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Q8CAY6

- THIC_MOUSE

UniProt

Q8CAY6 - THIC_MOUSE

Protein

Acetyl-CoA acetyltransferase, cytosolic

Gene

Acat2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 acetyl-CoA = CoA + acetoacetyl-CoA.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Acyl-thioester intermediateBy similarity
    Active sitei353 – 3531Proton acceptorPROSITE-ProRule annotation
    Active sitei383 – 3831Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. acetyl-CoA C-acetyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-CoA acetyltransferase, cytosolic (EC:2.3.1.9)
    Alternative name(s):
    Cytosolic acetoacetyl-CoA thiolase
    Gene namesi
    Name:Acat2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:87871. Acat2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397Acetyl-CoA acetyltransferase, cytosolicPRO_0000206410Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei200 – 2001N6-acetyllysineBy similarity
    Modified residuei233 – 2331N6-acetyllysineBy similarity
    Modified residuei235 – 2351N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8CAY6.
    PaxDbiQ8CAY6.
    PRIDEiQ8CAY6.

    2D gel databases

    REPRODUCTION-2DPAGEQ8CAY6.

    PTM databases

    PhosphoSiteiQ8CAY6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CAY6.
    BgeeiQ8CAY6.
    CleanExiMM_ACAT2.
    GenevestigatoriQ8CAY6.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiQ8CAY6. 2 interactions.
    MINTiMINT-1864806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CAY6.
    SMRiQ8CAY6. Positions 4-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiolase family.Curated

    Phylogenomic databases

    eggNOGiCOG0183.
    GeneTreeiENSGT00390000009412.
    HOGENOMiHOG000012238.
    HOVERGENiHBG003112.
    InParanoidiQ8CAY6.
    KOiK00626.
    OMAiKRCLSRA.
    OrthoDBiEOG7JQBNG.
    PhylomeDBiQ8CAY6.
    TreeFamiTF300650.

    Family and domain databases

    Gene3Di3.40.47.10. 4 hits.
    InterProiIPR002155. Thiolase.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    IPR020610. Thiolase_AS.
    IPR020617. Thiolase_C.
    IPR020613. Thiolase_CS.
    IPR020616. Thiolase_N.
    [Graphical view]
    PfamiPF02803. Thiolase_C. 1 hit.
    PF00108. Thiolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
    PROSITEiPS00737. THIOLASE_2. 1 hit.
    PS00099. THIOLASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CAY6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAGSDPVVI VSAARTAIGS FNGALSTVPV HEMGTTVIKE VLQRAKVAPE    50
    EVSEVIFGHV LTAGCGQNPT RQASVGAGIP YSVPAWSCQM ICGSGLKAVC 100
    LAAQSIAMGD STIVVAGGME NMSKAPHLTH LRTGVRMGEV PLADSILCDG 150
    LTDAFHNYHM GITAENVAKK WQVSREAQDK VAVLSQNRAE HAQKAGHFDK 200
    EIVPVLVSSR KGLTEVKIDE FPRHGSNLEA MGKLKPYFLT DGTGTVTPAN 250
    ASGMNDGAAA VVLMKKTEAE RRMLKPLARI VSWSQAGVEP SVMGVGPIPA 300
    IKQAVAKAGW SLEDVDLFEI NEAFAAVSAA IAKELGLNPE KVNIDGGAIA 350
    LGHPLGASGC RILVTLLHTL ERVGGTRGVA ALCIGGGMGV AMCVQRG 397
    Length:397
    Mass (Da):41,298
    Last modified:September 13, 2004 - v2
    Checksum:iAABC2DF22F4EE930
    GO

