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Protein

Brorin

Gene

Vwc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

BMP antagonist which may play a role in neural development. Promotes cell adhesion.2 Publications

GO - Biological processi

  • negative regulation of BMP signaling pathway Source: HGNC
  • positive regulation of cell-substrate adhesion Source: MGI
  • positive regulation of neuron differentiation Source: HGNC
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Brorin
Alternative name(s):
Brain-specific chordin-like protein
CR (chordin-like cysteine-rich) domain-containing adhesive protein
Short name:
Cradin
von Willebrand factor C domain-containing protein 2
Gene namesi
Name:Vwc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2442987. Vwc2.

Subcellular locationi

GO - Cellular componenti

  • alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: MGI
  • basement membrane Source: MGI
  • cell junction Source: UniProtKB-KW
  • extracellular space Source: HGNC
  • interstitial matrix Source: MGI
  • proteinaceous extracellular matrix Source: MGI
  • synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Cell junction, Extracellular matrix, Secreted, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 324297BrorinPRO_0000307160Add
BLAST

Proteomic databases

PRIDEiQ8C8N3.

Expressioni

Tissue specificityi

Predominantly expressed in the brain (at protein level). It is expressed in the neurons but not the glial cells.3 Publications

Developmental stagei

At 12.5 dpc, predominantly expressed in the developing diencephalon. At 16.5 dpc and 18.5 dpc, expressed in the brain, spinal cord, developing neural tubes and tongue but not in the cerebral cortex. At 16.5 dpc, present in developing oral and tooth germ epithelia (at protein level).2 Publications

Gene expression databases

BgeeiQ8C8N3.
CleanExiMM_VWC2.
GenevisibleiQ8C8N3. MM.

Interactioni

Subunit structurei

Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including VWC2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049692.

Structurei

3D structure databases

ProteinModelPortaliQ8C8N3.
SMRiQ8C8N3. Positions 264-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 21160VWFC 1PROSITE-ProRule annotationAdd
BLAST
Domaini215 – 27359VWFC 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi114 – 1163Mediates cell adhesion

Sequence similaritiesi

Contains 2 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG83498.
GeneTreeiENSGT00720000108792.
HOGENOMiHOG000036086.
HOVERGENiHBG094842.
InParanoidiQ8C8N3.
OMAiSKQKQAW.
OrthoDBiEOG73Z2TT.
PhylomeDBiQ8C8N3.
TreeFamiTF329913.

Family and domain databases

InterProiIPR030111. Brorin.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR11339:SF258. PTHR11339:SF258. 1 hit.
PfamiPF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00214. VWC. 2 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8C8N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSSAMAVG ALSSSLLVTC CLMVALCSPS IPLEKLAQAP EQPGQEKREH
60 70 80 90 100
ASRDSPGRVS ELGRASRDEG SSARDWKSKG SRALSGREAW SKQKQAWAAQ
110 120 130 140 150
GGSAKAADWQ VRPRGDTPQG EPPAAAQEAI SLELVPTPEL PEEYAYPDYR
160 170 180 190 200
GKGCVDESGF VYAIGEKFAP GPSACPCLCT EEGPLCAQPE CPRLHPRCIH
210 220 230 240 250
VDNSQCCPQC KEKKNYCEFR GKTYQTLEEF VVSPCERCRC EANGEVLCTV
260 270 280 290 300
SACPQTECVD PVYEPDQCCP ICKNGPNCFA ETAVIPAGRE VKTDECTICH
310 320
CTYEEGTWRI ERQAMCTRHE CRQM
Length:324
Mass (Da):35,383
Last modified:March 1, 2003 - v1
Checksum:iE6DD3FEB07F91DCB
GO

Sequence cautioni

The sequence CAI25053.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB292670 mRNA. Translation: BAF51550.1.
DQ421811 mRNA. Translation: ABD83334.1.
AK044771 mRNA. Translation: BAC32080.1.
AK141514 mRNA. Translation: BAE24710.1.
AL663084, AL663071 Genomic DNA. Translation: CAI25053.2. Different initiation.
AL663071, AL663084 Genomic DNA. Translation: CAI35949.1.
CH466574 Genomic DNA. Translation: EDL40630.1.
CH466574 Genomic DNA. Translation: EDL40631.1.
BC120564 mRNA. Translation: AAI20565.1.
BC137825 mRNA. Translation: AAI37826.1.
CCDSiCCDS36111.1.
RefSeqiNP_796007.1. NM_177033.3.
XP_006514796.1. XM_006514733.2.
UniGeneiMm.337851.
Mm.487612.

