Q8C8N3 (VWC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brorin

Alternative name(s):
Brain-specific chordin-like protein
CR (chordin-like cysteine-rich) domain-containing adhesive protein
Short name=Cradin
von Willebrand factor C domain-containing protein 2

Gene names

Name:

Vwc2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	BMP antagonist which may play a role in neural development. Promotes cell adhesion. Ref.1 Ref.7
Subunit structure	Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including VWC2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing. Ref.8
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Cell junction › synapse Probable Ref.1 Ref.7 Ref.8.
Tissue specificity	Predominantly expressed in the brain (at protein level). It is expressed in the neurons but not the glial cells. Ref.1 Ref.7 Ref.8
Developmental stage	At 12.5 dpc, predominantly expressed in the developing diencephalon. At 16.5 dpc and 18.5 dpc, expressed in the brain, spinal cord, developing neural tubes and tongue but not in the cerebral cortex. At 16.5 dpc, present in developing oral and tooth germ epithelia (at protein level). Ref.1 Ref.7
Sequence similarities	Contains 2 VWFC domains.
Sequence caution	The sequence CAI25053.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Basement membrane Cell junction Extracellular matrix Secreted Synapse
Domain	Repeat Signal
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of BMP signaling pathway Inferred from direct assay Ref.1. Source: HGNC positive regulation of cell-substrate adhesion Inferred from direct assay Ref.7. Source: MGI positive regulation of neuron differentiation Inferred from direct assay Ref.1. Source: HGNC
Cellular_component	alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Inferred from direct assay Ref.8. Source: MGI basement membrane Inferred from direct assay Ref.7. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular space Inferred from direct assay Ref.1. Source: HGNC interstitial matrix Inferred from direct assay Ref.7. Source: MGI synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 324	297	Brorin		PRO_0000307160
Regions
	Domain	152 – 211	60	VWFC 1
	Domain	215 – 273	59	VWFC 2
	Motif	114 – 116	3	Mediates cell adhesion

Sequences

	Sequence		Length	Mass (Da)
	Q8C8N3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E6DD3FEB07F91DCB	FASTA	324	35,383
	10 20 30 40 50 60 MPSSSAMAVG ALSSSLLVTC CLMVALCSPS IPLEKLAQAP EQPGQEKREH ASRDSPGRVS 70 80 90 100 110 120 ELGRASRDEG SSARDWKSKG SRALSGREAW SKQKQAWAAQ GGSAKAADWQ VRPRGDTPQG 130 140 150 160 170 180 EPPAAAQEAI SLELVPTPEL PEEYAYPDYR GKGCVDESGF VYAIGEKFAP GPSACPCLCT 190 200 210 220 230 240 EEGPLCAQPE CPRLHPRCIH VDNSQCCPQC KEKKNYCEFR GKTYQTLEEF VVSPCERCRC 250 260 270 280 290 300 EANGEVLCTV SACPQTECVD PVYEPDQCCP ICKNGPNCFA ETAVIPAGRE VKTDECTICH 310 320 CTYEEGTWRI ERQAMCTRHE CRQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Brorin, a novel secreted bone morphogenetic protein antagonist, promotes neurogenesis in mouse neural precursor cells." Koike N., Kassai Y., Kouta Y., Miwa H., Konishi M., Itoh N. J. Biol. Chem. 282:15843-15850(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"A novel member of cysteine-rich protein family highly expressed in brain." Mochida Y., Yamauchi M. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Retina and Spinal cord.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Transcriptome-based systematic identification of extracellular matrix proteins." Manabe R., Tsutsui K., Yamada T., Kimura M., Nakano I., Shimono C., Sanzen N., Furutani Y., Fukuda T., Oguri Y., Shimamoto K., Kiyozumi D., Sato Y., Sado Y., Senoo H., Yamashina S., Fukuda S., Kawai J. , Sugiura N., Kimata K., Hayashizaki Y., Sekiguchi K. Proc. Natl. Acad. Sci. U.S.A. 105:12849-12854(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[8]	"High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes." Schwenk J., Harmel N., Brechet A., Zolles G., Berkefeld H., Muller C.S., Bildl W., Baehrens D., Huber B., Kulik A., Klocker N., Schulte U., Fakler B. Neuron 74:621-633(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN AMPAR COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB292670 mRNA. Translation: BAF51550.1. DQ421811 mRNA. Translation: ABD83334.1. AK044771 mRNA. Translation: BAC32080.1. AK141514 mRNA. Translation: BAE24710.1. AL663084, AL663071 Genomic DNA. Translation: CAI25053.2. Different initiation. AL663071, AL663084 Genomic DNA. Translation: CAI35949.1. CH466574 Genomic DNA. Translation: EDL40630.1. CH466574 Genomic DNA. Translation: EDL40631.1. BC120564 mRNA. Translation: AAI20565.1. BC137825 mRNA. Translation: AAI37826.1.
IPI	IPI00227179.
RefSeq	NP_796007.1. NM_177033.3.
UniGene	Mm.337851. Mm.487612.
3D structure databases
ProteinModelPortal	Q8C8N3.
SMR	Q8C8N3. Positions 153-211, 213-318.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000049692.
Proteomic databases
PRIDE	Q8C8N3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000056344; ENSMUSP00000049692; ENSMUSG00000050830. ENSMUST00000109681; ENSMUSP00000105303; ENSMUSG00000050830. ENSMUST00000129670; ENSMUSP00000128761; ENSMUSG00000050830.
GeneID	319922.
KEGG	mmu:319922.
Organism-specific databases
CTD	375567.
MGI	MGI:2442987. Vwc2.
Phylogenomic databases
eggNOG	NOG83498.
GeneTree	ENSGT00450000040255.
HOGENOM	HOG000036086.
HOVERGEN	HBG094842.
InParanoid	Q8C8N3.
OMA	SKQKQAW.
Gene expression databases
ArrayExpress	Q8C8N3.
Bgee	Q8C8N3.
CleanEx	MM_VWC2.
Genevestigator	Q8C8N3.
Family and domain databases
InterPro	IPR001007. VWF_C. [Graphical view]
Pfam	PF00093. VWC. 1 hit. [Graphical view]
SMART	SM00214. VWC. 2 hits. [Graphical view]
PROSITE	PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	395657.
SOURCE	Search...

Entry information

Entry name

VWC2_MOUSE

Accession

Primary (citable) accession number: Q8C8N3
Secondary accession number(s): B2RQ98, Q5SSH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program