Reviewed, UniProtKB/Swiss-Prot Q8CBQ5 (P4K2B_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase type 2-beta
    EC=2.7.1.67
Alternative name(s):
    Phosphatidylinositol 4-kinase type II-beta

Gene names

Name:

Pi4k2b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Enzyme regulation	Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity By similarity.
Subcellular location	Cytoplasm By similarity. Membrane By similarity. Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CBQ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CBQ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     84-129: ETNTFLEDPEFADIVLKAEQAIEIGVFPERISQGSSGSYFVKDSKR → GPELCPFYSRSLCYKTTTALGYY

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Phosphatidylinositol 4-kinase type 2-beta		PRO_0000285165
Regions
	Domain	117 – 424	308	PI3K/PI4K
Amino acid modifications
	Modified residue	37	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	84 – 129	46	ETNTF…KDSKR → GPELCPFYSRSLCYKTTTAL GYY in isoform 2.		VSP_024828
Experimental info
	Sequence conflict	199	1	W → L in AAL04155. Ref.1
	Sequence conflict	199	1	W → L in BAB27819. Ref.3
	Sequence conflict	229 – 236	8	GRKFHRIG → AESPSDR in AAN37399. Ref.2
	Sequence conflict	244	1	F → S in AAN37399. Ref.2
	Sequence conflict	244	1	F → S in BAB30411. Ref.3
	Sequence conflict	261	1	F → Y in AAN37399. Ref.2
	Sequence conflict	261	1	F → Y in BAB30411. Ref.3
	Sequence conflict	399	1	R → K in AAH10257. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B5CE80948E5B8954	FASTA	469	53,478
	10 20 30 40 50 60 MAEACEPTRP SEDEDEEREP LLPRVAWAQP RRVAPGSAVR MQADEGADVL REPATDEPPA 70 80 90 100 110 120 VSGEGSISAS LSTELDRTRT TSSETNTFLE DPEFADIVLK AEQAIEIGVF PERISQGSSG 130 140 150 160 170 180 SYFVKDSKRN IIGVFKPKSE EPYGQLNPKW TKYVHKVCCP CCFGRGCLLP NQGYLSEAGA 190 200 210 220 230 240 YLVDVKLNLG IVPKTKVVWL VSETFNYSAI DRAKSRGKKY ALEKVPKVGR KFHRIGLPPK 250 260 270 280 290 300 VGSFQLFVKD YKEAEYWLRR FEAEPLPENI RKQFQSQFEK LVILDYIIRN TDRGNDNWLV 310 320 330 340 350 360 KYDEMKYAKK IESEESNWID NKQLLIKIAA IDNGLAFPFK HPDEWRAYPF HWAWLPQAKV 370 380 390 400 410 420 PFSEETRNLI LPYISDMNFV QDLCEDLYEL FKTDKGFDRA AFENQMSVMR GQILNLTQAL 430 440 450 460 RDGKSPMQLA QMPCVIVECS KSGSQGRVVH LGSSFTQTVH CRKPFFSSW
	Isoform 2. Checksum: D32EB3277F0B160F	FASTA	446	50,962
	10 20 30 40 50 60 MAEACEPTRP SEDEDEEREP LLPRVAWAQP RRVAPGSAVR MQADEGADVL REPATDEPPA 70 80 90 100 110 120 VSGEGSISAS LSTELDRTRT TSSGPELCPF YSRSLCYKTT TALGYYNIIG VFKPKSEEPY 130 140 150 160 170 180 GQLNPKWTKY VHKVCCPCCF GRGCLLPNQG YLSEAGAYLV DVKLNLGIVP KTKVVWLVSE 190 200 210 220 230 240 TFNYSAIDRA KSRGKKYALE KVPKVGRKFH RIGLPPKVGS FQLFVKDYKE AEYWLRRFEA 250 260 270 280 290 300 EPLPENIRKQ FQSQFEKLVI LDYIIRNTDR GNDNWLVKYD EMKYAKKIES EESNWIDNKQ 310 320 330 340 350 360 LLIKIAAIDN GLAFPFKHPD EWRAYPFHWA WLPQAKVPFS EETRNLILPY ISDMNFVQDL 370 380 390 400 410 420 CEDLYELFKT DKGFDRAAFE NQMSVMRGQI LNLTQALRDG KSPMQLAQMP CVIVECSKSG 430 440 SQGRVVHLGS SFTQTVHCRK PFFSSW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta isoform of mouse type II phosphatidylinositol 4-kinase (PI4KIIbeta)." Hong W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J.
[2]	"A gene from our subtracted mouse compacted embryo cDNA library." Li W., Lu G. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Kunmingbai.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Testis and Urinary bladder.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Embryo and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF411321 mRNA. Translation: AAL04155.1. AY148879 mRNA. Translation: AAN37399.1. AK011751 mRNA. Translation: BAB27819.1. AK016754 mRNA. Translation: BAB30411.1. AK035523 mRNA. Translation: BAC29091.1. AK170730 mRNA. Translation: BAE41986.1. BC010257 mRNA. Translation: AAH10257.1. BC062144 mRNA. Translation: AAH62144.1.
IPI	IPI00136722. IPI00317443.
RefSeq	NP_080227.2. NP_083020.2.
UniGene	Mm.248647
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8CBQ5.
Proteomic databases
PRIDE	Q8CBQ5.
Genome annotation databases
Ensembl	ENSMUST00000031081; ENSMUSP00000031081; ENSMUSG00000029186; Mus musculus. [Genome view] ENSMUST00000031082; ENSMUSP00000031082; ENSMUSG00000029186; Mus musculus. [Genome view]
GeneID	67073.
KEGG	mmu:67073.
UCSC	uc008xkq.1. mouse.
Organism-specific databases
CTD	67073.
MGI	MGI:1914323. Pi4k2b.
Phylogenomic databases
eggNOG	roNOG05931.
HOGENOM	HBG714361.
HOVERGEN	Q8CBQ5.
InParanoid	Q8CBQ5.
OMA	AEQAIEF.
OrthoDB	EOG9NW24V.
PhylomeDB	Q8CBQ5.
Enzyme and pathway databases
BRENDA	2.7.1.67. 244.
Gene expression databases
ArrayExpress	Q8CBQ5.
Bgee	Q8CBQ5.
CleanEx	MM_PI4K2B.
Genevestigator	Q8CBQ5.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. [Graphical view]
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	323492.
SOURCE	Search...

Entry information

Entry name

P4K2B_MOUSE

Accession

Primary (citable) accession number: Q8CBQ5
Secondary accession number(s): Q91Z30, Q9D072, Q9D471

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)