Reviewed, UniProtKB/Swiss-Prot Q8CBF3 (EPHB1_MOUSE)

Names and origin

Protein names

Recommended name:
    Ephrin type-B receptor 1
    EC=2.7.10.1

Gene names

Name:

Ephb1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	984 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with EPHB6 and PRKCABP By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	camera-type eye morphogenesis Inferred from mutant phenotype. Source: MGI central nervous system projection neuron axonogenesis Inferred from direct assay. Source: MGI optic nerve morphogenesis Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro retinal ganglion cell axon guidance Inferred from direct assay. Source: MGI transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: MGI integral to plasma membrane Traceable author statement. Source: MGI membrane raft Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW axon guidance receptor activity Inferred from direct assay. Source: MGI transmembrane-ephrin receptor activity Traceable author statement. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CBF3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CBF3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     588-628: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 984	967	Ephrin type-B receptor 1		PRO_0000260317
Regions
	Topological domain	18 – 540	523	Extracellular Potential
	Transmembrane	541 – 563	23	Potential
	Topological domain	564 – 984	421	Cytoplasmic Potential
	Domain	323 – 424	102	Fibronectin type-III 1
	Domain	430 – 525	96	Fibronectin type-III 2
	Domain	619 – 882	264	Protein kinase
	Domain	911 – 975	65	SAM
	Nucleotide binding	625 – 633	9	ATP By similarity
	Motif	982 – 984	3	PDZ-binding Potential
	Compositional bias	183 – 319	137	Cys-rich
Sites
	Active site	744	1	Proton acceptor By similarity
	Binding site	651	1	ATP By similarity
Amino acid modifications
	Modified residue	928	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	968	1	Phosphoserine By similarity
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	588 – 628	41	Missing in isoform 2.		VSP_021595
Experimental info
	Sequence conflict	72	1	L → Q in BAE22386. Ref.1
	Sequence conflict	187	1	L → P in BAE22386. Ref.1
	Sequence conflict	194	1	K → I in AAH57301. Ref.3
	Sequence conflict	641	1	P → Q in BAC29348. Ref.1
	Sequence conflict	678	1	P → R in AAH57301. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E967019C82AA400A	FASTA	984	109,881
	10 20 30 40 50 60 MALDCLLLFL LASAVAAMEE TLMDTRTATA ELGWTANPAS GWEEVSGYDE NLNTIRTYQV 70 80 90 100 110 120 CNVFEPNQNN WLLTTFINRR GAHRIYTEMR FTVRDCSSLP NVPGSCKETF NLYYYETDSV 130 140 150 160 170 180 IATKKSAFWS EAPYLKVDTI AADESFSQVD FGGRLMKVNT EVRSFGPLTR NGFYLAFQDY 190 200 210 220 230 240 GACMSLLSVR VFFKKCPSIV QNFAVFPETM TGAESTSLVI ARGTCIPNAE EVDVPIKLYC 250 260 270 280 290 300 NGDGEWMVPI GRCTCKPGYE PENSVACKAC PAGTFKASQE AEGCSHCPSN SRSPSEASPI 310 320 330 340 350 360 CTCRTGYYRA DFDPPEVACT SVPSGPRNVI SIVNETSIIL EWHPPRETGG RDDVTYNIIC 370 380 390 400 410 420 KKCRADRRSC SRCDDNVEFV PRQLGLTECR VSISSLWAHT PYTFDIQAIN GVSSKSPFPP 430 440 450 460 470 480 QHVSVNITTN QAAPSTVPIM HQVSATMRSI TLSWPQPEQP NGIILDYEIR YYEKEHNEFN 490 500 510 520 530 540 SSMARSQTNT ARIDGLRPGM VYVVQVRART VAGYGKFSGK MCFQTLTDDD YKSELREQLP 550 560 570 580 590 600 LIAGSAAAGV VFVVSLVAIS IVCSRKRAYS KEAAYSDKLQ HYSTGRGSPG MKIYIDPFTY 610 620 630 640 650 660 EDPNEAVREF AKEIDVSFVK IEEVIGAGEF GEVYKGRLKL PGKREIYVAI KTLKAGYSEK 670 680 690 700 710 720 QRRDFLSEAS IMGQFDHPNI IRLEGVVTKS RPVMIITEFM ENGALDSFLR QNDGQFTVIQ 730 740 750 760 770 780 LVGMLRGIAA GMKYLSEMNY VHRDLAARNI LVNSNLVCKV SDFGLSRYLQ DDTSDPTYTS 790 800 810 820 830 840 SLGGKIPVRW TAPEAIAYRK FTSASDVWSY GIVMWEVMSF GERPYWDMSN QDVINAIEQD 850 860 870 880 890 900 YRLPPPMDCP AALHQLMLDC WQKDRNSRPR FAEIVNTLDK MIRNPASLKT VATITAVPSQ 910 920 930 940 950 960 PLLDRSIPDF TAFTTVDDWL SAIKMVQYRD SFLTAGFTSL QLVTQMTSED LLRIGVTLAG 970 980 HQKKILSSIH SMRVQMNQSP SVMA
	Isoform 2. Checksum: EF28BF1D7FE097C9	FASTA	943	105,294
	10 20 30 40 50 60 MALDCLLLFL LASAVAAMEE TLMDTRTATA ELGWTANPAS GWEEVSGYDE NLNTIRTYQV 70 80 90 100 110 120 CNVFEPNQNN WLLTTFINRR GAHRIYTEMR FTVRDCSSLP NVPGSCKETF NLYYYETDSV 130 140 150 160 170 180 IATKKSAFWS EAPYLKVDTI AADESFSQVD FGGRLMKVNT EVRSFGPLTR NGFYLAFQDY 190 200 210 220 230 240 GACMSLLSVR VFFKKCPSIV QNFAVFPETM TGAESTSLVI ARGTCIPNAE EVDVPIKLYC 250 260 270 280 290 300 NGDGEWMVPI GRCTCKPGYE PENSVACKAC PAGTFKASQE AEGCSHCPSN SRSPSEASPI 310 320 330 340 350 360 CTCRTGYYRA DFDPPEVACT SVPSGPRNVI SIVNETSIIL EWHPPRETGG RDDVTYNIIC 370 380 390 400 410 420 KKCRADRRSC SRCDDNVEFV PRQLGLTECR VSISSLWAHT PYTFDIQAIN GVSSKSPFPP 430 440 450 460 470 480 QHVSVNITTN QAAPSTVPIM HQVSATMRSI TLSWPQPEQP NGIILDYEIR YYEKEHNEFN 490 500 510 520 530 540 SSMARSQTNT ARIDGLRPGM VYVVQVRART VAGYGKFSGK MCFQTLTDDD YKSELREQLP 550 560 570 580 590 600 LIAGSAAAGV VFVVSLVAIS IVCSRKRAYS KEAAYSDKLQ HYSTGRGEFG EVYKGRLKLP 610 620 630 640 650 660 GKREIYVAIK TLKAGYSEKQ RRDFLSEASI MGQFDHPNII RLEGVVTKSR PVMIITEFME 670 680 690 700 710 720 NGALDSFLRQ NDGQFTVIQL VGMLRGIAAG MKYLSEMNYV HRDLAARNIL VNSNLVCKVS 730 740 750 760 770 780 DFGLSRYLQD DTSDPTYTSS LGGKIPVRWT APEAIAYRKF TSASDVWSYG IVMWEVMSFG 790 800 810 820 830 840 ERPYWDMSNQ DVINAIEQDY RLPPPMDCPA ALHQLMLDCW QKDRNSRPRF AEIVNTLDKM 850 860 870 880 890 900 IRNPASLKTV ATITAVPSQP LLDRSIPDFT AFTTVDDWLS AIKMVQYRDS FLTAGFTSLQ 910 920 930 940 LVTQMTSEDL LRIGVTLAGH QKKILSSIHS MRVQMNQSPS VMA

