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Q8C8N3 (VWC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Brorin
Alternative name(s):
Brain-specific chordin-like protein
CR (chordin-like cysteine-rich) domain-containing adhesive protein
Short name=Cradin
von Willebrand factor C domain-containing protein 2
Gene names
Name:Vwc2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

BMP antagonist which may play a role in neural development. Promotes cell adhesion. Ref.1 Ref.7

Subunit structure

Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including VWC2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing. Ref.8

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane. Cell junctionsynapse Probable Ref.1 Ref.7 Ref.8.

Tissue specificity

Predominantly expressed in the brain (at protein level). It is expressed in the neurons but not the glial cells. Ref.1 Ref.7 Ref.8

Developmental stage

At 12.5 dpc, predominantly expressed in the developing diencephalon. At 16.5 dpc and 18.5 dpc, expressed in the brain, spinal cord, developing neural tubes and tongue but not in the cerebral cortex. At 16.5 dpc, present in developing oral and tooth germ epithelia (at protein level). Ref.1 Ref.7

Sequence similarities

Contains 2 VWFC domains.

Sequence caution

The sequence CAI25053.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 324297Brorin
PRO_0000307160

Regions

Domain152 – 21160VWFC 1
Domain215 – 27359VWFC 2
Motif114 – 1163Mediates cell adhesion

Sequences

Sequence LengthMass (Da)Tools
Q8C8N3 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: E6DD3FEB07F91DCB

FASTA32435,383
        10         20         30         40         50         60 
MPSSSAMAVG ALSSSLLVTC CLMVALCSPS IPLEKLAQAP EQPGQEKREH ASRDSPGRVS 

        70         80         90        100        110        120 
ELGRASRDEG SSARDWKSKG SRALSGREAW SKQKQAWAAQ GGSAKAADWQ VRPRGDTPQG 

       130        140        150        160        170        180 
EPPAAAQEAI SLELVPTPEL PEEYAYPDYR GKGCVDESGF VYAIGEKFAP GPSACPCLCT 

       190        200        210        220        230        240 
EEGPLCAQPE CPRLHPRCIH VDNSQCCPQC KEKKNYCEFR GKTYQTLEEF VVSPCERCRC 

       250        260        270        280        290        300 
EANGEVLCTV SACPQTECVD PVYEPDQCCP ICKNGPNCFA ETAVIPAGRE VKTDECTICH 

       310        320 
CTYEEGTWRI ERQAMCTRHE CRQM 

« Hide

References

« Hide 'large scale' references
[1]"Brorin, a novel secreted bone morphogenetic protein antagonist, promotes neurogenesis in mouse neural precursor cells."
Koike N., Kassai Y., Kouta Y., Miwa H., Konishi M., Itoh N.
J. Biol. Chem. 282:15843-15850(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"A novel member of cysteine-rich protein family highly expressed in brain."
Mochida Y., Yamauchi M.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Retina and Spinal cord.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Transcriptome-based systematic identification of extracellular matrix proteins."
Manabe R., Tsutsui K., Yamada T., Kimura M., Nakano I., Shimono C., Sanzen N., Furutani Y., Fukuda T., Oguri Y., Shimamoto K., Kiyozumi D., Sato Y., Sado Y., Senoo H., Yamashina S., Fukuda S., Kawai J. expand/collapse author list , Sugiura N., Kimata K., Hayashizaki Y., Sekiguchi K.
Proc. Natl. Acad. Sci. U.S.A. 105:12849-12854(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[8]"High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes."
Schwenk J., Harmel N., Brechet A., Zolles G., Berkefeld H., Muller C.S., Bildl W., Baehrens D., Huber B., Kulik A., Klocker N., Schulte U., Fakler B.
Neuron 74:621-633(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AMPAR COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB292670 mRNA. Translation: BAF51550.1.
DQ421811 mRNA. Translation: ABD83334.1.
AK044771 mRNA. Translation: BAC32080.1.
AK141514 mRNA. Translation: BAE24710.1.
AL663084, AL663071 Genomic DNA. Translation: CAI25053.2. Different initiation.
AL663071, AL663084 Genomic DNA. Translation: CAI35949.1.
CH466574 Genomic DNA. Translation: EDL40630.1.
CH466574 Genomic DNA. Translation: EDL40631.1.
BC120564 mRNA. Translation: AAI20565.1.
BC137825 mRNA. Translation: AAI37826.1.
CCDSCCDS36111.1.
RefSeqNP_796007.1. NM_177033.3.
XP_006514796.1. XM_006514733.1.
UniGeneMm.337851.
Mm.487612.

3D structure databases

ProteinModelPortalQ8C8N3.
SMRQ8C8N3. Positions 152-211, 213-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000049692.

Proteomic databases

PRIDEQ8C8N3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000056344; ENSMUSP00000049692; ENSMUSG00000050830.
ENSMUST00000109681; ENSMUSP00000105303; ENSMUSG00000050830.
ENSMUST00000129670; ENSMUSP00000128761; ENSMUSG00000050830.
GeneID319922.
KEGGmmu:319922.
UCSCuc007iac.1. mouse.

Organism-specific databases

CTD375567.
MGIMGI:2442987. Vwc2.

Phylogenomic databases

eggNOGNOG83498.
GeneTreeENSGT00720000108792.
HOGENOMHOG000036086.
HOVERGENHBG094842.
InParanoidQ8C8N3.
OMASKQKQAW.
OrthoDBEOG73Z2TT.
PhylomeDBQ8C8N3.
TreeFamTF329913.

Gene expression databases

BgeeQ8C8N3.
CleanExMM_VWC2.
GenevestigatorQ8C8N3.

Family and domain databases

InterProIPR001007. VWF_C.
[Graphical view]
PfamPF00093. VWC. 1 hit.
[Graphical view]
SMARTSM00214. VWC. 2 hits.
[Graphical view]
PROSITEPS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio395657.
PROQ8C8N3.
SOURCESearch...

Entry information

Entry nameVWC2_MOUSE
AccessionPrimary (citable) accession number: Q8C8N3
Secondary accession number(s): B2RQ98, Q5SSH5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot