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Protein

Nuclear receptor-interacting protein 1

Gene

Nrip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of ARNTL/BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC.7 Publications

GO - Molecular functioni

  • core promoter sequence-specific DNA binding Source: UniProtKB
  • estrogen receptor binding Source: MGI
  • glucocorticoid receptor binding Source: MGI
  • histone deacetylase binding Source: UniProtKB
  • nuclear hormone receptor binding Source: MGI
  • receptor binding Source: UniProtKB
  • retinoic acid receptor binding Source: UniProtKB
  • retinoid X receptor binding Source: UniProtKB
  • transcription coactivator activity Source: UniProtKB
  • transcription corepressor activity Source: MGI

GO - Biological processi

  • cellular response to estradiol stimulus Source: MGI
  • circadian regulation of gene expression Source: UniProtKB
  • lipid storage Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • ovarian follicle rupture Source: UniProtKB
  • ovulation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor, Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor-interacting protein 1
Alternative name(s):
Nuclear factor RIP140
Receptor-interacting protein 140
Gene namesi
Name:Nrip1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1315213. Nrip1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: MGI
  • histone deacetylase complex Source: UniProtKB
  • nuclear chromatin Source: MGI
  • nuclear speck Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1067N → G: Reduces binding to RAR, RXR and RAR/RXR. 1 Publication1
Mutagenesisi1068P → A: Abolishes binding to RAR, RXR and RAR/RXR. 1 Publication1
Mutagenesisi1073M → I: Reduces binding to RAR, RXR and RAR/RXR. 1 Publication1
Mutagenesisi1073M → K: Abolishes binding to RAR, RXR and RAR/RXR. 1 Publication1
Mutagenesisi1073M → L: Reduces binding to RAR, RXR and RAR/RXR. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579521 – 1161Nuclear receptor-interacting protein 1Add BLAST1161

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104Phosphoserine1 Publication1
Modified residuei111N6-acetyllysine1 Publication1
Modified residuei158N6-acetyllysine1 Publication1
Modified residuei207Phosphothreonine1 Publication1
Modified residuei218PhosphoserineBy similarity1
Modified residuei287N6-acetyllysine1 Publication1
Modified residuei311N6-acetyllysine1 Publication1
Modified residuei358Phosphoserine1 Publication1
Modified residuei380Phosphoserine1 Publication1
Modified residuei447N6-acetyllysineBy similarity1
Modified residuei482N6-acetyllysine1 Publication1
Modified residuei488Phosphoserine1 Publication1
Modified residuei519Phosphoserine1 Publication1
Modified residuei529N6-acetyllysine1 Publication1
Modified residuei531Phosphoserine1 Publication1
Modified residuei543Phosphoserine1 Publication1
Modified residuei565PhosphoserineBy similarity1
Modified residuei607N6-acetyllysine1 Publication1
Modified residuei672Phosphoserine1 Publication1
Cross-linki757Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei808PhosphoserineBy similarity1
Modified residuei932N6-acetyllysine1 Publication1
Modified residuei1003Phosphoserine1 Publication1
Cross-linki1108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH (By similarity). Acetylation regulates its nuclear translocation and corepressive activity.By similarity2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8CBD1.
PaxDbiQ8CBD1.
PeptideAtlasiQ8CBD1.
PRIDEiQ8CBD1.

PTM databases

iPTMnetiQ8CBD1.
PhosphoSitePlusiQ8CBD1.

Expressioni

Tissue specificityi

Expressed in the embryonic placenta. In the adult, expression is strong in the testis and brain. Also expressed at a high level in the white adipose tissue. Expressed constantly but at a weaker level in the adult heart, lung, stomach and kidney. Expressed moderately in the skeletal muscle. Expressed at a low level in the adult spleen, liver and brown adipose tissue. Expressed in the ovary at a high level in granulosa cells and at a lower level in the thecal and interstitial compartments.3 Publications

Gene expression databases

BgeeiENSMUSG00000048490.
CleanExiMM_NRIP1.
GenevisibleiQ8CBD1. MM.