    Sequence cautioni

    The sequence BAB28763.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061I → L in AAH12496. (PubMed:15489334)Curated
    Sequence conflicti115 – 1195VAGGM → CLTGK(PubMed:2055458)Curated
    Sequence conflicti125 – 1251A → P in AAA76575. (PubMed:2055458)Curated
    Sequence conflicti191 – 1911H → T in AAA76575. (PubMed:2055458)Curated
    Sequence conflicti230 – 2301A → D in BAB28763. (PubMed:16141072)Curated
    Sequence conflicti233 – 2331K → T in AAA76575. (PubMed:2055458)Curated
    Sequence conflicti262 – 2621V → L in BAB28763. (PubMed:16141072)Curated
    Sequence conflicti266 – 2661K → E in BAC29776. (PubMed:16141072)Curated
    Sequence conflicti340 – 3401E → G in AAA76575. (PubMed:2055458)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013275 mRNA. Translation: BAB28763.1. Different initiation.
    AK037249 mRNA. Translation: BAC29776.1.
    AK166813 mRNA. Translation: BAE39040.1.
    AK167234 mRNA. Translation: BAE39358.1.
    BC004823 mRNA. Translation: AAH04823.2.
    BC012496 mRNA. Translation: AAH12496.2.
    M35797 mRNA. Translation: AAA76575.1.
    CCDSiCCDS37437.1.
    PIRiA53174.
    RefSeqiNP_033364.2. NM_009338.3.
    UniGeneiMm.439711.

    Genome annotation databases

    EnsembliENSMUST00000007005; ENSMUSP00000007005; ENSMUSG00000023832.
    GeneIDi110460.
    KEGGimmu:110460.
    UCSCiuc008alp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013275 mRNA. Translation: BAB28763.1 . Different initiation.
    AK037249 mRNA. Translation: BAC29776.1 .
    AK166813 mRNA. Translation: BAE39040.1 .
    AK167234 mRNA. Translation: BAE39358.1 .
    BC004823 mRNA. Translation: AAH04823.2 .
    BC012496 mRNA. Translation: AAH12496.2 .
    M35797 mRNA. Translation: AAA76575.1 .
    CCDSi CCDS37437.1.
    PIRi A53174.
    RefSeqi NP_033364.2. NM_009338.3.
    UniGenei Mm.439711.

    3D structure databases

    ProteinModelPortali Q8CAY6.
    SMRi Q8CAY6. Positions 4-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8CAY6. 2 interactions.
    MINTi MINT-1864806.

    PTM databases

    PhosphoSitei Q8CAY6.

    2D gel databases

    REPRODUCTION-2DPAGE Q8CAY6.

    Proteomic databases

    MaxQBi Q8CAY6.
    PaxDbi Q8CAY6.
    PRIDEi Q8CAY6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000007005 ; ENSMUSP00000007005 ; ENSMUSG00000023832 .
    GeneIDi 110460.
    KEGGi mmu:110460.
    UCSCi uc008alp.1. mouse.

    Organism-specific databases

    CTDi 39.
    MGIi MGI:87871. Acat2.

    Phylogenomic databases

    eggNOGi COG0183.
    GeneTreei ENSGT00390000009412.
    HOGENOMi HOG000012238.
    HOVERGENi HBG003112.
    InParanoidi Q8CAY6.
    KOi K00626.
    OMAi KRCLSRA.
    OrthoDBi EOG7JQBNG.
    PhylomeDBi Q8CAY6.
    TreeFami TF300650.

    Miscellaneous databases

    NextBioi 364019.
    PROi Q8CAY6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CAY6.
    Bgeei Q8CAY6.
    CleanExi MM_ACAT2.
    Genevestigatori Q8CAY6.

    Family and domain databases

    Gene3Di 3.40.47.10. 4 hits.
    InterProi IPR002155. Thiolase.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    IPR020610. Thiolase_AS.
    IPR020617. Thiolase_C.
    IPR020613. Thiolase_CS.
    IPR020616. Thiolase_N.
    [Graphical view ]
    Pfami PF02803. Thiolase_C. 1 hit.
    PF00108. Thiolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    TIGRFAMsi TIGR01930. AcCoA-C-Actrans. 1 hit.
    PROSITEi PS00737. THIOLASE_2. 1 hit.
    PS00099. THIOLASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Blastocyst, Embryo and Skin.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Mammary gland and Retina.
    3. "Isolation and characterization of a cDNA clone corresponding to the mouse T-complex gene Tcp-1x."
      Dudley K., Shanahan F., Burtenshaw M., Evans E.P., Ruddy S., Lyon M.F.
      Genet. Res. 57:147-152(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-397.
      Strain: C57BL/6J.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 342-372, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiTHIC_MOUSE
    AccessioniPrimary (citable) accession number: Q8CAY6
    Secondary accession number(s): Q3TJY7
    , Q62292, Q96EB7, Q99K88, Q9CYV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3