Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein SCAI

Gene

Scai

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.1 Publication

GO - Molecular functioni

  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor, Signal transduction inhibitor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SCAI
Alternative name(s):
Suppressor of cancer cell invasion protein
Gene namesi
Name:Scai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2443716. Scai.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • nuclear membrane Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Protein SCAIPRO_0000089736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphotyrosineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C8N2.
MaxQBiQ8C8N2.
PaxDbiQ8C8N2.
PeptideAtlasiQ8C8N2.
PRIDEiQ8C8N2.

PTM databases

iPTMnetiQ8C8N2.
PhosphoSiteiQ8C8N2.

Expressioni

Tissue specificityi

Expressed in most tissues tested with higher expression levels in brain, spleen and thymus.1 Publication

Gene expression databases

BgeeiQ8C8N2.
CleanExiMM_A930041I02RIK.
ExpressionAtlasiQ8C8N2. baseline and differential.
GenevisibleiQ8C8N2. MM.

Interactioni

Subunit structurei

Interacts with DIAPH1. Forms a nuclear ternary complex with MKL1 and SRF.1 Publication

Protein-protein interaction databases

BioGridi235895. 14 interactions.
IntActiQ8C8N2. 14 interactions.
STRINGi10090.ENSMUSP00000037194.

Structurei

3D structure databases

ProteinModelPortaliQ8C8N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 212212Necessary to inhibit MKL1-induced SRF transcriptional activityAdd
BLAST
Regioni71 – 173103Required for interaction with MKL1Add
BLAST

Sequence similaritiesi

Belongs to the SCAI family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEKH. Eukaryota.
ENOG410XRRG. LUCA.
GeneTreeiENSGT00390000009566.
HOGENOMiHOG000234356.
InParanoidiQ8C8N2.
OMAiCQDYLRK.
OrthoDBiEOG74BJRD.
PhylomeDBiQ8C8N2.
TreeFamiTF324872.

Family and domain databases

InterProiIPR022709. SCAI.
IPR016607. SCAI_metazoan/Viridiplantae.
[Graphical view]
PfamiPF12070. DUF3550. 1 hit.
[Graphical view]
PIRSFiPIRSF013022. UCP013022. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C8N2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRGARQSQQ PRSRLAPRLS GTVEKPPRKR KSRTEFTLKE TMSSGGAEDD
60 70 80 90 100
IPQGERKTVT DFCYLLDKSK QLFNGLRDLP QYGQKQWQSY FGRTFDVYTK
110 120 130 140 150
LWKFQQQHRQ VLDNRYGLKR WQIGEIASKI GQLYYHYYLR TSETSYLNEA
160 170 180 190 200
FSFYSAIRQR SYYSQVNKED RPELVVKKLR YYARFIVVCL LLNKMDVVKD
210 220 230 240 250
LVKELSDEIE DYTHRFNTED QVEWNLVLQE VAAFIEADPV MVLNDDNTIV
260 270 280 290 300
ITSNRLAETG APLLEQGMIV GQLSLADALI IGNCNNQVKF SELTVDMFRM
310 320 330 340 350
LQALEREPMN LASQMNKPGI QEPADKPTRR ENPHKYLLYK PTFSQLYTFL
360 370 380 390 400
AASFKELPAN SVLLIYLSAT GVFPTGRSDG EGPYDFGGVL TNSNRDIING
410 420 430 440 450
DAIHKRNQSH KEMHCLHPGD LYPFTRKPLF IVVDSSNSVA YKNFTNLFGQ
460 470 480 490 500
PLVCLLSPTA YPKALQDQSQ RGSLFTLFLN NPLMAFLFVS GLSSMRRGLW
510 520 530 540 550
EKCQEYLRKI NRDIAQLLTH SRSIDQAFLQ FFGDEFLRLL LTRFVFCSAT
560 570 580 590 600
MRMHKAFRET RNYPESYPQL PRDETVENPH LQKHILELAS ILDVRNIFFE

NSMDDY
Length:606
Mass (Da):70,275
Last modified:August 16, 2005 - v2
Checksum:i09CBEDC06FDC573F
GO
Isoform 2 (identifier: Q8C8N2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     322-325: EPAD → VIIA
     326-606: Missing.

Note: No experimental confirmation available.
Show »
Length:325
Mass (Da):37,814
Checksum:i302955B739C29075
GO

Sequence cautioni

The sequence BAC32904.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581T → A in BAC32081 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei322 – 3254EPAD → VIIA in isoform 2. 1 PublicationVSP_015121
Alternative sequencei326 – 606281Missing in isoform 2. 1 PublicationVSP_015122Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044772 mRNA. Translation: BAC32081.1.
AK046875 mRNA. Translation: BAC32904.1. Different initiation.
AK083280 mRNA. Translation: BAC38841.1.
AL844588, AL845350, AL928639 Genomic DNA. Translation: CAM46058.2.
AL928639, AL844588, AL845350 Genomic DNA. Translation: CAO77724.2.
AL845350, AL844588, AL928639 Genomic DNA. Translation: CAQ11836.1.
CH466519 Genomic DNA. Translation: EDL26843.1.
BC132485 mRNA. Translation: AAI32486.1.
CCDSiCCDS38121.1. [Q8C8N2-1]
RefSeqiNP_848893.2. NM_178778.3. [Q8C8N2-1]
UniGeneiMm.204836.

Genome annotation databases

EnsembliENSMUST00000038874; ENSMUSP00000037194; ENSMUSG00000035236. [Q8C8N2-1]
GeneIDi320271.
KEGGimmu:320271.
UCSCiuc008jof.1. mouse. [Q8C8N2-1]
uc008joh.1. mouse. [Q8C8N2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044772 mRNA. Translation: BAC32081.1.
AK046875 mRNA. Translation: BAC32904.1. Different initiation.
AK083280 mRNA. Translation: BAC38841.1.
AL844588, AL845350, AL928639 Genomic DNA. Translation: CAM46058.2.
AL928639, AL844588, AL845350 Genomic DNA. Translation: CAO77724.2.
AL845350, AL844588, AL928639 Genomic DNA. Translation: CAQ11836.1.
CH466519 Genomic DNA. Translation: EDL26843.1.
BC132485 mRNA. Translation: AAI32486.1.
CCDSiCCDS38121.1. [Q8C8N2-1]
RefSeqiNP_848893.2. NM_178778.3. [Q8C8N2-1]
UniGeneiMm.204836.

3D structure databases

ProteinModelPortaliQ8C8N2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235895. 14 interactions.
IntActiQ8C8N2. 14 interactions.
STRINGi10090.ENSMUSP00000037194.

PTM databases

iPTMnetiQ8C8N2.
PhosphoSiteiQ8C8N2.

Proteomic databases

EPDiQ8C8N2.
MaxQBiQ8C8N2.
PaxDbiQ8C8N2.
PeptideAtlasiQ8C8N2.
PRIDEiQ8C8N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038874; ENSMUSP00000037194; ENSMUSG00000035236. [Q8C8N2-1]
GeneIDi320271.
KEGGimmu:320271.
UCSCiuc008jof.1. mouse. [Q8C8N2-1]
uc008joh.1. mouse. [Q8C8N2-2]

Organism-specific databases

CTDi286205.
MGIiMGI:2443716. Scai.

Phylogenomic databases

eggNOGiENOG410IEKH. Eukaryota.
ENOG410XRRG. LUCA.
GeneTreeiENSGT00390000009566.
HOGENOMiHOG000234356.
InParanoidiQ8C8N2.
OMAiCQDYLRK.
OrthoDBiEOG74BJRD.
PhylomeDBiQ8C8N2.
TreeFamiTF324872.

Enzyme and pathway databases

ReactomeiR-MMU-5663220. RHO GTPases Activate Formins.

Miscellaneous databases

ChiTaRSiScai. mouse.
PROiQ8C8N2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C8N2.
CleanExiMM_A930041I02RIK.
ExpressionAtlasiQ8C8N2. baseline and differential.
GenevisibleiQ8C8N2. MM.

Family and domain databases

InterProiIPR022709. SCAI.
IPR016607. SCAI_metazoan/Viridiplantae.
[Graphical view]
PfamiPF12070. DUF3550. 1 hit.
[Graphical view]
PIRSFiPIRSF013022. UCP013022. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Hippocampus, Medulla oblongata and Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin."
    Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R.
    Nat. Cell Biol. 11:557-568(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DIAPH1; MKL1 AND SRF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiSCAI_MOUSE
AccessioniPrimary (citable) accession number: Q8C8N2
Secondary accession number(s): A2RTF5
, A3KGQ2, Q8C409, Q8C8K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: July 6, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.