Q8CBC4 (CNST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Consortin

Gene names

Name:

Cnst

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	711 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for targeting of connexins to the plasma membrane By similarity.
Subunit structure	Interacts with connexins GJA1/CX43, GJB1/CX32, GJB2/CX26, GJB3/CX31, GJB6/CX30 and GJC1/CX45. Also interacts with GGA1 and GGA2. Does not interact with PANX1. Ref.3
Subcellular location	Cell membrane; Single-pass membrane protein By similarity. Golgi apparatus › trans-Golgi network membrane; Single-pass membrane protein By similarity. Cytoplasmic vesicle › secretory vesicle By similarity. Note: Located predominantly in the trans-Golgi network. Probably trafficks between the trans-Golgi network and the cell membrane via the secretory pathway By similarity.
Sequence similarities	Belongs to the CNST family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of Golgi to plasma membrane protein transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB protein complex Inferred from electronic annotation. Source: Compara trans-Golgi network Inferred from sequence or structural similarity. Source: UniProtKB transport vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	connexin binding Inferred from direct assay Ref.3. Source: UniProtKB phosphatase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gga1	Q8R0H9	2	EBI-2615407,EBI-2616212
Gga2	Q6P5E6	2	EBI-2615407,EBI-2616239
Gjb2	P21994	2	EBI-2615407,EBI-2616119	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CBC4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CBC4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     128-197: DAVPVMQTQN...VAETYFQEED → EAFRLSLLSF...PSLRHGGFAL
     198-711: Missing.