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cerebellum and Olfactory bulb.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK036148 mRNA. Translation: BAC29320.1. AK036211 mRNA. Translation: BAC29348.1. AK135018 mRNA. Translation: BAE22386.1. CT025594 , AC109247, AC132684, AC156635 Genomic DNA. Translation: CAM23741.1. BC057301 mRNA. Translation: AAH57301.1.
IPI	IPI00228309. IPI00808241.
RefSeq	NP_001161768.1. NP_775623.3.
UniGene	Mm.22897
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CBF3.
PTM databases
PhosphoSite	Q8CBF3.
Proteomic databases
PRIDE	Q8CBF3.
Genome annotation databases
Ensembl	ENSMUST00000035129; ENSMUSP00000035129; ENSMUSG00000032537; Mus musculus. [Genome view] ENSMUST00000085169; ENSMUSP00000082261; ENSMUSG00000032537; Mus musculus. [Genome view]
GeneID	270190.
KEGG	mmu:270190.
UCSC	uc009rfn.1. mouse.
Organism-specific databases
CTD	270190.
MGI	MGI:1096337. Ephb1.
Phylogenomic databases
eggNOG	roNOG09683.
HOGENOM	HBG755340.
HOVERGEN	Q8CBF3.
InParanoid	Q8CBF3.
OMA	NSVACKA.
PhylomeDB	Q8CBF3.
Enzyme and pathway databases
BRENDA	2.7.10.1. 244.
Gene expression databases
ArrayExpress	Q8CBF3.
Bgee	Q8CBF3.
CleanEx	MM_EPHB1.
Genevestigator	Q8CBF3.
GermOnline	ENSMUSG00000032537. Mus musculus.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR010993. SAM_homology. IPR013761. SAM_type. IPR021129. SAM_type1. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	393293.
SOURCE	Search...

Entry information

Entry name

EPHB1_MOUSE

Accession

Primary (citable) accession number: Q8CBF3
Secondary accession number(s): B1B1C2 , Q3UY27, Q6PG23, Q8CBE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)