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Q8CBC4 (CNST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Consortin
Gene names
Name:Cnst
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for targeting of connexins to the plasma membrane By similarity.

Subunit structure

Interacts with connexins GJA1/CX43, GJB1/CX32, GJB2/CX26, GJB3/CX31, GJB6/CX30 and GJC1/CX45. Also interacts with GGA1 and GGA2. Does not interact with PANX1. Ref.3

Subcellular location

Cell membrane; Single-pass membrane protein By similarity. Golgi apparatustrans-Golgi network membrane; Single-pass membrane protein By similarity. Cytoplasmic vesiclesecretory vesicle By similarity. Note: Located predominantly in the trans-Golgi network. Probably trafficks between the trans-Golgi network and the cell membrane via the secretory pathway By similarity.

Sequence similarities

Belongs to the CNST family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Gga1Q8R0H92EBI-2615407,EBI-2616212
Gga2Q6P5E62EBI-2615407,EBI-2616239
Gjb2P219942EBI-2615407,EBI-2616119From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CBC4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CBC4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     128-197: DAVPVMQTQN...VAETYFQEED → EAFRLSLLSF...PSLRHGGFAL
     198-711: Missing.
Isoform 3 (identifier: Q8CBC4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-289: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Consortin
PRO_0000288907

Regions

Transmembrane651 – 67121Helical; Potential

Amino acid modifications

Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation2181N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 289289Missing in isoform 3.
VSP_038657
Alternative sequence128 – 19770DAVPV…FQEED → EAFRLSLLSFNKNKHFFWCC SAAAPVAPLCGSCIPFVPAV TVYREITTCLVCFLYRPHHG VLTLPSLRHGGFAL in isoform 2.
VSP_025821
Alternative sequence198 – 711514Missing in isoform 2.
VSP_025822

Experimental info

Mutagenesis558 – 5625DSDLL → NSDAA: Does not affect binding to connexins but results in their intracellular accumulation. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: B117F1FA8648E8EC

FASTA71176,866
        10         20         30         40         50         60 
MDDSDPPTYS LQIEPQDGCH PGDSVERRVT RLPSVSDENE NQLAGDGPAG LTTSEGAMGR 

        70         80         90        100        110        120 
ATVSEQDSLN NNESFPSSCE AAPTENAENT PSEGPKDDPP SLGQDQKLPA KRSPRAKKSS 

       130        140        150        160        170        180 
PKSAPPGDAV PVMQTQNATS QAAGEEEAAG VNANDPPKAP ALQPLFSLIR GEVAQMDSRA 

       190        200        210        220        230        240 
LPLFLHQVAE TYFQEEDYEK AMKFIQLERL YHEQLLANLS AIQEQWETKW KAVQPRTVTP 

       250        260        270        280        290        300 
LRNSEKGFNG EDFEQLAKIC TTHQDPLLSK LKTAPVEPSP ERKSLARAIM SEEAVGTEAA 

       310        320        330        340        350        360 
AKEPEIETCP STDPSGDRHE EEPQESSPGC HQMEWQTASP ELPGTAGKDH TEELPSSTNA 

       370        380        390        400        410        420 
TLDLHTQSLE TAGSRSGPAA ASNACKDSSC VPAPPTEDHC GVARDPKVAP PSESVAEQKL 

       430        440        450        460        470        480 
STGDDGALPG LISEGKYSQA HRKELCLPLQ DAFEALPRDQ PHSSEVAEPR QPDVTASDGK 

       490        500        510        520        530        540 
SAQSQAGLET GPESALCGDR KACDVSTLCL EVCMAPEERR DSEDRVSKET EDYLHSLLER 

       550        560        570        580        590        600 
CLKDAEDSLS YEDIQDDDSD LLQDLSPEEA SYSLQEDLPP DESTLSLDDL AKKIEIAEAI 

       610        620        630        640        650        660 
PAEGLVSILK KRNDTVGSHP AQMQQKPAKR RVRFQEIDDN LEQDEVGGGS CILLILLCIA 

       670        680        690        700        710 
TVFLSVGGTA LYCTLGNIES PVCTDFADNV DFYYTKLLQG VAGLKHWVYL S 

« Hide

Isoform 2 [UniParc].

Checksum: F439F01E6FF16D28
Show »

FASTA20121,495
Isoform 3 [UniParc].

Checksum: 63ECC182B66CFF30
Show »

FASTA42245,414

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Amnion, Aorta, Cerebellum and Vein.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: C57BL/6.
Tissue: Brain and Eye.
[3]"Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins."
del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C.
Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF ISOFORM 3, INTERACTION WITH GGA1; GGA2; GJA1; GJB1; GJB2; GJB3; GJB6 AND GJC1, MUTAGENESIS OF 558-562.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK036332 mRNA. Translation: BAC29388.1.
AK040666 mRNA. Translation: BAC30660.1.
AK040899 mRNA. Translation: BAC30736.1.
AK146681 mRNA. Translation: BAE27356.1.
BC034107 mRNA. Translation: AAH34107.1.
BC054802 mRNA. Translation: AAH54802.1.
RefSeqNP_666217.2. NM_146105.3.
UniGeneMm.87662.

3D structure databases

ProteinModelPortalQ8CBC4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8CBC4. 9 interactions.
STRING10090.ENSMUSP00000048205.

PTM databases

PhosphoSiteQ8CBC4.

Proteomic databases

PaxDbQ8CBC4.
PRIDEQ8CBC4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040706; ENSMUSP00000048205; ENSMUSG00000038949. [Q8CBC4-1]
GeneID226744.
KEGGmmu:226744.
UCSCuc007dvm.1. mouse. [Q8CBC4-2]
uc007dvo.1. mouse. [Q8CBC4-1]

Organism-specific databases

CTD163882.
MGIMGI:2445141. Cnst.

Phylogenomic databases

eggNOGNOG29134.
GeneTreeENSGT00390000005861.
HOGENOMHOG000111226.
HOVERGENHBG098332.
InParanoidQ8CBC4.
KOK17581.
OMAKHWIYLS.
OrthoDBEOG708VZD.
PhylomeDBQ8CBC4.
TreeFamTF351172.

Gene expression databases

BgeeQ8CBC4.
CleanExMM_9630058J23RIK.
GenevestigatorQ8CBC4.

Family and domain databases

InterProIPR028129. Consortin_C.
[Graphical view]
PfamPF15281. Consortin_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCNST. mouse.
NextBio378309.
PROQ8CBC4.
SOURCESearch...

Entry information

Entry nameCNST_MOUSE
AccessionPrimary (citable) accession number: Q8CBC4
Secondary accession number(s): Q8BFS5, Q8K053
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot