Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Liprin-beta-1

Gene

Ppfibp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Liprin-beta-1
Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1
Short name:
PTPRF-interacting protein-binding protein 1
Gene namesi
Name:Ppfibp1
Synonyms:Kiaa1230
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1914783. Ppfibp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Liprin-beta-1PRO_0000191035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei39 – 391PhosphothreonineBy similarity
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei291 – 2911N6-acetyllysineBy similarity
Modified residuei403 – 4031PhosphoserineCombined sources
Modified residuei435 – 4351PhosphoserineBy similarity
Modified residuei500 – 5001PhosphoserineBy similarity
Modified residuei538 – 5381PhosphoserineCombined sources
Modified residuei560 – 5601PhosphoserineCombined sources
Modified residuei595 – 5951PhosphoserineCombined sources
Modified residuei753 – 7531PhosphoserineCombined sources
Modified residuei757 – 7571PhosphoserineCombined sources
Modified residuei957 – 9571PhosphoserineBy similarity
Modified residuei959 – 9591PhosphoserineBy similarity
Modified residuei961 – 9611PhosphoserineCombined sources
Modified residuei963 – 9631PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C8U0.
MaxQBiQ8C8U0.
PaxDbiQ8C8U0.
PRIDEiQ8C8U0.

PTM databases

iPTMnetiQ8C8U0.
PhosphoSiteiQ8C8U0.

Expressioni

Gene expression databases

BgeeiQ8C8U0.
ExpressionAtlasiQ8C8U0. baseline and differential.
GenevisibleiQ8C8U0. MM.

Interactioni

Subunit structurei

Forms homodimers and heterodimers. Interacts with S100A4 in a calcium-dependent mode (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8C8U0. 2 interactions.
MINTiMINT-4100442.
STRINGi10090.ENSMUSP00000107250.

Structurei

Secondary structure

1
969
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi603 – 6053Combined sources
Helixi608 – 61710Combined sources
Turni618 – 6203Combined sources
Helixi624 – 6274Combined sources
Turni628 – 6303Combined sources
Helixi635 – 6395Combined sources
Helixi642 – 6487Combined sources
Helixi654 – 66916Combined sources
Helixi675 – 6773Combined sources
Helixi680 – 69011Combined sources
Helixi693 – 6953Combined sources
Helixi696 – 7027Combined sources
Helixi706 – 7105Combined sources
Helixi714 – 7196Combined sources
Helixi725 – 74016Combined sources
Turni741 – 7433Combined sources
Beta strandi747 – 7493Combined sources
Helixi755 – 7573Combined sources
Helixi760 – 7656Combined sources
Helixi768 – 77710Combined sources
Turni781 – 7833Combined sources
Helixi784 – 7874Combined sources
Helixi794 – 7996Combined sources
Helixi805 – 8117Combined sources
Helixi819 – 83315Combined sources
Helixi835 – 84410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TADX-ray2.90C/D593-853[»]
ProteinModelPortaliQ8C8U0.
SMRiQ8C8U0. Positions 194-256, 601-853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini606 – 67065SAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini678 – 74164SAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini763 – 83573SAM 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili99 – 310212Sequence analysisAdd
BLAST

Domaini

The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type beta/beta. The C-terminal, non-coiled coil regions mediate heterodimerization type beta/alpha and interaction with S100A4 (By similarity).By similarity

Sequence similaritiesi

Belongs to the liprin family. Liprin-beta subfamily.Curated
Contains 3 SAM (sterile alpha motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1899. Eukaryota.
ENOG410XSFF. LUCA.
GeneTreeiENSGT00760000119138.
HOGENOMiHOG000082535.
HOVERGENiHBG052331.
InParanoidiQ8C8U0.
OMAiVPEEFHT.
OrthoDBiEOG71G9T5.
PhylomeDBiQ8C8U0.
TreeFamiTF314207.

Family and domain databases

Gene3Di1.10.150.50. 3 hits.
InterProiIPR029515. Liprin.
IPR030437. PPFIBP1.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 1 hit.
PTHR12587:SF16. PTHR12587:SF16. 1 hit.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C8U0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSDASDMLA AALEQMDGII AGSKALEYSN GIFDCQSPTS PFMGSLRALH
60 70 80 90 100
LVEDLRGLLE MMETDEKEGL RCQIPDSTAE VLIEWLQNQM TNGHLPGNGD
110 120 130 140 150
VYQERLARLE NDKESLVLQV SVLTDQVEAQ GEKIRDLEFC LEEHREKLNA
160 170 180 190 200
TEEMLQQELL SRTSLETQKL ELMAEISNLK LKLTAVEKDR LDYEDRFRDT
210 220 230 240 250
EGLIQEINDL RLKVNEMDGE RLQYEKKLKS TKDELASLKE QLEEKECEVK
260 270 280 290 300
RLQERLVCKA KGEGIEVLDR DIEVQKMKKA VESLMAANEE KERKIEDLRQ
310 320 330 340 350
CLSRYRKMQD PAVLAQGQDS ECEGLFHSSS ISTLLDAQGF SDLERSTSST
360 370 380 390 400
PGMGSPSRDL LHTSAPEEFH TSVLQASIPS LLPPSVDVDT CEKPKLPTKP
410 420 430 440 450
ETSFEEGDGR AILGAAAEVS LSDGVSTSSL QKSSSLGNLK KEASDGTDKA
460 470 480 490 500
PTDSRTFGTL PPKVPGHEAS VDDNPFGTRK ARSSFGRGFF KIKSGKRTAS
510 520 530 540 550
APNLAETEKE TAEHLNLAGT SRSKGSQGTS PFPMSPPSPD SRKKSRGIMR
560 570 580 590 600
LFGKLRRSQS TTFNPDDMSE PEFKRGGTRA TAGPRLGWSR DLGQSNSDLD
610 620 630 640 650
MPFAKWTKEQ VCSWLAEQGL GSYLSSGKHW IISGQTLLQA SQQDLEKELG
660 670 680 690 700
IKHSLHRKKL QLALQALGSE EETNYGKLDF NWVTRWLDDI GLPQYKTQFD
710 720 730 740 750
EGRVDGRMLH YMTVDDLLSL KVVSVLHHLS IKRAIQVLRI NNFEPNCLRR
760 770 780 790 800
RPSDENSITP SEVQQWTNHR VMEWLRSVDL AEYAPNLRGS GVHGGLMVLE
810 820 830 840 850
PRFNVETMAQ LLNIPPNKTL LRRHLATHFN LLIGAEAQHQ KRDAMELPDY
860 870 880 890 900
VLLTATAKVK PKKLTFSNFG NLRKKKHEDG EEYVCPMELG QASGSSQKGF
910 920 930 940 950
RPGLDMRLYE EDDLDRLEQM EDSEGTVRQI GAFSEGINNL THMLKEDDMF
960
KDFAARSPSA SITDEDSNV
Note: No experimental confirmation available.
Length:969
Mass (Da):108,540
Last modified:December 7, 2004 - v3
Checksum:i7322E25285DDF499
GO
Isoform 2 (identifier: Q8C8U0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-276: Missing.

Note: No experimental confirmation available.
Show »
Length:693
Mass (Da):76,921
Checksum:iDA531714968AEC25
GO
Isoform 3 (identifier: Q8C8U0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     271-271: D → DENIKKKLKEKN

Note: No experimental confirmation available.
Show »
Length:980
Mass (Da):109,894
Checksum:i867A8633F7167EAA
GO

Sequence cautioni

The sequence BAD32411.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti360 – 3601L → P in BAD32411 (PubMed:15489334).Curated
Sequence conflicti389 – 3891D → E (PubMed:16141072).Curated
Sequence conflicti389 – 3891D → E (PubMed:15489334).Curated
Sequence conflicti817 – 87458NKTLL…GNLRK → CQQHLHLPLTVLFLYIFLNT CIYTVYRKQNLYFVTQGSKD HIKMKKPQYFNDHIPADS in BAC31950 (PubMed:16141072).CuratedAdd
BLAST
Sequence conflicti875 – 96995Missing (PubMed:16141072).CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 276276Missing in isoform 2. 1 PublicationVSP_012095Add
BLAST
Alternative sequencei271 – 2711D → DENIKKKLKEKN in isoform 3. 1 PublicationVSP_012096

