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Q8C8N3

- VWC2_MOUSE

UniProt

Q8C8N3 - VWC2_MOUSE

Protein

Brorin

Gene

Vwc2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    BMP antagonist which may play a role in neural development. Promotes cell adhesion.2 Publications

    GO - Biological processi

    1. negative regulation of BMP signaling pathway Source: HGNC
    2. positive regulation of cell-substrate adhesion Source: MGI
    3. positive regulation of neuron differentiation Source: HGNC

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Brorin
    Alternative name(s):
    Brain-specific chordin-like protein
    CR (chordin-like cysteine-rich) domain-containing adhesive protein
    Short name:
    Cradin
    von Willebrand factor C domain-containing protein 2
    Gene namesi
    Name:Vwc2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2442987. Vwc2.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: MGI
    2. basement membrane Source: MGI
    3. cell junction Source: UniProtKB-KW
    4. extracellular matrix Source: MGI
    5. extracellular space Source: HGNC
    6. interstitial matrix Source: MGI
    7. synapse Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Basement membrane, Cell junction, Extracellular matrix, Secreted, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 324297BrorinPRO_0000307160Add
    BLAST

    Proteomic databases

    PRIDEiQ8C8N3.

    Expressioni

    Tissue specificityi

    Predominantly expressed in the brain (at protein level). It is expressed in the neurons but not the glial cells.3 Publications

    Developmental stagei

    At 12.5 dpc, predominantly expressed in the developing diencephalon. At 16.5 dpc and 18.5 dpc, expressed in the brain, spinal cord, developing neural tubes and tongue but not in the cerebral cortex. At 16.5 dpc, present in developing oral and tooth germ epithelia (at protein level).2 Publications

    Gene expression databases

    BgeeiQ8C8N3.
    CleanExiMM_VWC2.
    GenevestigatoriQ8C8N3.

    Interactioni

    Subunit structurei

    Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including VWC2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing.1 Publication

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000049692.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C8N3.
    SMRiQ8C8N3. Positions 152-211, 213-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini152 – 21160VWFC 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini215 – 27359VWFC 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi114 – 1163Mediates cell adhesion

    Sequence similaritiesi

    Contains 2 VWFC domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG83498.
    GeneTreeiENSGT00720000108792.
    HOGENOMiHOG000036086.
    HOVERGENiHBG094842.
    InParanoidiQ8C8N3.
    OMAiSKQKQAW.
    OrthoDBiEOG73Z2TT.
    PhylomeDBiQ8C8N3.
    TreeFamiTF329913.

    Family and domain databases

    InterProiIPR001007. VWF_C.
    [Graphical view]
    PfamiPF00093. VWC. 1 hit.
    [Graphical view]
    SMARTiSM00214. VWC. 2 hits.
    [Graphical view]
    PROSITEiPS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8C8N3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSSSAMAVG ALSSSLLVTC CLMVALCSPS IPLEKLAQAP EQPGQEKREH    50
    ASRDSPGRVS ELGRASRDEG SSARDWKSKG SRALSGREAW SKQKQAWAAQ 100
    GGSAKAADWQ VRPRGDTPQG EPPAAAQEAI SLELVPTPEL PEEYAYPDYR 150
    GKGCVDESGF VYAIGEKFAP GPSACPCLCT EEGPLCAQPE CPRLHPRCIH 200
    VDNSQCCPQC KEKKNYCEFR GKTYQTLEEF VVSPCERCRC EANGEVLCTV 250
    SACPQTECVD PVYEPDQCCP ICKNGPNCFA ETAVIPAGRE VKTDECTICH 300
    CTYEEGTWRI ERQAMCTRHE CRQM 324
    Length:324
    Mass (Da):35,383
    Last modified:March 1, 2003 - v1
    Checksum:iE6DD3FEB07F91DCB
    GO

    Sequence cautioni

    The sequence CAI25053.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB292670 mRNA. Translation: BAF51550.1.
    DQ421811 mRNA. Translation: ABD83334.1.
    AK044771 mRNA. Translation: BAC32080.1.
    AK141514 mRNA. Translation: BAE24710.1.
    AL663084, AL663071 Genomic DNA. Translation: CAI25053.2. Different initiation.
    AL663071, AL663084 Genomic DNA. Translation: CAI35949.1.
    CH466574 Genomic DNA. Translation: EDL40630.1.
    CH466574 Genomic DNA. Translation: EDL40631.1.
    BC120564 mRNA. Translation: AAI20565.1.
    BC137825 mRNA. Translation: AAI37826.1.
    CCDSiCCDS36111.1.
    RefSeqiNP_796007.1. NM_177033.3.
    XP_006514796.1. XM_006514733.1.
    UniGeneiMm.337851.
    Mm.487612.

    Genome annotation databases

    EnsembliENSMUST00000056344; ENSMUSP00000049692; ENSMUSG00000050830.
    ENSMUST00000109681; ENSMUSP00000105303; ENSMUSG00000050830.
    ENSMUST00000129670; ENSMUSP00000128761; ENSMUSG00000050830.
    GeneIDi319922.
    KEGGimmu:319922.
    UCSCiuc007iac.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB292670 mRNA. Translation: BAF51550.1 .
    DQ421811 mRNA. Translation: ABD83334.1 .
    AK044771 mRNA. Translation: BAC32080.1 .
    AK141514 mRNA. Translation: BAE24710.1 .
    AL663084 , AL663071 Genomic DNA. Translation: CAI25053.2 . Different initiation.
    AL663071 , AL663084 Genomic DNA. Translation: CAI35949.1 .
    CH466574 Genomic DNA. Translation: EDL40630.1 .
    CH466574 Genomic DNA. Translation: EDL40631.1 .
    BC120564 mRNA. Translation: AAI20565.1 .
    BC137825 mRNA. Translation: AAI37826.1 .
    CCDSi CCDS36111.1.
    RefSeqi NP_796007.1. NM_177033.3.
    XP_006514796.1. XM_006514733.1.
    UniGenei Mm.337851.
    Mm.487612.

    3D structure databases

    ProteinModelPortali Q8C8N3.
    SMRi Q8C8N3. Positions 152-211, 213-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000049692.

    Proteomic databases

    PRIDEi Q8C8N3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000056344 ; ENSMUSP00000049692 ; ENSMUSG00000050830 .
    ENSMUST00000109681 ; ENSMUSP00000105303 ; ENSMUSG00000050830 .
    ENSMUST00000129670 ; ENSMUSP00000128761 ; ENSMUSG00000050830 .
    GeneIDi 319922.
    KEGGi mmu:319922.
    UCSCi uc007iac.1. mouse.

    Organism-specific databases

    CTDi 375567.
    MGIi MGI:2442987. Vwc2.

    Phylogenomic databases

    eggNOGi NOG83498.
    GeneTreei ENSGT00720000108792.
    HOGENOMi HOG000036086.
    HOVERGENi HBG094842.
    InParanoidi Q8C8N3.
    OMAi SKQKQAW.
    OrthoDBi EOG73Z2TT.
    PhylomeDBi Q8C8N3.
    TreeFami TF329913.

    Miscellaneous databases

    NextBioi 395657.
    PROi Q8C8N3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8C8N3.
    CleanExi MM_VWC2.
    Genevestigatori Q8C8N3.

    Family and domain databases

    InterProi IPR001007. VWF_C.
    [Graphical view ]
    Pfami PF00093. VWC. 1 hit.
    [Graphical view ]
    SMARTi SM00214. VWC. 2 hits.
    [Graphical view ]
    PROSITEi PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Brorin, a novel secreted bone morphogenetic protein antagonist, promotes neurogenesis in mouse neural precursor cells."
      Koike N., Kassai Y., Kouta Y., Miwa H., Konishi M., Itoh N.
      J. Biol. Chem. 282:15843-15850(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. "A novel member of cysteine-rich protein family highly expressed in brain."
      Mochida Y., Yamauchi M.
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Retina and Spinal cord.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    8. "High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes."
      Schwenk J., Harmel N., Brechet A., Zolles G., Berkefeld H., Muller C.S., Bildl W., Baehrens D., Huber B., Kulik A., Klocker N., Schulte U., Fakler B.
      Neuron 74:621-633(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AMPAR COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiVWC2_MOUSE
    AccessioniPrimary (citable) accession number: Q8C8N3
    Secondary accession number(s): B2RQ98, Q5SSH5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3