Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Abl interactor 1

Gene

Abi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. In vitro, at least isoform 2 and isoform 4 suppress the transforming activity of Abelson murine leukemia virus (v-Abl) after overexpression in fibroblasts. May play a role in regulation EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.6 Publications

GO - Molecular functioni

GO - Biological processi

  • dendrite morphogenesis Source: MGI
  • lamellipodium morphogenesis Source: MGI
  • megakaryocyte development Source: MGI
  • peptidyl-tyrosine phosphorylation Source: MGI
  • somitogenesis Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Abl interactor 1
Alternative name(s):
Abelson interactor 1
Short name:
Abi-1
Ablphilin-1
Eps8 SH3 domain-binding protein
Short name:
Eps8-binding protein
Spectrin SH3 domain-binding protein 1
e3B1
Gene namesi
Name:Abi1
Synonyms:Ssh3bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:104913. Abi1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cell leading edge Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • filopodium Source: MGI
  • growth cone Source: UniProtKB-SubCell
  • intracellular Source: MGI
  • lamellipodium Source: MGI
  • neuron projection Source: BHF-UCL
  • nucleus Source: UniProtKB-SubCell
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
  • SCAR complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001917882 – 481Abl interactor 1Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei53PhosphotyrosineBy similarity1
Modified residuei174PhosphothreonineBy similarity1
Modified residuei178PhosphothreonineBy similarity1
Modified residuei183PhosphoserineCombined sources1
Modified residuei187PhosphoserineBy similarity1
Modified residuei213PhosphotyrosineCombined sources1
Modified residuei215PhosphothreonineCombined sources1
Modified residuei216PhosphoserineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei225PhosphoserineBy similarity1
Modified residuei292PhosphoserineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Modified residuei428PhosphotyrosineCombined sources1
Modified residuei439PhosphoserineBy similarity1
Modified residuei480PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CBW3.
PRIDEiQ8CBW3.

PTM databases

iPTMnetiQ8CBW3.
PhosphoSitePlusiQ8CBW3.

Expressioni

Tissue specificityi

Widely expressed with highest levels in bone marrow, spleen, brain, testes, and embryonic brain. In adult brain prominently expressed in the neocortex, hippocampus and dentate gyrus.2 Publications

Developmental stagei

Detected at E10 and E12 in developing brain, but does not appear more prominent in the neuroepithelium compared to the surrounding tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000058835.
CleanExiMM_ABI1.
ExpressionAtlasiQ8CBW3. baseline and differential.
GenevisibleiQ8CBW3. MM.

Interactioni

Subunit structurei

Interacts with ENAH, Abelson murine leukemia virus V-ABL, ABL1, STX1A, SNAP25, VAMP2, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1. Interacts with EPS8, SOS1, SOS2, GRB2, SPTA1, and the first SH3 domain of NCK1 (By similarity). Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Interacts (via SH3 domain) with SHANK2 and SHANK3, but not SHANK1; the interaction is direct. Interacts with the heterodimer MYC:MAX; the interaction may enhance MYC:MAX transcriptional activity. Interacts with FNBP1L (via the SH3 domain), WASF2, and CDC42, but only in the presence of FNBP1L (By similarity).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Eps8Q085092EBI-375511,EBI-375596
Sos2Q023842EBI-375511,EBI-395573

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197905. 10 interactors.
DIPiDIP-29534N.
IntActiQ8CBW3. 30 interactors.
MINTiMINT-128698.
STRINGi10090.ENSMUSP00000118491.

Structurei

3D structure databases

ProteinModelPortaliQ8CBW3.
SMRiQ8CBW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 107t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63
Domaini419 – 478SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 79Required for binding to WASF1Add BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi337 – 391Pro-richAdd BLAST55

Domaini

The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.

Sequence similaritiesi

Belongs to the ABI family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2546. Eukaryota.
ENOG410Y0MH. LUCA.
GeneTreeiENSGT00390000003756.
HOGENOMiHOG000293213.
HOVERGENiHBG050446.
InParanoidiQ8CBW3.
OMAiMPMFDDS.
PhylomeDBiQ8CBW3.
TreeFamiTF314303.