Genome annotation databases

EnsembliENSMUST00000056344; ENSMUSP00000049692; ENSMUSG00000050830.
ENSMUST00000109681; ENSMUSP00000105303; ENSMUSG00000050830.
ENSMUST00000129670; ENSMUSP00000128761; ENSMUSG00000050830.
GeneIDi319922.
KEGGimmu:319922.
UCSCiuc007iac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB292670 mRNA. Translation: BAF51550.1.
DQ421811 mRNA. Translation: ABD83334.1.
AK044771 mRNA. Translation: BAC32080.1.
AK141514 mRNA. Translation: BAE24710.1.
AL663084, AL663071 Genomic DNA. Translation: CAI25053.2. Different initiation.
AL663071, AL663084 Genomic DNA. Translation: CAI35949.1.
CH466574 Genomic DNA. Translation: EDL40630.1.
CH466574 Genomic DNA. Translation: EDL40631.1.
BC120564 mRNA. Translation: AAI20565.1.
BC137825 mRNA. Translation: AAI37826.1.
CCDSiCCDS36111.1.
RefSeqiNP_796007.1. NM_177033.3.
XP_006514796.1. XM_006514733.2.
UniGeneiMm.337851.
Mm.487612.

3D structure databases

ProteinModelPortaliQ8C8N3.
SMRiQ8C8N3. Positions 264-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049692.

Proteomic databases

PRIDEiQ8C8N3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056344; ENSMUSP00000049692; ENSMUSG00000050830.
ENSMUST00000109681; ENSMUSP00000105303; ENSMUSG00000050830.
ENSMUST00000129670; ENSMUSP00000128761; ENSMUSG00000050830.
GeneIDi319922.
KEGGimmu:319922.
UCSCiuc007iac.1. mouse.

Organism-specific databases

CTDi375567.
MGIiMGI:2442987. Vwc2.

Phylogenomic databases

eggNOGiNOG83498.
GeneTreeiENSGT00720000108792.
HOGENOMiHOG000036086.
HOVERGENiHBG094842.
InParanoidiQ8C8N3.
OMAiSKQKQAW.
OrthoDBiEOG73Z2TT.
PhylomeDBiQ8C8N3.
TreeFamiTF329913.

Miscellaneous databases

NextBioi395657.
PROiQ8C8N3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C8N3.
CleanExiMM_VWC2.
GenevisibleiQ8C8N3. MM.

Family and domain databases

InterProiIPR030111. Brorin.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR11339:SF258. PTHR11339:SF258. 1 hit.
PfamiPF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00214. VWC. 2 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Brorin, a novel secreted bone morphogenetic protein antagonist, promotes neurogenesis in mouse neural precursor cells."
    Koike N., Kassai Y., Kouta Y., Miwa H., Konishi M., Itoh N.
    J. Biol. Chem. 282:15843-15850(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "A novel member of cysteine-rich protein family highly expressed in brain."
    Mochida Y., Yamauchi M.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Retina and Spinal cord.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  8. "High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes."
    Schwenk J., Harmel N., Brechet A., Zolles G., Berkefeld H., Muller C.S., Bildl W., Baehrens D., Huber B., Kulik A., Klocker N., Schulte U., Fakler B.
    Neuron 74:621-633(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN AMPAR COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVWC2_MOUSE
AccessioniPrimary (citable) accession number: Q8C8N3
Secondary accession number(s): B2RQ98, Q5SSH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2003
Last modified: June 24, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.