CD15.
Protocols and materials databases
DNASU	67014.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023407; ENSMUSP00000023407; ENSMUSG00000022724. ENSMUST00000160571; ENSMUSP00000125297; ENSMUSG00000022724.
GeneID	67014.
KEGG	mmu:67014.
UCSC	uc007zph.2. mouse.
Organism-specific databases
CTD	84864.
MGI	MGI:1914264. Mina.
Phylogenomic databases
eggNOG	NOG83808.
GeneTree	ENSGT00390000000083.
HOGENOM	HOG000007965.
HOVERGEN	HBG055265.
InParanoid	Q8CD15.
OMA	STYQNNS.
OrthoDB	EOG4W6NVT.
Gene expression databases
ArrayExpress	Q8CD15.
Bgee	Q8CD15.
CleanEx	MM_MINA.
Genevestigator	Q8CD15.
Family and domain databases
InterPro	IPR003347. JmjC_dom. IPR013109. NO66/MINA. [Graphical view]
PANTHER	PTHR13096. PTHR13096. 1 hit.
Pfam	PF08007. Cupin_4. 1 hit. [Graphical view]
SMART	SM00558. JmjC. 1 hit. [Graphical view]
PROSITE	PS51184. JMJC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MINA. mouse.
NextBio	323310.
SOURCE	Search...

Entry information

Entry name

MINA_MOUSE

Accession

Primary (citable) accession number: Q8CD15
Secondary accession number(s): Q8QZX1, Q9CQ03

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents