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Protein

Acetyl-CoA acetyltransferase, cytosolic

Gene

Acat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 acetyl-CoA = CoA + acetoacetyl-CoA.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Acyl-thioester intermediateBy similarity1
Active sitei353Proton acceptorPROSITE-ProRule annotation1
Active sitei383Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-CoA acetyltransferase, cytosolic (EC:2.3.1.9)
Alternative name(s):
Cytosolic acetoacetyl-CoA thiolase
Gene namesi
Name:Acat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:87871. Acat2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002064101 – 397Acetyl-CoA acetyltransferase, cytosolicAdd BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei200N6-acetyllysineBy similarity1
Modified residuei233N6-acetyllysineBy similarity1
Modified residuei235N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8CAY6.
MaxQBiQ8CAY6.
PaxDbiQ8CAY6.
PeptideAtlasiQ8CAY6.
PRIDEiQ8CAY6.

2D gel databases

REPRODUCTION-2DPAGEQ8CAY6.

PTM databases

iPTMnetiQ8CAY6.
PhosphoSitePlusiQ8CAY6.
SwissPalmiQ8CAY6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023832.
CleanExiMM_ACAT2.
ExpressionAtlasiQ8CAY6. baseline and differential.
GenevisibleiQ8CAY6. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ8CAY6. 2 interactors.
MINTiMINT-1864806.
STRINGi10090.ENSMUSP00000007005.

Structurei

3D structure databases

ProteinModelPortaliQ8CAY6.
SMRiQ8CAY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Phylogenomic databases

eggNOGiKOG1391. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00390000009412.
HOGENOMiHOG000012238.
HOVERGENiHBG003112.
InParanoidiQ8CAY6.
KOiK00626.
OMAiESTEKHN.
OrthoDBiEOG091G09C6.
PhylomeDBiQ8CAY6.
TreeFamiTF300650.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CAY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAGSDPVVI VSAARTAIGS FNGALSTVPV HEMGTTVIKE VLQRAKVAPE
60 70 80 90 100
EVSEVIFGHV LTAGCGQNPT RQASVGAGIP YSVPAWSCQM ICGSGLKAVC
110 120 130 140 150
LAAQSIAMGD STIVVAGGME NMSKAPHLTH LRTGVRMGEV PLADSILCDG
160 170 180 190 200
LTDAFHNYHM GITAENVAKK WQVSREAQDK VAVLSQNRAE HAQKAGHFDK
210 220 230 240 250
EIVPVLVSSR KGLTEVKIDE FPRHGSNLEA MGKLKPYFLT DGTGTVTPAN
260 270 280 290 300
ASGMNDGAAA VVLMKKTEAE RRMLKPLARI VSWSQAGVEP SVMGVGPIPA
310 320 330 340 350
IKQAVAKAGW SLEDVDLFEI NEAFAAVSAA IAKELGLNPE KVNIDGGAIA
360 370 380 390
LGHPLGASGC RILVTLLHTL ERVGGTRGVA ALCIGGGMGV AMCVQRG
Length:397
Mass (Da):41,298
Last modified:September 13, 2004 - v2
Checksum:iAABC2DF22F4EE930
GO

Sequence cautioni

The sequence BAB28763 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106I → L in AAH12496 (PubMed:15489334).Curated1
Sequence conflicti115 – 119VAGGM → CLTGK (PubMed:2055458).Curated5
Sequence conflicti125A → P in AAA76575 (PubMed:2055458).Curated1
Sequence conflicti191H → T in AAA76575 (PubMed:2055458).Curated1
Sequence conflicti230A → D in BAB28763 (PubMed:16141072).Curated1
Sequence conflicti233K → T in AAA76575 (PubMed:2055458).Curated1
Sequence conflicti262V → L in BAB28763 (PubMed:16141072).Curated1
Sequence conflicti266K → E in BAC29776 (PubMed:16141072).Curated1
Sequence conflicti340E → G in AAA76575 (PubMed:2055458).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013275 mRNA. Translation: BAB28763.1. Different initiation.
AK037249 mRNA. Translation: BAC29776.1.
AK166813 mRNA. Translation: BAE39040.1.
AK167234 mRNA. Translation: BAE39358.1.
BC004823 mRNA. Translation: AAH04823.2.
BC012496 mRNA. Translation: AAH12496.2.
M35797 mRNA. Translation: AAA76575.1.
CCDSiCCDS37437.1.
PIRiA53174.
RefSeqiNP_033364.2. NM_009338.3.
UniGeneiMm.439711.

Genome annotation databases

EnsembliENSMUST00000007005; ENSMUSP00000007005; ENSMUSG00000023832.
GeneIDi110460.
KEGGimmu:110460.
UCSCiuc008alp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013275 mRNA. Translation: BAB28763.1. Different initiation.
AK037249 mRNA. Translation: BAC29776.1.
AK166813 mRNA. Translation: BAE39040.1.
AK167234 mRNA. Translation: BAE39358.1.
BC004823 mRNA. Translation: AAH04823.2.
BC012496 mRNA. Translation: AAH12496.2.
M35797 mRNA. Translation: AAA76575.1.
CCDSiCCDS37437.1.
PIRiA53174.
RefSeqiNP_033364.2. NM_009338.3.
UniGeneiMm.439711.

3D structure databases

ProteinModelPortaliQ8CAY6.
SMRiQ8CAY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CAY6. 2 interactors.
MINTiMINT-1864806.
STRINGi10090.ENSMUSP00000007005.

PTM databases

iPTMnetiQ8CAY6.
PhosphoSitePlusiQ8CAY6.
SwissPalmiQ8CAY6.

2D gel databases

REPRODUCTION-2DPAGEQ8CAY6.

Proteomic databases

EPDiQ8CAY6.
MaxQBiQ8CAY6.
PaxDbiQ8CAY6.
PeptideAtlasiQ8CAY6.
PRIDEiQ8CAY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007005; ENSMUSP00000007005; ENSMUSG00000023832.
GeneIDi110460.
KEGGimmu:110460.
UCSCiuc008alp.1. mouse.

Organism-specific databases

CTDi39.
MGIiMGI:87871. Acat2.

Phylogenomic databases

eggNOGiKOG1391. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00390000009412.
HOGENOMiHOG000012238.
HOVERGENiHBG003112.
InParanoidiQ8CAY6.
KOiK00626.
OMAiESTEKHN.
OrthoDBiEOG091G09C6.
PhylomeDBiQ8CAY6.
TreeFamiTF300650.

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiQ8CAY6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023832.
CleanExiMM_ACAT2.
ExpressionAtlasiQ8CAY6. baseline and differential.
GenevisibleiQ8CAY6. MM.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHIC_MOUSE
AccessioniPrimary (citable) accession number: Q8CAY6
Secondary accession number(s): Q3TJY7
, Q62292, Q96EB7, Q99K88, Q9CYV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.