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Protein

MICOS complex subunit Mic60

Gene

Immt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.By similarity

GO - Molecular functioni

GO - Biological processi

  • cristae formation Source: MGI
  • mitochondrial calcium ion homeostasis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
MICOS complex subunit Mic60
Alternative name(s):
Mitochondrial inner membrane protein
Mitofilin
Gene namesi
Name:Immt
Synonyms:Mic60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1923864. Immt.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 4512Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei46 – 6520HelicalSequence analysisAdd
BLAST
Topological domaini66 – 757692Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • membrane Source: MGI
  • MICOS complex Source: MGI
  • mitochondrial inner membrane Source: UniProtKB
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333MitochondrionSequence analysisAdd
BLAST
Chaini34 – 757724MICOS complex subunit Mic60PRO_0000084185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei112 – 1121PhosphoserineBy similarity
Modified residuei113 – 1131PhosphoserineBy similarity
Modified residuei210 – 2101N6-acetyllysineBy similarity
Modified residuei221 – 2211N6-acetyllysineBy similarity
Modified residuei222 – 2221PhosphoserineBy similarity
Modified residuei387 – 3871PhosphoserineBy similarity
Modified residuei389 – 3891PhosphoserineBy similarity
Modified residuei450 – 4501N6-acetyllysineBy similarity
Modified residuei506 – 5061PhosphotyrosineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CAQ8.
MaxQBiQ8CAQ8.
PaxDbiQ8CAQ8.
PeptideAtlasiQ8CAQ8.
PRIDEiQ8CAQ8.

2D gel databases

REPRODUCTION-2DPAGEQ8CAQ8.

PTM databases

iPTMnetiQ8CAQ8.
PhosphoSiteiQ8CAQ8.
SwissPalmiQ8CAQ8.

Expressioni

Gene expression databases

BgeeiQ8CAQ8.
CleanExiMM_IMMT.
ExpressionAtlasiQ8CAQ8. baseline and differential.
GenevisibleiQ8CAQ8. MM.

Interactioni

Subunit structurei

Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1, MTX2 and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. Found in a complex with CHCHD3/MIC19 and SAMM50 termed mitochondrial intermembrane space bridging (MIB) complex. Interacts with QIL1/MIC13, MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, SAMM50 and TMEM11. Interacts with APOO/MIC23/MIC26 and APOOL/MIC27 (By similarity). Interacts with HSPA1A/HSPA1B and OPA1, preferentially with the soluble OPA1 form.By similarity1 Publication

Protein-protein interaction databases

BioGridi218208. 28 interactions.
DIPiDIP-32050N.
IntActiQ8CAQ8. 33 interactions.
MINTiMINT-1860415.
STRINGi10090.ENSMUSP00000066181.

Structurei

3D structure databases

ProteinModelPortaliQ8CAQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili199 – 23133Sequence analysisAdd
BLAST
Coiled coili279 – 31739Sequence analysisAdd
BLAST
Coiled coili397 – 44246Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the MICOS complex subunit Mic60 family.Curated

Keywords - Domaini

Coiled coil, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1854. Eukaryota.
ENOG410Y49V. LUCA.
GeneTreeiENSGT00390000002313.
HOVERGENiHBG039233.
InParanoidiQ8CAQ8.
KOiK17785.
OMAiARNYFID.
OrthoDBiEOG744TB6.
PhylomeDBiQ8CAQ8.
TreeFamiTF312832.

Family and domain databases

InterProiIPR019133. Mt-IM_prot_Mitofilin.
[Graphical view]
PfamiPF09731. Mitofilin. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CAQ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRACQLSGV TVAAQSCLCG KFVLRPLRPC RRYSTSSSSG LTAGKIAGAG
60 70 80 90 100
LLFVGGGIGG TILYAKWDSH FRESVEKTIP YSDKLFGMVL GSAPYTVPLP
110 120 130 140 150
KKPVQSGPLK ISSVSEVMKD SKLPVAQSQK TKGDTPASAA STGAAQIISA
160 170 180 190 200
AGDTLSVPAP AVQHEDTIKT ECPNTNEGKS TSETTEEAFS SSVRERPPEE
210 220 230 240 250
VAARLAQQEK QEQVEMESLA KSLEDALNRT SSVTLQTITA QNAAVQAVKA
260 270 280 290 300
HSNILKTAMD NSEIAGEKKS AQWRTVEGAL KERRKAVDEA ADALLKAKEE
310 320 330 340 350
LEKMKTIIED AKKREIAGAT PHITAAEGRL HNMIVDLDNV VKKVQAAQSE
360 370 380 390 400
AKVVSQYHEL VVQARDDFRK ELDSITPDIT PGWKGMSISD LAGKLSTDDL
410 420 430 440 450
NSLIAHAHRR IDQLNRELAQ QKATEKQHIE LALEKHKLEE KRTFDSAVAK
460 470 480 490 500
ALEHHRSEIQ AEQDRKVEEV RDAMENEMRT QLRRQAAAHT DHLRDVLKVQ
510 520 530 540 550
EQELKYEFEQ GLSEKLSEQE LEFRRRSQEQ MDSFTLDINT AYARLRGIEQ
560 570 580 590 600
AVQSHAVAEE EARKAHQLWL SVEALKYSMK TSSAEMPTIP LGSAVEAIRV
610 620 630 640 650
NCSDNEFTQA LTAAIPPESL TRGVYSEETL RARFYAVQKL ARRVAMIDET
660 670 680 690 700
RNSLYQYFLS YLQSLLLFPP KQLKPPAELY PEDINTFKLL SYASYCIEHG
710 720 730 740 750
DLELAAKFVN QLKGESRRVA QDWLKEARMT LETKQIVEIL TAYASAVGIG

TTQVQQE
Length:757
Mass (Da):83,900
Last modified:March 1, 2003 - v1
Checksum:i5EB37BCA054BD7A6
GO
Isoform 2 (identifier: Q8CAQ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-151: Missing.

Show »
Length:746
Mass (Da):82,929
Checksum:iD7163AC815B9D0D0
GO
Isoform 3 (identifier: Q8CAQ8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-151: Missing.
     186-218: EEAFSSSVRERPPEEVAARLAQQEKQEQVEMES → A
     387-392: SISDLA → T

Note: No experimental confirmation available.
Show »
Length:709
Mass (Da):78,727
Checksum:iE5E8EC4154F1853D
GO
Isoform 4 (identifier: Q8CAQ8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-151: Missing.
     186-194: EEAFSSSVR → GSLIIYIML
     195-757: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:183
Mass (Da):19,194
Checksum:i67A485D7DF8DA8D4
GO
Isoform 5 (identifier: Q8CAQ8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     757-757: E → FWIKAVFKSQMSNPTSFKVTRLNSVQKNRSSPLI

Note: No experimental confirmation available.
Show »
Length:790
Mass (Da):87,694
Checksum:i85B4294F1E2C734E
GO

Sequence cautioni

The sequence BAB24817.1 differs from that shown. Reason: Frameshift at position 754. Curated
The sequence BAB24817.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti532 – 5321D → E in BAB24817 (PubMed:16141072).Curated
Sequence conflicti658 – 6581F → L in BAB24817 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei141 – 15111Missing in isoform 2, isoform 3 and isoform 4. 1 PublicationVSP_007003Add
BLAST
Alternative sequencei186 – 21833EEAFS…VEMES → A in isoform 3. 1 PublicationVSP_007004Add
BLAST
Alternative sequencei186 – 1949EEAFSSSVR → GSLIIYIML in isoform 4. 1 PublicationVSP_013222
Alternative sequencei195 – 757563Missing in isoform 4. 1 PublicationVSP_013223Add
BLAST
Alternative sequencei387 – 3926SISDLA → T in isoform 3. 1 PublicationVSP_007005
Alternative sequencei757 – 7571E → FWIKAVFKSQMSNPTSFKVT RLNSVQKNRSSPLI in isoform 5. 1 PublicationVSP_013224

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006977 mRNA. Translation: BAB24817.1. Sequence problems.
AK030841 mRNA. Translation: BAC27154.1.
AK033126 mRNA. Translation: BAC28163.1.
AK038129 mRNA. Translation: BAC29936.1.
AK049189 mRNA. Translation: BAC33599.1.
BC055840 mRNA. Translation: AAH55840.1.
BC080790 mRNA. Translation: AAH80790.1.
CCDSiCCDS39509.1. [Q8CAQ8-1]
CCDS57425.1. [Q8CAQ8-2]
CCDS57426.1. [Q8CAQ8-3]
RefSeqiNP_001240610.1. NM_001253681.1. [Q8CAQ8-2]
NP_001240616.1. NM_001253687.1. [Q8CAQ8-3]
NP_001240617.1. NM_001253688.1.
NP_083949.2. NM_029673.3. [Q8CAQ8-1]
UniGeneiMm.235123.
Mm.490578.

Genome annotation databases

EnsembliENSMUST00000064062; ENSMUSP00000066181; ENSMUSG00000052337. [Q8CAQ8-1]
ENSMUST00000101301; ENSMUSP00000098859; ENSMUSG00000052337. [Q8CAQ8-2]
ENSMUST00000114151; ENSMUSP00000109788; ENSMUSG00000052337. [Q8CAQ8-3]
GeneIDi76614.
KEGGimmu:76614.
UCSCiuc009che.2. mouse. [Q8CAQ8-4]
uc009chi.2. mouse. [Q8CAQ8-1]
uc009chj.2. mouse. [Q8CAQ8-2]
uc009chm.2. mouse. [Q8CAQ8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006977 mRNA. Translation: BAB24817.1. Sequence problems.
AK030841 mRNA. Translation: BAC27154.1.
AK033126 mRNA. Translation: BAC28163.1.
AK038129 mRNA. Translation: BAC29936.1.
AK049189 mRNA. Translation: BAC33599.1.
BC055840 mRNA. Translation: AAH55840.1.
BC080790 mRNA. Translation: AAH80790.1.
CCDSiCCDS39509.1. [Q8CAQ8-1]
CCDS57425.1. [Q8CAQ8-2]
CCDS57426.1. [Q8CAQ8-3]
RefSeqiNP_001240610.1. NM_001253681.1. [Q8CAQ8-2]
NP_001240616.1. NM_001253687.1. [Q8CAQ8-3]
NP_001240617.1. NM_001253688.1.
NP_083949.2. NM_029673.3. [Q8CAQ8-1]
UniGeneiMm.235123.
Mm.490578.

3D structure databases

ProteinModelPortaliQ8CAQ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218208. 28 interactions.
DIPiDIP-32050N.
IntActiQ8CAQ8. 33 interactions.
MINTiMINT-1860415.
STRINGi10090.ENSMUSP00000066181.

PTM databases

iPTMnetiQ8CAQ8.
PhosphoSiteiQ8CAQ8.
SwissPalmiQ8CAQ8.

2D gel databases

REPRODUCTION-2DPAGEQ8CAQ8.

Proteomic databases

EPDiQ8CAQ8.
MaxQBiQ8CAQ8.
PaxDbiQ8CAQ8.
PeptideAtlasiQ8CAQ8.
PRIDEiQ8CAQ8.

Protocols and materials databases

DNASUi76614.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064062; ENSMUSP00000066181; ENSMUSG00000052337. [Q8CAQ8-1]
ENSMUST00000101301; ENSMUSP00000098859; ENSMUSG00000052337. [Q8CAQ8-2]
ENSMUST00000114151; ENSMUSP00000109788; ENSMUSG00000052337. [Q8CAQ8-3]
GeneIDi76614.
KEGGimmu:76614.
UCSCiuc009che.2. mouse. [Q8CAQ8-4]
uc009chi.2. mouse. [Q8CAQ8-1]
uc009chj.2. mouse. [Q8CAQ8-2]
uc009chm.2. mouse. [Q8CAQ8-3]

Organism-specific databases

CTDi10989.
MGIiMGI:1923864. Immt.

Phylogenomic databases

eggNOGiKOG1854. Eukaryota.
ENOG410Y49V. LUCA.
GeneTreeiENSGT00390000002313.
HOVERGENiHBG039233.
InParanoidiQ8CAQ8.
KOiK17785.
OMAiARNYFID.
OrthoDBiEOG744TB6.
PhylomeDBiQ8CAQ8.
TreeFamiTF312832.

Miscellaneous databases

ChiTaRSiImmt. mouse.
PROiQ8CAQ8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CAQ8.
CleanExiMM_IMMT.
ExpressionAtlasiQ8CAQ8. baseline and differential.
GenevisibleiQ8CAQ8. MM.

Family and domain databases

InterProiIPR019133. Mt-IM_prot_Mitofilin.
[Graphical view]
PfamiPF09731. Mitofilin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 503-757 (ISOFORM 5).
    Strain: C57BL/6J.
    Tissue: Testis and Thymus.
  2. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 353-365; 395-409; 581-599 AND 672-688, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain and Hippocampus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 475-757.
    Strain: Czech II and FVB/N.
    Tissue: Kidney and Mammary tumor.
  4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-506, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. "ChChd3, an inner mitochondrial membrane protein, is essential for maintaining crista integrity and mitochondrial function."
    Darshi M., Mendiola V.L., Mackey M.R., Murphy A.N., Koller A., Perkins G.A., Ellisman M.H., Taylor S.S.
    J. Biol. Chem. 286:2918-2932(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHCHD3 AND OPA1.

Entry informationi

Entry nameiMIC60_MOUSE
AccessioniPrimary (citable) accession number: Q8CAQ8
Secondary accession number(s): Q66JS4
, Q7TNE2, Q8C7V1, Q8CCI0, Q8CDA8, Q9D9F6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.