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Q8C878 (UBA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NEDD8-activating enzyme E1 catalytic subunit

EC=6.3.2.-
Alternative name(s):
NEDD8-activating enzyme E1C
Ubiquitin-activating enzyme E1C
Ubiquitin-like modifier-activating enzyme 3
Short name=Ubiquitin-activating enzyme 3
Gene names
Name:Uba3
Synonyms:Ube1c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Ref.5

Enzyme regulation

Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity By similarity.

Pathway

Protein modification; protein neddylation.

Subunit structure

Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand By similarity. Interacts with TBATA. Ref.6

Involvement in disease

Defects in Uba3 are a cause of embryonic lethality. Mouse embryos deficient in Uba3 die at the preimplantation stage.

Miscellaneous

Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex By similarity.

Sequence similarities

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8C878-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8C878-2)

The sequence of this isoform differs from the canonical sequence as follows:
     396-462: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 462461NEDD8-activating enzyme E1 catalytic subunit
PRO_0000194942

Regions

Nucleotide binding100 – 12425ATP By similarity
Nucleotide binding148 – 17124ATP By similarity
Region53 – 7018Interaction with UBE2M N-terminus By similarity
Region157 – 1615Interaction with UBE2M N-terminus By similarity
Region192 – 21726Interaction with UBE2M N-terminus By similarity
Region227 – 2293Interaction with NEDD8 By similarity
Region242 – 2487Interaction with NAE1 By similarity
Region292 – 2954Interaction with NAE1 By similarity
Region331 – 3388Interaction with UBE2M N-terminus By similarity
Region352 – 3576Interaction with NEDD8 By similarity
Region368 – 46295Interaction with UBE2M core domain By similarity

Sites

Active site2371Glycyl thioester intermediate By similarity
Site2111Determines specificity for NEDD8 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Alternative sequence396 – 46267Missing in isoform 2.
VSP_010787

Experimental info

Sequence conflict1471K → E in BAB29346. Ref.1
Sequence conflict3341S → G in BAB29346. Ref.1
Sequence conflict3971M → I in BAC33258. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4034CE39E6E9A14E

FASTA46251,737
        10         20         30         40         50         60 
MADGEEPEKK RRRIEELLAE KMAVDGGCGD TGDWEGRWNH VKKFLERSGP FTHPDFEPST 

        70         80         90        100        110        120 
ESLQFLLDTC KVLVIGAGGL GCELLKNLAL SGFRQIHVID MDTIDVSNLN RQFLFRPKDV 

       130        140        150        160        170        180 
GRPKAEVAAE FLNDRVPNCN VVPHFNKIQD FNDTFYRQFH IIVCGLDSII ARRWINGMLI 

       190        200        210        220        230        240 
SLLNYEDGVL DPSSIVPLID GGTEGFKGNA RVILPGMTAC IECTLELYPP QVNFPMCTIA 

       250        260        270        280        290        300 
SMPRLPEHCI EYVRMLQWPK EQPFGDGVPL DGDDPEHIQW IFQKSIERAS QYNIRGVTYR 

       310        320        330        340        350        360 
LTQGVVKRII PAVASTNAVI AAVCATEVFK IATSAYIPLN NYLVFNDVDG LYTYTFEAER 

       370        380        390        400        410        420 
KENCPACSQL PQNIQFSPSA KLQEVLDYLT NSASLQMKSP AITATLEGKN RTLYLQSVTS 

       430        440        450        460 
IEERTRPNLS KTLKELGLVD GQELAVADVT TPQTVLFKLH FT 

« Hide

Isoform 2 [UniParc].

Checksum: 01E494E63C4166CB
Show »

FASTA39544,326

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryonic head, Kidney and Placenta.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-462 (ISOFORM 1).
[4]"Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway."
Gong L., Yeh E.T.H.
J. Biol. Chem. 274:12036-12042(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-462 (ISOFORM 1).
Tissue: Liver.
[5]"The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice."
Tateishi K., Omata M., Tanaka K., Chiba T.
J. Cell Biol. 155:571-580(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-462 (ISOFORM 1), FUNCTION.
Strain: C57BL/6.
[6]"Tbata modulates thymic stromal cell proliferation and thymus function."
Flomerfelt F.A., El Kassar N., Gurunathan C., Chua K.S., League S.C., Schmitz S., Gershon T.R., Kapoor V., Yan X.Y., Schwartz R.H., Gress R.E.
J. Exp. Med. 207:2521-2532(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TBATA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK014433 mRNA. Translation: BAB29346.1.
AK032514 mRNA. Translation: BAC27905.1.
AK048148 mRNA. Translation: BAC33258.1.
AK159381 mRNA. Translation: BAE35036.1.
AK168859 mRNA. Translation: BAE40680.1.
CH466523 Genomic DNA. Translation: EDK99352.1.
BC002002 mRNA. Translation: AAH02002.1.
AF077330 mRNA. Translation: AAC27323.1.
AY029181 mRNA. Translation: AAK33015.1.
RefSeqNP_001104576.1. NM_001111106.1.
NP_035796.2. NM_011666.2.
XP_006505974.1. XM_006505911.1.
XP_006505975.1. XM_006505912.1.
XP_006505976.1. XM_006505913.1.
UniGeneMm.277626.

3D structure databases

ProteinModelPortalQ8C878.
SMRQ8C878. Positions 33-462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204409. 2 interactions.

PTM databases

PhosphoSiteQ8C878.

Proteomic databases

PaxDbQ8C878.
PRIDEQ8C878.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000089287; ENSMUSP00000086701; ENSMUSG00000030061. [Q8C878-1]
GeneID22200.
KEGGmmu:22200.
UCSCuc009dam.2. mouse. [Q8C878-1]

Organism-specific databases

CTD9039.
MGIMGI:1341217. Uba3.

Phylogenomic databases

eggNOGCOG0476.
GeneTreeENSGT00550000074831.
HOGENOMHOG000166793.
HOVERGENHBG082736.
InParanoidQ3TG68.
KOK10686.
OMADHIQWIF.
TreeFamTF300499.

Enzyme and pathway databases

UniPathwayUPA00885.

Gene expression databases

ArrayExpressQ8C878.
BgeeQ8C878.
CleanExMM_UBA3.
GenevestigatorQ8C878.

Family and domain databases

Gene3D1.10.10.520. 1 hit.
3.10.20.260. 1 hit.
3.40.50.720. 2 hits.
InterProIPR014929. E2_binding.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR023318. Ub_act_enz_dom_a.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018074. UBQ-activ_enz_E1_AS.
[Graphical view]
PfamPF08825. E2_bind. 1 hit.
PF00899. ThiF. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 1 hit.
[Graphical view]
SUPFAMSSF69572. SSF69572. 1 hit.
PROSITEPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302185.
PROQ8C878.
SOURCESearch...

Entry information

Entry nameUBA3_MOUSE
AccessionPrimary (citable) accession number: Q8C878
Secondary accession number(s): O88598, Q3TG68, Q9D6B7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot