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Protein

E3 ubiquitin-protein ligase RNF170

Gene

Rnf170

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that plays an essential role in stimulus-induced inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) ubiquitination and degradation via the endoplasmic reticulum-associated degradation (ERAD) pathway. Also involved in ITPR1 turnover in resting cells.1 Publication

Caution

It is uncertain whether Met-1 or Met-30 is the initiator. Initiation at Met-30 is supported by sequence similarity with mammalian orthologs and by a Kozak context more favorable compared to that at Met-1.Curated

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri115 – 158RING-typePROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF170 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 170
RING-type E3 ubiquitin transferase RNF170Curated
Gene namesi
Name:Rnf170
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1924983 Rnf170

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52LumenalSequence analysisAdd BLAST52
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 229CytoplasmicSequence analysisAdd BLAST156
Transmembranei230 – 250HelicalSequence analysisAdd BLAST21
Topological domaini251LumenalSequence analysis1
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Topological domaini273 – 286CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi130C → S: Loss of E3 ligase activity. 1 Publication1
Mutagenesisi132H → S: Loss of E3 ligase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002807011 – 286E3 ubiquitin-protein ligase RNF170Add BLAST286

Proteomic databases

PaxDbiQ8CBG9
PeptideAtlasiQ8CBG9
PRIDEiQ8CBG9

PTM databases

PhosphoSitePlusiQ8CBG9

Expressioni

Gene expression databases

BgeeiENSMUSG00000013878
CleanExiMM_RNF170
ExpressionAtlasiQ8CBG9 baseline and differential
GenevisibleiQ8CBG9 MM

Interactioni

Subunit structurei

Constitutively associated with the ERLIN1/ERLIN 2 complex. Interacts with activated ITPR1.1 Publication

Protein-protein interaction databases

BioGridi218881, 2 interactors
STRINGi10090.ENSMUSP00000014022

Structurei

3D structure databases

ProteinModelPortaliQ8CBG9
SMRiQ8CBG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri115 – 158RING-typePROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG2164 Eukaryota
ENOG410XTFS LUCA
GeneTreeiENSGT00390000017123
HOGENOMiHOG000290642
HOVERGENiHBG093906
InParanoidiQ8CBG9
KOiK15707
OMAiMSCPVCL
OrthoDBiEOG091G0KJ3
PhylomeDBiQ8CBG9
TreeFamiTF328342

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR010652 DUF1232
IPR018957 Znf_C3HC4_RING-type
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF06803 DUF1232, 1 hit
PF00097 zf-C3HC4, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CBG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQRYWRFQDN KIQDICFGVL GESWIQRPVM ARYYSEGQSL QQDDSFIEGV
60 70 80 90 100
SDQVLVAVVV SLALTATLLY ALLRNVQQNI HPENQELVRV LREQFQTEQD
110 120 130 140 150
VPAPARQQFY TEMYCPICLH QASFPVETNC GHLFCGSCII AYWRYGSWLG
160 170 180 190 200
AISCPICRQT VTLLLTVFGE DDQSQDVIRL RQDVNDYNRR FSGQPRSIME
210 220 230 240 250
RIMDLPTLLR HAFREVFSVG GLFWMFRIRI MLCLMGAFFY LISPLDFVPE
260 270 280
ALFGILGFLD DFFVIFLLLI YISIMYREVI TQRLTR
Length:286
Mass (Da):33,414
Last modified:March 1, 2003 - v1
Checksum:i17294042B9C5CD49
GO
Isoform 2 (identifier: Q8CBG9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-74: Missing.

Note: No experimental confirmation available.
Show »
Length:239
Mass (Da):28,301
Checksum:iA65DEAC072DE4145
GO
Isoform 3 (identifier: Q8CBG9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-216: SCIIAYWRYG...TLLRHAFREV → EQIMSDLVRV...CLAIMQKPWA
     217-286: Missing.

Note: No experimental confirmation available.
Show »
Length:216
Mass (Da):24,858
Checksum:i420F4A164BF20E6F
GO
Isoform 4 (identifier: Q8CBG9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-142: GSCIIAY → VLQLFPH
     143-286: Missing.

Note: No experimental confirmation available.
Show »
Length:142
Mass (Da):16,525
Checksum:iCF63A92FE00A8B77
GO
Isoform 5 (identifier: Q8CBG9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-140: GSCII → ENCGF
     141-286: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):16,240
Checksum:i3920A869F2A7D13F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105A → V in BAC31147 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02385828 – 74Missing in isoform 2. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_023859136 – 142GSCIIAY → VLQLFPH in isoform 4. 1 Publication7
Alternative sequenceiVSP_023860136 – 140GSCII → ENCGF in isoform 5. 1 Publication5
Alternative sequenceiVSP_023861137 – 216SCIIA…AFREV → EQIMSDLVRVTSLRPLWIGS GLDCSFSFYHIYVLRRVDCF TSPALVATQIIAYCLVYSIL RSEDIAQLVECLAIMQKPWA in isoform 3. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_023862141 – 286Missing in isoform 5. 1 PublicationAdd BLAST146
Alternative sequenceiVSP_023863143 – 286Missing in isoform 4. 1 PublicationAdd BLAST144
Alternative sequenceiVSP_023864217 – 286Missing in isoform 3. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036051 mRNA Translation: BAC29287.1
AK042063 mRNA Translation: BAC31147.1
AK044579 mRNA Translation: BAC31988.1
AK080591 mRNA Translation: BAC37951.1
AK152760 mRNA Translation: BAE31474.1
CH466580 Genomic DNA Translation: EDL32805.1
BC031383 mRNA Translation: AAH31383.1
BC114210 mRNA Translation: AAI14211.1
BC138930 mRNA Translation: AAI38931.1
BC145718 mRNA Translation: AAI45719.1
CCDSiCCDS22207.1 [Q8CBG9-1]
RefSeqiNP_084241.1, NM_029965.2 [Q8CBG9-1]
XP_006509278.1, XM_006509215.3
XP_006509279.1, XM_006509216.3
XP_006509280.1, XM_006509217.3 [Q8CBG9-1]
XP_006509281.1, XM_006509218.3
UniGeneiMm.291906

Genome annotation databases

EnsembliENSMUST00000014022; ENSMUSP00000014022; ENSMUSG00000013878 [Q8CBG9-1]
ENSMUST00000110575; ENSMUSP00000106204; ENSMUSG00000013878 [Q8CBG9-3]
ENSMUST00000110579; ENSMUSP00000106208; ENSMUSG00000013878 [Q8CBG9-5]
ENSMUST00000153528; ENSMUSP00000118689; ENSMUSG00000013878 [Q8CBG9-2]
ENSMUST00000209300; ENSMUSP00000147842; ENSMUSG00000013878 [Q8CBG9-4]
GeneIDi77733
KEGGimmu:77733
UCSCiuc009lhl.1 mouse [Q8CBG9-3]
uc009lhm.1 mouse [Q8CBG9-1]
uc009lhp.1 mouse [Q8CBG9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRN170_MOUSE
AccessioniPrimary (citable) accession number: Q8CBG9
Secondary accession number(s): A6H618
, Q3U796, Q78QX6, Q8C8S1, Q8C9I0, Q8K0H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2003
Last modified: February 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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