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Protein

Myelin expression factor 2

Gene

Myef2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin expression factor 2
Short name:
MEF-2
Short name:
MyEF-2
Gene namesi
Name:Myef2
Synonyms:Kiaa1341, Mef2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 2, Chromosome 3

Organism-specific databases

MGIiMGI:104592. Myef2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Myelin expression factor 2PRO_0000081656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei422 – 4221PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C854.
MaxQBiQ8C854.
PaxDbiQ8C854.
PeptideAtlasiQ8C854.
PRIDEiQ8C854.

PTM databases

iPTMnetiQ8C854.
PhosphoSiteiQ8C854.
SwissPalmiQ8C854.

Expressioni

Tissue specificityi

Highly expressed in the brain.1 Publication

Gene expression databases

BgeeiQ8C854.
ExpressionAtlasiQ8C854. baseline and differential.
GenevisibleiQ8C854. MM.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi201640. 4 interactions.
IntActiQ8C854. 5 interactions.
MINTiMINT-4118183.
STRINGi10090.ENSMUSP00000123088.

Structurei

3D structure databases

ProteinModelPortaliQ8C854.
SMRiQ8C854. Positions 93-167, 217-308, 510-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 16979RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini224 – 30178RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini514 – 59077RRM 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi321 – 513193Gly-richAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IKNU. Eukaryota.
ENOG4110PAX. LUCA.
GeneTreeiENSGT00410000025635.
HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiQ8C854.
OMAiHEEYRSH.
OrthoDBiEOG7ZSHSK.
PhylomeDBiQ8C854.
TreeFamiTF313406.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C854-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADADKSEAA AGDDGSQQQP AEPRRDTHPG EPEKPPRSSA NGVKMENDES
60 70 80 90 100
VKEEKSDLKE KSTGNKKANR FHPYSKDKNS GTGEKKGPNR NRVFISNIPY
110 120 130 140 150
DMKWQAIKDL MREKVGEVTY VELFKDAEGK SRGCGVVEFK DEEFVKKALE
160 170 180 190 200
TMNKYDLSGR PLNIKEDPDG ENARRALQRT GTSFQGSHAS DVGSGLVNLP
210 220 230 240 250
PSILNNPNIP PEVISNLQAG RLGSTIFVAN LDFKVGWKKL KEVFSIAGTV
260 270 280 290 300
KRADIKEDKD GKSRGMGTVT FEQAIEAVQA ISMFNGQFLF DRPMHVKMDD
310 320 330 340 350
KSVPHEDYRS HDSKTSQLPR GLGGIGMGLG PGGQPISASQ LNITGVMGNL
360 370 380 390 400
GPSGMGMDGP GFGGVNRIGG GVGFGGLEAM NSMAGFGGVG RMGELYRGAM
410 420 430 440 450
TSSMERDFGR GDIGLSRGFG DSFGRLGSAM IGGFAGRIGA SNMGPVGTGI
460 470 480 490 500
SGSMSGMSTV TGGMGMGLDR MSSSFDRMGP GIGAILERSI DVDRGFLSGP
510 520 530 540 550
MGSGMRDRLG SKGNQIFVRN LPFDLTWQKL KEKFSQCGHV MFAEIKMENG
560 570 580 590
KSKGCGTVRF ESAESAEKAC RIMNGIKISG REIDVRLDRN A
Length:591
Mass (Da):63,295
Last modified:March 1, 2003 - v1
Checksum:i7F30703C519DB9A6
GO
Isoform 2 (identifier: Q8C854-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     354-370: Missing.

Show »
Length:574
Mass (Da):61,734
Checksum:iFC3A44B015C32E86
GO
Isoform 3 (identifier: Q8C854-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     427-451: GSAMIGGFAGRIGASNMGPVGTGIS → G
     511-512: SK → RN
     513-591: Missing.

Show »
Length:488
Mass (Da):52,213
Checksum:i797C87A2C49DF8CC
GO
Isoform 4 (identifier: Q8C854-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     394-451: ELYRGAMTSSMERDFGRGDIGLSRGFGDSFGRLGSAMIGGFAGRIGASNMGPVGTGIS → G
     539-555: HVMFAEIKMENGKSKGC → QINRDSKTHSNCGQHHS
     556-591: Missing.

Show »
Length:498
Mass (Da):53,544
Checksum:iEB086DAAA1D41429
GO

Sequence cautioni

The sequence AAH60946.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC98146.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411L → R in AAA78270 (PubMed:7534248).Curated
Sequence conflicti537 – 5371C → L in AAA78270 (PubMed:7534248).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei354 – 37017Missing in isoform 2. 2 PublicationsVSP_013455Add
BLAST
Alternative sequencei394 – 45158ELYRG…GTGIS → G in isoform 4. 1 PublicationVSP_013456Add
BLAST
Alternative sequencei427 – 45125GSAMI…GTGIS → G in isoform 3. 1 PublicationVSP_013457Add
BLAST
Alternative sequencei511 – 5122SK → RN in isoform 3. 1 PublicationVSP_013458
Alternative sequencei513 – 59179Missing in isoform 3. 1 PublicationVSP_013459Add
BLAST
Alternative sequencei539 – 55517HVMFA…KSKGC → QINRDSKTHSNCGQHHS in isoform 4. 1 PublicationVSP_013460Add
BLAST
Alternative sequencei556 – 59136Missing in isoform 4. 1 PublicationVSP_013461Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048404 mRNA. Translation: BAC33325.1.
AK129336 mRNA. Translation: BAC98146.1. Different initiation.
BC060946 mRNA. Translation: AAH60946.1. Different initiation.
AF483504 mRNA. Translation: AAL90778.1.
AF483505 mRNA. Translation: AAL90779.1.
U13262 mRNA. Translation: AAA78270.1.
CCDSiCCDS50690.1. [Q8C854-3]
CCDS50691.1. [Q8C854-4]
PIRiA56704.
RefSeqiNP_001155889.1. NM_001162417.1. [Q8C854-4]
XP_006498947.1. XM_006498884.2. [Q8C854-3]
XP_011237644.1. XM_011239342.1. [Q8C854-4]
UniGeneiMm.18535.
Mm.330538.
Mm.455632.

