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Protein

E3 ubiquitin-protein ligase RNF170

Gene

Rnf170

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that plays an essential role in stimulus-induced inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) ubiquitination and degradation via the endoplasmic reticulum-associated degradation (ERAD) pathway. Also involved in ITPR1 turnover in resting cells.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri115 – 15844RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF170 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 170
Gene namesi
Name:Rnf170
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1924983. Rnf170.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252LumenalSequence analysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence analysisAdd
BLAST
Topological domaini74 – 229156CytoplasmicSequence analysisAdd
BLAST
Transmembranei230 – 25021HelicalSequence analysisAdd
BLAST
Topological domaini251 – 2511LumenalSequence analysis
Transmembranei252 – 27221HelicalSequence analysisAdd
BLAST
Topological domaini273 – 28614CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi130 – 1301C → S: Loss of E3 ligase activity. 1 Publication
Mutagenesisi132 – 1321H → S: Loss of E3 ligase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286E3 ubiquitin-protein ligase RNF170PRO_0000280701Add
BLAST

Proteomic databases

MaxQBiQ8CBG9.
PaxDbiQ8CBG9.
PRIDEiQ8CBG9.

PTM databases

PhosphoSiteiQ8CBG9.

Expressioni

Gene expression databases

BgeeiQ8CBG9.
CleanExiMM_RNF170.
ExpressionAtlasiQ8CBG9. baseline and differential.
GenevisibleiQ8CBG9. MM.

Interactioni

Subunit structurei

Constitutively associated with the ERLIN1/ERLIN 2 complex. Interacts with activated ITPR1.1 Publication

Protein-protein interaction databases

BioGridi218881. 2 interactions.
STRINGi10090.ENSMUSP00000014022.

Structurei

3D structure databases

ProteinModelPortaliQ8CBG9.
SMRiQ8CBG9. Positions 112-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri115 – 15844RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG2164. Eukaryota.
ENOG410XTFS. LUCA.
GeneTreeiENSGT00390000017123.
HOGENOMiHOG000290642.
HOVERGENiHBG093906.
InParanoidiQ8CBG9.
KOiK15707.
OMAiDMSCPVC.
OrthoDBiEOG7WX0B3.
PhylomeDBiQ8CBG9.
TreeFamiTF328342.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010652. DUF1232.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF06803. DUF1232. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CBG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQRYWRFQDN KIQDICFGVL GESWIQRPVM ARYYSEGQSL QQDDSFIEGV
60 70 80 90 100
SDQVLVAVVV SLALTATLLY ALLRNVQQNI HPENQELVRV LREQFQTEQD
110 120 130 140 150
VPAPARQQFY TEMYCPICLH QASFPVETNC GHLFCGSCII AYWRYGSWLG
160 170 180 190 200
AISCPICRQT VTLLLTVFGE DDQSQDVIRL RQDVNDYNRR FSGQPRSIME
210 220 230 240 250
RIMDLPTLLR HAFREVFSVG GLFWMFRIRI MLCLMGAFFY LISPLDFVPE
260 270 280
ALFGILGFLD DFFVIFLLLI YISIMYREVI TQRLTR
Length:286
Mass (Da):33,414
Last modified:March 1, 2003 - v1
Checksum:i17294042B9C5CD49
GO
Isoform 2 (identifier: Q8CBG9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-74: Missing.

Note: No experimental confirmation available.
Show »
Length:239
Mass (Da):28,301
Checksum:iA65DEAC072DE4145
GO
Isoform 3 (identifier: Q8CBG9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-216: SCIIAYWRYG...TLLRHAFREV → EQIMSDLVRV...CLAIMQKPWA
     217-286: Missing.

Note: No experimental confirmation available.
Show »
Length:216
Mass (Da):24,858
Checksum:i420F4A164BF20E6F
GO
Isoform 4 (identifier: Q8CBG9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-142: GSCIIAY → VLQLFPH
     143-286: Missing.

Note: No experimental confirmation available.
Show »
Length:142
Mass (Da):16,525
Checksum:iCF63A92FE00A8B77
GO
Isoform 5 (identifier: Q8CBG9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-140: GSCII → ENCGF
     141-286: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):16,240
Checksum:i3920A869F2A7D13F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051A → V in BAC31147 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei28 – 7447Missing in isoform 2. 1 PublicationVSP_023858Add
BLAST
Alternative sequencei136 – 1427GSCIIAY → VLQLFPH in isoform 4. 1 PublicationVSP_023859
Alternative sequencei136 – 1405GSCII → ENCGF in isoform 5. 1 PublicationVSP_023860
Alternative sequencei137 – 21680SCIIA…AFREV → EQIMSDLVRVTSLRPLWIGS GLDCSFSFYHIYVLRRVDCF TSPALVATQIIAYCLVYSIL RSEDIAQLVECLAIMQKPWA in isoform 3. 1 PublicationVSP_023861Add
BLAST
Alternative sequencei141 – 286146Missing in isoform 5. 1 PublicationVSP_023862Add
BLAST
Alternative sequencei143 – 286144Missing in isoform 4. 1 PublicationVSP_023863Add
BLAST
Alternative sequencei217 – 28670Missing in isoform 3. 1 PublicationVSP_023864Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036051 mRNA. Translation: BAC29287.1.
AK042063 mRNA. Translation: BAC31147.1.
AK044579 mRNA. Translation: BAC31988.1.
AK080591 mRNA. Translation: BAC37951.1.
AK152760 mRNA. Translation: BAE31474.1.
CH466580 Genomic DNA. Translation: EDL32805.1.
BC031383 mRNA. Translation: AAH31383.1.
BC114210 mRNA. Translation: AAI14211.1.
BC138930 mRNA. Translation: AAI38931.1.
BC145718 mRNA. Translation: AAI45719.1.
CCDSiCCDS22207.1. [Q8CBG9-1]
RefSeqiNP_084241.1. NM_029965.2. [Q8CBG9-1]
XP_006509278.1. XM_006509215.2. [Q8CBG9-1]
XP_006509279.1. XM_006509216.2. [Q8CBG9-1]
XP_006509280.1. XM_006509217.2. [Q8CBG9-1]
XP_006509281.1. XM_006509218.2. [Q8CBG9-1]
UniGeneiMm.291906.