Family and domain databases

InterProiIPR028457. ABI.
IPR028456. ABI1.
IPR012849. Abl-interactor_HHR_dom.
IPR001452. SH3_domain.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR10460. PTHR10460. 2 hits.
PTHR10460:SF2. PTHR10460:SF2. 2 hits.
PfamiPF07815. Abi_HHR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CBW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET
60 70 80 90 100
KAYTTQSLAS VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH
110 120 130 140 150
KEKVARREIG ILTTNKNTSR THKIIAPANM ERPVRYIRKP IDYTVLDDVG
160 170 180 190 200
HGVKWLKAKH GNNQPARTGT LSRTNPPTQK PPSPPVSGRG TLGRNTPYKT
210 220 230 240 250
LEPVKPPTVP NDYMTSPARL GSQHSPGRTA SLNQRPRTHS GSSGGSGSRE
260 270 280 290 300
NSGSSSIGIP IAVPTPSPPT AGPAAPGAAP GSQYGTMTRQ ISRHNSTTSS
310 320 330 340 350
TSSGGYRRTP SVAAQFSAQP HVNGGPLYSQ NSISVAPPPP PMPQLTPQIP
360 370 380 390 400
LTGFVARVQE NIADSPTPPP PPPPDDIPMF DDSPPPPPPP PVDYEDEEAA
410 420 430 440 450
VVQYSDPYAD GDPAWAPKNY IEKVVAIYDY TKDKDDELSF KEGAIIYVIK
460 470 480
KNDDGWFEGV CNRVTGLFPG NYVESIMHYT D
Length:481
Mass (Da):52,288
Last modified:January 23, 2007 - v3
Checksum:i1654A1E89438BC1D
GO
Isoform 2 (identifier: Q8CBW3-2) [UniParc]FASTAAdd to basket
Also known as: short

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.
     274-274: Missing.
     333-361: Missing.

Show »
Length:446
Mass (Da):48,512
Checksum:i805A2F6072F1D24B
GO
Isoform 3 (identifier: Q8CBW3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.

Show »
Length:476
Mass (Da):51,661
Checksum:iB7E38AA1E9ED7FA6
GO
Isoform 4 (identifier: Q8CBW3-4) [UniParc]FASTAAdd to basket
Also known as: long

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.
     274-274: Missing.

Show »
Length:475
Mass (Da):51,590
Checksum:iA0A613AAD091B0F0
GO
Isoform 5 (identifier: Q8CBW3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.
     274-362: AAPGAAPGSQ...GFVARVQENI → V

Show »
Length:388
Mass (Da):42,548
Checksum:i27FAB95E05BCA655
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313A → T in AAH04657 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010756154 – 158Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 5 Publications5
Alternative sequenceiVSP_022636274 – 362AAPGA…VQENI → V in isoform 5. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_010757274Missing in isoform 2 and isoform 4. 3 Publications1
Alternative sequenceiVSP_010758333 – 361Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420251 mRNA. Translation: AAL16036.1.
AY033645 mRNA. Translation: AAK59381.1.
AK034476 mRNA. Translation: BAC28722.1.
AK152061 mRNA. Translation: BAE30917.1.
AK152184 mRNA. Translation: BAE31015.1.
AK151026 mRNA. Translation: BAE30044.1.
BC004657 mRNA. Translation: AAH04657.1.
U17698 mRNA. Translation: AAB00373.1. Sequence problems.
CCDSiCCDS38058.1. [Q8CBW3-5]
CCDS38059.1. [Q8CBW3-3]
CCDS38060.1. [Q8CBW3-2]
CCDS38061.1. [Q8CBW3-1]
CCDS50513.1. [Q8CBW3-4]
RefSeqiNP_001070658.1. NM_001077190.3. [Q8CBW3-1]
NP_001070660.1. NM_001077192.3. [Q8CBW3-2]
NP_001070661.1. NM_001077193.3. [Q8CBW3-5]
NP_031406.2. NM_007380.4. [Q8CBW3-4]
UniGeneiMm.205647.
Mm.249752.

