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Protein

Synaptopodin

Gene

Synpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI

GO - Biological processi

  • cortical cytoskeleton organization Source: UniProtKB
  • positive regulation of actin filament bundle assembly Source: UniProtKB
  • regulation of long-term neuronal synaptic plasticity Source: GOC-OWL
  • regulation of stress fiber assembly Source: UniProtKB
  • spine apparatus assembly Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptopodin
Gene namesi
Name:Synpo
Synonyms:Kiaa1029
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1099446. Synpo.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • bicellular tight junction Source: UniProtKB-SubCell
  • dendrite Source: MGI
  • dendritic spine Source: UniProtKB
  • nucleus Source: GO_Central
  • perikaryon Source: UniProtKB-SubCell
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
  • stress fiber Source: UniProtKB
  • Z disc Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001876711 – 929SynaptopodinAdd BLAST929

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei134PhosphoserineCombined sources1
Modified residuei202PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei512PhosphoserineBy similarity1
Modified residuei547PhosphothreonineBy similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei672PhosphoserineCombined sources1
Modified residuei689PhosphoserineCombined sources1
Modified residuei740PhosphoserineCombined sources1
Modified residuei744PhosphoserineCombined sources1
Modified residuei765PhosphoserineBy similarity1
Modified residuei769PhosphothreonineCombined sources1
Modified residuei833PhosphoserineCombined sources1
Modified residuei848Omega-N-methylarginineCombined sources1
Modified residuei854PhosphoserineBy similarity1
Isoform 2 (identifier: Q8CC35-2)
Modified residuei690PhosphoserineCombined sourcesBy similarity1
Modified residuei786Omega-N-methylarginineCombined sourcesBy similarity1
Modified residuei789PhosphoserineCombined sourcesBy similarity1
Modified residuei800Omega-N-methylarginineCombined sourcesBy similarity1
Modified residuei803PhosphoserineCombined sourcesBy similarity1
Modified residuei814Omega-N-methylarginineCombined sourcesBy similarity1
Modified residuei819PhosphoserineCombined sourcesBy similarity1
Modified residuei824PhosphoserineCombined sourcesBy similarity1
Modified residuei852PhosphoserineCombined sourcesBy similarity1
Modified residuei869Phosphoserine By similarityCombined sourcesBy similarity1
Modified residuei892PhosphoserineCombined sourcesBy similarity1

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CC35.
PeptideAtlasiQ8CC35.
PRIDEiQ8CC35.

PTM databases

iPTMnetiQ8CC35.
PhosphoSitePlusiQ8CC35.

Expressioni

Tissue specificityi

Expressed in brain, namely in the olfactory bulb, cerebral cortex, striatum, and hippocampus, but not in the cerebellum. Also expressed in the podocytes of kidney glomeruli. In the hippocampus, mainly expressed in the principal cell layer of the dentate gyrus and Ammon's horn.3 Publications

Gene expression databases

BgeeiENSMUSG00000043079.
CleanExiMM_SYNPO.
ExpressionAtlasiQ8CC35. baseline and differential.
GenevisibleiQ8CC35. MM.

Interactioni

Subunit structurei

Interacts with BAIAP1. Interacts with actin. Interacts (via PPxY motifs) with WWC1 (via WW domains) (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: MGI

Protein-protein interaction databases

IntActiQ8CC35. 2 interactors.
STRINGi10090.ENSMUSP00000095174.

Structurei

3D structure databases

ProteinModelPortaliQ8CC35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi549 – 552PPxY motif4
Motifi568 – 571PPxY motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi766 – 929Pro-richAdd BLAST164

Sequence similaritiesi

Belongs to the synaptopodin family.Curated

Phylogenomic databases

eggNOGiENOG410IHBK. Eukaryota.
ENOG4111RGH. LUCA.
GeneTreeiENSGT00530000063754.
HOGENOMiHOG000132998.
HOVERGENiHBG056954.
InParanoidiQ8CC35.
PhylomeDBiQ8CC35.
TreeFamiTF330867.

Family and domain databases

InterProiIPR028753. Synpo.
[Graphical view]
PANTHERiPTHR24217:SF13. PTHR24217:SF13. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CC35-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGAHFPPPP LGASEGRAAP CTFQIPDGSY RCLALEAEES SSEDGLQGEV
60 70 80 90 100
RLVDLEEEGT SQSRANHGTP PLSRAPAIIQ PSSCHREARG GFQRSDRPSH
110 120 130 140 150
DWDVVQARKV MTASGSSSPV PRVAQKPALG RSTSFTENDL KEAKARSQQI
160 170 180 190 200
AAQLTTPPSS NSRGVQLFNR RRQRVNEFTL ESRGQRSPKL NQEALQTGRP
210 220 230 240 250
LSPIGHAPGP SVKPTSPSKP GSPKHPSPQS PSRGVAGHIM EGYSEEASLL
260 270 280 290 300
RHLEKVASEE EEVPLVVYLK ENAALLTANG LHLSQSRETQ QSSPNPPDTE
310 320 330 340 350
VPSPAADINQ NPSSPNATLT TLASSSHHSQ PTADINQNPP AAITPVPQNS
360 370 380 390 400
SQAQCSPNGT LDSKPGTLCA DDGQSPVPAE EVRSSILLID KVSAPPSAAS
410 420 430 440 450
TFSREATPLS SSGPPAADLM SSSLLIDMQP STLVAPAEQE VPGHVAVTTP
460 470 480 490 500
TKVYSEVHLT LAKPASVVNR TARPFGIQSP GTSQIEQSPM MGRRQFGEKA
510 520 530 540 550
WAPPASSMAD RSPQPQRHIM SRSPMVERRL LGQRSPVLER RPLGNFTPPP
560 570 580 590 600
TYAETLSTAP VASRVRSPPS YSTLYPSSDP KPSHLKGQVA PANKTGILEE
610 620 630 640 650
SMARRGSRKS MFTFVEKPKV TPNPDLLDLV QTADEKRRQR DHGEVGMEEE
660 670 680 690 700
PFALGAEASN FQQEPIARDR ASPAAAEEAV PEWASCLKSP RIQAKPKPKP
710 720 730 740 750
NQNLSEASGK GAELYARRQS RMEKYVIESS GHAELARCPS PTMSLPSSWK
760 770 780 790 800
YTTNAPGGFR VASLSPARTP PASLYHGYLP ENGVLRPEPT KQQPYQMRPS
810 820 830 840 850
LYALSPVKEP AKASSRATSS RTPSRTVSPR AASPAKPSSL DLVPNLPRAG
860 870 880 890 900
LPPSPALPRP SRSSPGLYTA PVQDSLQPTA VSPTYSSDIS PVSPSRAWSP
910 920
RAKQAPRPSF STRNAGIEAQ VWKPSFCFK
Note: No experimental confirmation available.
Length:929
Mass (Da):99,552
Last modified:June 7, 2004 - v2
Checksum:i979CFEE4F9E061A2
GO
Isoform 2 (identifier: Q8CC35-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-239: Missing.
     921-929: VWKPSFCFK → DRPESLPTSP...SLPTEASCTT

Show »
Length:901
Mass (Da):96,250
Checksum:i14EC69FE4E4889D6
GO
Isoform 3 (identifier: Q8CC35-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-239: Missing.

