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Protein

RING1 and YY1-binding protein

Gene

Rybp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 50RanBP2-typePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

  • DNA binding Source: MGI
  • transcription corepressor activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RING1 and YY1-binding protein
Alternative name(s):
Death effector domain-associated factor
Short name:
DED-associated factor
Gene namesi
Name:Rybp
Synonyms:Dedaf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1929059. Rybp.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • PcG protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonically lethal. Embryos die at early postimplantation stage.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31 – 32TF → AA: Loss of ubiquitin binding. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975511 – 228RING1 and YY1-binding proteinAdd BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99PhosphoserineBy similarity1
Modified residuei123PhosphoserineCombined sources1
Modified residuei127PhosphoserineCombined sources1
Modified residuei130PhosphoserineCombined sources1
Modified residuei227PhosphoserineBy similarity1

Post-translational modificationi

Monoubiquitinated.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CCI5.
MaxQBiQ8CCI5.
PaxDbiQ8CCI5.
PeptideAtlasiQ8CCI5.
PRIDEiQ8CCI5.

PTM databases

iPTMnetiQ8CCI5.
PhosphoSitePlusiQ8CCI5.

Expressioni

Developmental stagei

At day E9.0, selectively expressed in cells of the developing nervous system and from day E.5 onwards, expressed ubiquitously.1 Publication

Gene expression databases

BgeeiENSMUSG00000072872.
CleanExiMM_RYBP.
GenevisibleiQ8CCI5. MM.

Interactioni

Subunit structurei

Monomer. Interacts with apoptin, DEDD, FADD, CASP8, CASP10, YY1 and GABPB1. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of BCOR, PCGF1, RING1 and RNF2/RING2. Interacts with MDM2, and thereby inhibits ubiquitination of TP53. Identified in a ternary complex containing MDM2, TP53 and RYBP (By similarity). Together with GABPB1 and YY1, it forms a ternary complex, probably being the bridge factor between these two transcription factors. Interacts with CBX2, YAF2, RING1 and RNF2. Interacts with ubiquitin and ubiquitinated proteins. Interacts with ubiquitinated histone H2A. Interacts with PCGF1 (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cbx2P306583EBI-929290,EBI-360174
Ring1O357304EBI-929290,EBI-929310
Rnf2Q9CQJ48EBI-929290,EBI-927321
YY1P254902EBI-929290,EBI-765538From a different organism.

Protein-protein interaction databases

BioGridi207918. 13 interactors.
IntActiQ8CCI5. 14 interactors.
MINTiMINT-4115157.
STRINGi10090.ENSMUSP00000098677.

Structurei

3D structure databases

ProteinModelPortaliQ8CCI5.
SMRiQ8CCI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni143 – 226Interaction with E4TF1BBy similarityAdd BLAST84

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi73 – 76Poly-Pro4
Compositional biasi77 – 119Lys-richAdd BLAST43
Compositional biasi179 – 214Ser-richAdd BLAST36

Domaini

Intrinsically unstructured in the absence of binding partners. Folds upon binding to DNA or RNF2.1 Publication

Sequence similaritiesi

Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 50RanBP2-typePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4477. Eukaryota.
ENOG4111PN1. LUCA.
GeneTreeiENSGT00390000013995.
HOGENOMiHOG000007553.
HOVERGENiHBG001768.
InParanoidiQ8CCI5.
KOiK11469.
OMAiSHISRPK.
OrthoDBiEOG091G10HB.
PhylomeDBiQ8CCI5.
TreeFamiTF350501.

Family and domain databases

InterProiIPR033774. YAF2_RYBP.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF17219. YAF2_RYBP. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CCI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMGDKKSPT RPKRQAKPAA DEGFWDCSVC TFRNSAEAFK CSICDVRKGT
60 70 80 90 100
STRKPRINSQ LVAQQVAQQY ATPPPPKKEK KEKVEKPDKE KPEKDKDISP
110 120 130 140 150
SVTKKNTNKK TKPKSDILKD PPSEANSIQS ANATTKTSET NHTSRPRLKN
160 170 180 190 200
VDRSTAQQLA VTVGNVTVII TDFKEKTRSS STSSSTVTSS AGSEQQNQSS
210 220
SGSESTDKGS SRSSTPKGDM SAVNDESF
Length:228
Mass (Da):24,777
Last modified:March 1, 2003 - v1
Checksum:i80E4D647244E213D
GO

Sequence cautioni

The sequence AAD42945 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143T → S in AAD42945 (PubMed:10369680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033024 mRNA. Translation: BAC28131.1.
BC053016 mRNA. Translation: AAH53016.1.
BC080287 mRNA. Translation: AAH80287.1.
BC138324 mRNA. Translation: AAI38325.1.
BC138325 mRNA. Translation: AAI38326.1.
AF101779 mRNA. Translation: AAD42945.1. Different initiation.
CCDSiCCDS39579.1.
RefSeqiNP_062717.2. NM_019743.3.
UniGeneiMm.321633.

Genome annotation databases

EnsembliENSMUST00000101118; ENSMUSP00000098677; ENSMUSG00000072872.
GeneIDi56353.
KEGGimmu:56353.
UCSCiuc009dbw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033024 mRNA. Translation: BAC28131.1.
BC053016 mRNA. Translation: AAH53016.1.
BC080287 mRNA. Translation: AAH80287.1.
BC138324 mRNA. Translation: AAI38325.1.
BC138325 mRNA. Translation: AAI38326.1.
AF101779 mRNA. Translation: AAD42945.1. Different initiation.
CCDSiCCDS39579.1.
RefSeqiNP_062717.2. NM_019743.3.
UniGeneiMm.321633.

3D structure databases

ProteinModelPortaliQ8CCI5.
SMRiQ8CCI5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207918. 13 interactors.
IntActiQ8CCI5. 14 interactors.
MINTiMINT-4115157.
STRINGi10090.ENSMUSP00000098677.

PTM databases

iPTMnetiQ8CCI5.
PhosphoSitePlusiQ8CCI5.

Proteomic databases

EPDiQ8CCI5.
MaxQBiQ8CCI5.
PaxDbiQ8CCI5.
PeptideAtlasiQ8CCI5.
PRIDEiQ8CCI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101118; ENSMUSP00000098677; ENSMUSG00000072872.
GeneIDi56353.
KEGGimmu:56353.
UCSCiuc009dbw.1. mouse.

Organism-specific databases

CTDi23429.
MGIiMGI:1929059. Rybp.

Phylogenomic databases

eggNOGiKOG4477. Eukaryota.
ENOG4111PN1. LUCA.
GeneTreeiENSGT00390000013995.
HOGENOMiHOG000007553.
HOVERGENiHBG001768.
InParanoidiQ8CCI5.
KOiK11469.
OMAiSHISRPK.
OrthoDBiEOG091G10HB.
PhylomeDBiQ8CCI5.
TreeFamiTF350501.

Miscellaneous databases

ChiTaRSiRybp. mouse.
PROiQ8CCI5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072872.
CleanExiMM_RYBP.
GenevisibleiQ8CCI5. MM.

Family and domain databases

InterProiIPR033774. YAF2_RYBP.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF17219. YAF2_RYBP. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRYBP_MOUSE
AccessioniPrimary (citable) accession number: Q8CCI5
Secondary accession number(s): B2RRB6, Q9WVK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.