Interactioni

Subunit structurei

Interacts with CTBP1, CTBP2, ERS1, HDAC1, HDAC2, HDAC5, HDAC6, NR2C2, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH (By similarity). Interacts with NR2C1 (sumoylated form and via the ligand-binding domain); the interaction results in promoting the repressor activity of NR2C1. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain. Interacts with ZNF366 (By similarity). Interacts with RORA.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC3O153792EBI-1771626,EBI-607682From a different organism.

GO - Molecular functioni

  • estrogen receptor binding Source: MGI
  • glucocorticoid receptor binding Source: MGI
  • histone deacetylase binding Source: UniProtKB
  • nuclear hormone receptor binding Source: MGI
  • receptor binding Source: UniProtKB
  • retinoic acid receptor binding Source: UniProtKB
  • retinoid X receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi234576. 11 interactors.
DIPiDIP-29280N.
IntActiQ8CBD1. 4 interactors.
STRINGi10090.ENSMUSP00000051726.

Structurei

3D structure databases

ProteinModelPortaliQ8CBD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 416Interaction with ZNF366By similarityAdd BLAST416
Regioni78 – 335Repression domain 1By similarityAdd BLAST258
Regioni411 – 701Repression domain 2By similarityAdd BLAST291
Regioni432 – 473Required for targeting to small nuclear fociBy similarityAdd BLAST42
Regioni736 – 886Repression domain 3By similarityAdd BLAST151
Regioni754 – 1161Interaction with ZNF366By similarityAdd BLAST408
Regioni1063 – 1076Ligand-dependent nuclear receptor bindingAdd BLAST14
Regioni1121 – 1161Repression domain 4By similarityAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 25LXXLL motif 1Sequence analysis5
Motifi133 – 137LXXLL motif 2Sequence analysis5
Motifi185 – 189LXXLL motif 3Sequence analysis5
Motifi267 – 271LXXLL motif 4Sequence analysis5
Motifi382 – 386LXXLL motif 5Sequence analysis5
Motifi441 – 447CTBP-binding; principal siteBy similarity7
Motifi501 – 505LXXLL motif 6Sequence analysis5
Motifi566 – 570CTBP-bindingBy similarity5
Motifi714 – 718LXXLL motif 7Sequence analysis5
Motifi820 – 824LXXLL motif 8Sequence analysis5
Motifi937 – 941LXXLL motif 9Sequence analysis5
Motifi947 – 951CTBP-bindingBy similarity5

Domaini

Contains at least 4 autonomous repression domains (RD1-4).By similarity
The Ligand-dependent nuclear receptor binding region is required for ligand-dependent interaction with RAAR and RXRB homo- and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFW7. Eukaryota.
ENOG410XPVS. LUCA.
GeneTreeiENSGT00390000007999.
HOGENOMiHOG000236277.
HOVERGENiHBG052667.
InParanoidiQ8CBD1.
KOiK17965.
OMAiVEKDLRC.
OrthoDBiEOG091G07UX.
PhylomeDBiQ8CBD1.
TreeFamiTF332210.