Show »
Length:690
Mass (Da):74,017
Checksum:i548D75B2AF5BBB62
GO

Sequence cautioni

The sequence BAC28546 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC98077 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189K → M (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0104781 – 239Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST239
Alternative sequenceiVSP_010479921 – 929VWKPSFCFK → DRPESLPTSPPWTPAASRPP SSLDGWVSPGPWEPGRGSSM SSPPPLPPPPPMSPSWSERS VSPLRSETEARPPSRQLQAL LARNIINAARRKSASPRPAP AETLRPFSPPQGPPPPPARM RSPQPASPARNFRGAAFSPI PRSPLPIGPSSCASPRSPQA APSRPFPYRRSPTDSDVSLD SEDSGLKSPGILGYNICPRG WNGSLRLKRGSLPTEASCTT in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129267 mRNA. Translation: BAC98077.1. Different initiation.
CF539371 mRNA. No translation available.
AJ278123 mRNA. Translation: CAC34581.1.
AK034012 mRNA. Translation: BAC28546.1. Different initiation.
CCDSiCCDS37833.1. [Q8CC35-2]
CCDS50298.1. [Q8CC35-3]
UniGeneiMm.252321.
Mm.440740.

Genome annotation databases

EnsembliENSMUST00000097566; ENSMUSP00000095174; ENSMUSG00000043079. [Q8CC35-2]
ENSMUST00000115318; ENSMUSP00000110973; ENSMUSG00000043079. [Q8CC35-3]
ENSMUST00000130044; ENSMUSP00000121756; ENSMUSG00000043079. [Q8CC35-2]
ENSMUST00000143275; ENSMUSP00000125272; ENSMUSG00000043079. [Q8CC35-1]
ENSMUST00000155195; ENSMUSP00000117897; ENSMUSG00000043079. [Q8CC35-3]
UCSCiuc008far.2. mouse. [Q8CC35-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129267 mRNA. Translation: BAC98077.1. Different initiation.
CF539371 mRNA. No translation available.
AJ278123 mRNA. Translation: CAC34581.1.
AK034012 mRNA. Translation: BAC28546.1. Different initiation.
CCDSiCCDS37833.1. [Q8CC35-2]
CCDS50298.1. [Q8CC35-3]
UniGeneiMm.252321.
Mm.440740.

3D structure databases

ProteinModelPortaliQ8CC35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CC35. 2 interactors.
STRINGi10090.ENSMUSP00000095174.

PTM databases

iPTMnetiQ8CC35.
PhosphoSitePlusiQ8CC35.

Proteomic databases

PaxDbiQ8CC35.
PeptideAtlasiQ8CC35.
PRIDEiQ8CC35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097566; ENSMUSP00000095174; ENSMUSG00000043079. [Q8CC35-2]
ENSMUST00000115318; ENSMUSP00000110973; ENSMUSG00000043079. [Q8CC35-3]
ENSMUST00000130044; ENSMUSP00000121756; ENSMUSG00000043079. [Q8CC35-2]
ENSMUST00000143275; ENSMUSP00000125272; ENSMUSG00000043079. [Q8CC35-1]
ENSMUST00000155195; ENSMUSP00000117897; ENSMUSG00000043079. [Q8CC35-3]
UCSCiuc008far.2. mouse. [Q8CC35-1]

Organism-specific databases

MGIiMGI:1099446. Synpo.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IHBK. Eukaryota.
ENOG4111RGH. LUCA.
GeneTreeiENSGT00530000063754.
HOGENOMiHOG000132998.
HOVERGENiHBG056954.
InParanoidiQ8CC35.
PhylomeDBiQ8CC35.
TreeFamiTF330867.

Miscellaneous databases

ChiTaRSiSynpo. mouse.
PROiQ8CC35.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043079.
CleanExiMM_SYNPO.
ExpressionAtlasiQ8CC35. baseline and differential.
GenevisibleiQ8CC35. MM.

Family and domain databases

InterProiIPR028753. Synpo.
[Graphical view]
PANTHERiPTHR24217:SF13. PTHR24217:SF13. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSYNPO_MOUSE
AccessioniPrimary (citable) accession number: Q8CC35
Secondary accession number(s): Q99JI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Synpo deficient mice develop normally, but they lack the spine apparatuses. Adult mice have an impairment of spatial learning in the radial arm maze test.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.