Genome annotation databases

EnsembliENSMUST00000014022; ENSMUSP00000014022; ENSMUSG00000013878. [Q8CBG9-1]
ENSMUST00000110575; ENSMUSP00000106204; ENSMUSG00000013878. [Q8CBG9-3]
ENSMUST00000110579; ENSMUSP00000106208; ENSMUSG00000013878. [Q8CBG9-5]
ENSMUST00000153528; ENSMUSP00000118689; ENSMUSG00000013878. [Q8CBG9-2]
GeneIDi77733.
KEGGimmu:77733.
UCSCiuc009lhl.1. mouse. [Q8CBG9-3]
uc009lhm.1. mouse. [Q8CBG9-1]
uc009lhp.1. mouse. [Q8CBG9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036051 mRNA. Translation: BAC29287.1.
AK042063 mRNA. Translation: BAC31147.1.
AK044579 mRNA. Translation: BAC31988.1.
AK080591 mRNA. Translation: BAC37951.1.
AK152760 mRNA. Translation: BAE31474.1.
CH466580 Genomic DNA. Translation: EDL32805.1.
BC031383 mRNA. Translation: AAH31383.1.
BC114210 mRNA. Translation: AAI14211.1.
BC138930 mRNA. Translation: AAI38931.1.
BC145718 mRNA. Translation: AAI45719.1.
CCDSiCCDS22207.1. [Q8CBG9-1]
RefSeqiNP_084241.1. NM_029965.2. [Q8CBG9-1]
XP_006509278.1. XM_006509215.2. [Q8CBG9-1]
XP_006509279.1. XM_006509216.2. [Q8CBG9-1]
XP_006509280.1. XM_006509217.2. [Q8CBG9-1]
XP_006509281.1. XM_006509218.2. [Q8CBG9-1]
UniGeneiMm.291906.

3D structure databases

ProteinModelPortaliQ8CBG9.
SMRiQ8CBG9. Positions 112-158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218881. 2 interactions.
STRINGi10090.ENSMUSP00000014022.

PTM databases

PhosphoSiteiQ8CBG9.

Proteomic databases

MaxQBiQ8CBG9.
PaxDbiQ8CBG9.
PRIDEiQ8CBG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014022; ENSMUSP00000014022; ENSMUSG00000013878. [Q8CBG9-1]
ENSMUST00000110575; ENSMUSP00000106204; ENSMUSG00000013878. [Q8CBG9-3]
ENSMUST00000110579; ENSMUSP00000106208; ENSMUSG00000013878. [Q8CBG9-5]
ENSMUST00000153528; ENSMUSP00000118689; ENSMUSG00000013878. [Q8CBG9-2]
GeneIDi77733.
KEGGimmu:77733.
UCSCiuc009lhl.1. mouse. [Q8CBG9-3]
uc009lhm.1. mouse. [Q8CBG9-1]
uc009lhp.1. mouse. [Q8CBG9-2]

Organism-specific databases

CTDi81790.
MGIiMGI:1924983. Rnf170.

Phylogenomic databases

eggNOGiKOG2164. Eukaryota.
ENOG410XTFS. LUCA.
GeneTreeiENSGT00390000017123.
HOGENOMiHOG000290642.
HOVERGENiHBG093906.
InParanoidiQ8CBG9.
KOiK15707.
OMAiDMSCPVC.
OrthoDBiEOG7WX0B3.
PhylomeDBiQ8CBG9.
TreeFamiTF328342.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8CBG9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CBG9.
CleanExiMM_RNF170.
ExpressionAtlasiQ8CBG9. baseline and differential.
GenevisibleiQ8CBG9. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010652. DUF1232.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF06803. DUF1232. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Brain cortex, Cerebellum, Retina and Thymus.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5).
    Strain: FVB/N.
    Tissue: Mammary tumor and Olfactory epithelium.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Pancreas and Testis.
  5. "RNF170 protein, an endoplasmic reticulum membrane ubiquitin ligase, mediates inositol 1,4,5-trisphosphate receptor ubiquitination and degradation."
    Lu J.P., Wang Y., Sliter D.A., Pearce M.M., Wojcikiewicz R.J.
    J. Biol. Chem. 286:24426-24433(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ERLIN1/ERLIN2 COMPLEX AND ITPR1, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-130 AND HIS-132.

Entry informationi

Entry nameiRN170_MOUSE
AccessioniPrimary (citable) accession number: Q8CBG9
Secondary accession number(s): A6H618
, Q3U796, Q78QX6, Q8C8S1, Q8C9I0, Q8K0H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-30 is the initiator. Initiation at Met-30 is supported by sequence similarity with mammalian orthologs and by a Kozak context more favorable compared to that at Met-1.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.