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Q8C9W3

- ATS2_MOUSE

UniProt

Q8C9W3 - ATS2_MOUSE

Protein

A disintegrin and metalloproteinase with thrombospondin motifs 2

Gene

Adamts2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (20 Jun 2003)
      Previous versions | rss
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    Functioni

    Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis By similarity.By similarity

    Catalytic activityi

    Cleaves the N-propeptide of collagen chain alpha-1(I) at Pro-|-Gln and of alpha-1(II) and alpha-2(I) at Ala-|-Gln.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi409 – 4091Zinc; catalyticPROSITE-ProRule annotation
    Active sitei410 – 4101PROSITE-ProRule annotation
    Metal bindingi413 – 4131Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi419 – 4191Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. peptidase activity Source: MGI
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. collagen catabolic process Source: UniProtKB-KW
    2. collagen fibril organization Source: MGI
    3. lung development Source: MGI
    4. protein processing Source: MGI
    5. skin development Source: MGI
    6. spermatogenesis Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Biological processi

    Collagen degradation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.

    Protein family/group databases

    MEROPSiM12.301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 2 (EC:3.4.24.14)
    Short name:
    ADAM-TS 2
    Short name:
    ADAM-TS2
    Short name:
    ADAMTS-2
    Alternative name(s):
    Procollagen I N-proteinase
    Short name:
    PC I-NP
    Procollagen I/II amino propeptide-processing enzyme
    Procollagen N-endopeptidase
    Short name:
    pNPI
    Gene namesi
    Name:Adamts2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1347356. Adamts2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular matrix Source: MGI
    2. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Propeptidei29 – 260232By similarityPRO_0000029161Add
    BLAST
    Chaini261 – 1213953A disintegrin and metalloproteinase with thrombospondin motifs 2PRO_0000029160Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi344 ↔ 393By similarity
    Disulfide bondi387 ↔ 466By similarity
    Disulfide bondi426 ↔ 452By similarity
    Disulfide bondi493 ↔ 518By similarity
    Disulfide bondi504 ↔ 527By similarity
    Disulfide bondi513 ↔ 546By similarity
    Disulfide bondi540 ↔ 551By similarity
    Disulfide bondi574 ↔ 611By similarity
    Disulfide bondi578 ↔ 616By similarity
    Disulfide bondi589 ↔ 601By similarity
    Glycosylationi949 – 9491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi950 – 9501N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi988 ↔ 1024By similarity
    Disulfide bondi992 ↔ 1029By similarity
    Glycosylationi994 – 9941N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1003 ↔ 1013By similarity
    Glycosylationi1032 – 10321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1099 – 10991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1147 – 11471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1152 – 11521N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The precursor is cleaved by a furin endopeptidase.By similarity
    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ8C9W3.
    PRIDEiQ8C9W3.

    PTM databases

    PhosphoSiteiQ8C9W3.

    Expressioni

    Gene expression databases

    BgeeiQ8C9W3.
    GenevestigatoriQ8C9W3.

    Interactioni

    Subunit structurei

    May belong to a multimeric complex. Binds specifically to collagen type XIV By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C9W3.
    SMRiQ8C9W3. Positions 269-786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini267 – 471205Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini480 – 56081DisintegrinAdd
    BLAST
    Domaini561 – 61757TSP type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini855 – 91359TSP type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini915 – 97561TSP type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini976 – 103055TSP type-1 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1060 – 109839PLACPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni723 – 851129SpacerAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi692 – 6943Cell attachment siteSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi618 – 722105Cys-richAdd
    BLAST

    Domaini

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

    Sequence similaritiesi

    Contains 1 disintegrin domain.Curated
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
    Contains 1 PLAC domain.PROSITE-ProRule annotation
    Contains 4 TSP type-1 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG307411.
    GeneTreeiENSGT00740000114846.
    HOGENOMiHOG000034222.
    HOVERGENiHBG004314.
    InParanoidiQ8C9W3.
    KOiK08618.
    OMAiIHEDSLN.
    OrthoDBiEOG76HQ0N.
    PhylomeDBiQ8C9W3.
    TreeFamiTF313537.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    InterProiIPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR013275. Pept_M12B_ADAM-TS2.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR002870. Peptidase_M12B_N.
    IPR010909. PLAC.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view]
    PfamiPF05986. ADAM_spacer1. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 4 hits.
    [Graphical view]
    PRINTSiPR01859. ADAMTS2.
    PR01857. ADAMTSFAMILY.
    SMARTiSM00209. TSP1. 4 hits.
    [Graphical view]
    SUPFAMiSSF82895. SSF82895. 4 hits.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS50900. PLAC. 1 hit.
    PS50092. TSP1. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8C9W3-1 [UniParc]FASTAAdd to Basket