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044496 mRNA. Translation: BAC31950.1.
AK014559 mRNA. Translation: BAB29428.1.
AK173133 mRNA. Translation: BAD32411.1. Different initiation.
BC049862 mRNA. Translation: AAH49862.1.
BC058176 mRNA. Translation: AAH58176.1.
BC072603 mRNA. Translation: AAH72603.1.
CCDSiCCDS39715.1. [Q8C8U0-1]
CCDS51958.1. [Q8C8U0-3]
RefSeqiNP_001163904.1. NM_001170433.1. [Q8C8U0-3]
NP_080497.1. NM_026221.2. [Q8C8U0-1]
UniGeneiMm.103382.

Genome annotation databases

EnsembliENSMUST00000016631; ENSMUSP00000016631; ENSMUSG00000016487. [Q8C8U0-1]
ENSMUST00000111623; ENSMUSP00000107250; ENSMUSG00000016487. [Q8C8U0-3]
GeneIDi67533.
KEGGimmu:67533.
UCSCiuc009esm.2. mouse. [Q8C8U0-1]
uc009esn.2. mouse. [Q8C8U0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044496 mRNA. Translation: BAC31950.1.
AK014559 mRNA. Translation: BAB29428.1.
AK173133 mRNA. Translation: BAD32411.1. Different initiation.
BC049862 mRNA. Translation: AAH49862.1.
BC058176 mRNA. Translation: AAH58176.1.
BC072603 mRNA. Translation: AAH72603.1.
CCDSiCCDS39715.1. [Q8C8U0-1]
CCDS51958.1. [Q8C8U0-3]
RefSeqiNP_001163904.1. NM_001170433.1. [Q8C8U0-3]
NP_080497.1. NM_026221.2. [Q8C8U0-1]
UniGeneiMm.103382.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TADX-ray2.90C/D593-853[»]
ProteinModelPortaliQ8C8U0.
SMRiQ8C8U0. Positions 194-256, 601-853.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8C8U0. 2 interactions.
MINTiMINT-4100442.
STRINGi10090.ENSMUSP00000107250.

PTM databases

iPTMnetiQ8C8U0.
PhosphoSiteiQ8C8U0.

Proteomic databases

EPDiQ8C8U0.
MaxQBiQ8C8U0.
PaxDbiQ8C8U0.
PRIDEiQ8C8U0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016631; ENSMUSP00000016631; ENSMUSG00000016487. [Q8C8U0-1]
ENSMUST00000111623; ENSMUSP00000107250; ENSMUSG00000016487. [Q8C8U0-3]
GeneIDi67533.
KEGGimmu:67533.
UCSCiuc009esm.2. mouse. [Q8C8U0-1]
uc009esn.2. mouse. [Q8C8U0-3]

Organism-specific databases

CTDi8496.
MGIiMGI:1914783. Ppfibp1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1899. Eukaryota.
ENOG410XSFF. LUCA.
GeneTreeiENSGT00760000119138.
HOGENOMiHOG000082535.
HOVERGENiHBG052331.
InParanoidiQ8C8U0.
OMAiVPEEFHT.
OrthoDBiEOG71G9T5.
PhylomeDBiQ8C8U0.
TreeFamiTF314207.

Miscellaneous databases

PROiQ8C8U0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C8U0.
ExpressionAtlasiQ8C8U0. baseline and differential.
GenevisibleiQ8C8U0. MM.

Family and domain databases

Gene3Di1.10.150.50. 3 hits.
InterProiIPR029515. Liprin.
IPR030437. PPFIBP1.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 1 hit.
PTHR12587:SF16. PTHR12587:SF16. 1 hit.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Retina.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Fetal brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Mammary cancer.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-538 AND SER-560, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40; SER-403; SER-538; SER-560; SER-595; SER-753; SER-757 AND SER-961, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiLIPB1_MOUSE
AccessioniPrimary (citable) accession number: Q8C8U0
Secondary accession number(s): Q69ZN5
, Q6GQV3, Q80VB4, Q9CUT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.