Family and domain databases

InterProiIPR026649. NRIP1.
IPR031405. NRIP1_RD1.
IPR031406. NRIP1_RD2.
IPR031407. NRIP1_RD3.
IPR031408. NRIP1_RD4.
[Graphical view]
PANTHERiPTHR15088. PTHR15088. 1 hit.
PfamiPF15687. NRIP1_repr_1. 1 hit.
PF15688. NRIP1_repr_2. 1 hit.
PF15689. NRIP1_repr_3. 1 hit.
PF15690. NRIP1_repr_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CBD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHGEELGSD VHQDSIVLTY LEGLLMHQAA GGSGTAINKK SAGHKEEDQN
60 70 80 90 100
FNLSGSAFPS CQSNGPTVST QTYQGSGMLH LKKARLLQSS EDWNAAKRKR
110 120 130 140 150
LSDSIVNLNV KKEALLAGMV DSVPKGKQDS TLLASLLQSF SSRLQTVALS
160 170 180 190 200
QQIRQSLKEQ GYALSHESLK VEKDLRCYGV ASSHLKTLLK KSKTKDQKSG
210 220 230 240 250
PTLPDVTPNL IRDSFVESSH PAVGQSGTKV MSEPLSCAAR LQAVASMVEK
260 270 280 290 300
RASPAASPKP SVACSQLALL LSSEAHLQQY SREHALKTQN AHQVASERLA
310 320 330 340 350
AMARLQENGQ KDVGSSQLSK GVSGHLNGQA RALPASKLVA NKNNAATFQS
360 370 380 390 400
PMGVVPSSPK NTSYKNSLER NNLKQAANNS LLLHLLKSQT IPTPMNGHSQ
410 420 430 440 450
NERASSFESS TPTTIDEYSD NNPSFTDDSS GDESSYSNCV PIDLSCKHRI
460 470 480 490 500
EKPEAERPVS LENLTQSLLN TWDPKIPGVD IKEDQDTSTN SKLNSHQKVT
510 520 530 540 550
LLQLLLGHKS EETVERNASP QDIHSDGTKF SPQNYTRTSV IESPSTNRTT
560 570 580 590 600
PVSTPPLYTA SQAESPINLS QHSLVIKWNS PPYACSTPAS KLTNTAPSHL
610 620 630 640 650
MDLTKGKESQ AEKPAPSEGA QNSATFSASK LLQNLAQCGL QSSGPGEEQR
660 670 680 690 700
PCKQLLSGNP DKPLGLIDRL NSPLLSNKTN AAEESKAFSS QPAGPEPGLP
710 720 730 740 750
GCEIENLLER RTVLQLLLGN SSKGKNEKKE KTPARDEAPQ EHSERAANEQ
760 770 780 790 800
ILMVKIKSEP CDDFQTHNTN LPLNHDAKSA PFLGVTPAIH RSTAALPVSE
810 820 830 840 850
DFKSEPASPQ DFSFSKNGLL SRLLRQNQES YPADEQDKSH RNSELPTLES
860 870 880 890 900
KNICMVPKKR KLYTEPLENP FKKMKNTAVD TANHHSGPEV LYGSLLHQEE
910 920 930 940 950
LKFSRNELDY KYPAGHSSAS DGDHRSWARE SKSFNVLKQL LLSENCVRDL
960 970 980 990 1000
SPHRSDSVPD TKKKGHKNNA PGSKPEFGIS SLNGLMYSSP QPGSCVTDHR
1010 1020 1030 1040 1050
TFSYPGMVKT PLSPPFPEHL GCVGSRPEPG LLNGCSVPGE KGPIKWVIAD
1060 1070 1080 1090 1100
MDKNEYEKDS PRLTKTNPIL YYMLQKGGGN SVTTQETQDK DIWREPASAE
1110 1120 1130 1140 1150
SLSQVTVKEE LLPAAETKAS FFNLRSPYNS HMGNNASRPH STNGEVYGLL
1160
GNALTIKKES E
Length:1,161
Mass (Da):126,337
Last modified:March 1, 2003 - v1
Checksum:i56ABD0C2A5AAC0A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106V → N AA sequence (PubMed:15879431).Curated1
Sequence conflicti107N → NN AA sequence (PubMed:15846843).Curated1
Sequence conflicti119 – 120MV → IL in AAC69611 (PubMed:9774688).Curated2
Sequence conflicti190K → R in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti197Q → E in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti200G → D in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti522D → V in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti596A → V in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti617S → N in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti629S → K AA sequence (PubMed:15879431).Curated1
Sequence conflicti674L → Q in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti818G → F in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti855M → I in BAE33634 (PubMed:16141072).Curated1
Sequence conflicti886S → R in AAC69611 (PubMed:9774688).Curated1
Sequence conflicti1049A → T in AAC69611 (PubMed:9774688).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053062 mRNA. Translation: AAC69611.1.
AK036273 mRNA. Translation: BAC29368.1.
AK084498 mRNA. Translation: BAC39197.1.
AK156233 mRNA. Translation: BAE33634.1.
BC060232 mRNA. Translation: AAH60232.1.
CCDSiCCDS28274.1.
RefSeqiNP_775616.1. NM_173440.2.
XP_006523113.1. XM_006523050.2.
XP_006523114.1. XM_006523051.3.
XP_006523117.1. XM_006523054.2.
XP_017172509.1. XM_017317020.1.
UniGeneiMm.447018.
Mm.455873.
Mm.74711.

Genome annotation databases

EnsembliENSMUST00000054178; ENSMUSP00000051726; ENSMUSG00000048490.
ENSMUST00000121927; ENSMUSP00000112959; ENSMUSG00000048490.
GeneIDi268903.
KEGGimmu:268903.
UCSCiuc007zrx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053062 mRNA. Translation: AAC69611.1.
AK036273 mRNA. Translation: BAC29368.1.
AK084498 mRNA. Translation: BAC39197.1.
AK156233 mRNA. Translation: BAE33634.1.
BC060232 mRNA. Translation: AAH60232.1.
CCDSiCCDS28274.1.
RefSeqiNP_775616.1. NM_173440.2.
XP_006523113.1. XM_006523050.2.
XP_006523114.1. XM_006523051.3.
XP_006523117.1. XM_006523054.2.
XP_017172509.1. XM_017317020.1.
UniGeneiMm.447018.
Mm.455873.
Mm.74711.

3D structure databases

ProteinModelPortaliQ8CBD1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234576. 11 interactors.
DIPiDIP-29280N.
IntActiQ8CBD1. 4 interactors.
STRINGi10090.ENSMUSP00000051726.

PTM databases

iPTMnetiQ8CBD1.
PhosphoSitePlusiQ8CBD1.

Proteomic databases

MaxQBiQ8CBD1.
PaxDbiQ8CBD1.
PeptideAtlasiQ8CBD1.
PRIDEiQ8CBD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054178; ENSMUSP00000051726; ENSMUSG00000048490.
ENSMUST00000121927; ENSMUSP00000112959; ENSMUSG00000048490.
GeneIDi268903.
KEGGimmu:268903.
UCSCiuc007zrx.1. mouse.

Organism-specific databases

CTDi8204.
MGIiMGI:1315213. Nrip1.

Phylogenomic databases

eggNOGiENOG410IFW7. Eukaryota.
ENOG410XPVS. LUCA.
GeneTreeiENSGT00390000007999.
HOGENOMiHOG000236277.
HOVERGENiHBG052667.
InParanoidiQ8CBD1.
KOiK17965.
OMAiVEKDLRC.
OrthoDBiEOG091G07UX.
PhylomeDBiQ8CBD1.
TreeFamiTF332210.

Miscellaneous databases

PROiQ8CBD1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048490.
CleanExiMM_NRIP1.
GenevisibleiQ8CBD1. MM.

Family and domain databases

InterProiIPR026649. NRIP1.
IPR031405. NRIP1_RD1.
IPR031406. NRIP1_RD2.
IPR031407. NRIP1_RD3.
IPR031408. NRIP1_RD4.
[Graphical view]
PANTHERiPTHR15088. PTHR15088. 1 hit.
PfamiPF15687. NRIP1_repr_1. 1 hit.
PF15688. NRIP1_repr_2. 1 hit.
PF15689. NRIP1_repr_3. 1 hit.
PF15690. NRIP1_repr_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRIP1_MOUSE
AccessioniPrimary (citable) accession number: Q8CBD1
Secondary accession number(s): Q3U166, Q8C3Y8, Q9Z2K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.