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Protein

Ras association domain-containing protein 4

Gene

Rassf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Potential tumor suppressor. May act as a KRAS effector protein. May promote apoptosis and cell cycle arrest.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 4
Gene namesi
Name:Rassf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2386853. Rassf4.

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002403991 – 322Ras association domain-containing protein 4Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei142PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CB96.
PeptideAtlasiQ8CB96.
PRIDEiQ8CB96.

PTM databases

iPTMnetiQ8CB96.
PhosphoSitePlusiQ8CB96.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042129.
ExpressionAtlasiQ8CB96. baseline and differential.
GenevisibleiQ8CB96. MM.

Interactioni

Subunit structurei

Interacts directly with activated KRAS in a GTP-dependent manner.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048267.

Structurei

3D structure databases

ProteinModelPortaliQ8CB96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 264Ras-associatingPROSITE-ProRule annotationAdd BLAST90
Domaini271 – 318SARAHPROSITE-ProRule annotationAdd BLAST48

Sequence similaritiesi

Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SARAH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4239. Eukaryota.
ENOG410Y2N1. LUCA.
GeneTreeiENSGT00510000046426.
HOGENOMiHOG000231738.
HOVERGENiHBG054302.
InParanoidiQ8CB96.
KOiK09851.
OMAiMFLMEAD.
OrthoDBiEOG091G0BOA.
PhylomeDBiQ8CB96.
TreeFamiTF319243.

Family and domain databases

InterProiIPR033614. C-RASSF.
IPR000159. RA_dom.
IPR033622. RASSF4.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR22738. PTHR22738. 1 hit.
PTHR22738:SF4. PTHR22738:SF4. 1 hit.
PfamiPF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CB96-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKEACSSSSH VPVSDSKYIL KSELLSLLKT YNCYHEGRSF QLRHREEEGT
60 70 80 90 100
LIIEGLLNIA WGLRRPIRLQ MQDDRERVHL PSATWVPERL SYLQKEASPQ
110 120 130 140 150
DSKVPTEEPG TQPANKAEVS GDSSGALEGE EEEVPQLMRT KSDASCIIQR
160 170 180 190 200
RSKSRAPSEA QKIRRHRFSI NGHFYNHKTS VFTPAYGSVT NVRVNSTMTT
210 220 230 240 250
QQVLTLLLNK FRVEDGPSEF ALYTVHESGE QTKLKDCEYP LISRILHGPC
260 270 280 290 300
EKIVKIFLME ADLSEEVPHD VAQYIKFEMP VLDSFVEKLK EEEEREIIKL
310 320
TMKFQALRLT MLQRLEQLVE AK
Length:322
Mass (Da):36,934
Last modified:March 1, 2003 - v1
Checksum:iC8CEE2610DDEFCC5
GO
Isoform 2 (identifier: Q8CB96-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-322: Missing.

Show »
Length:303
Mass (Da):34,664
Checksum:i6E868DD20F300C2A
GO
Isoform 3 (identifier: Q8CB96-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-276: TSVFTPAYGS...VPHDVAQYIK → VKSPSALSWA...IQHSSHSSIR
     277-322: Missing.

Show »
Length:216
Mass (Da):24,598
Checksum:iE75461E9D35D985E
GO

Sequence cautioni

The sequence BAE42308 differs from that shown. Reason: Frameshift at position 285.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153K → E in BAE42308 (PubMed:16141072).Curated1
Sequence conflicti291E → G in BAE37757 (PubMed:16141072).Curated1
Sequence conflicti295R → I in AAH60709 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019360179 – 276TSVFT…AQYIK → VKSPSALSWALKLRVQELFP STEERAPNIQHSSHSSIR in isoform 3. 1 PublicationAdd BLAST98
Alternative sequenceiVSP_019361277 – 322Missing in isoform 3. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_019362304 – 322Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036528 mRNA. Translation: BAC29463.1.
AK160584 mRNA. Translation: BAE35891.1.
AK164357 mRNA. Translation: BAE37757.1.
AK170955 mRNA. Translation: BAE42138.1.
AK171198 mRNA. Translation: BAE42308.1. Frameshift.
AK139284 mRNA. Translation: BAE23943.1.
BC023245 mRNA. Translation: AAH23245.1.
BC060709 mRNA. Translation: AAH60709.1.
CCDSiCCDS20460.1. [Q8CB96-1]
RefSeqiNP_835146.3. NM_178045.4. [Q8CB96-1]
UniGeneiMm.257931.

Genome annotation databases

EnsembliENSMUST00000035842; ENSMUSP00000048267; ENSMUSG00000042129. [Q8CB96-1]
ENSMUST00000203029; ENSMUSP00000144786; ENSMUSG00000042129. [Q8CB96-2]
GeneIDi213391.
KEGGimmu:213391.
UCSCiuc009dkm.2. mouse. [Q8CB96-1]
uc009dko.3. mouse. [Q8CB96-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036528 mRNA. Translation: BAC29463.1.
AK160584 mRNA. Translation: BAE35891.1.
AK164357 mRNA. Translation: BAE37757.1.
AK170955 mRNA. Translation: BAE42138.1.
AK171198 mRNA. Translation: BAE42308.1. Frameshift.
AK139284 mRNA. Translation: BAE23943.1.
BC023245 mRNA. Translation: AAH23245.1.
BC060709 mRNA. Translation: AAH60709.1.
CCDSiCCDS20460.1. [Q8CB96-1]
RefSeqiNP_835146.3. NM_178045.4. [Q8CB96-1]
UniGeneiMm.257931.

3D structure databases

ProteinModelPortaliQ8CB96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048267.

PTM databases

iPTMnetiQ8CB96.
PhosphoSitePlusiQ8CB96.

Proteomic databases

PaxDbiQ8CB96.
PeptideAtlasiQ8CB96.
PRIDEiQ8CB96.

Protocols and materials databases

DNASUi213391.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035842; ENSMUSP00000048267; ENSMUSG00000042129. [Q8CB96-1]
ENSMUST00000203029; ENSMUSP00000144786; ENSMUSG00000042129. [Q8CB96-2]
GeneIDi213391.
KEGGimmu:213391.
UCSCiuc009dkm.2. mouse. [Q8CB96-1]
uc009dko.3. mouse. [Q8CB96-3]

Organism-specific databases

CTDi83937.
MGIiMGI:2386853. Rassf4.

Phylogenomic databases

eggNOGiKOG4239. Eukaryota.
ENOG410Y2N1. LUCA.
GeneTreeiENSGT00510000046426.
HOGENOMiHOG000231738.
HOVERGENiHBG054302.
InParanoidiQ8CB96.
KOiK09851.
OMAiMFLMEAD.
OrthoDBiEOG091G0BOA.
PhylomeDBiQ8CB96.
TreeFamiTF319243.

Miscellaneous databases

ChiTaRSiRassf4. mouse.
PROiQ8CB96.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042129.
ExpressionAtlasiQ8CB96. baseline and differential.
GenevisibleiQ8CB96. MM.

Family and domain databases

InterProiIPR033614. C-RASSF.
IPR000159. RA_dom.
IPR033622. RASSF4.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR22738. PTHR22738. 1 hit.
PTHR22738:SF4. PTHR22738:SF4. 1 hit.
PfamiPF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASF4_MOUSE
AccessioniPrimary (citable) accession number: Q8CB96
Secondary accession number(s): Q3TBK1
, Q3TPH9, Q3TUT0, Q6P9L7, Q8CIM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.