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Protein

U4/U6 small nuclear ribonucleoprotein Prp31

Gene

Prpf31

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein Prp31
Alternative name(s):
Pre-mRNA-processing factor 31
U4/U6 snRNP 61 kDa protein
Short name:
Protein 61K
Gene namesi
Name:Prpf31
Synonyms:Prp31
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1916238. Prpf31.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002278001 – 499U4/U6 small nuclear ribonucleoprotein Prp31Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei379PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei438N6-acetyllysineCombined sources1
Modified residuei439PhosphoserineBy similarity1
Modified residuei440PhosphothreonineBy similarity1
Modified residuei450PhosphoserineBy similarity1
Modified residuei455PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CCF0.
PeptideAtlasiQ8CCF0.
PRIDEiQ8CCF0.

PTM databases

iPTMnetiQ8CCF0.
PhosphoSitePlusiQ8CCF0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000008373.
GenevisibleiQ8CCF0. MM.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Interacts with a complex formed by SNU13 and U4 snRNA, but not with SNU13 or U4 snRNA alone. Interacts with PRPF6/U5 snRNP-associated 102 kDa protein. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts (via its NLS) with CTNNBL1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei247Interaction with U4 snRNABy similarity1
Sitei270Interaction with U4 snRNABy similarity1

Protein-protein interaction databases

BioGridi213160. 2 interactors.
IntActiQ8CCF0. 2 interactors.
MINTiMINT-4115085.
STRINGi10090.ENSMUSP00000008517.

Structurei

3D structure databases

ProteinModelPortaliQ8CCF0.
SMRiQ8CCF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 333NopPROSITE-ProRule annotationAdd BLAST119

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 120By similarityAdd BLAST36
Coiled coili181 – 215By similarityAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi351 – 364Nuclear localization signal (NLS)By similarityAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi16 – 19Poly-Glu4
Compositional biasi25 – 29Poly-Glu5

Domaini

Interacts with the snRNP via the Nop domain.By similarity
The coiled coil domain is formed by two non-contiguous helices.By similarity

Sequence similaritiesi

Belongs to the PRP31 family.Curated
Contains 1 Nop domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2574. Eukaryota.
COG1498. LUCA.
GeneTreeiENSGT00550000075069.
HOVERGENiHBG082193.
InParanoidiQ8CCF0.
KOiK12844.
OMAiCDMAVEL.
OrthoDBiEOG091G09U7.
TreeFamiTF300677.

Family and domain databases

InterProiIPR002687. Nop_dom.
IPR012976. NOSIC.
IPR027105. Prp31.
IPR019175. Prp31_C.
[Graphical view]
PANTHERiPTHR13904. PTHR13904. 1 hit.
PfamiPF01798. Nop. 1 hit.
PF09785. Prp31_C. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CCF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKS
60 70 80 90 100
IAKLWDSKMF AEIMMKIEEY ISKQANVSEV MGPVEAAPEY RVIVDANNLT
110 120 130 140 150
VEIENELNII HKFIRDKYSK RFPELESLVP NALDYIRTVK ELGNSLDKCK
160 170 180 190 200
NNENLQQILT NATIMVVSVT ASTTQGQQLS DEELERLEEA CDMALELNAS
210 220 230 240 250
KHRIYEYVES RMSFIAPNLS IIIGASTAAK IMGVAGGLTN LSKMPACNIM
260 270 280 290 300
LLGAQRKTLS GFSSTSVLPH TGYIYHSDIV QSLPPDLRRK AARLVAAKCT
310 320 330 340 350
LAARVDSFHE STEGKVGYEL KDEIERKFDK WQEPPPVKQV KPLPAPLDGQ
360 370 380 390 400
RKKRGGRRYR KMKERLGLTE IRKQANRMSF GEIEEDAYQE DLGFSLGHLG
410 420 430 440 450
KSGSGRVRQT QVNEATKARI SKTLQRTLQK QSVVYGGKST IRDRSSGTAS
460 470 480 490
SVAFTPLQGL EIVNPQAAEK KVAEANQKYF SSMAEFLKVK GEKSGTMST
Length:499
Mass (Da):55,430
Last modified:July 27, 2011 - v3
Checksum:iA8149257A6213D4F
GO
Isoform 2 (identifier: Q8CCF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-321: Missing.

Note: No experimental confirmation available.
Show »
Length:493
Mass (Da):54,740
Checksum:i4D95CBC24749FA1A
GO
Isoform 4 (identifier: Q8CCF0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-65: FAEIMM → VSLLRS
     66-499: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:65
Mass (Da):7,146
Checksum:iFE35F67E54C4EF98
GO

Sequence cautioni

The sequence BAC25109 differs from that shown. Reason: Frameshift at position 357.Curated
The sequence BAC31903 differs from that shown. Contaminating sequence. The C-terminus matches chromosome 19 region.Curated
The sequence BAC31931 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated
The sequence BAC34578 differs from that shown. Reason: Frameshift at position 7.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77V → A in AAK77987 (PubMed:11867543).Curated1
Sequence conflicti77V → A in BAC31931 (PubMed:16141072).Curated1
Sequence conflicti77V → A in CAJ18397 (Ref. 3) Curated1
Sequence conflicti77V → A in AAH18376 (PubMed:15489334).Curated1
Sequence conflicti77V → A in AAH57877 (PubMed:15489334).Curated1
Sequence conflicti104E → V in BAC28192 (PubMed:16141072).Curated1
Sequence conflicti177Q → R in BAC28220 (PubMed:16141072).Curated1
Sequence conflicti177Q → R in BAC28192 (PubMed:16141072).Curated1
Sequence conflicti382E → G in BAC25109 (PubMed:16141072).Curated1
Sequence conflicti481S → F in AAH61461 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01758960 – 65FAEIMM → VSLLRS in isoform 4. 1 Publication6
Alternative sequenceiVSP_01759066 – 499Missing in isoform 4. 1 PublicationAdd BLAST434
Alternative sequenceiVSP_017591316 – 321Missing in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040823 mRNA. Translation: AAK77987.1.
AK005294 mRNA. Translation: BAC25109.1. Frameshift.
AK033190 mRNA. Translation: BAC28192.1.
AK033283 mRNA. Translation: BAC28220.1.
AK044398 mRNA. Translation: BAC31903.1. Sequence problems.
AK044457 mRNA. Translation: BAC31931.1. Sequence problems.
AK051260 mRNA. Translation: BAC34578.1. Frameshift.
CT010189 mRNA. Translation: CAJ18397.1.
AC130680 Genomic DNA. No translation available.
BC018376 mRNA. Translation: AAH18376.1.
BC057877 mRNA. Translation: AAH57877.1.
BC061461 mRNA. Translation: AAH61461.1.
CCDSiCCDS39729.1. [Q8CCF0-1]
CCDS51965.1. [Q8CCF0-2]
RefSeqiNP_001153186.1. NM_001159714.1. [Q8CCF0-2]
NP_081604.3. NM_027328.4. [Q8CCF0-1]
UniGeneiMm.246863.

Genome annotation databases

EnsembliENSMUST00000008517; ENSMUSP00000008517; ENSMUSG00000008373. [Q8CCF0-1]
ENSMUST00000108636; ENSMUSP00000104276; ENSMUSG00000008373. [Q8CCF0-2]
ENSMUST00000125782; ENSMUSP00000146017; ENSMUSG00000008373. [Q8CCF0-4]
ENSMUST00000179769; ENSMUSP00000136031; ENSMUSG00000008373. [Q8CCF0-2]
GeneIDi68988.
KEGGimmu:68988.
UCSCiuc009evi.2. mouse. [Q8CCF0-1]
uc012ewf.1. mouse. [Q8CCF0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040823 mRNA. Translation: AAK77987.1.
AK005294 mRNA. Translation: BAC25109.1. Frameshift.
AK033190 mRNA. Translation: BAC28192.1.
AK033283 mRNA. Translation: BAC28220.1.
AK044398 mRNA. Translation: BAC31903.1. Sequence problems.
AK044457 mRNA. Translation: BAC31931.1. Sequence problems.
AK051260 mRNA. Translation: BAC34578.1. Frameshift.
CT010189 mRNA. Translation: CAJ18397.1.
AC130680 Genomic DNA. No translation available.
BC018376 mRNA. Translation: AAH18376.1.
BC057877 mRNA. Translation: AAH57877.1.
BC061461 mRNA. Translation: AAH61461.1.
CCDSiCCDS39729.1. [Q8CCF0-1]
CCDS51965.1. [Q8CCF0-2]
RefSeqiNP_001153186.1. NM_001159714.1. [Q8CCF0-2]
NP_081604.3. NM_027328.4. [Q8CCF0-1]
UniGeneiMm.246863.

3D structure databases

ProteinModelPortaliQ8CCF0.
SMRiQ8CCF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213160. 2 interactors.
IntActiQ8CCF0. 2 interactors.
MINTiMINT-4115085.
STRINGi10090.ENSMUSP00000008517.

PTM databases

iPTMnetiQ8CCF0.
PhosphoSitePlusiQ8CCF0.

Proteomic databases

PaxDbiQ8CCF0.
PeptideAtlasiQ8CCF0.
PRIDEiQ8CCF0.

Protocols and materials databases

DNASUi68988.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008517; ENSMUSP00000008517; ENSMUSG00000008373. [Q8CCF0-1]
ENSMUST00000108636; ENSMUSP00000104276; ENSMUSG00000008373. [Q8CCF0-2]
ENSMUST00000125782; ENSMUSP00000146017; ENSMUSG00000008373. [Q8CCF0-4]
ENSMUST00000179769; ENSMUSP00000136031; ENSMUSG00000008373. [Q8CCF0-2]
GeneIDi68988.
KEGGimmu:68988.
UCSCiuc009evi.2. mouse. [Q8CCF0-1]
uc012ewf.1. mouse. [Q8CCF0-2]

Organism-specific databases

CTDi26121.
MGIiMGI:1916238. Prpf31.

Phylogenomic databases

eggNOGiKOG2574. Eukaryota.
COG1498. LUCA.
GeneTreeiENSGT00550000075069.
HOVERGENiHBG082193.
InParanoidiQ8CCF0.
KOiK12844.
OMAiCDMAVEL.
OrthoDBiEOG091G09U7.
TreeFamiTF300677.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ8CCF0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008373.
GenevisibleiQ8CCF0. MM.

Family and domain databases

InterProiIPR002687. Nop_dom.
IPR012976. NOSIC.
IPR027105. Prp31.
IPR019175. Prp31_C.
[Graphical view]
PANTHERiPTHR13904. PTHR13904. 1 hit.
PfamiPF01798. Nop. 1 hit.
PF09785. Prp31_C. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP31_MOUSE
AccessioniPrimary (citable) accession number: Q8CCF0
Secondary accession number(s): E9QPM6
, Q6P7X2, Q8BQ91, Q8C8U4, Q8C8V5, Q8CCG6, Q8CF52, Q8VBW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.