Genome annotation databases

EnsembliENSMUST00000114544; ENSMUSP00000110191; ENSMUSG00000058835. [Q8CBW3-5]
ENSMUST00000123948; ENSMUSP00000118491; ENSMUSG00000058835. [Q8CBW3-1]
ENSMUST00000126112; ENSMUSP00000117335; ENSMUSG00000058835. [Q8CBW3-3]
ENSMUST00000140164; ENSMUSP00000120462; ENSMUSG00000058835. [Q8CBW3-4]
ENSMUST00000149719; ENSMUSP00000120621; ENSMUSG00000058835. [Q8CBW3-2]
GeneIDi11308.
KEGGimmu:11308.
UCSCiuc008ins.2. mouse. [Q8CBW3-1]
uc008inw.2. mouse. [Q8CBW3-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420251 mRNA. Translation: AAL16036.1.
AY033645 mRNA. Translation: AAK59381.1.
AK034476 mRNA. Translation: BAC28722.1.
AK152061 mRNA. Translation: BAE30917.1.
AK152184 mRNA. Translation: BAE31015.1.
AK151026 mRNA. Translation: BAE30044.1.
BC004657 mRNA. Translation: AAH04657.1.
U17698 mRNA. Translation: AAB00373.1. Sequence problems.
CCDSiCCDS38058.1. [Q8CBW3-5]
CCDS38059.1. [Q8CBW3-3]
CCDS38060.1. [Q8CBW3-2]
CCDS38061.1. [Q8CBW3-1]
CCDS50513.1. [Q8CBW3-4]
RefSeqiNP_001070658.1. NM_001077190.3. [Q8CBW3-1]
NP_001070660.1. NM_001077192.3. [Q8CBW3-2]
NP_001070661.1. NM_001077193.3. [Q8CBW3-5]
NP_031406.2. NM_007380.4. [Q8CBW3-4]
UniGeneiMm.205647.
Mm.249752.

3D structure databases

ProteinModelPortaliQ8CBW3.
SMRiQ8CBW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197905. 10 interactors.
DIPiDIP-29534N.
IntActiQ8CBW3. 30 interactors.
MINTiMINT-128698.
STRINGi10090.ENSMUSP00000118491.

PTM databases

iPTMnetiQ8CBW3.
PhosphoSitePlusiQ8CBW3.

Proteomic databases

PaxDbiQ8CBW3.
PRIDEiQ8CBW3.

Protocols and materials databases

DNASUi11308.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114544; ENSMUSP00000110191; ENSMUSG00000058835. [Q8CBW3-5]
ENSMUST00000123948; ENSMUSP00000118491; ENSMUSG00000058835. [Q8CBW3-1]
ENSMUST00000126112; ENSMUSP00000117335; ENSMUSG00000058835. [Q8CBW3-3]
ENSMUST00000140164; ENSMUSP00000120462; ENSMUSG00000058835. [Q8CBW3-4]
ENSMUST00000149719; ENSMUSP00000120621; ENSMUSG00000058835. [Q8CBW3-2]
GeneIDi11308.
KEGGimmu:11308.
UCSCiuc008ins.2. mouse. [Q8CBW3-1]
uc008inw.2. mouse. [Q8CBW3-5]

Organism-specific databases

CTDi10006.
MGIiMGI:104913. Abi1.

Phylogenomic databases

eggNOGiKOG2546. Eukaryota.
ENOG410Y0MH. LUCA.
GeneTreeiENSGT00390000003756.
HOGENOMiHOG000293213.
HOVERGENiHBG050446.
InParanoidiQ8CBW3.
OMAiMPMFDDS.
PhylomeDBiQ8CBW3.
TreeFamiTF314303.

Enzyme and pathway databases

ReactomeiR-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

ChiTaRSiAbi1. mouse.
PROiQ8CBW3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058835.
CleanExiMM_ABI1.
ExpressionAtlasiQ8CBW3. baseline and differential.
GenevisibleiQ8CBW3. MM.

Family and domain databases

InterProiIPR028457. ABI.
IPR028456. ABI1.
IPR012849. Abl-interactor_HHR_dom.
IPR001452. SH3_domain.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR10460. PTHR10460. 2 hits.
PTHR10460:SF2. PTHR10460:SF2. 2 hits.
PfamiPF07815. Abi_HHR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABI1_MOUSE
AccessioniPrimary (citable) accession number: Q8CBW3
Secondary accession number(s): Q3U8V0
, Q60747, Q91ZM5, Q923I9, Q99KH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.