Isoform 3 (identifier: Q8CBC4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-289: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 711	711	Consortin		PRO_0000288907
Regions
	Transmembrane	651 – 671	21	Helical; Potential
Amino acid modifications
	Glycosylation	137	1	N-linked (GlcNAc...) Potential
	Glycosylation	218	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 289	289	Missing in isoform 3.		VSP_038657
	Alternative sequence	128 – 197	70	DAVPV…FQEED → EAFRLSLLSFNKNKHFFWCC SAAAPVAPLCGSCIPFVPAV TVYREITTCLVCFLYRPHHG VLTLPSLRHGGFAL in isoform 2.		VSP_025821
	Alternative sequence	198 – 711	514	Missing in isoform 2.		VSP_025822
Experimental info
	Mutagenesis	558 – 562	5	DSDLL → NSDAA: Does not affect binding to connexins but results in their intracellular accumulation. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B117F1FA8648E8EC	FASTA	711	76,866
	10 20 30 40 50 60 MDDSDPPTYS LQIEPQDGCH PGDSVERRVT RLPSVSDENE NQLAGDGPAG LTTSEGAMGR 70 80 90 100 110 120 ATVSEQDSLN NNESFPSSCE AAPTENAENT PSEGPKDDPP SLGQDQKLPA KRSPRAKKSS 130 140 150 160 170 180 PKSAPPGDAV PVMQTQNATS QAAGEEEAAG VNANDPPKAP ALQPLFSLIR GEVAQMDSRA 190 200 210 220 230 240 LPLFLHQVAE TYFQEEDYEK AMKFIQLERL YHEQLLANLS AIQEQWETKW KAVQPRTVTP 250 260 270 280 290 300 LRNSEKGFNG EDFEQLAKIC TTHQDPLLSK LKTAPVEPSP ERKSLARAIM SEEAVGTEAA 310 320 330 340 350 360 AKEPEIETCP STDPSGDRHE EEPQESSPGC HQMEWQTASP ELPGTAGKDH TEELPSSTNA 370 380 390 400 410 420 TLDLHTQSLE TAGSRSGPAA ASNACKDSSC VPAPPTEDHC GVARDPKVAP PSESVAEQKL 430 440 450 460 470 480 STGDDGALPG LISEGKYSQA HRKELCLPLQ DAFEALPRDQ PHSSEVAEPR QPDVTASDGK 490 500 510 520 530 540 SAQSQAGLET GPESALCGDR KACDVSTLCL EVCMAPEERR DSEDRVSKET EDYLHSLLER 550 560 570 580 590 600 CLKDAEDSLS YEDIQDDDSD LLQDLSPEEA SYSLQEDLPP DESTLSLDDL AKKIEIAEAI 610 620 630 640 650 660 PAEGLVSILK KRNDTVGSHP AQMQQKPAKR RVRFQEIDDN LEQDEVGGGS CILLILLCIA 670 680 690 700 710 TVFLSVGGTA LYCTLGNIES PVCTDFADNV DFYYTKLLQG VAGLKHWVYL S
	Isoform 2 [UniParc]. Checksum: F439F01E6FF16D28	FASTA	201	21,495
	10 20 30 40 50 60 MDDSDPPTYS LQIEPQDGCH PGDSVERRVT RLPSVSDENE NQLAGDGPAG LTTSEGAMGR 70 80 90 100 110 120 ATVSEQDSLN NNESFPSSCE AAPTENAENT PSEGPKDDPP SLGQDQKLPA KRSPRAKKSS 130 140 150 160 170 180 PKSAPPGEAF RLSLLSFNKN KHFFWCCSAA APVAPLCGSC IPFVPAVTVY REITTCLVCF 190 200 LYRPHHGVLT LPSLRHGGFA L
	Isoform 3 [UniParc]. Checksum: 63ECC182B66CFF30	FASTA	422	45,414
	10 20 30 40 50 60 MSEEAVGTEA AAKEPEIETC PSTDPSGDRH EEEPQESSPG CHQMEWQTAS PELPGTAGKD 70 80 90 100 110 120 HTEELPSSTN ATLDLHTQSL ETAGSRSGPA AASNACKDSS CVPAPPTEDH CGVARDPKVA 130 140 150 160 170 180 PPSESVAEQK LSTGDDGALP GLISEGKYSQ AHRKELCLPL QDAFEALPRD QPHSSEVAEP 190 200 210 220 230 240 RQPDVTASDG KSAQSQAGLE TGPESALCGD RKACDVSTLC LEVCMAPEER RDSEDRVSKE 250 260 270 280 290 300 TEDYLHSLLE RCLKDAEDSL SYEDIQDDDS DLLQDLSPEE ASYSLQEDLP PDESTLSLDD 310 320 330 340 350 360 LAKKIEIAEA IPAEGLVSIL KKRNDTVGSH PAQMQQKPAK RRVRFQEIDD NLEQDEVGGG 370 380 390 400 410 420 SCILLILLCI ATVFLSVGGT ALYCTLGNIE SPVCTDFADN VDFYYTKLLQ GVAGLKHWVY LS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Amnion, Aorta, Cerebellum and Vein.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6. Tissue: Brain and Eye.
[3]	"Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins." del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C. Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF ISOFORM 3, INTERACTION WITH GGA1; GGA2; GJA1; GJB1; GJB2; GJB3; GJB6 AND GJC1, MUTAGENESIS OF 558-562.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK036332 mRNA. Translation: BAC29388.1. AK040666 mRNA. Translation: BAC30660.1. AK040899 mRNA. Translation: BAC30736.1. AK146681 mRNA. Translation: BAE27356.1. BC034107 mRNA. Translation: AAH34107.1. BC054802 mRNA. Translation: AAH54802.1.
IPI	IPI00228289. IPI00475239. IPI00955089.
RefSeq	NP_666217.2. NM_146105.3.
UniGene	Mm.87662.
3D structure databases
ProteinModelPortal	Q8CBC4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CBC4. 9 interactions.
STRING	10090.ENSMUSP00000048205.
PTM databases
PhosphoSite	Q8CBC4.
Proteomic databases
PaxDb	Q8CBC4.
PRIDE	Q8CBC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000040706; ENSMUSP00000048205; ENSMUSG00000038949.
GeneID	226744.
KEGG	mmu:226744.
UCSC	uc007dvm.1. mouse. uc007dvo.1. mouse.
Organism-specific databases
CTD	163882.
MGI	MGI:2445141. Cnst.
Phylogenomic databases
eggNOG	NOG29134.
GeneTree	ENSGT00390000005861.
HOGENOM	HOG000111226.
HOVERGEN	HBG098332.
InParanoid	Q8CBC4.
OMA	NELQQPD.
OrthoDB	EOG4B8JCH.
Gene expression databases
Bgee	Q8CBC4.
CleanEx	MM_9630058J23RIK.
Genevestigator	Q8CBC4.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	CNST. mouse.
NextBio	378309.
SOURCE	Search...

Entry information

Entry name

CNST_MOUSE

Accession

Primary (citable) accession number: Q8CBC4
Secondary accession number(s): Q8BFS5, Q8K053

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program