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    MDPPAGAARR LLCPALLLLL LPPPPLLLLP PPPASVRLVA ATEPPGGPPG     50
    QGAERILAVP VRTDAQGRLV SHVVSLETAG AGVRARRAAL DQTSGLPGGA 100
    AQDPGGRLFY NLTVFGRDLH LRLRPNARLV APGATVEWQG ETGDTRVEPL 150
    LGSCLYVGDV ADLPKASSVA LSNCDGLAGL IRMEEEEFFI EPLEKGQTDQ 200
    EAEQGRVHVV YRRPPTPKPP PVSEPQALDT GVSQGNLDSL SRALGVLEER 250
    INSSRRRVRR HATDDDYNIE VLLGVDDSVV QFHGKEHVQK YLLTLMNIVN 300
    EIYHDESLGA HINVVLVRII LLSHAKSMSL IEIGNPSQSL ENVCRWAYLQ 350
    QKPDTDHDEY HDHAIFLTRQ DFGPSGMQGY APVTGMCHPV RSCTLNHEDG 400
    FSSAFVVAHE TGHVLGMEHD GQGNRCGDEV RLGSIMAPLV QAAFHRFHWS 450
    RCSQQELSRY LHSYDCLRDD PFAHDWPALP QLPGLHYSMN EQCRFDFGLG 500
    YMMCTAFRTF DPCKQLWCSH PDNPYFCKTK KGPPLDGTMC APGKHCFKGH 550
    CIWLTPDILK RDGNWGAWTP FGSCSRTCGT GVKFRTRQCD NPHPANGGRT 600
    CSGLAYDFQL CNPQDCPNSL ADFREEQCQQ WDLYFEHGDV QHHWLPHEHR 650
    DAKERCHLYC ESKETGEVVS MKRMVHDGTR CSYKDAFSLC VRGDCRKVGC 700
    DGVIGSRKQE DKCGVCGGDN THCKVVKGTF TRSPRKQDYI KMFEIPAGAR 750
    HLLIQEADTT SHHLSVKNLE TGKFILNEEN HLDPNSRSFI AMGVEWEYRN 800
    EDERETLQTI GPLHGTITVL VIPEGDTRIS LTYKYMIHED SLNVDDNNVL 850
    EDDAVRHEWA LKKWSPCSKP CGGGSQFTKY GCRRRLDSKM VHRAFCSALA 900
    KPKAIRRACN PQECSQPVWV TGEWEPCTQS CGRTGMQVRS VRCIQPLHNN 950
    TTRSVHTKHC NDHRPESRRA CNRELCPGRW RAGSWSQCSV TCGNGTQERP 1000
    VLCRTADDNF GVCREERPET ARICRLAPCP RNGSDPSKKS YVVQWLSRPD 1050
    PDSPIQKISS KDQCQGDKSM FCRMEVLSRY CSIPSYNKLC CKSCNPPRNL 1100
    SNTEDGGVEP PPGKHNDIDV FMPTLPGPTV ATQVQPSPGP PLEAPLNVSS 1150
    TNATEDHPET NAVDVPYKIH GVDEEVPSPN LIPRRPSLYV KTRNQRIQEL 1200
    INAVQRKEKP GKF 1213
    Length:1,213
    Mass (Da):135,299
    Last modified:June 20, 2003 - v2
    Checksum:iB27431E00443EDB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK040370 mRNA. Translation: BAC30572.1.
    BC046456 mRNA. Translation: AAH46456.1.
    CCDSiCCDS24636.1.
    RefSeqiNP_783574.1. NM_175643.3.
    UniGeneiMm.110597.
    Mm.339048.

    Genome annotation databases

    EnsembliENSMUST00000040523; ENSMUSP00000040171; ENSMUSG00000036545.
    GeneIDi216725.
    KEGGimmu:216725.
    UCSCiuc007iso.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK040370 mRNA. Translation: BAC30572.1 .
    BC046456 mRNA. Translation: AAH46456.1 .
    CCDSi CCDS24636.1.
    RefSeqi NP_783574.1. NM_175643.3.
    UniGenei Mm.110597.
    Mm.339048.

    3D structure databases

    ProteinModelPortali Q8C9W3.
    SMRi Q8C9W3. Positions 269-786.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.301.

    PTM databases

    PhosphoSitei Q8C9W3.

    Proteomic databases

    PaxDbi Q8C9W3.
    PRIDEi Q8C9W3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040523 ; ENSMUSP00000040171 ; ENSMUSG00000036545 .
    GeneIDi 216725.
    KEGGi mmu:216725.
    UCSCi uc007iso.1. mouse.

    Organism-specific databases

    CTDi 9509.
    MGIi MGI:1347356. Adamts2.

    Phylogenomic databases

    eggNOGi NOG307411.
    GeneTreei ENSGT00740000114846.
    HOGENOMi HOG000034222.
    HOVERGENi HBG004314.
    InParanoidi Q8C9W3.
    KOi K08618.
    OMAi IHEDSLN.
    OrthoDBi EOG76HQ0N.
    PhylomeDBi Q8C9W3.
    TreeFami TF313537.

    Enzyme and pathway databases

    Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.

    Miscellaneous databases

    NextBioi 375268.
    PROi Q8C9W3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8C9W3.
    Genevestigatori Q8C9W3.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    InterProi IPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR013275. Pept_M12B_ADAM-TS2.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR002870. Peptidase_M12B_N.
    IPR010909. PLAC.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view ]
    Pfami PF05986. ADAM_spacer1. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 4 hits.
    [Graphical view ]
    PRINTSi PR01859. ADAMTS2.
    PR01857. ADAMTSFAMILY.
    SMARTi SM00209. TSP1. 4 hits.
    [Graphical view ]
    SUPFAMi SSF82895. SSF82895. 4 hits.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS50900. PLAC. 1 hit.
    PS50092. TSP1. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1058-1068, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiATS2_MOUSE
    AccessioniPrimary (citable) accession number: Q8C9W3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: June 20, 2003
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3