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Protein

Ras association domain-containing protein 4

Gene

Rassf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Potential tumor suppressor. May act as a KRAS effector protein. May promote apoptosis and cell cycle arrest.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 4
Gene namesi
Name:Rassf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2386853. Rassf4.

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Ras association domain-containing protein 4PRO_0000240399Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei142 – 1421PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CB96.
MaxQBiQ8CB96.
PaxDbiQ8CB96.
PRIDEiQ8CB96.

PTM databases

PhosphoSiteiQ8CB96.

Expressioni

Gene expression databases

BgeeiQ8CB96.
ExpressionAtlasiQ8CB96. baseline and differential.
GenevisibleiQ8CB96. MM.

Interactioni

Subunit structurei

Interacts directly with activated KRAS in a GTP-dependent manner.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048267.

Structurei

3D structure databases

ProteinModelPortaliQ8CB96.
SMRiQ8CB96. Positions 188-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini175 – 26490Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini271 – 31848SARAHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SARAH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4239. Eukaryota.
ENOG410Y2N1. LUCA.
GeneTreeiENSGT00510000046426.
HOGENOMiHOG000231738.
HOVERGENiHBG054302.
InParanoidiQ8CB96.
KOiK09851.
OMAiCEKIARM.
OrthoDBiEOG7DVDBF.
PhylomeDBiQ8CB96.
TreeFamiTF319243.

Family and domain databases

InterProiIPR000159. RA_dom.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CB96-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKEACSSSSH VPVSDSKYIL KSELLSLLKT YNCYHEGRSF QLRHREEEGT
60 70 80 90 100
LIIEGLLNIA WGLRRPIRLQ MQDDRERVHL PSATWVPERL SYLQKEASPQ
110 120 130 140 150
DSKVPTEEPG TQPANKAEVS GDSSGALEGE EEEVPQLMRT KSDASCIIQR
160 170 180 190 200
RSKSRAPSEA QKIRRHRFSI NGHFYNHKTS VFTPAYGSVT NVRVNSTMTT
210 220 230 240 250
QQVLTLLLNK FRVEDGPSEF ALYTVHESGE QTKLKDCEYP LISRILHGPC
260 270 280 290 300
EKIVKIFLME ADLSEEVPHD VAQYIKFEMP VLDSFVEKLK EEEEREIIKL
310 320
TMKFQALRLT MLQRLEQLVE AK
Length:322
Mass (Da):36,934
Last modified:March 1, 2003 - v1
Checksum:iC8CEE2610DDEFCC5
GO
Isoform 2 (identifier: Q8CB96-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-322: Missing.

Show »
Length:303
Mass (Da):34,664
Checksum:i6E868DD20F300C2A
GO
Isoform 3 (identifier: Q8CB96-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-276: TSVFTPAYGS...VPHDVAQYIK → VKSPSALSWA...IQHSSHSSIR
     277-322: Missing.

Show »
Length:216
Mass (Da):24,598
Checksum:iE75461E9D35D985E
GO

Sequence cautioni

The sequence BAE42308.1 differs from that shown. Reason: Frameshift at position 285. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531K → E in BAE42308 (PubMed:16141072).Curated
Sequence conflicti291 – 2911E → G in BAE37757 (PubMed:16141072).Curated
Sequence conflicti295 – 2951R → I in AAH60709 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei179 – 27698TSVFT…AQYIK → VKSPSALSWALKLRVQELFP STEERAPNIQHSSHSSIR in isoform 3. 1 PublicationVSP_019360Add
BLAST
Alternative sequencei277 – 32246Missing in isoform 3. 1 PublicationVSP_019361Add
BLAST
Alternative sequencei304 – 32219Missing in isoform 2. 1 PublicationVSP_019362Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036528 mRNA. Translation: BAC29463.1.
AK160584 mRNA. Translation: BAE35891.1.
AK164357 mRNA. Translation: BAE37757.1.
AK170955 mRNA. Translation: BAE42138.1.
AK171198 mRNA. Translation: BAE42308.1. Frameshift.
AK139284 mRNA. Translation: BAE23943.1.
BC023245 mRNA. Translation: AAH23245.1.
BC060709 mRNA. Translation: AAH60709.1.
CCDSiCCDS20460.1. [Q8CB96-1]
RefSeqiNP_835146.3. NM_178045.4. [Q8CB96-1]
UniGeneiMm.257931.

Genome annotation databases

EnsembliENSMUST00000035842; ENSMUSP00000048267; ENSMUSG00000042129. [Q8CB96-1]
ENSMUST00000203029; ENSMUSP00000144786; ENSMUSG00000042129. [Q8CB96-2]
GeneIDi213391.
KEGGimmu:213391.
UCSCiuc009dkm.2. mouse. [Q8CB96-1]
uc009dko.3. mouse. [Q8CB96-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036528 mRNA. Translation: BAC29463.1.
AK160584 mRNA. Translation: BAE35891.1.
AK164357 mRNA. Translation: BAE37757.1.
AK170955 mRNA. Translation: BAE42138.1.
AK171198 mRNA. Translation: BAE42308.1. Frameshift.
AK139284 mRNA. Translation: BAE23943.1.
BC023245 mRNA. Translation: AAH23245.1.
BC060709 mRNA. Translation: AAH60709.1.
CCDSiCCDS20460.1. [Q8CB96-1]
RefSeqiNP_835146.3. NM_178045.4. [Q8CB96-1]
UniGeneiMm.257931.

3D structure databases

ProteinModelPortaliQ8CB96.
SMRiQ8CB96. Positions 188-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048267.

PTM databases

PhosphoSiteiQ8CB96.

Proteomic databases

EPDiQ8CB96.
MaxQBiQ8CB96.
PaxDbiQ8CB96.
PRIDEiQ8CB96.

Protocols and materials databases

DNASUi213391.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035842; ENSMUSP00000048267; ENSMUSG00000042129. [Q8CB96-1]
ENSMUST00000203029; ENSMUSP00000144786; ENSMUSG00000042129. [Q8CB96-2]
GeneIDi213391.
KEGGimmu:213391.
UCSCiuc009dkm.2. mouse. [Q8CB96-1]
uc009dko.3. mouse. [Q8CB96-3]

Organism-specific databases

CTDi83937.
MGIiMGI:2386853. Rassf4.

Phylogenomic databases

eggNOGiKOG4239. Eukaryota.
ENOG410Y2N1. LUCA.
GeneTreeiENSGT00510000046426.
HOGENOMiHOG000231738.
HOVERGENiHBG054302.
InParanoidiQ8CB96.
KOiK09851.
OMAiCEKIARM.
OrthoDBiEOG7DVDBF.
PhylomeDBiQ8CB96.
TreeFamiTF319243.

Miscellaneous databases

ChiTaRSiRassf4. mouse.
NextBioi373933.
PROiQ8CB96.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CB96.
ExpressionAtlasiQ8CB96. baseline and differential.
GenevisibleiQ8CB96. MM.

Family and domain databases

InterProiIPR000159. RA_dom.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone, Cerebellum and Spinal ganglion.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J and FVB/N.
    Tissue: Brain and Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiRASF4_MOUSE
AccessioniPrimary (citable) accession number: Q8CB96
Secondary accession number(s): Q3TBK1
, Q3TPH9, Q3TUT0, Q6P9L7, Q8CIM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.