Genome annotation databases

EnsembliENSMUST00000061419; ENSMUSP00000058811; ENSMUSG00000049230. [Q8C854-2]
ENSMUST00000067780; ENSMUSP00000066312; ENSMUSG00000027201. [Q8C854-3]
ENSMUST00000110501; ENSMUSP00000106127; ENSMUSG00000027201. [Q8C854-3]
ENSMUST00000152367; ENSMUSP00000123088; ENSMUSG00000027201. [Q8C854-4]
GeneIDi17876.
KEGGimmu:17876.
UCSCiuc008mbw.2. mouse. [Q8C854-4]
uc008mby.2. mouse. [Q8C854-3]
uc012cdb.1. mouse. [Q8C854-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048404 mRNA. Translation: BAC33325.1.
AK129336 mRNA. Translation: BAC98146.1. Different initiation.
BC060946 mRNA. Translation: AAH60946.1. Different initiation.
AF483504 mRNA. Translation: AAL90778.1.
AF483505 mRNA. Translation: AAL90779.1.
U13262 mRNA. Translation: AAA78270.1.
CCDSiCCDS50690.1. [Q8C854-3]
CCDS50691.1. [Q8C854-4]
PIRiA56704.
RefSeqiNP_001155889.1. NM_001162417.1. [Q8C854-4]
XP_006498947.1. XM_006498884.2. [Q8C854-3]
XP_011237644.1. XM_011239342.1. [Q8C854-4]
UniGeneiMm.18535.
Mm.330538.
Mm.455632.

3D structure databases

ProteinModelPortaliQ8C854.
SMRiQ8C854. Positions 93-167, 217-308, 510-591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201640. 4 interactions.
IntActiQ8C854. 5 interactions.
MINTiMINT-4118183.
STRINGi10090.ENSMUSP00000123088.

PTM databases

iPTMnetiQ8C854.
PhosphoSiteiQ8C854.
SwissPalmiQ8C854.

Proteomic databases

EPDiQ8C854.
MaxQBiQ8C854.
PaxDbiQ8C854.
PeptideAtlasiQ8C854.
PRIDEiQ8C854.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061419; ENSMUSP00000058811; ENSMUSG00000049230. [Q8C854-2]
ENSMUST00000067780; ENSMUSP00000066312; ENSMUSG00000027201. [Q8C854-3]
ENSMUST00000110501; ENSMUSP00000106127; ENSMUSG00000027201. [Q8C854-3]
ENSMUST00000152367; ENSMUSP00000123088; ENSMUSG00000027201. [Q8C854-4]
GeneIDi17876.
KEGGimmu:17876.
UCSCiuc008mbw.2. mouse. [Q8C854-4]
uc008mby.2. mouse. [Q8C854-3]
uc012cdb.1. mouse. [Q8C854-2]

Organism-specific databases

CTDi50804.
MGIiMGI:104592. Myef2.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IKNU. Eukaryota.
ENOG4110PAX. LUCA.
GeneTreeiENSGT00410000025635.
HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiQ8C854.
OMAiHEEYRSH.
OrthoDBiEOG7ZSHSK.
PhylomeDBiQ8C854.
TreeFamiTF313406.

Miscellaneous databases

PROiQ8C854.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C854.
ExpressionAtlasiQ8C854. baseline and differential.
GenevisibleiQ8C854. MM.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Embryonic tail.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 36-591 (ISOFORM 4).
    Tissue: Testis.
  4. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
    Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
    Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-591 (ISOFORM 2).
    Strain: ILS and ISS.
  5. "Regulation of mouse myelin basic protein gene transcription by a sequence-specific single-stranded DNA-binding protein in vitro."
    Steplewski A., Haas S., Amini S., Khalili K.
    Gene 154:215-218(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 140-591 (ISOFORM 2), FUNCTION IN TRANSCRIPTIONAL REPRESSION, DNA-BINDING.
  6. "Identification of a sequence-specific single-stranded DNA binding protein that suppresses transcription of the mouse myelin basic protein gene."
    Haas S., Steplewski A., Siracusa L.D., Amini S., Khalili K.
    J. Biol. Chem. 270:12503-12510(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION, DNA-BINDING, TISSUE SPECIFICITY.
  7. "Evidence for inhibition of MyEF-2 binding to MBP promoter by MEF-1/Pur alpha."
    Muralidharan V., Tretiakova A., Steplewski A., Haas S., Amini S., Johnson E., Khalili K.
    J. Cell. Biochem. 66:524-531(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION, SUBUNIT.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung, Spleen and Testis.

Entry informationi

Entry nameiMYEF2_MOUSE
AccessioniPrimary (citable) accession number: Q8C854
Secondary accession number(s): Q60690
, Q6P930, Q6ZPT3, Q8QZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.